Click here to switch to the map view.

The map label for this gene is mltD [H]

Identifier: 73541874

GI number: 73541874

Start: 2413787

End: 2415460

Strand: Reverse

Name: mltD [H]

Synonym: Reut_A2186

Alternate gene names: 73541874

Gene position: 2415460-2413787 (Counterclockwise)

Preceding gene: 73541875

Following gene: 73541873

Centisome position: 63.46

GC content: 67.03

Gene sequence:

>1674_bases
ATGAAATTTGGTCGATTACTGGCGGTGTTTGCAAGCGTGGCGCTGCTCGCAGCCTGCGCTAGCACGCCCACACCGCCCGG
CGCCAGTGCGTCCGGCGCCCCGACCGCCCTGACCTCCGCGCGCAGCGGCAAGGACCCGCTCAACTCGCTCAGCGTCGACA
AGACGCTTGCCCCTGGCGCAGCCAGCATGAACCCGGATCAGCCCGGGCTGGACTGGCTGCGTGGTCCGTCCACCGACATC
TGGGATCGCATCCGCCGTGGCTTCGCCATGCAGGACCTGGAAGGGACGCTGGTTGACGACCGCACGCAGTGGTATTCGCA
GCGACCGGAATACATGGAACGCATGGTGGCGCGTTCGAGCCGCTACCTGTATCACATCGTGGAAGAGCTGGAGCGGCGCA
ATATGCCGACCGAACTGGCGTTGCTGCCGTTCGTCGAGAGTGCGTTCAATCCGCAGGCGCAGTCGAGCGCCAAGGCGGCG
GGCATGTGGCAGTTCATCCCGAGCACGGGCAAGACCTATAACCTGAAGCAGAACATGTTCCGGGACGAGCGCCGCGATGT
GCTGGCGTCGACCGACGCGGCGCTCGACTACCTGGCGCGCCTCTATGACATGTTCGGCGACTGGCATCTCGCGCTGGCGG
CGTACAACTGGGGCGAGGGCGCGGTGTCGCGCGCGATTGCGCGCAACCAGGCGCGCGGCCTGCCGACGGACTACGCCAGC
CTGACCATGCCGAACGAGACACGCTACTACGTGCCCAAGCTGCAGGCGGTGAAGAACATCATCGCCAACCCGGCCGCGTA
CGGCGTCAGGCTGCCCGAGATCCCGGACCATCCGTATTTCGTGACGGTCACAACGAGCCGCGATATCGACGTCACGCTGG
CGGCCAAGCTGGCCAATATGTCGCTCGAGGAGTTCAAGGCGCTGAACCCGTCGTTCAACCGGCCGGTGATCCTGGGGGCG
TCGAATCCGCAGATCCTGCTGCCGTTCGATAACGCCGAACGCTTCCAGTACAACCTGAACACCTACCGCGGCGGGCTGTC
GAGCTGGACCGCGGTGACGGTGGACAACCGAGAACGCGTGGAAGCGCTCGCGGCGCGCCTCAATGTCGATCCGGACACGC
TGCGCGAGATCAACAGCATCCCACGCGGCATGCGGCTGAAGGCAGGTTCGACGGTGATGATCCCGCGCTCCGGCCACCAC
GACCAGGACATCAGCGCCACGCTCGCCGACAGTGCCATGCTGGCGATGGAGCCGGACCTGCCCGATGCCCGCCGCCTCGT
TGTGCGCGCAGGGCGCCGGGATACGGTGACCTCGGTGGCGCACCGCTACGGGGTATCGCCCAGCCAGGTGCAGTCGTGGA
ACAAGCTCTCAGGCACCAAGCTGGTCGCAGGGCAAAGCCTGGTGCTGATGGTGCCGGTTCGCGCCGGCGGCGCCGGCAAG
GCCACGCGCACCGCACGCGCGGAACCGGAGGCCGACAAGCCCGAGGTGGTACGCGTATCCGCGTCCGGCAAGGGCAAAGG
CAAGGCGGAAGGCCGCAAGCGCATGACGGTGGAAGCCGCACCACGCCAGGCGGCGAGCAAGAGCGCTGGCGTCTCGCGTT
CGGCCGCCGCCAAGTCTGCGCCGTCGGTCAAGGCCTCGTCAAAGGGGGCTTCGAAGGCTGCCAAGGCGCGATAG

Upstream 100 bases:

>100_bases
ACAAGTCGAAAGCCAATTGTCTTCTGGAAAATCTCATTGACGTGAAAGCCTGTTTTTTTTAGCATCACGCAACCTTTTAG
CGTCACAATTCGAGAATTTA

Downstream 100 bases:

>100_bases
GGCGCCGGAATCGCCGGACTAAACGGGGCGTTGCCTGGGCACGCCCCGTTTTTTTTGCCTCGGCGCGGCGCGGAGTGCCG
CATGCCCATCTGGCCGAAGG

Product: peptidoglycan-binding LysM:lytic transglycosylase, catalytic:MLTD_N

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKFGRLLAVFASVALLAACASTPTPPGASASGAPTALTSARSGKDPLNSLSVDKTLAPGAASMNPDQPGLDWLRGPSTDI
WDRIRRGFAMQDLEGTLVDDRTQWYSQRPEYMERMVARSSRYLYHIVEELERRNMPTELALLPFVESAFNPQAQSSAKAA
GMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLARLYDMFGDWHLALAAYNWGEGAVSRAIARNQARGLPTDYAS
LTMPNETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVTLAAKLANMSLEEFKALNPSFNRPVILGA
SNPQILLPFDNAERFQYNLNTYRGGLSSWTAVTVDNRERVEALAARLNVDPDTLREINSIPRGMRLKAGSTVMIPRSGHH
DQDISATLADSAMLAMEPDLPDARRLVVRAGRRDTVTSVAHRYGVSPSQVQSWNKLSGTKLVAGQSLVLMVPVRAGGAGK
ATRTARAEPEADKPEVVRVSASGKGKGKAEGRKRMTVEAAPRQAASKSAGVSRSAAAKSAPSVKASSKGASKAAKAR

Sequences:

>Translated_557_residues
MKFGRLLAVFASVALLAACASTPTPPGASASGAPTALTSARSGKDPLNSLSVDKTLAPGAASMNPDQPGLDWLRGPSTDI
WDRIRRGFAMQDLEGTLVDDRTQWYSQRPEYMERMVARSSRYLYHIVEELERRNMPTELALLPFVESAFNPQAQSSAKAA
GMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLARLYDMFGDWHLALAAYNWGEGAVSRAIARNQARGLPTDYAS
LTMPNETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVTLAAKLANMSLEEFKALNPSFNRPVILGA
SNPQILLPFDNAERFQYNLNTYRGGLSSWTAVTVDNRERVEALAARLNVDPDTLREINSIPRGMRLKAGSTVMIPRSGHH
DQDISATLADSAMLAMEPDLPDARRLVVRAGRRDTVTSVAHRYGVSPSQVQSWNKLSGTKLVAGQSLVLMVPVRAGGAGK
ATRTARAEPEADKPEVVRVSASGKGKGKAEGRKRMTVEAAPRQAASKSAGVSRSAAAKSAPSVKASSKGASKAAKAR
>Mature_557_residues
MKFGRLLAVFASVALLAACASTPTPPGASASGAPTALTSARSGKDPLNSLSVDKTLAPGAASMNPDQPGLDWLRGPSTDI
WDRIRRGFAMQDLEGTLVDDRTQWYSQRPEYMERMVARSSRYLYHIVEELERRNMPTELALLPFVESAFNPQAQSSAKAA
GMWQFIPSTGKTYNLKQNMFRDERRDVLASTDAALDYLARLYDMFGDWHLALAAYNWGEGAVSRAIARNQARGLPTDYAS
LTMPNETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVTLAAKLANMSLEEFKALNPSFNRPVILGA
SNPQILLPFDNAERFQYNLNTYRGGLSSWTAVTVDNRERVEALAARLNVDPDTLREINSIPRGMRLKAGSTVMIPRSGHH
DQDISATLADSAMLAMEPDLPDARRLVVRAGRRDTVTSVAHRYGVSPSQVQSWNKLSGTKLVAGQSLVLMVPVRAGGAGK
ATRTARAEPEADKPEVVRVSASGKGKGKAEGRKRMTVEAAPRQAASKSAGVSRSAAAKSAPSVKASSKGASKAAKAR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=409, Percent_Identity=36.6748166259169, Blast_Score=232, Evalue=4e-62,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 60462; Mature: 60462

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGRLLAVFASVALLAACASTPTPPGASASGAPTALTSARSGKDPLNSLSVDKTLAPGA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCC
ASMNPDQPGLDWLRGPSTDIWDRIRRGFAMQDLEGTLVDDRTQWYSQRPEYMERMVARSS
CCCCCCCCCCHHHCCCCHHHHHHHHCCCCHHCCCCCEECCHHHHHHCCHHHHHHHHHHHH
RYLYHIVEELERRNMPTELALLPFVESAFNPQAQSSAKAAGMWQFIPSTGKTYNLKQNMF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCHHHCCEEEECCCCCCEECHHHHHH
RDERRDVLASTDAALDYLARLYDMFGDWHLALAAYNWGEGAVSRAIARNQARGLPTDYAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCE
LTMPNETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVTLAAKLANM
EECCCCCEEECHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHC
SLEEFKALNPSFNRPVILGASNPQILLPFDNAERFQYNLNTYRGGLSSWTAVTVDNRERV
CHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHHH
EALAARLNVDPDTLREINSIPRGMRLKAGSTVMIPRSGHHDQDISATLADSAMLAMEPDL
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCEEEECCCCCCCCCCHHHHHCCEEEEECCCC
PDARRLVVRAGRRDTVTSVAHRYGVSPSQVQSWNKLSGTKLVAGQSLVLMVPVRAGGAGK
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCEEEEEEEEECCCCCC
ATRTARAEPEADKPEVVRVSASGKGKGKAEGRKRMTVEAAPRQAASKSAGVSRSAAAKSA
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHCCC
PSVKASSKGASKAAKAR
CCCCCCCCCCCHHHCCC
>Mature Secondary Structure
MKFGRLLAVFASVALLAACASTPTPPGASASGAPTALTSARSGKDPLNSLSVDKTLAPGA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCCCC
ASMNPDQPGLDWLRGPSTDIWDRIRRGFAMQDLEGTLVDDRTQWYSQRPEYMERMVARSS
CCCCCCCCCCHHHCCCCHHHHHHHHCCCCHHCCCCCEECCHHHHHHCCHHHHHHHHHHHH
RYLYHIVEELERRNMPTELALLPFVESAFNPQAQSSAKAAGMWQFIPSTGKTYNLKQNMF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCHHHCCEEEECCCCCCEECHHHHHH
RDERRDVLASTDAALDYLARLYDMFGDWHLALAAYNWGEGAVSRAIARNQARGLPTDYAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCE
LTMPNETRYYVPKLQAVKNIIANPAAYGVRLPEIPDHPYFVTVTTSRDIDVTLAAKLANM
EECCCCCEEECHHHHHHHHHHCCCHHCCCCCCCCCCCCEEEEEECCCCCCEEHHHHHHHC
SLEEFKALNPSFNRPVILGASNPQILLPFDNAERFQYNLNTYRGGLSSWTAVTVDNRERV
CHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCEEEEECHHHCCCCCCCEEEEECCHHHH
EALAARLNVDPDTLREINSIPRGMRLKAGSTVMIPRSGHHDQDISATLADSAMLAMEPDL
HHHHHHCCCCHHHHHHHHHCCCCCEECCCCEEEECCCCCCCCCCHHHHHCCEEEEECCCC
PDARRLVVRAGRRDTVTSVAHRYGVSPSQVQSWNKLSGTKLVAGQSLVLMVPVRAGGAGK
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCEEEEEEEEECCCCCC
ATRTARAEPEADKPEVVRVSASGKGKGKAEGRKRMTVEAAPRQAASKSAGVSRSAAAKSA
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCHHHHHCCC
PSVKASSKGASKAAKAR
CCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]