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The map label for this gene is fumB [H]

Identifier: 73541919

GI number: 73541919

Start: 2463601

End: 2465124

Strand: Direct

Name: fumB [H]

Synonym: Reut_A2232

Alternate gene names: 73541919

Gene position: 2463601-2465124 (Clockwise)

Preceding gene: 73541918

Following gene: 73541920

Centisome position: 64.72

GC content: 64.76

Gene sequence:

>1524_bases
ATGACCGTCATCAAGCAAGAAGACCTCATCCAGAGCGTCGCAGACTCCCTGCAGTACATCAGCTACTACCACCCGATGGA
CTACATCACCAGCCTGGGCCGTGCCTATGAGCTGGAGCAGAGTCCCGCGGCCAAGGATGCGATCGCGCAGATCCTGACCA
ACAGCCGCATGTGCGCGGAAGGCAAGCGCCCCATCTGTCAGGACACCGGCATCGTCACGGTCTTCGTCAAGGTGGGCATG
GACGTGCGCTGGGATGGCGCCACCATGGGCCTGACCGACATGATCAACGAAGGCGTGCGCCGCGGCTATACGCACCCGGA
CAACGTGCTGCGCGCGTCGATCGTCAGCCCGCCCGAAGGTGGCCGCAAGAACACCAAGGACAACACCCCGGCCGTGATCC
ACTACGAAGTGGTGCCGGGCAACACGGTGGACATCCAGGTCGCGGCCAAGGGCGGCGGCTCGGAGAACAAGTCCAAGTTC
GTGATGCTGAACCCGTCCGACTCGATCGTTGACTGGGTGCTGAAGACCGTGCCGACCATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTGGGCATCGGCATCGGCGGTACCGCCGAGAAGGCCATGGTCATGGCCAAGGAATCGCTGATGGAGTCCATCG
ACATCCAGGACATCATCGCTCGTGGTCCGAAGGACTGGGTCGAGGAACTGCGCGTCGAGCTCTACGAGAAGGTCAACGCG
CTGGGCATTGGCGCGCAAGGCCTGGGCGGCCTGGCCACCGTGCTGGACGTCAAGATCATGGCCTGCCCGACGCACGCTGC
GTCCAAGCCGGTCGCGATGATCCCGAACTGCGCAGCCACCCGCCACGTGCACTTCACGCTGGACGGCAGCGGCCCGGCCA
AGCTGGAAGCGCCGGACCTGTCGCAATGGCCGAAGGTCGAGTGGGCGCCTAACACCGAAACGTCGAAGCGCGTCGACCTG
AACACGCTGACGCCGGAAGAAGTCGCCTCGTGGAAGCCGGGCCAGACCCTGCTGCTCAACGGCAAGATGCTGACCGGCCG
CGACGCCGCGCACAAGCGCATCGCCGACATGCTGGCCAAGGGCGAAAAGCTGCCGGTGGACTTCAAGAACCGCGTGATCT
ACTACGTCGGCCCGGTTGACCCGGTCCGTGACGAGGCCGTCGGCCCGGCAGGCCCCACCACCGCCACACGCATGGACAAG
TTCACCGAGATGATGCTGGCGGAAACCGGCCTGATCTCGATGATCGGCAAGGCCGAGCGCGGCCCGGTAGCCATCGAAGC
CATCAAGAAGCACAAGTCGGCTTACCTGATGGCCGTGGGCGGCGCCGCTTACCTGGTGGCCAAGGCCATCAAGGAAGCCA
AGGTCGTCGGCTTCGAAGACCTCGGCATGGAAGCCATCTACGAGTTCGACGTCAAGGACATGCCCGTGACCGTCGCCGTG
GACAGCCAAGGCACTTCGGTCCACAAGACCGGCCCTGCGGAATGGCAGGCCAAGATCGGCAAGATTCCGGTCGCGGCGCT
GTAA

Upstream 100 bases:

>100_bases
TGCACGGCGAGCCGTGTAGACGCCTGTATGCAGCCGGGCAGAGCGCCCCGGCTCGGTGTATGATTTCGGGCTGTGTTTCA
CCGCTGCCACCCCCCACAAA

Downstream 100 bases:

>100_bases
GATTGCGCATCTGCATAAGAAAGCCGGGCTCCGCCCGGCTTTTTTCCATCCCCGCCCTTCTGCTTGCCTAACGTGAACCC
CGCACCCATCGGCGTCTTCG

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGM
DVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEVVPGNTVDIQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMESIDIQDIIARGPKDWVEELRVELYEKVNA
LGIGAQGLGGLATVLDVKIMACPTHAASKPVAMIPNCAATRHVHFTLDGSGPAKLEAPDLSQWPKVEWAPNTETSKRVDL
NTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPVDFKNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTEMMLAETGLISMIGKAERGPVAIEAIKKHKSAYLMAVGGAAYLVAKAIKEAKVVGFEDLGMEAIYEFDVKDMPVTVAV
DSQGTSVHKTGPAEWQAKIGKIPVAAL

Sequences:

>Translated_507_residues
MTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGM
DVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEVVPGNTVDIQVAAKGGGSENKSKF
VMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMESIDIQDIIARGPKDWVEELRVELYEKVNA
LGIGAQGLGGLATVLDVKIMACPTHAASKPVAMIPNCAATRHVHFTLDGSGPAKLEAPDLSQWPKVEWAPNTETSKRVDL
NTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPVDFKNRVIYYVGPVDPVRDEAVGPAGPTTATRMDK
FTEMMLAETGLISMIGKAERGPVAIEAIKKHKSAYLMAVGGAAYLVAKAIKEAKVVGFEDLGMEAIYEFDVKDMPVTVAV
DSQGTSVHKTGPAEWQAKIGKIPVAAL
>Mature_506_residues
TVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQDTGIVTVFVKVGMD
VRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVSPPEGGRKNTKDNTPAVIHYEVVPGNTVDIQVAAKGGGSENKSKFV
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMVMAKESLMESIDIQDIIARGPKDWVEELRVELYEKVNAL
GIGAQGLGGLATVLDVKIMACPTHAASKPVAMIPNCAATRHVHFTLDGSGPAKLEAPDLSQWPKVEWAPNTETSKRVDLN
TLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAKGEKLPVDFKNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKF
TEMMLAETGLISMIGKAERGPVAIEAIKKHKSAYLMAVGGAAYLVAKAIKEAKVVGFEDLGMEAIYEFDVKDMPVTVAVD
SQGTSVHKTGPAEWQAKIGKIPVAAL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=467, Percent_Identity=32.762312633833, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790564, Length=466, Percent_Identity=30.68669527897, Blast_Score=198, Evalue=8e-52,
Organism=Escherichia coli, GI1789442, Length=277, Percent_Identity=28.8808664259928, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=27.5280898876405, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54539; Mature: 54407

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEQSPAAKDAIAQILTNSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFVKVGMDVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVSPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHEEEECCCCCC
GRKNTKDNTPAVIHYEVVPGNTVDIQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMVMAKESLMESIDIQDIIARGPKDWVEELRVELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMACPTHAASKPVAMIPNCAATRHVHFTLDGSGPAKLEAPDL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCEEEEEEEECCCCCCEECCCCC
SQWPKVEWAPNTETSKRVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEKLPVDFKNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAETGLISMIGKAER
CCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCC
GPVAIEAIKKHKSAYLMAVGGAAYLVAKAIKEAKVVGFEDLGMEAIYEFDVKDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCHHHCCCHHEEEECCCCCCEEEEE
DSQGTSVHKTGPAEWQAKIGKIPVAAL
CCCCCEEECCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVIKQEDLIQSVADSLQYISYYHPMDYITSLGRAYELEQSPAAKDAIAQILTNSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHC
GKRPICQDTGIVTVFVKVGMDVRWDGATMGLTDMINEGVRRGYTHPDNVLRASIVSPPEG
CCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCHHHHEEEECCCCCC
GRKNTKDNTPAVIHYEVVPGNTVDIQVAAKGGGSENKSKFVMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMVMAKESLMESIDIQDIIARGPKDWVEELRVELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHH
LGIGAQGLGGLATVLDVKIMACPTHAASKPVAMIPNCAATRHVHFTLDGSGPAKLEAPDL
EECCCCCCCHHHHHHEEEEEECCCCCCCCCEEECCCCCCEEEEEEEECCCCCCEECCCCC
SQWPKVEWAPNTETSKRVDLNTLTPEEVASWKPGQTLLLNGKMLTGRDAAHKRIADMLAK
CCCCCCEECCCCCCCCEEEECCCCHHHHHCCCCCCEEEEECEEECCCHHHHHHHHHHHHC
GEKLPVDFKNRVIYYVGPVDPVRDEAVGPAGPTTATRMDKFTEMMLAETGLISMIGKAER
CCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCC
GPVAIEAIKKHKSAYLMAVGGAAYLVAKAIKEAKVVGFEDLGMEAIYEFDVKDMPVTVAV
CCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCHHHCCCHHEEEECCCCCCEEEEE
DSQGTSVHKTGPAEWQAKIGKIPVAAL
CCCCCEEECCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]