Click here to switch to the map view.

The map label for this gene is rumA

Identifier: 73541780

GI number: 73541780

Start: 2299733

End: 2301124

Strand: Reverse

Name: rumA

Synonym: Reut_A2092

Alternate gene names: 73541780

Gene position: 2301124-2299733 (Counterclockwise)

Preceding gene: 73541782

Following gene: 73541778

Centisome position: 60.45

GC content: 65.23

Gene sequence:

>1392_bases
ATGCCGGCAGCGCCGCGTGCGGCATCTGCCTCTCCGGCTGCCGTCGCGCCCGGTTCCGATCCCGTGGTTTCCATTGACAG
CCTCGACATGGAGGCGCGCGGCGTCGGGCGCCTGGTCAATGAAGACGGCACTCCCGGCAAGGTCATCTTCGTCGAAGGTG
CGTTGCCCGGCGAAACCGTGAGCTACAAGAGCTTCCGCCGCAAGCCGAGCTACGAACAGGCCCATCTGCTGGAAGTGCGT
CGAGAATCGGTGATGCGTGTCAAGCCAGGTTGCAAGCACTTTGGCGTGTGCGGCGGATGCTCCATGCAGCACCTGGACTC
GCGCGCGCAGCTTGCGATCAAGCAGCGGGTACTCGAAGACAACCTCTGGCACCTGTCGAAGGTTCGTCCGGAAGTGATGT
TCCGGCCGATCGCCGGGCCGGACTGGGGCTATCGCTACCGCGCACGGCTGACGGTGCGGTATGTGGCTAAGAAGGGTGGG
GTACTGGTGGGTTTCCACGAGCGCAAGAGCAGCTACGTGGCGGACATGACGAGCTGCGAGATCCTTCCGCCACATATCTC
GGCGATGCTGGTGCCGTTGCGCGAACTGGTGACGGGCTTGTCCATTCGCGACCGCATGCCGCAGATCGAACTGGCGGTGG
GCCATGAGGTTACGGCGCTGGTGCTGCGCATCCTCGAACCGCTGACCGACGCGGACAAGGACCTGCTGCGCGCCTTCGCG
GACCAGCACAATGTCCAGTTCTGGCTCCAGCCCAAGGGCCCGGATACCGTTGTGCCCTTCTATCCGCTCGACCGTGAGCT
GGCCTATACGCTGCCCGAGTTCGGCATCCGTATGCCGTTCAAGCCGACCGACTTCACGCAGGTCAACCACCACATCAACA
GGGTACTGATCGGCCGCGCACTGCGCCTGCTGGACGTCCAGCCCCAAGACCGCCTGCTCGACCTGTTCTGCGGCATCGGC
AACTTCACGCTGCCACTCGCCACGCAGGGCCGCTCGGTCATGGGCATTGAAGGCAGCGAGGCGCTGACGACCCGGGCGCT
GGCGAATGCCGGCTACAACGGCCTGGCGGAAAAGACGGAATTCGCCTGCCGCAACCTGTTCGAAGTGACCGCCGAAGATA
TCGCCGCGCTGGGCCGCTTCGATCGCTGGTTGATCGACCCGCCGCGCGAAGGTGCGCTGGCGGTGTGCAAGGCGCTGGGC
GAGCTGTCGCAGCAGGGCAGCGACGTGCTGCCGCAACGCATCGTCTATGTGTCGTGCAGCCCGGCAACACTGGCGCGCGA
CGCGGGCCTGCTGGTGCATGAGGCCGGGTATCGCCTGGCAGGGGCGGGCGTCGTGAACATGTTCCCGCATACGTCGCACG
TCGAATCGATTGCCGTCTTCGAGCGGCGTTGA

Upstream 100 bases:

>100_bases
ACCCGGGCGTCGGAGCGCGCGTCTGGACTACAATCGCGCTTTCGCAAAACAATACGTCAGGTTTCGTCTGGTGTCCCAAG
CCGTGTCCTCCCCACAAGAA

Downstream 100 bases:

>100_bases
GCGCGCGACAACGCTAGCGCCCAATGAAAAACGCCGGCCAAGTGGCCGGCGTTCTTGTTTTACGCAGGACTGCGTCAGTC
GCGGCTGCCGCCGAAGATGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPAAPRAASASPAAVAPGSDPVVSIDSLDMEARGVGRLVNEDGTPGKVIFVEGALPGETVSYKSFRRKPSYEQAHLLEVR
RESVMRVKPGCKHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVRPEVMFRPIAGPDWGYRYRARLTVRYVAKKGG
VLVGFHERKSSYVADMTSCEILPPHISAMLVPLRELVTGLSIRDRMPQIELAVGHEVTALVLRILEPLTDADKDLLRAFA
DQHNVQFWLQPKGPDTVVPFYPLDRELAYTLPEFGIRMPFKPTDFTQVNHHINRVLIGRALRLLDVQPQDRLLDLFCGIG
NFTLPLATQGRSVMGIEGSEALTTRALANAGYNGLAEKTEFACRNLFEVTAEDIAALGRFDRWLIDPPREGALAVCKALG
ELSQQGSDVLPQRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERR

Sequences:

>Translated_463_residues
MPAAPRAASASPAAVAPGSDPVVSIDSLDMEARGVGRLVNEDGTPGKVIFVEGALPGETVSYKSFRRKPSYEQAHLLEVR
RESVMRVKPGCKHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVRPEVMFRPIAGPDWGYRYRARLTVRYVAKKGG
VLVGFHERKSSYVADMTSCEILPPHISAMLVPLRELVTGLSIRDRMPQIELAVGHEVTALVLRILEPLTDADKDLLRAFA
DQHNVQFWLQPKGPDTVVPFYPLDRELAYTLPEFGIRMPFKPTDFTQVNHHINRVLIGRALRLLDVQPQDRLLDLFCGIG
NFTLPLATQGRSVMGIEGSEALTTRALANAGYNGLAEKTEFACRNLFEVTAEDIAALGRFDRWLIDPPREGALAVCKALG
ELSQQGSDVLPQRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERR
>Mature_462_residues
PAAPRAASASPAAVAPGSDPVVSIDSLDMEARGVGRLVNEDGTPGKVIFVEGALPGETVSYKSFRRKPSYEQAHLLEVRR
ESVMRVKPGCKHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVRPEVMFRPIAGPDWGYRYRARLTVRYVAKKGGV
LVGFHERKSSYVADMTSCEILPPHISAMLVPLRELVTGLSIRDRMPQIELAVGHEVTALVLRILEPLTDADKDLLRAFAD
QHNVQFWLQPKGPDTVVPFYPLDRELAYTLPEFGIRMPFKPTDFTQVNHHINRVLIGRALRLLDVQPQDRLLDLFCGIGN
FTLPLATQGRSVMGIEGSEALTTRALANAGYNGLAEKTEFACRNLFEVTAEDIAALGRFDRWLIDPPREGALAVCKALGE
LSQQGSDVLPQRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=38.7892376681614, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1787083, Length=395, Percent_Identity=25.5696202531646, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_CUPPJ (Q46ZH7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296300.1
- ProteinModelPortal:   Q46ZH7
- SMR:   Q46ZH7
- GeneID:   3611346
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2092
- NMPDR:   fig|264198.3.peg.2778
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- ProtClustDB:   PRK13168
- BioCyc:   REUT264198:REUT_A2092-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 51046; Mature: 50914

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 419-419 BINDING 287-287 BINDING 316-316 BINDING 321-321 BINDING 337-337 BINDING 365-365 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAPRAASASPAAVAPGSDPVVSIDSLDMEARGVGRLVNEDGTPGKVIFVEGALPGETV
CCCCCCCCCCCCCEECCCCCCEEEEECCCCCHHCCHHHHCCCCCCCEEEEEECCCCCCCC
SYKSFRRKPSYEQAHLLEVRRESVMRVKPGCKHFGVCGGCSMQHLDSRAQLAIKQRVLED
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NLWHLSKVRPEVMFRPIAGPDWGYRYRARLTVRYVAKKGGVLVGFHERKSSYVADMTSCE
CHHHHHHCCHHHEEECCCCCCCCEEEEEEEEEEEEECCCCEEEEEHHCCCCHHHHCCCCC
ILPPHISAMLVPLRELVTGLSIRDRMPQIELAVGHEVTALVLRILEPLTDADKDLLRAFA
CCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
DQHNVQFWLQPKGPDTVVPFYPLDRELAYTLPEFGIRMPFKPTDFTQVNHHINRVLIGRA
CCCCEEEEEECCCCCCEEECCCCCHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRLLDVQPQDRLLDLFCGIGNFTLPLATQGRSVMGIEGSEALTTRALANAGYNGLAEKTE
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHH
FACRNLFEVTAEDIAALGRFDRWLIDPPREGALAVCKALGELSQQGSDVLPQRIVYVSCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERR
CHHHHHCCCEEEECCCCEEECCCEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
PAAPRAASASPAAVAPGSDPVVSIDSLDMEARGVGRLVNEDGTPGKVIFVEGALPGETV
CCCCCCCCCCCCEECCCCCCEEEEECCCCCHHCCHHHHCCCCCCCEEEEEECCCCCCCC
SYKSFRRKPSYEQAHLLEVRRESVMRVKPGCKHFGVCGGCSMQHLDSRAQLAIKQRVLED
CHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NLWHLSKVRPEVMFRPIAGPDWGYRYRARLTVRYVAKKGGVLVGFHERKSSYVADMTSCE
CHHHHHHCCHHHEEECCCCCCCCEEEEEEEEEEEEECCCCEEEEEHHCCCCHHHHCCCCC
ILPPHISAMLVPLRELVTGLSIRDRMPQIELAVGHEVTALVLRILEPLTDADKDLLRAFA
CCCHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHH
DQHNVQFWLQPKGPDTVVPFYPLDRELAYTLPEFGIRMPFKPTDFTQVNHHINRVLIGRA
CCCCEEEEEECCCCCCEEECCCCCHHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRLLDVQPQDRLLDLFCGIGNFTLPLATQGRSVMGIEGSEALTTRALANAGYNGLAEKTE
HHHHCCCCHHHHHHHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCHHHHH
FACRNLFEVTAEDIAALGRFDRWLIDPPREGALAVCKALGELSQQGSDVLPQRIVYVSCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECC
PATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERR
CHHHHHCCCEEEECCCCEEECCCEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA