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The map label for this gene is ansB [H]

Identifier: 73541501

GI number: 73541501

Start: 1982276

End: 1983400

Strand: Direct

Name: ansB [H]

Synonym: Reut_A1812

Alternate gene names: 73541501

Gene position: 1982276-1983400 (Clockwise)

Preceding gene: 73541500

Following gene: 73541502

Centisome position: 52.08

GC content: 63.91

Gene sequence:

>1125_bases
ATGAAACGTTTCGCCCGCACCACGACTCTGGCCGCCGCCCTGCTCGCCGCCAGCCTGATCACCGGTACTGCCCACGCTCA
GCTTGCCCCGGCCGCTCCGTCATCGGGAACGCCCGCTGGGACCCAGGCGGAAGCCCGCAAGGCCAATATCGTCATCATCG
GCACGGGCGGCACCATTGCCGGCGCCGGCGCATCGGCGACCAACACGGCGGCCTACCAGTCCGCGGTGGTGCCGGTCGAC
AAGATCATTGCCTCGGTGCCAGAGATCTCGAAGGTCGCGAACGTCAAGGGCGAGCAGATTTTCCAGATCGGTTCGGAGAG
CTTCAACAACGAGCGCCTGCTGAAACTGGGCAAGCGTGTTTCGGAACTGCTCAAGCAGCCCGACGTTGATGGCATTGTCA
TCACGCATGGCACCGACACCATCGAGGAAACGGCCTACTTCCTGAACCTGACGCTCAAGAGCGACAAGCCCGTAGTGGTG
GTTGGCTCGATGCGTCCCGGTACGGCGCTCGGTGCCGATGGCGCGCTGAACCTGTATGACGCCGTGCTGGTTGCTTCCAA
TCCCGCGTCCCGCGGCAAGGGCACGCTGGCGGTGCTGAACGACGAAATCCACACCGGCCGCGATGTGACCAAGAGCAATA
CCTTCAAGACCGAAACCTTCCGCTCGCCGTTTGGCCCGCTCGGCTACGTCGTGGAAGGCCGCACGCTGTTCTACCGGCTG
CCGGCTCGCCCGCACACCACGCAGACCGAGTTCGACATCGACAAGATCGACAAGCTTCCTGAAGTCGCGGTGGTCTATGC
CTATGGCAACGTGAACCCCGGCAGCGTCGACGCTGCTGTGAAGAACGGCGCCAAGGCGATCATCTATGCCGCCACCGGCA
ACGGCAGCGTCGGCGACTACATGGTCGAGCCGTTGAAGGCGGCGCGCGCTAAGGGCGTGCAGATCGTGCGTGCCTCGCGC
ACCGGCAGCGGTGTGGTGATCCGCAACGCCGAGCAACCGGACGACAAGTACGACTGGATCGTCACCGACGACCAGCTTCC
GCAGAAGGCGCGCATCCTGATGGCGCTGGCGCTGACCAAGACCAACGACAGCAAGCAGCTGCAGCAGGTCATGTGGAAGT
ACTGA

Upstream 100 bases:

>100_bases
ACCATGGCCCGAAACCCGGGCCTCCTCGCAGCCACCAATCGAACATAAGCGGCGGCGTCCGGCCCAAAAGGCACGCAAGC
CGTTTGCACCGAGGAGAATC

Downstream 100 bases:

>100_bases
GCCTTCGGGGCTCTCCCTCTTTGTGCTGGTAGTAAGTTGAGCGCGCCGGATTCCGGCGCGCTTTTTTGTTTGAGGCGGAA
CCTGTCGGTTAATTGCGTTA

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKRFARTTTLAAALLAASLITGTAHAQLAPAAPSSGTPAGTQAEARKANIVIIGTGGTIAGAGASATNTAAYQSAVVPVD
KIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVV
VGSMRPGTALGADGALNLYDAVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRTLFYRL
PARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNGSVGDYMVEPLKAARAKGVQIVRASR
TGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMALALTKTNDSKQLQQVMWKY

Sequences:

>Translated_374_residues
MKRFARTTTLAAALLAASLITGTAHAQLAPAAPSSGTPAGTQAEARKANIVIIGTGGTIAGAGASATNTAAYQSAVVPVD
KIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVV
VGSMRPGTALGADGALNLYDAVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRTLFYRL
PARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNGSVGDYMVEPLKAARAKGVQIVRASR
TGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMALALTKTNDSKQLQQVMWKY
>Mature_374_residues
MKRFARTTTLAAALLAASLITGTAHAQLAPAAPSSGTPAGTQAEARKANIVIIGTGGTIAGAGASATNTAAYQSAVVPVD
KIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVV
VGSMRPGTALGADGALNLYDAVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRTLFYRL
PARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNGSVGDYMVEPLKAARAKGVQIVRASR
TGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMALALTKTNDSKQLQQVMWKY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=327, Percent_Identity=46.177370030581, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788065, Length=205, Percent_Identity=31.219512195122, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=352, Percent_Identity=40.0568181818182, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323187, Length=352, Percent_Identity=40.0568181818182, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323186, Length=352, Percent_Identity=40.0568181818182, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6323184, Length=352, Percent_Identity=40.0568181818182, Blast_Score=215, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6320527, Length=327, Percent_Identity=37.3088685015291, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24645530, Length=207, Percent_Identity=28.5024154589372, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.38 [H]

Molecular weight: Translated: 39566; Mature: 39566

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFARTTTLAAALLAASLITGTAHAQLAPAAPSSGTPAGTQAEARKANIVIIGTGGTIA
CCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEE
GAGASATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRV
CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHCCCCHHEEEECCCCCCCHHHHHHHHHH
SELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLYD
HHHHHCCCCCEEEEECCCCCHHCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEE
AVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRTLFYRL
EEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEECHHHCCCCCCCCEEEECCEEEEEC
PARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNGSVGDY
CCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEECCCCCCCHH
MVEPLKAARAKGVQIVRASRTGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMALALTK
HHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHEEEEEEEEC
TNDSKQLQQVMWKY
CCCHHHHHHHHHCC
>Mature Secondary Structure
MKRFARTTTLAAALLAASLITGTAHAQLAPAAPSSGTPAGTQAEARKANIVIIGTGGTIA
CCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEE
GAGASATNTAAYQSAVVPVDKIIASVPEISKVANVKGEQIFQIGSESFNNERLLKLGKRV
CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHCCCCHHEEEECCCCCCCHHHHHHHHHH
SELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLYD
HHHHHCCCCCEEEEECCCCCHHCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEE
AVLVASNPASRGKGTLAVLNDEIHTGRDVTKSNTFKTETFRSPFGPLGYVVEGRTLFYRL
EEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEECHHHCCCCCCCCEEEECCEEEEEC
PARPHTTQTEFDIDKIDKLPEVAVVYAYGNVNPGSVDAAVKNGAKAIIYAATGNGSVGDY
CCCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCEEEEEEECCCCCCCHH
MVEPLKAARAKGVQIVRASRTGSGVVIRNAEQPDDKYDWIVTDDQLPQKARILMALALTK
HHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCCCHHHHEEEEEEEEC
TNDSKQLQQVMWKY
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]