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The map label for this gene is phoB [H]

Identifier: 73541850

GI number: 73541850

Start: 2385446

End: 2386153

Strand: Reverse

Name: phoB [H]

Synonym: Reut_A2162

Alternate gene names: 73541850

Gene position: 2386153-2385446 (Counterclockwise)

Preceding gene: 73541851

Following gene: 73541849

Centisome position: 62.69

GC content: 61.86

Gene sequence:

>708_bases
ATGCCAAGCAGTATTCTCGTTGTCGAAGATGAACCGGCGATCGCTGAACTAATCGCCGTCAACCTGCAGCACGCGGGGCA
CTATCCGATCCGGGCCTACAACGCCGAACAGGCGCTGTCGCTGATGAGCGACGTGCTGCCAGACCTGGTGCTGCTGGACT
GGATGCTACCGGGCAAGTCAGGCGCCACCTTCGCCAAGGAGTTGCGTGCGAACGACCGCACCAAGCAGATTCCCATCATC
ATGCTCACCGCCCGCAGCGAAGAGCAGGACAAGGTGATGGGGCTCGAAGCCGGTGCGGACGACTATGTCACCAAGCCGTT
TTCGCCCAAGGAACTGCTTGCCCGCATCAAAGCCGTGCTGCGCCGCCGTGCGCCGCAGCTCACCGATGATGTGGTGGCCA
TCAATGGCCTGCGTCTGGATCCGGCCACGCACCGCGTGACCGGCGAAGACAACGCCGGCCCGATCAAGCTCGACCTCGGT
CCTACAGAGTTCCGGTTGCTTCACTTCCTGATGACGCATCCGGAGCGCGTGCATAGCCGTTCGCAACTGCTGGATCAAGT
GTGGGGCGATCACGTGTTCGTCGAAGAACGTACGGTCGACGTGCATATCAAGCGCCTGCGTGCTGCGCTGACGCCGGGCG
GCTACAGCAATATGATTGAAACCGTACGCGGCAGCGGTTACCGGCTGGCTCGCACGCCAGGCAATTGA

Upstream 100 bases:

>100_bases
ATCTACATCGTCAAGGGGACGGATGTCCGCCACGCCTCGCGCGAGGACATGGAGCGTGAGGCCCTGAGCTGAGCGGCGTT
ATCTCCGGAGAAACATACAC

Downstream 100 bases:

>100_bases
GATTGGCCCTGGCCATCCTTTGCGTGGCCAGTTGTCACGGATTCGTGGCAAACTCTGCCGCAACGGCGGTTCCCCCGCCA
CTTGCCCGCACCGCGGGTTC

Product: phosphate regulon transcriptional regulatory protein phoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRANDRTKQIPII
MLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGEDNAGPIKLDLG
PTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARTPGN

Sequences:

>Translated_235_residues
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRANDRTKQIPII
MLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGEDNAGPIKLDLG
PTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARTPGN
>Mature_234_residues
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRANDRTKQIPIIM
LTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGEDNAGPIKLDLGP
TEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARTPGN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=56.9565217391304, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=42.0353982300885, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1789809, Length=231, Percent_Identity=38.5281385281385, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=39.0557939914163, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=36.7713004484305, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=35.8407079646018, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.0807174887892, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=33.8983050847458, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=35.2173913043478, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=38.034188034188, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.7552742616034, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1789402, Length=228, Percent_Identity=33.7719298245614, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790552, Length=232, Percent_Identity=32.7586206896552, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=32.9166666666667, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26125; Mature: 25994

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GATFAKELRANDRTKQIPIIMLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVL
CCHHHHHHHCCCCCCCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLRLDPATHRVTGEDNAGPIKLDLGPTEFRLLHFLMTHPERVHSR
HHCCCCCCCCEEEECCEEECCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
SQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARTPGN
HHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure 
PSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKS
CCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCC
GATFAKELRANDRTKQIPIIMLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVL
CCHHHHHHHCCCCCCCCCEEEEECCCCCCHHEEEECCCCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTDDVVAINGLRLDPATHRVTGEDNAGPIKLDLGPTEFRLLHFLMTHPERVHSR
HHCCCCCCCCEEEECCEEECCCCCEECCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHH
SQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARTPGN
HHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]