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The map label for this gene is yeaS [C]

Identifier: 73541864

GI number: 73541864

Start: 2401443

End: 2402126

Strand: Reverse

Name: yeaS [C]

Synonym: Reut_A2176

Alternate gene names: 73541864

Gene position: 2402126-2401443 (Counterclockwise)

Preceding gene: 73541865

Following gene: 73541863

Centisome position: 63.11

GC content: 65.64

Gene sequence:

>684_bases
ATGAACGCCTTCATGCATACCGCCTTCGGCATCACCGATTTCTGGACCTATGTGCTGGGCACGATCTTCATCGTGCTGCT
GCCGGGGCCGAACTCGATGTATGTGCTGTCCGTGGCGGCGCAGCGCGGTGTTCGCGCGGGCTACAAGGGTGCGTGCGGCG
TTTTCCTTGGCGATGCGGTGCTGATGGTGCTGTCGGCCGCGGGCGTGGCCTCGCTGCTCAAGGCAAGCCCCGCACTGTTC
TACGTGGTGAAGTACATCGGCGCGGCCTACCTCGGCTGGATCGGGTTCAACATGCTGCGCGGCGCGGTCCGTAACTGGAC
GAGCGCCGGCAAGACGGAGCGGGCTGCGGCGGAAGCCGCCGTTGCCGCGCCGGCGGACCAGTCCAACGCGTTCCGCAAGG
CGCTGGTCATCAGCCTGATGAACCCCAAGGCCATCCTGTTCTTCATCTCGTTCTTCATCCAGTTCGTCGATCCGGGCTTC
GGCTATGCGGCGCTGTCGTTCCTGGCGCTGGGCGTGGTCGTGCAGATATTCAGCTTCCTGTACCTGACGACGCTGATCTT
CGTCGGTGTGAAGCTGGCCGCCGCGTTCCGCCGCCGCCGACGCCTGTCGGCAGGCGTGTCGAGCGGCGTGGGCGCCATGT
TCATCGGCTTCGGCGCGAAGCTGGCGACGGCCACCCTGAGCTGA

Upstream 100 bases:

>100_bases
TGTTCGACCGCTTCACGAAGATGATGGCGGACGCGCGCAAGTAAGCGCGGCCCATCACGAGCAGCGAAGAACAGAGACGA
GCAGGGAACAAGGACACGCC

Downstream 100 bases:

>100_bases
ACTTTCCGAATCCTGCTGGATTCCTGATTTTCCGGACGGCGGCCCCGATGGCCGCCGGACCAAAAAAGAGATAGTGCAAT
GCCAAAACGCACAGACATCA

Product: leucine export protein LeuE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNAFMHTAFGITDFWTYVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAVLMVLSAAGVASLLKASPALF
YVVKYIGAAYLGWIGFNMLRGAVRNWTSAGKTERAAAEAAVAAPADQSNAFRKALVISLMNPKAILFFISFFIQFVDPGF
GYAALSFLALGVVVQIFSFLYLTTLIFVGVKLAAAFRRRRRLSAGVSSGVGAMFIGFGAKLATATLS

Sequences:

>Translated_227_residues
MNAFMHTAFGITDFWTYVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAVLMVLSAAGVASLLKASPALF
YVVKYIGAAYLGWIGFNMLRGAVRNWTSAGKTERAAAEAAVAAPADQSNAFRKALVISLMNPKAILFFISFFIQFVDPGF
GYAALSFLALGVVVQIFSFLYLTTLIFVGVKLAAAFRRRRRLSAGVSSGVGAMFIGFGAKLATATLS
>Mature_227_residues
MNAFMHTAFGITDFWTYVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAVLMVLSAAGVASLLKASPALF
YVVKYIGAAYLGWIGFNMLRGAVRNWTSAGKTERAAAEAAVAAPADQSNAFRKALVISLMNPKAILFFISFFIQFVDPGF
GYAALSFLALGVVVQIFSFLYLTTLIFVGVKLAAAFRRRRRLSAGVSSGVGAMFIGFGAKLATATLS

Specific function: Unknown

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI1788099, Length=219, Percent_Identity=42.0091324200913, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI48994979, Length=213, Percent_Identity=25.8215962441315, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI48994978, Length=208, Percent_Identity=28.3653846153846, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786522, Length=125, Percent_Identity=31.2, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 24072; Mature: 24072

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFMHTAFGITDFWTYVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAV
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHH
LMVLSAAGVASLLKASPALFYVVKYIGAAYLGWIGFNMLRGAVRNWTSAGKTERAAAEAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VAAPADQSNAFRKALVISLMNPKAILFFISFFIQFVDPGFGYAALSFLALGVVVQIFSFL
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLTTLIFVGVKLAAAFRRRRRLSAGVSSGVGAMFIGFGAKLATATLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAFMHTAFGITDFWTYVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAV
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHH
LMVLSAAGVASLLKASPALFYVVKYIGAAYLGWIGFNMLRGAVRNWTSAGKTERAAAEAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VAAPADQSNAFRKALVISLMNPKAILFFISFFIQFVDPGFGYAALSFLALGVVVQIFSFL
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YLTTLIFVGVKLAAAFRRRRRLSAGVSSGVGAMFIGFGAKLATATLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]