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The map label for this gene is carA [H]

Identifier: 73541865

GI number: 73541865

Start: 2402183

End: 2403319

Strand: Reverse

Name: carA [H]

Synonym: Reut_A2177

Alternate gene names: 73541865

Gene position: 2403319-2402183 (Counterclockwise)

Preceding gene: 73541866

Following gene: 73541864

Centisome position: 63.14

GC content: 62.97

Gene sequence:

>1137_bases
GTGTTACCGTCTTTCCCGTCCGCCATTCTCGCGCTTGCAGACGGCACGGTCTTTCGTGGCTATTCCATCGGCGCTTCCGG
CCATACCATCGGCGAAGTGGTGTTCAACACCGCCATCACCGGTTATCAGGAAATCCTCACCGACCCGAGCTATTCCCGGC
AGATCGTCACGCTGACGTATCCGCACATCGGGAATTACGGTGTCAACCGTGAGGATGTTGAAGCCACGAAAGTCCATGCC
GCCGGCCTGATCATCAAGGATCTGCCGATTCTGGCCTCGAATTTCCGCAAGGAGCATACGCTCGGCCATTACCTGAAGCA
GGAAAAGGTCGTCGCCATTGCCGGCATCGATACCCGCAAGCTGACCCGCATCCTGCGCGAGAAGGGCGCCCAGAACGGCT
GCATCCTGGCTGGCGAGGACAACGTCCAGAAGGCCATCGACCTGGCGCGCTCGTTCCCCGGCCTGGCTGGCATGGACCTG
GCCAAGGTGGTGTCGGTCAAGGAGCCGTATGACTGGACGCAGTCGGAGTGGGAACTCGGCCAGGGCTACGGCAAGCAGGA
CAAGCCGCAGTTCCACGTGGTCGCCTATGACTACGGCGTCAAGTTCAATATCCTGCGCATGCTGGCCGAGCGCGGCTGCA
AGGTGACGGTGCTGCCGGCGCAGGCCACTGCGGCCGACGCCCTGGCGCTGAATCCGGATGGCGTGTTCCTGTCCAACGGC
CCCGGCGATCCGGAACCGTGCGACTACGCGATCAAGGCCACGCGCGAATTCATCGAGCGCGGCATCCCGACCTTCGGCAT
CTGCCTGGGCCACCAGATCATGGCGCTGGCCATCGGTGCCAAGACGCTGAAGATGAAGGCCGGCCACCACGGCGCCAACC
ATCCGGTCAAGGATCTCGATGACGGTCGCGTGATGATCACGTCGCAGAACCACGGCTTTGCTGTGGATGCGGAAACGCTG
CCGGCCAATGCGCGCGCGACGCACGTGTCTCTGTTCGATGGCACGCTGCAGGGCTTTGCGCTGACCGACAAGCCGGCATT
CTGCTTCCAGGGCCACCCGGAAGCGTCGCCTGGCCCGCACGACGTGGCTTACCTGTTCGACCGCTTCACGAAGATGATGG
CGGACGCGCGCAAGTAA

Upstream 100 bases:

>100_bases
CGCCGCTTGGCACAGTGCCAATGCAGCCGTACCGGGATCTCAATGGGGCACGAGGCGTTGATCAGGTCGGTCACGGGGTG
AGTCCAGCAGGAGCCTACCC

Downstream 100 bases:

>100_bases
GCGCGGCCCATCACGAGCAGCGAAGAACAGAGACGAGCAGGGAACAAGGACACGCCATGAACGCCTTCATGCATACCGCC
TTCGGCATCACCGATTTCTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLGHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYDWTQSEWELGQGYGKQDKPQFHVVAYDYGVKFNILRMLAERGCKVTVLPAQATAADALALNPDGVFLSNG
PGDPEPCDYAIKATREFIERGIPTFGICLGHQIMALAIGAKTLKMKAGHHGANHPVKDLDDGRVMITSQNHGFAVDAETL
PANARATHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDVAYLFDRFTKMMADARK

Sequences:

>Translated_378_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLGHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYDWTQSEWELGQGYGKQDKPQFHVVAYDYGVKFNILRMLAERGCKVTVLPAQATAADALALNPDGVFLSNG
PGDPEPCDYAIKATREFIERGIPTFGICLGHQIMALAIGAKTLKMKAGHHGANHPVKDLDDGRVMITSQNHGFAVDAETL
PANARATHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDVAYLFDRFTKMMADARK
>Mature_378_residues
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNREDVEATKVHA
AGLIIKDLPILASNFRKEHTLGHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDL
AKVVSVKEPYDWTQSEWELGQGYGKQDKPQFHVVAYDYGVKFNILRMLAERGCKVTVLPAQATAADALALNPDGVFLSNG
PGDPEPCDYAIKATREFIERGIPTFGICLGHQIMALAIGAKTLKMKAGHHGANHPVKDLDDGRVMITSQNHGFAVDAETL
PANARATHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDVAYLFDRFTKMMADARK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=38.4816753926702, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI21361331, Length=383, Percent_Identity=36.0313315926893, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI169790915, Length=383, Percent_Identity=36.0313315926893, Blast_Score=218, Evalue=6e-57,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=66.2234042553192, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1789760, Length=128, Percent_Identity=30.46875, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=386, Percent_Identity=38.860103626943, Blast_Score=251, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=36.734693877551, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=38.4615384615385, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24642586, Length=381, Percent_Identity=43.0446194225722, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=43.0446194225722, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40975; Mature: 40975

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCHHHHHCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNYGVNREDVEATKVHAAGLIIKDLPILASNFRKEHTLGHYLKQEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDLAKVVSVKEPYDWTQSEWELG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHH
QGYGKQDKPQFHVVAYDYGVKFNILRMLAERGCKVTVLPAQATAADALALNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCC
PGDPEPCDYAIKATREFIERGIPTFGICLGHQIMALAIGAKTLKMKAGHHGANHPVKDLD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
DGRVMITSQNHGFAVDAETLPANARATHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCEEECCCCCCCCCCH
DVAYLFDRFTKMMADARK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTY
CCCCCCCHHHHHCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
PHIGNYGVNREDVEATKVHAAGLIIKDLPILASNFRKEHTLGHYLKQEKVVAIAGIDTRK
CCCCCCCCCCCCCHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHH
LTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDLAKVVSVKEPYDWTQSEWELG
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCHHHHH
QGYGKQDKPQFHVVAYDYGVKFNILRMLAERGCKVTVLPAQATAADALALNPDGVFLSNG
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEECCC
PGDPEPCDYAIKATREFIERGIPTFGICLGHQIMALAIGAKTLKMKAGHHGANHPVKDLD
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
DGRVMITSQNHGFAVDAETLPANARATHVSLFDGTLQGFALTDKPAFCFQGHPEASPGPH
CCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCCEEECCCCCEEECCCCCCCCCCH
DVAYLFDRFTKMMADARK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]