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The map label for this gene is murI

Identifier: 73541920

GI number: 73541920

Start: 2465188

End: 2466006

Strand: Direct

Name: murI

Synonym: Reut_A2233

Alternate gene names: 73541920

Gene position: 2465188-2466006 (Clockwise)

Preceding gene: 73541919

Following gene: 73541922

Centisome position: 64.76

GC content: 65.57

Gene sequence:

>819_bases
TTGCCTAACGTGAACCCCGCACCCATCGGCGTCTTCGATTCCGGCCTCGGCGGCCTGTCCGTGCTGCGCGAGATCCGCGC
GCTGCTGCCGCATGAATCGCTGCTCTACCTGGCCGACTCGAAGTACGCGCCCTATGGCGAGAAACCGGAAGCTTTCGTGC
AGGCGCGTACGCTGCAGGCGTGCGAGTGGCTGCTCGCGCAAGGCTGCAAGGCTCTGGTGATCGCGTGCAACACTGCCACC
GGCCATGCCGTGGAGCTGCTGCGCCAAACACTTCCCGTGCCGATCATCGGCGTAGAACCGGGACTCAAGCCGGCTGCGGC
GGCCAGCCAAAGCAAAGTCGTGGGCGTGCTGGCCACGGCCAACACGCTCAAGAGCGGCAAGTTCGCGCGGCTGCTGGCAT
CACTCGACGGCGAAAGCCGCTTTATCTGCGAAGCGGGACTGGGCCTTGTGCCACTGATCGAGCAAGGCGACATCGATGGC
CCCGACATCCGCGGGCGGCTCGACAATTACCTCACGCCGATGCTCGAGGCCGGCGCCGACACACTGGTACTCGGCTGCAC
TCACTACCCTTTCCTGTCCGACACCATCCGCGACATGGTCGGCAATCAGCTGACACTGGTGGATACGGGCAGCGCGATCG
CACGTCAGCTGGCGCGCAAGCTCGTCGAGCACGATCTTGCCGTTGCGCCCGGCGCCGTGCCGCAAGACCGCTTCGTCTCG
ACCAAGGATGCCGCCCACCTCAGGCAGATGGCCGCCGCGCTGCTGCATATCGAAACGCAGGCGGAAACGGTAGCCATCGA
ACCCGCCCCGGCTTTCTGA

Upstream 100 bases:

>100_bases
GGCCAAGATCGGCAAGATTCCGGTCGCGGCGCTGTAAGATTGCGCATCTGCATAAGAAAGCCGGGCTCCGCCCGGCTTTT
TTCCATCCCCGCCCTTCTGC

Downstream 100 bases:

>100_bases
CGCCTATCGCAGGAACCCCACCGGACGGTGCGCGCGCACGTCTGGCTTGACTGCATGCTCATGGCGCAACTGCGCGAGGA
ATTCCTCTGGCGTCAGTGAG

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPNVNPAPIGVFDSGLGGLSVLREIRALLPHESLLYLADSKYAPYGEKPEAFVQARTLQACEWLLAQGCKALVIACNTAT
GHAVELLRQTLPVPIIGVEPGLKPAAAASQSKVVGVLATANTLKSGKFARLLASLDGESRFICEAGLGLVPLIEQGDIDG
PDIRGRLDNYLTPMLEAGADTLVLGCTHYPFLSDTIRDMVGNQLTLVDTGSAIARQLARKLVEHDLAVAPGAVPQDRFVS
TKDAAHLRQMAAALLHIETQAETVAIEPAPAF

Sequences:

>Translated_272_residues
MPNVNPAPIGVFDSGLGGLSVLREIRALLPHESLLYLADSKYAPYGEKPEAFVQARTLQACEWLLAQGCKALVIACNTAT
GHAVELLRQTLPVPIIGVEPGLKPAAAASQSKVVGVLATANTLKSGKFARLLASLDGESRFICEAGLGLVPLIEQGDIDG
PDIRGRLDNYLTPMLEAGADTLVLGCTHYPFLSDTIRDMVGNQLTLVDTGSAIARQLARKLVEHDLAVAPGAVPQDRFVS
TKDAAHLRQMAAALLHIETQAETVAIEPAPAF
>Mature_271_residues
PNVNPAPIGVFDSGLGGLSVLREIRALLPHESLLYLADSKYAPYGEKPEAFVQARTLQACEWLLAQGCKALVIACNTATG
HAVELLRQTLPVPIIGVEPGLKPAAAASQSKVVGVLATANTLKSGKFARLLASLDGESRFICEAGLGLVPLIEQGDIDGP
DIRGRLDNYLTPMLEAGADTLVLGCTHYPFLSDTIRDMVGNQLTLVDTGSAIARQLARKLVEHDLAVAPGAVPQDRFVST
KDAAHLRQMAAALLHIETQAETVAIEPAPAF

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=230, Percent_Identity=39.1304347826087, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_CUPPJ (Q46Z37)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296440.1
- HSSP:   P94556
- ProteinModelPortal:   Q46Z37
- SMR:   Q46Z37
- GeneID:   3610203
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2233
- NMPDR:   fig|264198.3.peg.2836
- HOGENOM:   HBG645102
- OMA:   ARTNAMI
- ProtClustDB:   PRK00865
- BioCyc:   REUT264198:REUT_A2233-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28760; Mature: 28628

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNVNPAPIGVFDSGLGGLSVLREIRALLPHESLLYLADSKYAPYGEKPEAFVQARTLQA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
CEWLLAQGCKALVIACNTATGHAVELLRQTLPVPIIGVEPGLKPAAAASQSKVVGVLATA
HHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCEEEEEEEH
NTLKSGKFARLLASLDGESRFICEAGLGLVPLIEQGDIDGPDIRGRLDNYLTPMLEAGAD
HHHCCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
TLVLGCTHYPFLSDTIRDMVGNQLTLVDTGSAIARQLARKLVEHDLAVAPGAVPQDRFVS
EEEEECCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC
TKDAAHLRQMAAALLHIETQAETVAIEPAPAF
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure 
PNVNPAPIGVFDSGLGGLSVLREIRALLPHESLLYLADSKYAPYGEKPEAFVQARTLQA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
CEWLLAQGCKALVIACNTATGHAVELLRQTLPVPIIGVEPGLKPAAAASQSKVVGVLATA
HHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCCHHHCCCCCEEEEEEEH
NTLKSGKFARLLASLDGESRFICEAGLGLVPLIEQGDIDGPDIRGRLDNYLTPMLEAGAD
HHHCCCHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCC
TLVLGCTHYPFLSDTIRDMVGNQLTLVDTGSAIARQLARKLVEHDLAVAPGAVPQDRFVS
EEEEECCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC
TKDAAHLRQMAAALLHIETQAETVAIEPAPAF
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA