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The map label for this gene is emrA [H]

Identifier: 73541712

GI number: 73541712

Start: 2219675

End: 2220964

Strand: Direct

Name: emrA [H]

Synonym: Reut_A2024

Alternate gene names: 73541712

Gene position: 2219675-2220964 (Clockwise)

Preceding gene: 73541711

Following gene: 73541713

Centisome position: 58.31

GC content: 68.06

Gene sequence:

>1290_bases
ATGAGCGATAACCAGCAAGCCGGCAACCCGAACACTCCGGCGGCATCCGTCAACAACAATGGCAAGCGCAAGCGCCTGTT
GCTTTCCCTGACCGCGGCCATCGTCGTGGCAGGCGTCGGCTACGGCGTGTACTGGGGCCTGTACGCGCGCCACTTCGAAA
GTACCGATGACGCGTACGTCGCCGGCAACGTGGTCCAGGTCACGCCGCTGGTAGGCGGCACCGTGGTGTCGATCGCCGCG
GATGACACGCAGCTCGTCACCGCAGGCCAGCCGCTGATCCAGCTCGACCGCGCCGATACGCAAGTCTCGCTGGAACAGGC
CGAGGCCATGCTGGCGCAGGCCGTGCGCGAAGTCCGCACGCTCTACGTCAACAACGGCTCGCTCGCCGCGAACGTGGCGC
TGCGCGAGGCCGACGTCGCCAAGGCGCGCGACGACCTGCGCCGCCGCCAGGCGATCGCCGGCTCGGGTGCGGTGTCGGGC
GAAGAAATCTCCCACGCACAGACCGCGCTGCAGGGGGCGCAATCGGCTCTGCTTGCCGCACGCGAGCAGCTCGCCTCGAA
CAAGGTGCTGACCGACCAGACCACCGTCGAAAAGCATCCGAACGTGCAGCGCGCGGCAGCCAACCTGCGCTCGGCCTATC
TGTCGTTCGCGCGTTCCGCTCTGCCCGCGCCGGTCACGGGCTACGTCGCCAAGCGCTCGGTCCAGGTCGGCCAGCGTGTG
GCCGCCGGGGCACCGCTGATGGCCATCGTGCCGCTGGACCAGGTCTGGGTCGACGCGAACTTCAAGGAAGTGCAGATCAC
GCATATGCGCATCGGCCAGCCCGTGACGCTGGAAGCCGACGTCTATGGTTCGAAGGTCAAGTACAACGGCAAGGTCGCGG
GCTTCTCTGCCGGTACCGGCTCCGCGTTCTCGCTGCTGCCCGCACAGAACGCCACCGGCAACTGGATCAAGGTGGTGCAG
CGCCTGCCCGTGCGTATCGCGCTTGACCCGCAGCAGCTCAAGGATCACCCGCTGCGCGTCGGCCTGTCGATGAACGTGAC
CGTCGACGTGCGCCACGAAGAAGGCGCCGCCATGGTCAGCGCCGCCGCGGCCAAGCCGATGCAGACGGACGTCTACGACA
AGGTCGCGCAAGACGCCGACCAGCTGATCGCGCGCATCATCGCCGTGAACAATGGCGGCCGCGCGCCAGCTCCCGCAGAT
GCCGTACCTGCCAAGGGCGCACCGGTCATCAATGTTCCGGCCGATGACGCGGCCAGCGCCGCTGTGGCGCGGCCTTCCAA
CCGCGGCTGA

Upstream 100 bases:

>100_bases
CATGCCCCCGCCGCCGCAGCGAACCAGACAACCAGCCAGGCCGGCGCACGCGGCTGATCCGCGCGCCCGCCGTCGCAGAA
CAGATTCACGAGAGACCAAA

Downstream 100 bases:

>100_bases
TCATGGCGGACGCACTGACCGTGGCTGAACGCGCCGGCGCCGCACCAAAGCTGCCGGCGCGCCCCGCGCAGCCGCAGCCG
CTGAGCGGCGGCAAGCTGGT

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSDNQQAGNPNTPAASVNNNGKRKRLLLSLTAAIVVAGVGYGVYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAA
DDTQLVTAGQPLIQLDRADTQVSLEQAEAMLAQAVREVRTLYVNNGSLAANVALREADVAKARDDLRRRQAIAGSGAVSG
EEISHAQTALQGAQSALLAAREQLASNKVLTDQTTVEKHPNVQRAAANLRSAYLSFARSALPAPVTGYVAKRSVQVGQRV
AAGAPLMAIVPLDQVWVDANFKEVQITHMRIGQPVTLEADVYGSKVKYNGKVAGFSAGTGSAFSLLPAQNATGNWIKVVQ
RLPVRIALDPQQLKDHPLRVGLSMNVTVDVRHEEGAAMVSAAAAKPMQTDVYDKVAQDADQLIARIIAVNNGGRAPAPAD
AVPAKGAPVINVPADDAASAAVARPSNRG

Sequences:

>Translated_429_residues
MSDNQQAGNPNTPAASVNNNGKRKRLLLSLTAAIVVAGVGYGVYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAA
DDTQLVTAGQPLIQLDRADTQVSLEQAEAMLAQAVREVRTLYVNNGSLAANVALREADVAKARDDLRRRQAIAGSGAVSG
EEISHAQTALQGAQSALLAAREQLASNKVLTDQTTVEKHPNVQRAAANLRSAYLSFARSALPAPVTGYVAKRSVQVGQRV
AAGAPLMAIVPLDQVWVDANFKEVQITHMRIGQPVTLEADVYGSKVKYNGKVAGFSAGTGSAFSLLPAQNATGNWIKVVQ
RLPVRIALDPQQLKDHPLRVGLSMNVTVDVRHEEGAAMVSAAAAKPMQTDVYDKVAQDADQLIARIIAVNNGGRAPAPAD
AVPAKGAPVINVPADDAASAAVARPSNRG
>Mature_428_residues
SDNQQAGNPNTPAASVNNNGKRKRLLLSLTAAIVVAGVGYGVYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAAD
DTQLVTAGQPLIQLDRADTQVSLEQAEAMLAQAVREVRTLYVNNGSLAANVALREADVAKARDDLRRRQAIAGSGAVSGE
EISHAQTALQGAQSALLAAREQLASNKVLTDQTTVEKHPNVQRAAANLRSAYLSFARSALPAPVTGYVAKRSVQVGQRVA
AGAPLMAIVPLDQVWVDANFKEVQITHMRIGQPVTLEADVYGSKVKYNGKVAGFSAGTGSAFSLLPAQNATGNWIKVVQR
LPVRIALDPQQLKDHPLRVGLSMNVTVDVRHEEGAAMVSAAAAKPMQTDVYDKVAQDADQLIARIIAVNNGGRAPAPADA
VPAKGAPVINVPADDAASAAVARPSNRG

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=393, Percent_Identity=45.0381679389313, Blast_Score=341, Evalue=6e-95,
Organism=Escherichia coli, GI1788711, Length=345, Percent_Identity=42.3188405797101, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1790519, Length=306, Percent_Identity=29.7385620915033, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081951, Length=305, Percent_Identity=26.2295081967213, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789637, Length=311, Percent_Identity=26.6881028938907, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44946; Mature: 44815

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQQAGNPNTPAASVNNNGKRKRLLLSLTAAIVVAGVGYGVYWGLYARHFESTDDAYV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEE
AGNVVQVTPLVGGTVVSIAADDTQLVTAGQPLIQLDRADTQVSLEQAEAMLAQAVREVRT
ECCEEEEEECCCCEEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHE
LYVNNGSLAANVALREADVAKARDDLRRRQAIAGSGAVSGEEISHAQTALQGAQSALLAA
EEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REQLASNKVLTDQTTVEKHPNVQRAAANLRSAYLSFARSALPAPVTGYVAKRSVQVGQRV
HHHHHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AAGAPLMAIVPLDQVWVDANFKEVQITHMRIGQPVTLEADVYGSKVKYNGKVAGFSAGTG
HCCCCEEEEEECHHEEECCCCCEEEEEEEECCCCEEEEECCCCCEEEECCEEEEECCCCC
SAFSLLPAQNATGNWIKVVQRLPVRIALDPQQLKDHPLRVGLSMNVTVDVRHEEGAAMVS
CEEEEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEEEEEEEEECCCCHHHH
AAAAKPMQTDVYDKVAQDADQLIARIIAVNNGGRAPAPADAVPAKGAPVINVPADDAASA
HHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
AVARPSNRG
HHCCCCCCC
>Mature Secondary Structure 
SDNQQAGNPNTPAASVNNNGKRKRLLLSLTAAIVVAGVGYGVYWGLYARHFESTDDAYV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCEE
AGNVVQVTPLVGGTVVSIAADDTQLVTAGQPLIQLDRADTQVSLEQAEAMLAQAVREVRT
ECCEEEEEECCCCEEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHE
LYVNNGSLAANVALREADVAKARDDLRRRQAIAGSGAVSGEEISHAQTALQGAQSALLAA
EEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REQLASNKVLTDQTTVEKHPNVQRAAANLRSAYLSFARSALPAPVTGYVAKRSVQVGQRV
HHHHHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AAGAPLMAIVPLDQVWVDANFKEVQITHMRIGQPVTLEADVYGSKVKYNGKVAGFSAGTG
HCCCCEEEEEECHHEEECCCCCEEEEEEEECCCCEEEEECCCCCEEEECCEEEEECCCCC
SAFSLLPAQNATGNWIKVVQRLPVRIALDPQQLKDHPLRVGLSMNVTVDVRHEEGAAMVS
CEEEEECCCCCCCHHHHHHHHCCCEEEECHHHHCCCCEEEEEEEEEEEEEEECCCCHHHH
AAAAKPMQTDVYDKVAQDADQLIARIIAVNNGGRAPAPADAVPAKGAPVINVPADDAASA
HHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHH
AVARPSNRG
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]