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The map label for this gene is bclA [H]

Identifier: 73541868

GI number: 73541868

Start: 2404553

End: 2406199

Strand: Reverse

Name: bclA [H]

Synonym: Reut_A2180

Alternate gene names: 73541868

Gene position: 2406199-2404553 (Counterclockwise)

Preceding gene: 73541869

Following gene: 73541867

Centisome position: 63.21

GC content: 67.4

Gene sequence:

>1647_bases
ATGGCGACCACCGCCCACCTCGATACCTTCGCCCAGGATCGGCTGCCGCCGCCCGAGGCGTGGCCCGAGTTCCGCTTCAA
TGCGGATACCGACTACCCCGAGCGCCTGAACTGCGCCGTGGAACTGGTTGACCGGCACGTGCGCGAAGGGCGCGGCGAGC
GCGTGGCCATCCGCCAGCGGCGCGGCGACGGCATCGAGACGATCACGTTCGCGCAGCTCGCGGCGCTGAGCAACCGCATT
GCCCATGTGCTCACCGAGGATATGAAGCTGGTGCCGGGCAATCGCGTGCTGCTGCGCGGGCCCAATAACCTGATGATGGC
GGCGAGCTGGCTAGCGACCATCAAGGCGGGCCTGATCGCGGTGCCGACCATGCCGTTGCTGCGCGCCAAGGAGCTCAAGC
AGATCATCGACAAGGCGCAGGTCACTGCGGCGCTGTGCGACGTGCGCCTGCGCGAGGAGCTGGAGGCCAACCGGCAGCCG
GGCGCGGAATATCACTGCCCGAGCCTGGCCCAGGCGCTGTACTTCAACGGCGAAGGCGAAGGCTCGCTGGAAGCCGCCAT
GGCGGGCAAGCCGGACACCTTCGATGCCTGCGATACGGCGACCGACGACATCTGCCTGATCGCCTTCACCAGCGGCACCA
CGGGCCAGCCCAAGGGCACGGTCCACTTCCATCGCGATGTGCTTGCGATGTGCGACTTATTCCCGCGCCATGTGCTGCGC
CCGGAACCGGACGACATCTTCTGCGGCACGCCGCCGATCGCCTTCACGTTCGGGCTAGGCGGCATCCTGTGCTTCCCATG
GCGCATCGGCGCCAGCACCGTGCTGGCGGAGAAGCTCACGCCGGACAGCCTGCTGCAGCTGATCGCGGACTTCCGCGCAA
CCATCGTGTGCACCGCGCCGACCTTCTACCGTCAGATGGCCGCGCTGGCGTCGCGTTACGACTTGCGCAGTCTGAAGAAA
AGCGTGTCGGCCGGCGAGGCGCTGCCCGATGCCACGCGCCAGGCGTGGAAGGCGGCTACCGGCATTGAGATGACGGACGG
CATCGGTGGCACGGAGATGATGCACATCTTCATCTCCAGCGCGGGTGCCGATGTGAAGCCGGGTTCCATCGGCAAGGTCG
TGCCCGGCTACATCGCGCAGATCGTCGACGAGAACATGCAGCCCGTGCCGCCCGGCACCGTGGGCAAGCTGGCGGTGCGC
GGGCCGACCGGCTGCCGCTACCTGGACGACCCCCGTCAGGCCAACTACGTCAAGGCTGGATGGAACCTGCCCGGCGACAC
CTTCGTCATGGACAAGGATGGTTACTACTTCTACCAGGCGCGCTCCGACGACATGATCATCTCGGCCGGCTACAACATCG
CCGGTCCGGAGGTGGAGAGCACGCTGATGCAGCACAAGGACGTGGCGGAATGCGGCGTGGTCGGCGCGCCGGATGCGGAG
CGTGGGCAGGTCGTCACGGCCTACGTCGTGCTGCGCGAAGGGGTGGAGGCCAGCGACGCCACGCGCGCCGCGCTGCAGGA
CTATGTGAAGCGCGAGATCGCGCCATACAAGTATCCGCGGCGCATCGTCTTCGTGCCGGCACTGCCACGCACGGAGACTG
GCAAGCTGCAGCGGTTCCGGCTGCGCCAGATGGCGGAGCAGCCATGA

Upstream 100 bases:

>100_bases
CTCATCATCGCGCGCGAAACGCTGGCGGCGCAGGTCGCCTGAAGCGGCACGGCCCGACACACATCATCAAGTCAGCGACA
CAGCAGACAGGAGACAAGTC

Downstream 100 bases:

>100_bases
CTGCCGTATTCCGCAATACCGTGCTGGTGCGCTTCAAGCACTGTGACGCCGCCGGCATCGTGTTCTATCCGCGCTACTTC
GAGATGCTCAACGACTTCAT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MATTAHLDTFAQDRLPPPEAWPEFRFNADTDYPERLNCAVELVDRHVREGRGERVAIRQRRGDGIETITFAQLAALSNRI
AHVLTEDMKLVPGNRVLLRGPNNLMMAASWLATIKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDVRLREELEANRQP
GAEYHCPSLAQALYFNGEGEGSLEAAMAGKPDTFDACDTATDDICLIAFTSGTTGQPKGTVHFHRDVLAMCDLFPRHVLR
PEPDDIFCGTPPIAFTFGLGGILCFPWRIGASTVLAEKLTPDSLLQLIADFRATIVCTAPTFYRQMAALASRYDLRSLKK
SVSAGEALPDATRQAWKAATGIEMTDGIGGTEMMHIFISSAGADVKPGSIGKVVPGYIAQIVDENMQPVPPGTVGKLAVR
GPTGCRYLDDPRQANYVKAGWNLPGDTFVMDKDGYYFYQARSDDMIISAGYNIAGPEVESTLMQHKDVAECGVVGAPDAE
RGQVVTAYVVLREGVEASDATRAALQDYVKREIAPYKYPRRIVFVPALPRTETGKLQRFRLRQMAEQP

Sequences:

>Translated_548_residues
MATTAHLDTFAQDRLPPPEAWPEFRFNADTDYPERLNCAVELVDRHVREGRGERVAIRQRRGDGIETITFAQLAALSNRI
AHVLTEDMKLVPGNRVLLRGPNNLMMAASWLATIKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDVRLREELEANRQP
GAEYHCPSLAQALYFNGEGEGSLEAAMAGKPDTFDACDTATDDICLIAFTSGTTGQPKGTVHFHRDVLAMCDLFPRHVLR
PEPDDIFCGTPPIAFTFGLGGILCFPWRIGASTVLAEKLTPDSLLQLIADFRATIVCTAPTFYRQMAALASRYDLRSLKK
SVSAGEALPDATRQAWKAATGIEMTDGIGGTEMMHIFISSAGADVKPGSIGKVVPGYIAQIVDENMQPVPPGTVGKLAVR
GPTGCRYLDDPRQANYVKAGWNLPGDTFVMDKDGYYFYQARSDDMIISAGYNIAGPEVESTLMQHKDVAECGVVGAPDAE
RGQVVTAYVVLREGVEASDATRAALQDYVKREIAPYKYPRRIVFVPALPRTETGKLQRFRLRQMAEQP
>Mature_547_residues
ATTAHLDTFAQDRLPPPEAWPEFRFNADTDYPERLNCAVELVDRHVREGRGERVAIRQRRGDGIETITFAQLAALSNRIA
HVLTEDMKLVPGNRVLLRGPNNLMMAASWLATIKAGLIAVPTMPLLRAKELKQIIDKAQVTAALCDVRLREELEANRQPG
AEYHCPSLAQALYFNGEGEGSLEAAMAGKPDTFDACDTATDDICLIAFTSGTTGQPKGTVHFHRDVLAMCDLFPRHVLRP
EPDDIFCGTPPIAFTFGLGGILCFPWRIGASTVLAEKLTPDSLLQLIADFRATIVCTAPTFYRQMAALASRYDLRSLKKS
VSAGEALPDATRQAWKAATGIEMTDGIGGTEMMHIFISSAGADVKPGSIGKVVPGYIAQIVDENMQPVPPGTVGKLAVRG
PTGCRYLDDPRQANYVKAGWNLPGDTFVMDKDGYYFYQARSDDMIISAGYNIAGPEVESTLMQHKDVAECGVVGAPDAER
GQVVTAYVVLREGVEASDATRAALQDYVKREIAPYKYPRRIVFVPALPRTETGKLQRFRLRQMAEQP

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=536, Percent_Identity=32.8358208955224, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI122937307, Length=533, Percent_Identity=33.2082551594747, Blast_Score=241, Evalue=2e-63,
Organism=Homo sapiens, GI38505220, Length=535, Percent_Identity=33.4579439252336, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI58082049, Length=532, Percent_Identity=30.2631578947368, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=30.0751879699248, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=30.0751879699248, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI115511026, Length=546, Percent_Identity=30.2197802197802, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=27.3921200750469, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=27.3921200750469, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI13375727, Length=552, Percent_Identity=26.9927536231884, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI156151445, Length=524, Percent_Identity=27.6717557251908, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI28416953, Length=552, Percent_Identity=26.268115942029, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI42544134, Length=365, Percent_Identity=28.7671232876712, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195947378, Length=445, Percent_Identity=26.9662921348315, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI8923896, Length=576, Percent_Identity=24.6527777777778, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI253970489, Length=384, Percent_Identity=28.3854166666667, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI145693145, Length=468, Percent_Identity=28.8461538461538, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1788107, Length=513, Percent_Identity=25.7309941520468, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1790505, Length=547, Percent_Identity=27.4223034734918, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI221142682, Length=503, Percent_Identity=25.2485089463221, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=26, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786529, Length=379, Percent_Identity=28.7598944591029, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=23.3396584440228, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786801, Length=370, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=516, Percent_Identity=27.1317829457364, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=516, Percent_Identity=27.1317829457364, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI71994690, Length=516, Percent_Identity=27.1317829457364, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=547, Percent_Identity=25.0457038391225, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=23.6514522821577, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=500, Percent_Identity=26.2, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=26.0989010989011, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=369, Percent_Identity=25.7452574525745, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=390, Percent_Identity=26.1538461538462, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=488, Percent_Identity=23.155737704918, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=349, Percent_Identity=27.5071633237822, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71982997, Length=366, Percent_Identity=27.0491803278689, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=366, Percent_Identity=27.0491803278689, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=357, Percent_Identity=25.2100840336134, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=569, Percent_Identity=27.0650263620387, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6319264, Length=588, Percent_Identity=26.1904761904762, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319699, Length=352, Percent_Identity=26.9886363636364, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24581924, Length=490, Percent_Identity=27.5510204081633, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24667955, Length=568, Percent_Identity=26.2323943661972, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI62472339, Length=379, Percent_Identity=27.9683377308707, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=27.9683377308707, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=24.1584158415842, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355181, Length=365, Percent_Identity=25.4794520547945, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI18859661, Length=361, Percent_Identity=25.4847645429363, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21355225, Length=558, Percent_Identity=21.8637992831541, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24656500, Length=509, Percent_Identity=22.7897838899804, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=356, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648257, Length=348, Percent_Identity=25.8620689655172, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130357, Length=356, Percent_Identity=23.0337078651685, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356441, Length=355, Percent_Identity=25.3521126760563, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653035, Length=495, Percent_Identity=23.2323232323232, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 59975; Mature: 59844

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTAHLDTFAQDRLPPPEAWPEFRFNADTDYPERLNCAVELVDRHVREGRGERVAIRQR
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RGDGIETITFAQLAALSNRIAHVLTEDMKLVPGNRVLLRGPNNLMMAASWLATIKAGLIA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHHHHCEEE
VPTMPLLRAKELKQIIDKAQVTAALCDVRLREELEANRQPGAEYHCPSLAQALYFNGEGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEECCCCC
GSLEAAMAGKPDTFDACDTATDDICLIAFTSGTTGQPKGTVHFHRDVLAMCDLFPRHVLR
CCCHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCC
PEPDDIFCGTPPIAFTFGLGGILCFPWRIGASTVLAEKLTPDSLLQLIADFRATIVCTAP
CCCCCEECCCCCCEEHHCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCEEEEEECH
TFYRQMAALASRYDLRSLKKSVSAGEALPDATRQAWKAATGIEMTDGIGGTEMMHIFISS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHEEEEEEEC
AGADVKPGSIGKVVPGYIAQIVDENMQPVPPGTVGKLAVRGPTGCRYLDDPRQANYVKAG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
WNLPGDTFVMDKDGYYFYQARSDDMIISAGYNIAGPEVESTLMQHKDVAECGVVGAPDAE
CCCCCCEEEEECCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCHHHHCCCCCCCCC
RGQVVTAYVVLREGVEASDATRAALQDYVKREIAPYKYPRRIVFVPALPRTETGKLQRFR
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHH
LRQMAEQP
HHHHHCCC
>Mature Secondary Structure 
ATTAHLDTFAQDRLPPPEAWPEFRFNADTDYPERLNCAVELVDRHVREGRGERVAIRQR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEC
RGDGIETITFAQLAALSNRIAHVLTEDMKLVPGNRVLLRGPNNLMMAASWLATIKAGLIA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCCHHHHHHHHHHHHHCEEE
VPTMPLLRAKELKQIIDKAQVTAALCDVRLREELEANRQPGAEYHCPSLAQALYFNGEGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEECCCCC
GSLEAAMAGKPDTFDACDTATDDICLIAFTSGTTGQPKGTVHFHRDVLAMCDLFPRHVLR
CCCHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHHCC
PEPDDIFCGTPPIAFTFGLGGILCFPWRIGASTVLAEKLTPDSLLQLIADFRATIVCTAP
CCCCCEECCCCCCEEHHCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHCCEEEEEECH
TFYRQMAALASRYDLRSLKKSVSAGEALPDATRQAWKAATGIEMTDGIGGTEMMHIFISS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHEEEEEEEC
AGADVKPGSIGKVVPGYIAQIVDENMQPVPPGTVGKLAVRGPTGCRYLDDPRQANYVKAG
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEC
WNLPGDTFVMDKDGYYFYQARSDDMIISAGYNIAGPEVESTLMQHKDVAECGVVGAPDAE
CCCCCCEEEEECCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHCCHHHHCCCCCCCCC
RGQVVTAYVVLREGVEASDATRAALQDYVKREIAPYKYPRRIVFVPALPRTETGKLQRFR
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHH
LRQMAEQP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]