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The map label for this gene is dapE [H]

Identifier: 73541584

GI number: 73541584

Start: 2077553

End: 2078704

Strand: Direct

Name: dapE [H]

Synonym: Reut_A1895

Alternate gene names: 73541584

Gene position: 2077553-2078704 (Clockwise)

Preceding gene: 73541583

Following gene: 73541585

Centisome position: 54.58

GC content: 66.41

Gene sequence:

>1152_bases
ATGACCGCCACCCTTGCCCTCACCGAAGACCTGATCCGCCGCCGCTCCGTCACGCCCGCGGACGAAGGCTGCCAGGCCGT
GCTGGAAACGCGCCTGAAGGCGCTCGGCTTCACGTGCGAAGCCATTGTCAGCGGCCCCGACGATTTCCGCGTGACCAACC
TGTGGGCCGTGAAGCGCGGCACGCAAGGCACGGACGGCAAGCTGCTGGCCTTTGCCGGTCACACCGACGTGGTGCCGACC
GGCCCGCTCGAACAATGGAACTCGGACCCGTTCGAGCCGACGCACCGTGACGGTCGCCTGTATGGCCGTGGCGCAGCGGA
CATGAAGACGTCGATCGCCGGCTTTGTCGTGGCGGTCGAGGAATTCGTCAAGGCGCATCCGGCCCATGCGGGCTCGATCG
CGTTCCTGATCACCAGCGACGAGGAAGGCCCGGCGCACGACGGTACCATCAAGGTCGTCGAAGCGCTGAAGGCGCGCGGC
GAACGCCTGGACTACTGCGTGATCGGCGAACCCACGTCGGTCGACACGCTCGGCGACATGGTCAAGAACGGCCGCCGTGG
CTCGCTGTCGGGCAAGCTCGTCGTCAAGGGCGTGCAGTGCCATATCGCCTACCCGCACCTGGGCCGCAACCCGATCCACG
AAGCCGCGCCCGCGCTGGCCGAGCTGGCTGCCGAGGTATGGGACCAGGGCAACGAGTACTTCCCGCCGACGAGCTGGCAG
ATGTCGAACATCCACGGCGGCACCGGCGCGACCAACGTCATCCCGGGCCACGTCACGATCGACTTCAACTTCCGCTTCTC
GACCGCAAGCACGCCCGAAGGCCTCAAGTCCCGCGTGCATGCGATCCTGGACAAGCACAAGCTCGAATACACGCTGGACT
GGACGCTCGGCGGCGAGCCGTTCCTGACCCCGCGCGGCGACCTGTCCGATGCGCTGTCGGCCGCCATCGAGGCCGAGACC
GGCGTCAAGACCGAACTGTCGACCACGGGCGGCACATCCGATGGCCGCTTCATCGCCAAGATCTGCCCGCAAGTGATCGA
GTTCGGGCCGCCCAACGCGACCATCCACAAGATCGACGAGAACGTCGAAGTCCGCTTTATCGATCCGCTCAAGAACGTCT
ATCGCGGCGTGCTCGAACGGCTCATTGCCTGA

Upstream 100 bases:

>100_bases
GTCGCTGATCAAGCGCCCGGTGCTCGCGCACAACGGCAAGGTCTCCGTGGGTTTCTCCGCCGACCAGTACGCCAGCCAGT
TCTGAATTTTTTGTCCTCCC

Downstream 100 bases:

>100_bases
TCGCCTGCCGATCGCTCCCCCCATCGACCACAGGACTCCACGACCATGGCAACCCCCTCCCCGCTGCGCACCGTGCGCGA
CCTGCTGCGCCTGGCGGTGT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTATLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCEAIVSGPDDFRVTNLWAVKRGTQGTDGKLLAFAGHTDVVPT
GPLEQWNSDPFEPTHRDGRLYGRGAADMKTSIAGFVVAVEEFVKAHPAHAGSIAFLITSDEEGPAHDGTIKVVEALKARG
ERLDYCVIGEPTSVDTLGDMVKNGRRGSLSGKLVVKGVQCHIAYPHLGRNPIHEAAPALAELAAEVWDQGNEYFPPTSWQ
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPEGLKSRVHAILDKHKLEYTLDWTLGGEPFLTPRGDLSDALSAAIEAET
GVKTELSTTGGTSDGRFIAKICPQVIEFGPPNATIHKIDENVEVRFIDPLKNVYRGVLERLIA

Sequences:

>Translated_383_residues
MTATLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCEAIVSGPDDFRVTNLWAVKRGTQGTDGKLLAFAGHTDVVPT
GPLEQWNSDPFEPTHRDGRLYGRGAADMKTSIAGFVVAVEEFVKAHPAHAGSIAFLITSDEEGPAHDGTIKVVEALKARG
ERLDYCVIGEPTSVDTLGDMVKNGRRGSLSGKLVVKGVQCHIAYPHLGRNPIHEAAPALAELAAEVWDQGNEYFPPTSWQ
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPEGLKSRVHAILDKHKLEYTLDWTLGGEPFLTPRGDLSDALSAAIEAET
GVKTELSTTGGTSDGRFIAKICPQVIEFGPPNATIHKIDENVEVRFIDPLKNVYRGVLERLIA
>Mature_382_residues
TATLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCEAIVSGPDDFRVTNLWAVKRGTQGTDGKLLAFAGHTDVVPTG
PLEQWNSDPFEPTHRDGRLYGRGAADMKTSIAGFVVAVEEFVKAHPAHAGSIAFLITSDEEGPAHDGTIKVVEALKARGE
RLDYCVIGEPTSVDTLGDMVKNGRRGSLSGKLVVKGVQCHIAYPHLGRNPIHEAAPALAELAAEVWDQGNEYFPPTSWQM
SNIHGGTGATNVIPGHVTIDFNFRFSTASTPEGLKSRVHAILDKHKLEYTLDWTLGGEPFLTPRGDLSDALSAAIEAETG
VKTELSTTGGTSDGRFIAKICPQVIEFGPPNATIHKIDENVEVRFIDPLKNVYRGVLERLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=381, Percent_Identity=58.005249343832, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1790395, Length=364, Percent_Identity=26.6483516483516, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789236, Length=224, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41313; Mature: 41182

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCEAIVSGPDDFRVTNLWAVKRG
CCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEECC
TQGTDGKLLAFAGHTDVVPTGPLEQWNSDPFEPTHRDGRLYGRGAADMKTSIAGFVVAVE
CCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
EFVKAHPAHAGSIAFLITSDEEGPAHDGTIKVVEALKARGERLDYCVIGEPTSVDTLGDM
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
VKNGRRGSLSGKLVVKGVQCHIAYPHLGRNPIHEAAPALAELAAEVWDQGNEYFPPTSWQ
HHCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPEGLKSRVHAILDKHKLEYTLDWTLGGEP
ECCCCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
FLTPRGDLSDALSAAIEAETGVKTELSTTGGTSDGRFIAKICPQVIEFGPPNATIHKIDE
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEECC
NVEVRFIDPLKNVYRGVLERLIA
CCEEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCEAIVSGPDDFRVTNLWAVKRG
CEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEEEEEECC
TQGTDGKLLAFAGHTDVVPTGPLEQWNSDPFEPTHRDGRLYGRGAADMKTSIAGFVVAVE
CCCCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
EFVKAHPAHAGSIAFLITSDEEGPAHDGTIKVVEALKARGERLDYCVIGEPTSVDTLGDM
HHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHH
VKNGRRGSLSGKLVVKGVQCHIAYPHLGRNPIHEAAPALAELAAEVWDQGNEYFPPTSWQ
HHCCCCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
MSNIHGGTGATNVIPGHVTIDFNFRFSTASTPEGLKSRVHAILDKHKLEYTLDWTLGGEP
ECCCCCCCCCCCEECCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCC
FLTPRGDLSDALSAAIEAETGVKTELSTTGGTSDGRFIAKICPQVIEFGPPNATIHKIDE
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHCCCCCCEEEEECC
NVEVRFIDPLKNVYRGVLERLIA
CCEEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA