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The map label for this gene is emrB [H]

Identifier: 73541713

GI number: 73541713

Start: 2220967

End: 2222568

Strand: Direct

Name: emrB [H]

Synonym: Reut_A2025

Alternate gene names: 73541713

Gene position: 2220967-2222568 (Clockwise)

Preceding gene: 73541712

Following gene: 73541722

Centisome position: 58.35

GC content: 64.73

Gene sequence:

>1602_bases
ATGGCGGACGCACTGACCGTGGCTGAACGCGCCGGCGCCGCACCAAAGCTGCCGGCGCGCCCCGCGCAGCCGCAGCCGCT
GAGCGGCGGCAAGCTGGTGATCGGCACCATCGCGCTGTCGCTGGCCACGTTCATGAACGTGCTGGATTCGTCGATCGCCA
ACGTGTCGATCCCGGCGATTTCAGGCGACCTCGGCGTGGCGCCGAACCAGGGCACCTGGGTCATCACCTCGTTCGCGGTG
GCCAACGCGATCTCGGTGCCGCTGACGGGCTGGCTCACCACGCGCTTCGGCGCGGTGCGCCTGTTCATCACGTCGATCCT
GCTGTTCGTGCTGTCGTCGTGGCTGTGCGGCGTCGCGCCTAACCTGGAAACGCTGCTCGCAGCACGTATCCTCCAGGGCG
CCGTGGCCGGGCCGATGATCCCGCTGTCGCAGTCGCTGCTGCTGTCGAGCTACCCGCCGCAGAAGAGCACCATGGCGCTC
GCGCTGTGGGGCATGACCACGCTCGTGGCGCCGATCATGGGGCCGCTGCTCGGCGGCTGGATCTCGGACAACATGACGTG
GCCGTGGATCTTCTATATCAACGTGCCGGTGGGCCTGCTTACCGCGTACGCCACGTGGGCGATCTACAAGGACCGTGAAA
CGCCAACCAAGGTGCTGCCGATCGACCGCATCGGGCTCGCGCTGCTGGTGATCTGGGTGGGTTCGATGCAGCTCATGCTC
GACAAGGGCAAGGAGCTCGACTGGTTCCACTCGACCGAGATCGTGGTACTTACGCTGGTGGCCGTGCTCGGCTTCCTGTT
CTTCCTGGTATGGGAGACCTACGAGAAGCATCCGATCGTCGACATCACCCTGTTCAAGGGCCGGAACTTCAGCTCGGGCG
TGGTGGCGATCTCGGTCGCATACGGGCTGTTCTTCGGCAACCTGGTGATCCTGCCGTTGTGGTTGCAGACCATCGTGGGC
TACACGGCCACCGACGCCGGCATTGTGATGGCGCCCGTGGGGATCTTCGCGATCCTGCTGTCGCCGCTCATCGGACGCAA
CCTGCCGAAGATGGACGCACGGTGGGTCGCCACGGCAGCGTTCATCACGTTCGGCATCGTGAGCCTCATGCGTGCCGGAT
TCACCACGCAAGTGGACACCGTCACGCTGATGATCCCTACGCTGATCCAGGGCGCCGCCATGGCGATGTTCTTCATCCCG
CTCACGTCGATCATCCTGTCCGGCCAGCCACCGGAGAAAATCCCGGCGGCGTCCGGCCTGTCGAACTTCGTGCGTATCAC
GTTCGGTGGTATCGGCGCGTCGATTTCGACCACGGTGTGGGAGAACCGCTCGGCCCTGCACCACGCGCAACTGGTGGAGA
AGATCAATCCCTACAACCCGGCCTACTCCGAGCAACTGCACCACCTGATGCAGATGGGCATGAACCAGGCACAGGCCGTC
GGCGTCATGGAGCGCAACATCAGCCAGCAGGCGGCGATGCTCGGCGCGAACGACATCTTCTGGATCTCGGGCGTACTGTT
CTTCGTGCTGATTGGCTTCGTGTGGCTCACCAAGCCGGGAAAGGGCGGCGCAGGAGCAGACGCCGCAGCCGGCGCACATT
GA

Upstream 100 bases:

>100_bases
CCGCAGATGCCGTACCTGCCAAGGGCGCACCGGTCATCAATGTTCCGGCCGATGACGCGGCCAGCGCCGCTGTGGCGCGG
CCTTCCAACCGCGGCTGATC

Downstream 100 bases:

>100_bases
CAGGAACAGACGGAAACATCCGGGAACAGACCGGAACAGGCGGGAACAGGCGGGAACAGGCGGGAACAGGCGGGAACAGG
CGGGAACAGCGGGAACAGGC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MADALTVAERAGAAPKLPARPAQPQPLSGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAV
ANAISVPLTGWLTTRFGAVRLFITSILLFVLSSWLCGVAPNLETLLAARILQGAVAGPMIPLSQSLLLSSYPPQKSTMAL
ALWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPVGLLTAYATWAIYKDRETPTKVLPIDRIGLALLVIWVGSMQLML
DKGKELDWFHSTEIVVLTLVAVLGFLFFLVWETYEKHPIVDITLFKGRNFSSGVVAISVAYGLFFGNLVILPLWLQTIVG
YTATDAGIVMAPVGIFAILLSPLIGRNLPKMDARWVATAAFITFGIVSLMRAGFTTQVDTVTLMIPTLIQGAAMAMFFIP
LTSIILSGQPPEKIPAASGLSNFVRITFGGIGASISTTVWENRSALHHAQLVEKINPYNPAYSEQLHHLMQMGMNQAQAV
GVMERNISQQAAMLGANDIFWISGVLFFVLIGFVWLTKPGKGGAGADAAAGAH

Sequences:

>Translated_533_residues
MADALTVAERAGAAPKLPARPAQPQPLSGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAV
ANAISVPLTGWLTTRFGAVRLFITSILLFVLSSWLCGVAPNLETLLAARILQGAVAGPMIPLSQSLLLSSYPPQKSTMAL
ALWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPVGLLTAYATWAIYKDRETPTKVLPIDRIGLALLVIWVGSMQLML
DKGKELDWFHSTEIVVLTLVAVLGFLFFLVWETYEKHPIVDITLFKGRNFSSGVVAISVAYGLFFGNLVILPLWLQTIVG
YTATDAGIVMAPVGIFAILLSPLIGRNLPKMDARWVATAAFITFGIVSLMRAGFTTQVDTVTLMIPTLIQGAAMAMFFIP
LTSIILSGQPPEKIPAASGLSNFVRITFGGIGASISTTVWENRSALHHAQLVEKINPYNPAYSEQLHHLMQMGMNQAQAV
GVMERNISQQAAMLGANDIFWISGVLFFVLIGFVWLTKPGKGGAGADAAAGAH
>Mature_532_residues
ADALTVAERAGAAPKLPARPAQPQPLSGGKLVIGTIALSLATFMNVLDSSIANVSIPAISGDLGVAPNQGTWVITSFAVA
NAISVPLTGWLTTRFGAVRLFITSILLFVLSSWLCGVAPNLETLLAARILQGAVAGPMIPLSQSLLLSSYPPQKSTMALA
LWGMTTLVAPIMGPLLGGWISDNMTWPWIFYINVPVGLLTAYATWAIYKDRETPTKVLPIDRIGLALLVIWVGSMQLMLD
KGKELDWFHSTEIVVLTLVAVLGFLFFLVWETYEKHPIVDITLFKGRNFSSGVVAISVAYGLFFGNLVILPLWLQTIVGY
TATDAGIVMAPVGIFAILLSPLIGRNLPKMDARWVATAAFITFGIVSLMRAGFTTQVDTVTLMIPTLIQGAAMAMFFIPL
TSIILSGQPPEKIPAASGLSNFVRITFGGIGASISTTVWENRSALHHAQLVEKINPYNPAYSEQLHHLMQMGMNQAQAVG
VMERNISQQAAMLGANDIFWISGVLFFVLIGFVWLTKPGKGGAGADAAAGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=55.0200803212851, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=45.6913827655311, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790195, Length=424, Percent_Identity=27.8301886792453, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87081983, Length=436, Percent_Identity=24.7706422018349, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788392, Length=328, Percent_Identity=29.2682926829268, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=200, Percent_Identity=26, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57018; Mature: 56887

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADALTVAERAGAAPKLPARPAQPQPLSGGKLVIGTIALSLATFMNVLDSSIANVSIPAI
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEECCEE
SGDLGVAPNQGTWVITSFAVANAISVPLTGWLTTRFGAVRLFITSILLFVLSSWLCGVAP
CCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLETLLAARILQGAVAGPMIPLSQSLLLSSYPPQKSTMALALWGMTTLVAPIMGPLLGGW
CHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISDNMTWPWIFYINVPVGLLTAYATWAIYKDRETPTKVLPIDRIGLALLVIWVGSMQLML
HCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHH
DKGKELDWFHSTEIVVLTLVAVLGFLFFLVWETYEKHPIVDITLFKGRNFSSGVVAISVA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
YGLFFGNLVILPLWLQTIVGYTATDAGIVMAPVGIFAILLSPLIGRNLPKMDARWVATAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
FITFGIVSLMRAGFTTQVDTVTLMIPTLIQGAAMAMFFIPLTSIILSGQPPEKIPAASGL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SNFVRITFGGIGASISTTVWENRSALHHAQLVEKINPYNPAYSEQLHHLMQMGMNQAQAV
CCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHH
GVMERNISQQAAMLGANDIFWISGVLFFVLIGFVWLTKPGKGGAGADAAAGAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADALTVAERAGAAPKLPARPAQPQPLSGGKLVIGTIALSLATFMNVLDSSIANVSIPAI
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCEECCEE
SGDLGVAPNQGTWVITSFAVANAISVPLTGWLTTRFGAVRLFITSILLFVLSSWLCGVAP
CCCCCCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLETLLAARILQGAVAGPMIPLSQSLLLSSYPPQKSTMALALWGMTTLVAPIMGPLLGGW
CHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ISDNMTWPWIFYINVPVGLLTAYATWAIYKDRETPTKVLPIDRIGLALLVIWVGSMQLML
HCCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHH
DKGKELDWFHSTEIVVLTLVAVLGFLFFLVWETYEKHPIVDITLFKGRNFSSGVVAISVA
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
YGLFFGNLVILPLWLQTIVGYTATDAGIVMAPVGIFAILLSPLIGRNLPKMDARWVATAA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
FITFGIVSLMRAGFTTQVDTVTLMIPTLIQGAAMAMFFIPLTSIILSGQPPEKIPAASGL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SNFVRITFGGIGASISTTVWENRSALHHAQLVEKINPYNPAYSEQLHHLMQMGMNQAQAV
CCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHH
GVMERNISQQAAMLGANDIFWISGVLFFVLIGFVWLTKPGKGGAGADAAAGAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]