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The map label for this gene is yfiN [H]

Identifier: 73541909

GI number: 73541909

Start: 2452780

End: 2454054

Strand: Direct

Name: yfiN [H]

Synonym: Reut_A2222

Alternate gene names: 73541909

Gene position: 2452780-2454054 (Clockwise)

Preceding gene: 73541908

Following gene: 73541910

Centisome position: 64.44

GC content: 66.27

Gene sequence:

>1275_bases
ATGAAGCCCGGATCGAGGCCCGCGGGCGGCCGACAACGCCCTACCCTGTACAGCACGCTGCGACGCATTCATCTTGGCGT
AGCGCTGCTGGCGGTCTGCACGGCGGGCCTCTCCCTGACCGCGCTCGGCTTTGCTGCACTTCGGGTCTATGCGGACCACA
ACCTGCGCCTGGTGGCACGGTCGATCTCCTATACGCTCGAAGCGCCGGTGGTATTCCGCGACAAGGTCGCCACTGGCGAG
ACGCTGGCCGTGATTGCCGCGTCCGAAGACGTCTCCCGCGCTACCGTCCTCGATCTCGACAACCGCGTGCTGGCCGACTG
GCAGCGTCCCGGCCACGAACGCCGAGACGGCTTGCGGTACGCGCTCGGCGAATTGCTCAGCACCGGCGTGATCGAGCAGC
CGATCCTGCACGACACCCAGCCGGTCGGTACCCTGCGGATGACCGGCGATCCGTCCAATCTGCTGCAGTTCCTGCTGCAA
GGCGCGCTGGGCCTGCTAGCCTGCCTGCTGCTGACCGCCGTCAGCGCGCACTATATGTCGAGCCGCATGCTCGCGCAGAT
CGTCGAGCCGTTGCGCAACCTGATGCGCGTGGCTCACGCCGTACGTTCGGAGCGCGACTTCGCCCAGCGCACCCCCCCCG
CCCGCATCGAGGAACTGAATGCACTTGGCGACGACTTCAACGGCCTGCTCGATGAGCTGGAAACGTGGCAAAACCAGATG
CAGCATGAGAACCGCTCGCTCGCGCACCGTGCCAGCCATGACTCGCTGACCGGCCTGCTCAACCGGTCCGCGTTTGAAGA
ACTGCTTGAGCTTGCCGTTCGCGCCGCGGCTGCCAGCGCTAGCTATGCGGCATTGCTCTATCTCGACAGCGACGACTTCA
AAGGCATCAACGACCGGTTCGGCCATGCCGCCGGAGACGTGGTGCTGGTCGCGCTGGCTCAGCGCATCCGAGCAAGCGTG
CGCGAAGGCGATGTGGTCGCGCGCCTTGGCGGCGACGAGTTTGCGGTGCTCGTCACGTCCTTGCGCGCTGCGGCGGATAT
CGTGCCGATTGCCGAGCAGATCCTGCGGCGAATCCCCGAACCTATCCTCTTGCCCGATGGCACCTGCATCACGGCATCGC
TTAGCATTGGTGTAGCCGTGTTTCCGATGCATGCGCAGGGCAGCCAGGGGTTGCTGCATGCGGCAGACGCGGCCATGTAC
GGCGCCAAGCGCCGATGCGGCGGGACCTGGCAGAGTGCCGAGCCACGCCTGGAGGCCAGCCGGGACCAGCGATGA

Upstream 100 bases:

>100_bases
ACCAGGTGTCGTTCCGCGTGAATCTCGATTCGCTGGCCCGCAGCGGTGTCCACGTCCATCCGGCCGTGCTGCAACTGGGT
CGCCGCAGGGGCGCGTCGTC

Downstream 100 bases:

>100_bases
CGCCTGCCTACCGTTCCGCTGCGGCAAGCCAGTTGCCGCATACCGGCCCGCGGGATACCCGCGGGCTGGCGGCGGGCGCA
CTGCTGGCGGCTCTGGCAGC

Product: GGDEF

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKPGSRPAGGRQRPTLYSTLRRIHLGVALLAVCTAGLSLTALGFAALRVYADHNLRLVARSISYTLEAPVVFRDKVATGE
TLAVIAASEDVSRATVLDLDNRVLADWQRPGHERRDGLRYALGELLSTGVIEQPILHDTQPVGTLRMTGDPSNLLQFLLQ
GALGLLACLLLTAVSAHYMSSRMLAQIVEPLRNLMRVAHAVRSERDFAQRTPPARIEELNALGDDFNGLLDELETWQNQM
QHENRSLAHRASHDSLTGLLNRSAFEELLELAVRAAAASASYAALLYLDSDDFKGINDRFGHAAGDVVLVALAQRIRASV
REGDVVARLGGDEFAVLVTSLRAAADIVPIAEQILRRIPEPILLPDGTCITASLSIGVAVFPMHAQGSQGLLHAADAAMY
GAKRRCGGTWQSAEPRLEASRDQR

Sequences:

>Translated_424_residues
MKPGSRPAGGRQRPTLYSTLRRIHLGVALLAVCTAGLSLTALGFAALRVYADHNLRLVARSISYTLEAPVVFRDKVATGE
TLAVIAASEDVSRATVLDLDNRVLADWQRPGHERRDGLRYALGELLSTGVIEQPILHDTQPVGTLRMTGDPSNLLQFLLQ
GALGLLACLLLTAVSAHYMSSRMLAQIVEPLRNLMRVAHAVRSERDFAQRTPPARIEELNALGDDFNGLLDELETWQNQM
QHENRSLAHRASHDSLTGLLNRSAFEELLELAVRAAAASASYAALLYLDSDDFKGINDRFGHAAGDVVLVALAQRIRASV
REGDVVARLGGDEFAVLVTSLRAAADIVPIAEQILRRIPEPILLPDGTCITASLSIGVAVFPMHAQGSQGLLHAADAAMY
GAKRRCGGTWQSAEPRLEASRDQR
>Mature_424_residues
MKPGSRPAGGRQRPTLYSTLRRIHLGVALLAVCTAGLSLTALGFAALRVYADHNLRLVARSISYTLEAPVVFRDKVATGE
TLAVIAASEDVSRATVLDLDNRVLADWQRPGHERRDGLRYALGELLSTGVIEQPILHDTQPVGTLRMTGDPSNLLQFLLQ
GALGLLACLLLTAVSAHYMSSRMLAQIVEPLRNLMRVAHAVRSERDFAQRTPPARIEELNALGDDFNGLLDELETWQNQM
QHENRSLAHRASHDSLTGLLNRSAFEELLELAVRAAAASASYAALLYLDSDDFKGINDRFGHAAGDVVLVALAQRIRASV
REGDVVARLGGDEFAVLVTSLRAAADIVPIAEQILRRIPEPILLPDGTCITASLSIGVAVFPMHAQGSQGLLHAADAAMY
GAKRRCGGTWQSAEPRLEASRDQR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=394, Percent_Identity=35.2791878172589, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87081977, Length=182, Percent_Identity=36.2637362637363, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=32.7777777777778, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787541, Length=167, Percent_Identity=35.3293413173653, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=37.7245508982036, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786584, Length=172, Percent_Identity=33.7209302325581, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=170, Percent_Identity=33.5294117647059, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=138, Percent_Identity=42.0289855072464, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081974, Length=109, Percent_Identity=37.6146788990826, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=32.7272727272727, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=34.3195266272189, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=94, Percent_Identity=37.2340425531915, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45947; Mature: 45947

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGSRPAGGRQRPTLYSTLRRIHLGVALLAVCTAGLSLTALGFAALRVYADHNLRLVAR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHH
SISYTLEAPVVFRDKVATGETLAVIAASEDVSRATVLDLDNRVLADWQRPGHERRDGLRY
HHHHHCCCCEEEECCCCCCCEEEEEEECCCCCHHHEEECCCHHHHHHCCCCHHHHHHHHH
ALGELLSTGVIEQPILHDTQPVGTLRMTGDPSNLLQFLLQGALGLLACLLLTAVSAHYMS
HHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMLAQIVEPLRNLMRVAHAVRSERDFAQRTPPARIEELNALGDDFNGLLDELETWQNQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
QHENRSLAHRASHDSLTGLLNRSAFEELLELAVRAAAASASYAALLYLDSDDFKGINDRF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHC
GHAAGDVVLVALAQRIRASVREGDVVARLGGDEFAVLVTSLRAAADIVPIAEQILRRIPE
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PILLPDGTCITASLSIGVAVFPMHAQGSQGLLHAADAAMYGAKRRCGGTWQSAEPRLEAS
CEECCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
RDQR
CCCC
>Mature Secondary Structure
MKPGSRPAGGRQRPTLYSTLRRIHLGVALLAVCTAGLSLTALGFAALRVYADHNLRLVAR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEHH
SISYTLEAPVVFRDKVATGETLAVIAASEDVSRATVLDLDNRVLADWQRPGHERRDGLRY
HHHHHCCCCEEEECCCCCCCEEEEEEECCCCCHHHEEECCCHHHHHHCCCCHHHHHHHHH
ALGELLSTGVIEQPILHDTQPVGTLRMTGDPSNLLQFLLQGALGLLACLLLTAVSAHYMS
HHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRMLAQIVEPLRNLMRVAHAVRSERDFAQRTPPARIEELNALGDDFNGLLDELETWQNQM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHH
QHENRSLAHRASHDSLTGLLNRSAFEELLELAVRAAAASASYAALLYLDSDDFKGINDRF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHC
GHAAGDVVLVALAQRIRASVREGDVVARLGGDEFAVLVTSLRAAADIVPIAEQILRRIPE
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PILLPDGTCITASLSIGVAVFPMHAQGSQGLLHAADAAMYGAKRRCGGTWQSAEPRLEAS
CEECCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCC
RDQR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]