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The map label for this gene is pcm

Identifier: 73541785

GI number: 73541785

Start: 2305032

End: 2305925

Strand: Reverse

Name: pcm

Synonym: Reut_A2097

Alternate gene names: 73541785

Gene position: 2305925-2305032 (Counterclockwise)

Preceding gene: 73541786

Following gene: 73541784

Centisome position: 60.58

GC content: 70.02

Gene sequence:

>894_bases
ATGAGTCCCACACCCCCTCGCAGCAAGTTTCCCCTGCCGCTGGATGCGGTAGTCCAGCGCAAGCCAGCACCGGCGCGTAC
CGCCGGCATGCCCGCGGTCGGTGCGCCGGGCCCTGCGCAGGCGCAGGCCAAAGCCCGCGACAAGCAGCCGTCGGCGCCCA
CGGCCGCGGCCAGCGCCGTAGAGGCGCGTGCCTCGGCGGCGACGGCGGGCGGGGGGGGCATGGCATCGGACCGCGCGCGC
GGCGCGCTGGCGGCGCGGCTGCGCGCCTCGGGTATCCGCGACGAACGCGTGCTGGCCGCCATTGGCACTGTGCCGCGCCA
TCTGTTTGTCGAGCCCGGGCTGGCATCGCAGGCGTATGAAGATGCAGCGCTGCCCATCGGCCATCAGCAGACGATCTCGA
AGCCTTCCGTGGTGGCGCGCATGATCGAACTGCTGCGCGAGGGCCTTTCGGCGGACACGCCGGTCGAGCGCGTGCTGGAG
ATCGGCACCGGCTGCGGTTATCAGGCCGCGGTGCTGAGCCTGGTGGCGCGTGAAGTTTTTTCCATCGAGCGCATCCGGCC
GTTGCACGAGCAGGCCAAGGCGAACCTGCGGCCGCTGCGTGTGCCGAACCTGCGGCTGCACTATGGCGACGGCATGCTAG
GCCTGCCGCAGGCTGCGCCGTTTTCGGCCATCATCCTTGCCGCCGCCGGCATGGAGGTGCCGCAGGCCCTGCTCGAGCAG
CTTGCCATCGGCGGCAGGCTGATCGCGCCCGTGGCGGTGGTTCCGCCAGCGGGCGGATCGGGCCAGACGGTCACGCAGCA
GTTGCTGCTGATCGAGCGGCTCAATCGGCATCGTTTTCACCGGACCGCGCTTGAAGCCGTTTTCTTTGTGCCCTTAAAAT
CGGGCACCATCTGA

Upstream 100 bases:

>100_bases
TCGCTGACGCCGTTGCAACTCGACCTCACACATCGTAGCCAGCTCGAGGCGCTTGCCCAGTGGCTGAACTAGCCATTCCC
GCCGCAGTGCCTCAGCGCCG

Downstream 100 bases:

>100_bases
GTCGAACTATGCACAAGATCGTGAATTCGCAACAATTTGCACGCGCAGGCCAGCTTGTGGCGGCTTCCACGCTGGTGGCG
CTGCTGGCAGCCTGCGCCAA

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSPTPPRSKFPLPLDAVVQRKPAPARTAGMPAVGAPGPAQAQAKARDKQPSAPTAAASAVEARASAATAGGGGMASDRAR
GALAARLRASGIRDERVLAAIGTVPRHLFVEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGLSADTPVERVLE
IGTGCGYQAAVLSLVAREVFSIERIRPLHEQAKANLRPLRVPNLRLHYGDGMLGLPQAAPFSAIILAAAGMEVPQALLEQ
LAIGGRLIAPVAVVPPAGGSGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI

Sequences:

>Translated_297_residues
MSPTPPRSKFPLPLDAVVQRKPAPARTAGMPAVGAPGPAQAQAKARDKQPSAPTAAASAVEARASAATAGGGGMASDRAR
GALAARLRASGIRDERVLAAIGTVPRHLFVEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGLSADTPVERVLE
IGTGCGYQAAVLSLVAREVFSIERIRPLHEQAKANLRPLRVPNLRLHYGDGMLGLPQAAPFSAIILAAAGMEVPQALLEQ
LAIGGRLIAPVAVVPPAGGSGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI
>Mature_296_residues
SPTPPRSKFPLPLDAVVQRKPAPARTAGMPAVGAPGPAQAQAKARDKQPSAPTAAASAVEARASAATAGGGGMASDRARG
ALAARLRASGIRDERVLAAIGTVPRHLFVEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGLSADTPVERVLEI
GTGCGYQAAVLSLVAREVFSIERIRPLHEQAKANLRPLRVPNLRLHYGDGMLGLPQAAPFSAIILAAAGMEVPQALLEQL
AIGGRLIAPVAVVPPAGGSGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=239, Percent_Identity=31.7991631799163, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789100, Length=226, Percent_Identity=43.3628318584071, Blast_Score=156, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71983477, Length=227, Percent_Identity=31.7180616740088, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=227, Percent_Identity=30.8370044052863, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17981723, Length=187, Percent_Identity=36.8983957219251, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_CUPPJ (Q46ZH2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296305.1
- ProteinModelPortal:   Q46ZH2
- SMR:   Q46ZH2
- GeneID:   3611351
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2097
- NMPDR:   fig|264198.3.peg.2783
- HOGENOM:   HBG699907
- OMA:   HEQAKAN
- ProtClustDB:   CLSK2302513
- BioCyc:   REUT264198:REUT_A2097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 31010; Mature: 30879

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTPPRSKFPLPLDAVVQRKPAPARTAGMPAVGAPGPAQAQAKARDKQPSAPTAAASAV
CCCCCCCCCCCCCHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
EARASAATAGGGGMASDRARGALAARLRASGIRDERVLAAIGTVPRHLFVEPGLASQAYE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEEECCCCCHHHHH
DAALPIGHQQTISKPSVVARMIELLREGLSADTPVERVLEIGTGCGYQAAVLSLVAREVF
HCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
SIERIRPLHEQAKANLRPLRVPNLRLHYGDGMLGLPQAAPFSAIILAAAGMEVPQALLEQ
HHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
LAIGGRLIAPVAVVPPAGGSGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI
HHHCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
SPTPPRSKFPLPLDAVVQRKPAPARTAGMPAVGAPGPAQAQAKARDKQPSAPTAAASAV
CCCCCCCCCCCCHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
EARASAATAGGGGMASDRARGALAARLRASGIRDERVLAAIGTVPRHLFVEPGLASQAYE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEEECCCCCHHHHH
DAALPIGHQQTISKPSVVARMIELLREGLSADTPVERVLEIGTGCGYQAAVLSLVAREVF
HCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
SIERIRPLHEQAKANLRPLRVPNLRLHYGDGMLGLPQAAPFSAIILAAAGMEVPQALLEQ
HHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
LAIGGRLIAPVAVVPPAGGSGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI
HHHCCEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA