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The map label for this gene is gloB

Identifier: 73541875

GI number: 73541875

Start: 2415614

End: 2416414

Strand: Reverse

Name: gloB

Synonym: Reut_A2187

Alternate gene names: 73541875

Gene position: 2416414-2415614 (Counterclockwise)

Preceding gene: 73541886

Following gene: 73541874

Centisome position: 63.48

GC content: 67.17

Gene sequence:

>801_bases
ATGTTGAAAGTGGAGCCGATCCCCGCATTCCAGGATAACTATATCTGGGCCATCCACGACGGCCGCAATGCCGCCGTGGT
CGATCCCGGCGAGGCGGCGCCGGTGGAGCGGTTCCTGAAGGACAAGGGCCTGGCTCTGGGCGCTATTGTAATCACCCATC
ACCATGGTGATCACCAGGGCGGTGTGGCGGAGCTGCTTTCCGCCTATCCGACTACGCACGACGGCGCGCCGATGCCCGTG
ATCGGGCCCGCCGGGGAACGCATCGGCGGACGCACACAGGCCGTGCGCGAAGGCGATACGGTGACGTTGCCGGCGCCCGC
GCTGCAGTTTCGCGTGCTGGATGTGCCGGGTCATACGGCCGGCCATGTTGCTTACGCCGGCGACCTGCCAGGCGTTGGCC
CGGTCGTGTTCTGTGGTGATACGCTGTTCGCTACCGGCTGCGGCCGCCTGTTCGAGGGTACGCCGGCCCAGATGCTCGCG
TCGCTTGACAAGCTGGCGTCGCTGCCCGGTGAGACGCGCGTGTACTGCGCACATGAATACACGCGCAGCAATGTGCGCTT
CGCGCGCGCAGTGGAACCCGGCAACACCGCGCTGGCCGACTGGGAAGCGCGCGTCGAGTCGCTGCGTGAAGCGGGCCAGC
CGACTGTGCCGTCCACGATCGGACTCGAGCGCCAGGTCAATCCGTTCCTGCGTTCGCGAGAACCGACGGTGCGGGAGGCG
GTCAAGGCCCAGGGCGGCAGCGTGGCAGGAGATGCGGAAGCGTTCGGCGCGCTGCGTGGCTGGAAAGACAACTTCCGCTG
A

Upstream 100 bases:

>100_bases
CGTATTGCCGCGCCTCCCACTCGAGCATGTATTGCCCGGGGGGCGAAGCCAGCCAGTCTTCCCAGCTTACAATCGGACGG
TCAGCCATAGGAGCACGCGC

Downstream 100 bases:

>100_bases
GTGTAGTGATCAACCTCGCGGGCTCGCTTGCGTCCGTGCGGCAAGTGTCTGCGACAAGTCGAAAGCCAATTGTCTTCTGG
AAAATCTCATTGACGTGAAA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLKVEPIPAFQDNYIWAIHDGRNAAVVDPGEAAPVERFLKDKGLALGAIVITHHHGDHQGGVAELLSAYPTTHDGAPMPV
IGPAGERIGGRTQAVREGDTVTLPAPALQFRVLDVPGHTAGHVAYAGDLPGVGPVVFCGDTLFATGCGRLFEGTPAQMLA
SLDKLASLPGETRVYCAHEYTRSNVRFARAVEPGNTALADWEARVESLREAGQPTVPSTIGLERQVNPFLRSREPTVREA
VKAQGGSVAGDAEAFGALRGWKDNFR

Sequences:

>Translated_266_residues
MLKVEPIPAFQDNYIWAIHDGRNAAVVDPGEAAPVERFLKDKGLALGAIVITHHHGDHQGGVAELLSAYPTTHDGAPMPV
IGPAGERIGGRTQAVREGDTVTLPAPALQFRVLDVPGHTAGHVAYAGDLPGVGPVVFCGDTLFATGCGRLFEGTPAQMLA
SLDKLASLPGETRVYCAHEYTRSNVRFARAVEPGNTALADWEARVESLREAGQPTVPSTIGLERQVNPFLRSREPTVREA
VKAQGGSVAGDAEAFGALRGWKDNFR
>Mature_266_residues
MLKVEPIPAFQDNYIWAIHDGRNAAVVDPGEAAPVERFLKDKGLALGAIVITHHHGDHQGGVAELLSAYPTTHDGAPMPV
IGPAGERIGGRTQAVREGDTVTLPAPALQFRVLDVPGHTAGHVAYAGDLPGVGPVVFCGDTLFATGCGRLFEGTPAQMLA
SLDKLASLPGETRVYCAHEYTRSNVRFARAVEPGNTALADWEARVESLREAGQPTVPSTIGLERQVNPFLRSREPTVREA
VKAQGGSVAGDAEAFGALRGWKDNFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI94538320, Length=270, Percent_Identity=39.2592592592593, Blast_Score=175, Evalue=3e-44,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=39.2592592592593, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI14150041, Length=255, Percent_Identity=38.8235294117647, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI116642887, Length=276, Percent_Identity=36.9565217391304, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI21703352, Length=276, Percent_Identity=36.9565217391304, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI46361987, Length=199, Percent_Identity=38.6934673366834, Blast_Score=124, Evalue=7e-29,
Organism=Escherichia coli, GI1786406, Length=266, Percent_Identity=41.3533834586466, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17536925, Length=273, Percent_Identity=33.6996336996337, Blast_Score=140, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320478, Length=265, Percent_Identity=31.3207547169811, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324614, Length=249, Percent_Identity=30.5220883534137, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356335, Length=271, Percent_Identity=40.2214022140221, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24667711, Length=271, Percent_Identity=40.2214022140221, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24667703, Length=271, Percent_Identity=40.2214022140221, Blast_Score=184, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_CUPPJ (Q46Z82)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296395.1
- ProteinModelPortal:   Q46Z82
- SMR:   Q46Z82
- GeneID:   3609614
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2187
- NMPDR:   fig|264198.3.peg.2999
- HOGENOM:   HBG753931
- OMA:   FGHEYTL
- ProtClustDB:   CLSK896990
- BioCyc:   REUT264198:REUT_A2187-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28228; Mature: 28228

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVEPIPAFQDNYIWAIHDGRNAAVVDPGEAAPVERFLKDKGLALGAIVITHHHGDHQG
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCC
GVAELLSAYPTTHDGAPMPVIGPAGERIGGRTQAVREGDTVTLPAPALQFRVLDVPGHTA
CHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHCCCCEEEECCCCEEEEEEECCCCCC
GHVAYAGDLPGVGPVVFCGDTLFATGCGRLFEGTPAQMLASLDKLASLPGETRVYCAHEY
CCEEEECCCCCCCCEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHH
TRSNVRFARAVEPGNTALADWEARVESLREAGQPTVPSTIGLERQVNPFLRSREPTVREA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHCCCCHHHHH
VKAQGGSVAGDAEAFGALRGWKDNFR
HHHCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLKVEPIPAFQDNYIWAIHDGRNAAVVDPGEAAPVERFLKDKGLALGAIVITHHHGDHQG
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCC
GVAELLSAYPTTHDGAPMPVIGPAGERIGGRTQAVREGDTVTLPAPALQFRVLDVPGHTA
CHHHHHHHCCCCCCCCCCCEECCCCCCCCCCHHHHCCCCEEEECCCCEEEEEEECCCCCC
GHVAYAGDLPGVGPVVFCGDTLFATGCGRLFEGTPAQMLASLDKLASLPGETRVYCAHEY
CCEEEECCCCCCCCEEEECCHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEHHH
TRSNVRFARAVEPGNTALADWEARVESLREAGQPTVPSTIGLERQVNPFLRSREPTVREA
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHCCCCHHHHH
VKAQGGSVAGDAEAFGALRGWKDNFR
HHHCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA