| Definition | Ralstonia eutropha JMP134 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007347 |
| Length | 3,806,533 |
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The map label for this gene is ispG [H]
Identifier: 73541774
GI number: 73541774
Start: 2293862
End: 2295154
Strand: Reverse
Name: ispG [H]
Synonym: Reut_A2086
Alternate gene names: 73541774
Gene position: 2295154-2293862 (Counterclockwise)
Preceding gene: 73541775
Following gene: 73541773
Centisome position: 60.3
GC content: 65.66
Gene sequence:
>1293_bases ATGAACGAACAGCTTTGTCTTCCGGTCCTGCCGGGCCCGCTGCCGCGCCGCGAAAGGCGCCAGGCGAGCATCGCGTGGGG CGACAACATCGTCACCATCGGTGGCAGCGCGCCGGTGCGCGTGCAGTCGATGACCAATACGGATACCGTCGATGCCATTG GCACGGCCATCCAGGTCAAGGAACTGGCGCGCGCGGGCTCCGAGATCGTGCGCATCACGGTCAATACGCCCGAGGCAGCG GCAGCGGTGCCGCATATCCGCGAGCAGCTCGACCGCATGGGCGTCAACGTGCCGCTGGTGGGCGATTTCCACTACAACGG CCACAAGCTGCTGCAGGACTATCCGGCCTGCGCCGAGGCGCTGTCGAAGTACCGCATCAACCCCGGCAATGTGGGGCAGG GCGCCAAGCGCGACACGCAATTCGCGCAGATGATCGAGATGGCCTGCCGGTACAACAAGCCGGTGCGCATCGGCGTCAAC TGGGGCAGCCTGGACCAGGACCTGCTCGCGCGCATCATGGACGAGAACGCCGGCCGCGCGGAGCCGTGGCCCGCGCAGAG CGTGATGATCGAAGCGTTGATCACTTCGGCGATCGATTCGGCAAAGAAGGCCGAGGAAATCGGCCTGCCGGGCAACCAGA TCATCCTGTCGTGCAAGGTGTCGCAGGTGCAGGAACTGATTGCGGTGTACCGCGAACTCGCGCGCCGCTGCGACTATGCG CTGCACCTCGGCCTGACCGAGGCGGGCATGGGCAGCAAGGGCATCGTGGCGTCGACGGCGGCGCTGTCGGTGCTGCTGCA GGAAGGCATCGGCGACACGATCCGGATTTCGCTGACGCCGGAACCCGGTGCGCCGCGCGAGAAGGAAGTCTATGTCGGGC AGGAAATCCTGCAGACCATGGGCATGCGGAATTTCACGCCGATGGTGATTGCGTGCCCCGGCTGCGGCCGCACGACGAGC ACCACGTTCCAGGAACTGGCGGCAAGCATTCAGGCGTACCTGCGCGAGCAGATGCCGCAGTGGAAGACCGCTTATCCGGG CGTCGAAGAGATGGATGTGGCCGTGATGGGCTGCATCGTCAACGGCCCTGGCGAAAGCAAGCATGCCAACATCGGCATTT CGCTGCCGGGTTCGGGCGAATCGCCGGCGGCGCCGGTGTTTGTCGATGGCGTCAAGGTCAAGACGCTGCGCGGCGAGCGC ATCGCCGAGGAATTCCAGGCGATCGTCGACGAGTACGTTCGCACGCATTACGGTCCGGGCGCAGCGCGGGCAGAGAAGGA AGCGGTGGCCTGA
Upstream 100 bases:
>100_bases ACGCCGGTTGACCTGAAGGCGGCGAATCGCAACAACGTGGCGCGGTTGACGCTGCCCTGACGCGGCTCGACACCGTGCAT CATTGCGAGGTGGTAACGCT
Downstream 100 bases:
>100_bases GCCGGCATTGGCCAGTCCACCCAGCAAGATAACGACAGAATGACGCAAAACGAGAATCCGTCCGCGCAAAGCGGCGCCAA GCCAGAAGACAAGGCACGCC
Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Products: NA
Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]
Number of amino acids: Translated: 430; Mature: 430
Protein sequence:
>430_residues MNEQLCLPVLPGPLPRRERRQASIAWGDNIVTIGGSAPVRVQSMTNTDTVDAIGTAIQVKELARAGSEIVRITVNTPEAA AAVPHIREQLDRMGVNVPLVGDFHYNGHKLLQDYPACAEALSKYRINPGNVGQGAKRDTQFAQMIEMACRYNKPVRIGVN WGSLDQDLLARIMDENAGRAEPWPAQSVMIEALITSAIDSAKKAEEIGLPGNQIILSCKVSQVQELIAVYRELARRCDYA LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGMRNFTPMVIACPGCGRTTS TTFQELAASIQAYLREQMPQWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGER IAEEFQAIVDEYVRTHYGPGAARAEKEAVA
Sequences:
>Translated_430_residues MNEQLCLPVLPGPLPRRERRQASIAWGDNIVTIGGSAPVRVQSMTNTDTVDAIGTAIQVKELARAGSEIVRITVNTPEAA AAVPHIREQLDRMGVNVPLVGDFHYNGHKLLQDYPACAEALSKYRINPGNVGQGAKRDTQFAQMIEMACRYNKPVRIGVN WGSLDQDLLARIMDENAGRAEPWPAQSVMIEALITSAIDSAKKAEEIGLPGNQIILSCKVSQVQELIAVYRELARRCDYA LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGMRNFTPMVIACPGCGRTTS TTFQELAASIQAYLREQMPQWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGER IAEEFQAIVDEYVRTHYGPGAARAEKEAVA >Mature_430_residues MNEQLCLPVLPGPLPRRERRQASIAWGDNIVTIGGSAPVRVQSMTNTDTVDAIGTAIQVKELARAGSEIVRITVNTPEAA AAVPHIREQLDRMGVNVPLVGDFHYNGHKLLQDYPACAEALSKYRINPGNVGQGAKRDTQFAQMIEMACRYNKPVRIGVN WGSLDQDLLARIMDENAGRAEPWPAQSVMIEALITSAIDSAKKAEEIGLPGNQIILSCKVSQVQELIAVYRELARRCDYA LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTMGMRNFTPMVIACPGCGRTTS TTFQELAASIQAYLREQMPQWKTAYPGVEEMDVAVMGCIVNGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGER IAEEFQAIVDEYVRTHYGPGAARAEKEAVA
Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]
COG id: COG0821
COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ispG family [H]
Homologues:
Organism=Escherichia coli, GI1788863, Length=393, Percent_Identity=35.1145038167939, Blast_Score=201, Evalue=1e-52,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016425 - InterPro: IPR004588 [H]
Pfam domain/function: PF04551 GcpE [H]
EC number: =1.17.7.1 [H]
Molecular weight: Translated: 46357; Mature: 46357
Theoretical pI: Translated: 5.52; Mature: 5.52
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.9 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 5.1 %Cys+Met (Translated Protein) 1.9 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 5.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNEQLCLPVLPGPLPRRERRQASIAWGDNIVTIGGSAPVRVQSMTNTDTVDAIGTAIQVK CCCCEEEECCCCCCCCHHHHCEEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH ELARAGSEIVRITVNTPEAAAAVPHIREQLDRMGVNVPLVGDFHYNGHKLLQDYPACAEA HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH LSKYRINPGNVGQGAKRDTQFAQMIEMACRYNKPVRIGVNWGSLDQDLLARIMDENAGRA HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCC EPWPAQSVMIEALITSAIDSAKKAEEIGLPGNQIILSCKVSQVQELIAVYRELARRCDYA CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCE LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTM EEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC GMRNFTPMVIACPGCGRTTSTTFQELAASIQAYLREQMPQWKTAYPGVEEMDVAVMGCIV CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEEEEE NGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGERIAEEFQAIVDEYVRTHYGPG CCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCC AARAEKEAVA HHHCHHHCCC >Mature Secondary Structure MNEQLCLPVLPGPLPRRERRQASIAWGDNIVTIGGSAPVRVQSMTNTDTVDAIGTAIQVK CCCCEEEECCCCCCCCHHHHCEEEEECCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHH ELARAGSEIVRITVNTPEAAAAVPHIREQLDRMGVNVPLVGDFHYNGHKLLQDYPACAEA HHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHH LSKYRINPGNVGQGAKRDTQFAQMIEMACRYNKPVRIGVNWGSLDQDLLARIMDENAGRA HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCC EPWPAQSVMIEALITSAIDSAKKAEEIGLPGNQIILSCKVSQVQELIAVYRELARRCDYA CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCE LHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGAPREKEVYVGQEILQTM EEECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHC GMRNFTPMVIACPGCGRTTSTTFQELAASIQAYLREQMPQWKTAYPGVEEMDVAVMGCIV CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCEEEEEEEE NGPGESKHANIGISLPGSGESPAAPVFVDGVKVKTLRGERIAEEFQAIVDEYVRTHYGPG CCCCCCCCCEEEEECCCCCCCCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCC AARAEKEAVA HHHCHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11823852 [H]