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The map label for this gene is hisS [H]

Identifier: 73541773

GI number: 73541773

Start: 2292452

End: 2293822

Strand: Reverse

Name: hisS [H]

Synonym: Reut_A2085

Alternate gene names: 73541773

Gene position: 2293822-2292452 (Counterclockwise)

Preceding gene: 73541774

Following gene: 73541772

Centisome position: 60.26

GC content: 65.57

Gene sequence:

>1371_bases
ATGACGCAAAACGAGAATCCGTCCGCGCAAAGCGGCGCCAAGCCAGAAGACAAGGCACGCCCCGCGAAGGCCTTGCAGGG
CGTGAAGGGCATGAACGACATGCTGCCCGCCGATGCCCCGCTGTGGGAGCATTTCGAGAACGCCGCGCGTGCGATGCTGC
GCGCCTACGGCTACCAGCAGATCCGCACGCCGATCGTCGAGCATACCCAGCTGTTCGTGCGCGGTATCGGCGAGGTCACG
GATATCGTGGAGAAGGAGATGTATTCCTTCACCGATGCGCTCAACGGTGAACAACTGACGCTGCGTCCGGAAGGCACTGC
CGCCGCCGTGCGTGCGACCATCGAGCACAACCTGCTGTACGACGGCCCGAAGCGCCTGTGGTACACCGGCCCGATGTTCC
GCCACGAGCGCCCGCAGCGCGGCCGCTATCGCCAGTTCCACCAGCTCGGCGCTGAGGCGCTCGGCTTTGCGGGCCCGGAT
GTGGATGCCGAGATCATCCTGATGTGCCAGCGCCTGTGGGACGACCTGGGCCTGACCGGCGTGCGGCTGGAGATCAACTC
GCTGGGCCAGGCGCACGAGCGCGCCGCGCACCGCGAGGAGCTGATCAAGTACCTCGAAGGCTTCAAGGACATCCTGGACG
AGGACGGCAAGCGCCGCCTCTACACGAATCCGCTGCGCGTGCTGGATACGAAGAATCCCGCGCTGCAGGAAATGGCCGCC
AACGCGCCCAAGCTGATCGATTTCCTCGGTGAGGAATCGCTCGCCCACTTCGAGGGCGTGCAGCGGCTGCTCAAGGCCAA
CAACATCCCGTACAAGATCAACCCGCGCCTGGTGCGTGGGCTGGACTACTACAACCTGACGGTGTTCGAGTGGATCACCG
ACAAGCTCGGTGCGCAGGGCACCATCGCCGGTGGCGGCCGCTATGACCCGCTGATTGCGCAGATGGGCGGCAAGGCGGCG
CCGGCTTGCGGCTGGGCCATGGGCATCGAGCGCATCATCGAGCTGATCCGCGAGGAAGGCGTGGTGCCTGATGCGGTCGG
TTGCGACGTCTATCTGGTGCATCAGGGCGAAGCGGCGCAGCAGCAGGCGACGGTTGCCGCCGAGCGCCTGCGTGATGCCG
GCCTGGACGTGGTGCTGCATGCCACGCCCGATGGCAAGAGCGGCAGCTTCAAGTCGCAGATGAAGCGCGCTGACGCGAGC
GGTGCGGCGTATGCCGTGATCATTGGCGAAGATGAAGTCGCTGCGGGCGTGGTGCAGGTGAAGGAACTTCGCCAGGGCGC
GCAGGCTGAAGGTGGTGGCCAGCAGGCGCAGGTGCCCGCTGAAAACCTGGTCGATTACCTGATCGACGCGATGGTCGGCG
CCAGCGAATAA

Upstream 100 bases:

>100_bases
GTACGTTCGCACGCATTACGGTCCGGGCGCAGCGCGGGCAGAGAAGGAAGCGGTGGCCTGAGCCGGCATTGGCCAGTCCA
CCCAGCAAGATAACGACAGA

Downstream 100 bases:

>100_bases
CGCGACGACTGACGACGCAAGTAACGGCGTAGCAAGATCCGCCCGGCAGGAAGCGAAGCCTGCCGGAAGCGGCCTGAGAT
ACAAACCAACCGCAAAAAGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTQNENPSAQSGAKPEDKARPAKALQGVKGMNDMLPADAPLWEHFENAARAMLRAYGYQQIRTPIVEHTQLFVRGIGEVT
DIVEKEMYSFTDALNGEQLTLRPEGTAAAVRATIEHNLLYDGPKRLWYTGPMFRHERPQRGRYRQFHQLGAEALGFAGPD
VDAEIILMCQRLWDDLGLTGVRLEINSLGQAHERAAHREELIKYLEGFKDILDEDGKRRLYTNPLRVLDTKNPALQEMAA
NAPKLIDFLGEESLAHFEGVQRLLKANNIPYKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIAQMGGKAA
PACGWAMGIERIIELIREEGVVPDAVGCDVYLVHQGEAAQQQATVAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADAS
GAAYAVIIGEDEVAAGVVQVKELRQGAQAEGGGQQAQVPAENLVDYLIDAMVGASE

Sequences:

>Translated_456_residues
MTQNENPSAQSGAKPEDKARPAKALQGVKGMNDMLPADAPLWEHFENAARAMLRAYGYQQIRTPIVEHTQLFVRGIGEVT
DIVEKEMYSFTDALNGEQLTLRPEGTAAAVRATIEHNLLYDGPKRLWYTGPMFRHERPQRGRYRQFHQLGAEALGFAGPD
VDAEIILMCQRLWDDLGLTGVRLEINSLGQAHERAAHREELIKYLEGFKDILDEDGKRRLYTNPLRVLDTKNPALQEMAA
NAPKLIDFLGEESLAHFEGVQRLLKANNIPYKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIAQMGGKAA
PACGWAMGIERIIELIREEGVVPDAVGCDVYLVHQGEAAQQQATVAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADAS
GAAYAVIIGEDEVAAGVVQVKELRQGAQAEGGGQQAQVPAENLVDYLIDAMVGASE
>Mature_455_residues
TQNENPSAQSGAKPEDKARPAKALQGVKGMNDMLPADAPLWEHFENAARAMLRAYGYQQIRTPIVEHTQLFVRGIGEVTD
IVEKEMYSFTDALNGEQLTLRPEGTAAAVRATIEHNLLYDGPKRLWYTGPMFRHERPQRGRYRQFHQLGAEALGFAGPDV
DAEIILMCQRLWDDLGLTGVRLEINSLGQAHERAAHREELIKYLEGFKDILDEDGKRRLYTNPLRVLDTKNPALQEMAAN
APKLIDFLGEESLAHFEGVQRLLKANNIPYKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIAQMGGKAAP
ACGWAMGIERIIELIREEGVVPDAVGCDVYLVHQGEAAQQQATVAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASG
AAYAVIIGEDEVAAGVVQVKELRQGAQAEGGGQQAQVPAENLVDYLIDAMVGASE

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=412, Percent_Identity=54.8543689320388, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71993686, Length=503, Percent_Identity=23.0616302186879, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993693, Length=503, Percent_Identity=23.0616302186879, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50053; Mature: 49922

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNENPSAQSGAKPEDKARPAKALQGVKGMNDMLPADAPLWEHFENAARAMLRAYGYQQ
CCCCCCCCCCCCCCCCHHCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHH
IRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDALNGEQLTLRPEGTAAAVRATIEHNLLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCEE
DGPKRLWYTGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWDDLGLTG
CCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCE
VRLEINSLGQAHERAAHREELIKYLEGFKDILDEDGKRRLYTNPLRVLDTKNPALQEMAA
EEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHEEECCCHHHHHHHC
NAPKLIDFLGEESLAHFEGVQRLLKANNIPYKINPRLVRGLDYYNLTVFEWITDKLGAQG
CCHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHHHHHHHHCCCE
TIAGGGRYDPLIAQMGGKAAPACGWAMGIERIIELIREEGVVPDAVGCDVYLVHQGEAAQ
EECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHH
QQATVAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGAAYAVIIGEDEVAAGVVQV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
KELRQGAQAEGGGQQAQVPAENLVDYLIDAMVGASE
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQNENPSAQSGAKPEDKARPAKALQGVKGMNDMLPADAPLWEHFENAARAMLRAYGYQQ
CCCCCCCCCCCCCCCHHCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHH
IRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDALNGEQLTLRPEGTAAAVRATIEHNLLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCEE
DGPKRLWYTGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWDDLGLTG
CCCCCEEECCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCE
VRLEINSLGQAHERAAHREELIKYLEGFKDILDEDGKRRLYTNPLRVLDTKNPALQEMAA
EEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHEEECCCHHHHHHHC
NAPKLIDFLGEESLAHFEGVQRLLKANNIPYKINPRLVRGLDYYNLTVFEWITDKLGAQG
CCHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEECCHHHHCCCCCCHHHHHHHHHHHCCCE
TIAGGGRYDPLIAQMGGKAAPACGWAMGIERIIELIREEGVVPDAVGCDVYLVHQGEAAQ
EECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHH
QQATVAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGAAYAVIIGEDEVAAGVVQV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHH
KELRQGAQAEGGGQQAQVPAENLVDYLIDAMVGASE
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA