Click here to switch to the map view.

The map label for this gene is glpK [H]

Identifier: 73541900

GI number: 73541900

Start: 2446046

End: 2447596

Strand: Direct

Name: glpK [H]

Synonym: Reut_A2213

Alternate gene names: 73541900

Gene position: 2446046-2447596 (Clockwise)

Preceding gene: 73541897

Following gene: 73541901

Centisome position: 64.26

GC content: 67.7

Gene sequence:

>1551_bases
ATGAACCAGCAGACCACGCAGGCCGCGCAGCAGCAGTTCGTACTGGCGCTGGACCAGGGCACGAGCAGTTCGCGGGCCAT
TCTGTTCGACCACGCCGGCAATGTGGTACGGCTTGCCCAGCGCGAATTCCGCCAATATTACCCACATCCCGGCCACGTCG
AGCACGACCCGTACGAAATCTGGCAGACGCAGCTCGCGGTCGCGCACGAGGTCCTGAACGACGCGGGTATCTCGGCCTCG
CAGTTGCGCGCAATCGGCATCACGAACCAACGCGAAACCACGGTCCTGTGGGACCGCAAGACCGGCGAGCCCGTGGGCCG
CGCGCTGGTCTGGCAAGACCGCCGCACCGCGCCCATGTGCGAGGCACTGCAGGCGGCCGGCCATGCCGGCATGATCCGCG
ACAAGACCGGGCTCGTCATCGATGCCTACTTCTCCGGCACCAAGCTGCGCTGGATGCTCGACAACGTCAAGGGCGCGCGC
GAACGCGCCCAGCGCGGCGAACTGGCGTTCGGCACGATCGACAGCTGGCTGATCTGGCAGCTCACCGACGGCGCGCGCCA
TGTGACCGACGTGTCCAACGCTTCGCGCACGATGCTGTTCAATATCCACAGCTTCGCATGGGACGATGCGCTACTCGCAT
TGCTGGACATCCCGCACAAGGTGCTGCCGGAGGTGGTGCCCTCCAGTGGCCAGGTCGCGCGCACGGTGCCGCGGCTGTTT
GGCGCGGAAGTCCCTATTGCCGGCATCGCGGGCGACCAGCAGGCCGCCACGTTCGGCCAGGCCTGCCTGTCTCCGGGCAT
GGCCAAGAACACGTATGGCACGGGTTGCTTCCTGCTGATGAATACGGGGCCGCAGCCGGTGGTCTCGCGCAACCGCTTGC
TGACGACGGTCGGATGGCAGATCAACGGGCAGACGCAATACTGCCTGGAAGGCGGTGTCTTTATGGGCGGCGCCACGATC
CAGTGGCTGCGCGATGGCCTGAAGATCATCGCAAGCGCCCCGGAGGTCGAGCCACTCGCGCGCCAGTGCACGGACACGGG
CGGTGTGGTGCTGGTGCCGGCCTTTGCCGGCCTCGGCGCGCCGCACTGGGATGCATTCGCACGCGGCACGCTGGTCGGTA
TGACGCGCGGCACCGGCCGCCCGCAGATCGCACGCGCGGCGCTGGAATCGATTGCGCTGCAGAGCGTGGACGTGCTCGAT
GCCATGCAGAAGGACGCCGGCATTTCGCTCGCGGAACTTCGCGTAGACGGCGGCGCGTCGCGCAGCGACCTGCTGATGCA
GATGCAGGCCGACCTGCTCGGCACGACCGTGGTACGTCCGCGCATCACCGAAACGACGGCACTGGGCGCAGCCTACCTGG
CCGGCCTCGCCACCGGGTACTGGAGCGACCAGGAGCAGATTTCCCGGCAATGGCAGGTGCAGCAGCGCTTCGAGCCCAAC
CTGTCCGCCGATGAACGCGCGCACCGGCTGTCGCGCTGGCACCGCGCCGTGGAACGGGCCCGCGACTGGGCCCGGGAAGA
TCACGAGGCCACCAGCGGCAAGAGAACGTGA

Upstream 100 bases:

>100_bases
GGGCTGCGCGCGGCGATGGACGCGTCAATGGCGGCAAGGGTGTCGGTGGGCATCGGCGGACGCAAGTTACGCAAGTTGAG
GATTTTCAGGGACGAAAGGC

Downstream 100 bases:

>100_bases
CGCGCCGTCGCGGCCACACCTTACGGAACCCTTTATTCAGATTCAGAGAGTGTCATGCCGAAAGCCCCGACCCCGATCCA
GCCGCCGTCCCGAGCCGCCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNQQTTQAAQQQFVLALDQGTSSSRAILFDHAGNVVRLAQREFRQYYPHPGHVEHDPYEIWQTQLAVAHEVLNDAGISAS
QLRAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEALQAAGHAGMIRDKTGLVIDAYFSGTKLRWMLDNVKGAR
ERAQRGELAFGTIDSWLIWQLTDGARHVTDVSNASRTMLFNIHSFAWDDALLALLDIPHKVLPEVVPSSGQVARTVPRLF
GAEVPIAGIAGDQQAATFGQACLSPGMAKNTYGTGCFLLMNTGPQPVVSRNRLLTTVGWQINGQTQYCLEGGVFMGGATI
QWLRDGLKIIASAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDAFARGTLVGMTRGTGRPQIARAALESIALQSVDVLD
AMQKDAGISLAELRVDGGASRSDLLMQMQADLLGTTVVRPRITETTALGAAYLAGLATGYWSDQEQISRQWQVQQRFEPN
LSADERAHRLSRWHRAVERARDWAREDHEATSGKRT

Sequences:

>Translated_516_residues
MNQQTTQAAQQQFVLALDQGTSSSRAILFDHAGNVVRLAQREFRQYYPHPGHVEHDPYEIWQTQLAVAHEVLNDAGISAS
QLRAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEALQAAGHAGMIRDKTGLVIDAYFSGTKLRWMLDNVKGAR
ERAQRGELAFGTIDSWLIWQLTDGARHVTDVSNASRTMLFNIHSFAWDDALLALLDIPHKVLPEVVPSSGQVARTVPRLF
GAEVPIAGIAGDQQAATFGQACLSPGMAKNTYGTGCFLLMNTGPQPVVSRNRLLTTVGWQINGQTQYCLEGGVFMGGATI
QWLRDGLKIIASAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDAFARGTLVGMTRGTGRPQIARAALESIALQSVDVLD
AMQKDAGISLAELRVDGGASRSDLLMQMQADLLGTTVVRPRITETTALGAAYLAGLATGYWSDQEQISRQWQVQQRFEPN
LSADERAHRLSRWHRAVERARDWAREDHEATSGKRT
>Mature_516_residues
MNQQTTQAAQQQFVLALDQGTSSSRAILFDHAGNVVRLAQREFRQYYPHPGHVEHDPYEIWQTQLAVAHEVLNDAGISAS
QLRAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEALQAAGHAGMIRDKTGLVIDAYFSGTKLRWMLDNVKGAR
ERAQRGELAFGTIDSWLIWQLTDGARHVTDVSNASRTMLFNIHSFAWDDALLALLDIPHKVLPEVVPSSGQVARTVPRLF
GAEVPIAGIAGDQQAATFGQACLSPGMAKNTYGTGCFLLMNTGPQPVVSRNRLLTTVGWQINGQTQYCLEGGVFMGGATI
QWLRDGLKIIASAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDAFARGTLVGMTRGTGRPQIARAALESIALQSVDVLD
AMQKDAGISLAELRVDGGASRSDLLMQMQADLLGTTVVRPRITETTALGAAYLAGLATGYWSDQEQISRQWQVQQRFEPN
LSADERAHRLSRWHRAVERARDWAREDHEATSGKRT

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI42794763, Length=519, Percent_Identity=43.9306358381503, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI4504007, Length=514, Percent_Identity=44.1634241245136, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI189409120, Length=514, Percent_Identity=44.1634241245136, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI41393575, Length=510, Percent_Identity=43.7254901960784, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=32.8767123287671, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1790361, Length=500, Percent_Identity=55.8, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1787789, Length=515, Percent_Identity=25.8252427184466, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789138, Length=496, Percent_Identity=22.5806451612903, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790005, Length=504, Percent_Identity=25.9920634920635, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789987, Length=484, Percent_Identity=25.8264462809917, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789168, Length=485, Percent_Identity=22.4742268041237, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=506, Percent_Identity=42.4901185770751, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=25.2895752895753, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=30.0675675675676, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321755, Length=559, Percent_Identity=36.6726296958855, Blast_Score=298, Evalue=2e-81,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=41.8467583497053, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24654573, Length=449, Percent_Identity=42.7616926503341, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24655555, Length=469, Percent_Identity=40.5117270788913, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655560, Length=469, Percent_Identity=40.5117270788913, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655570, Length=469, Percent_Identity=40.5117270788913, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655574, Length=469, Percent_Identity=40.5117270788913, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24655565, Length=469, Percent_Identity=40.5117270788913, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=30.8571428571429, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24652871, Length=507, Percent_Identity=31.163708086785, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=29.5719844357977, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=28.5714285714286, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24656372, Length=374, Percent_Identity=30.7486631016043, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI45552909, Length=315, Percent_Identity=26.984126984127, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 56491; Mature: 56491

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQTTQAAQQQFVLALDQGTSSSRAILFDHAGNVVRLAQREFRQYYPHPGHVEHDPYEI
CCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
WQTQLAVAHEVLNDAGISASQLRAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMC
HHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCCHHH
EALQAAGHAGMIRDKTGLVIDAYFSGTKLRWMLDNVKGARERAQRGELAFGTIDSWLIWQ
HHHHHCCCCCEEECCCCCEEEEEECCCEEEEEHHHHHHHHHHHHCCCEEEECCCCEEEEE
LTDGARHVTDVSNASRTMLFNIHSFAWDDALLALLDIPHKVLPEVVPSSGQVARTVPRLF
ECCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
GAEVPIAGIAGDQQAATFGQACLSPGMAKNTYGTGCFLLMNTGPQPVVSRNRLLTTVGWQ
CCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECEE
INGQTQYCLEGGVFMGGATIQWLRDGLKIIASAPEVEPLARQCTDTGGVVLVPAFAGLGA
ECCCEEEEECCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECHHCCCC
PHWDAFARGTLVGMTRGTGRPQIARAALESIALQSVDVLDAMQKDAGISLAELRVDGGAS
CCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
RSDLLMQMQADLLGTTVVRPRITETTALGAAYLAGLATGYWSDQEQISRQWQVQQRFEPN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
LSADERAHRLSRWHRAVERARDWAREDHEATSGKRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQQTTQAAQQQFVLALDQGTSSSRAILFDHAGNVVRLAQREFRQYYPHPGHVEHDPYEI
CCCHHHHHHHHHEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
WQTQLAVAHEVLNDAGISASQLRAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMC
HHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCHHCEEEECCCCCCHHH
EALQAAGHAGMIRDKTGLVIDAYFSGTKLRWMLDNVKGARERAQRGELAFGTIDSWLIWQ
HHHHHCCCCCEEECCCCCEEEEEECCCEEEEEHHHHHHHHHHHHCCCEEEECCCCEEEEE
LTDGARHVTDVSNASRTMLFNIHSFAWDDALLALLDIPHKVLPEVVPSSGQVARTVPRLF
ECCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHH
GAEVPIAGIAGDQQAATFGQACLSPGMAKNTYGTGCFLLMNTGPQPVVSRNRLLTTVGWQ
CCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECEE
INGQTQYCLEGGVFMGGATIQWLRDGLKIIASAPEVEPLARQCTDTGGVVLVPAFAGLGA
ECCCEEEEECCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEEECHHCCCC
PHWDAFARGTLVGMTRGTGRPQIARAALESIALQSVDVLDAMQKDAGISLAELRVDGGAS
CCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
RSDLLMQMQADLLGTTVVRPRITETTALGAAYLAGLATGYWSDQEQISRQWQVQQRFEPN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
LSADERAHRLSRWHRAVERARDWAREDHEATSGKRT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]