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The map label for this gene is mii [H]

Identifier: 73541500

GI number: 73541500

Start: 1980778

End: 1981971

Strand: Direct

Name: mii [H]

Synonym: Reut_A1811

Alternate gene names: 73541500

Gene position: 1980778-1981971 (Clockwise)

Preceding gene: 161611260

Following gene: 73541501

Centisome position: 52.04

GC content: 66.58

Gene sequence:

>1194_bases
ATGGCCCACGTACCCCAGATCAAGATTCCCGCCACCTACATTCGCGGTGGCACCAGCAAGGGCGTGTTCTTCCGCCTGCA
GGACCTGCCCGAGACGGCGCAGGTTCCCGGCAAGGCACGCGACGCGCTGCTGATGCGCGTGATTGGCAGCCCTGACCCGT
ACGGCAAGCAGATCGACGGCATGGGGGCTGCGACGTCGAGCACCAGCAAGACGGTGATCGTGTCGAAGAGCAGCCGCCCG
GACCACGACGTCGACTACCTGTTCGGCCAGGTCTCGATCGACCAGCCGTTCGTCGACTGGAGCGGCAACTGCGGCAACCT
GTCCGCCGCAGTCGGCCCGTTCGCGATCAGCGGCGGCCTCGTCGATGCGTCGCGCGTGCCGCAGAATGGCACGGCCGTCG
TGCGCATCTGGCAGGCGAACATCGGCAAGACCATCATCGCCCACGTGCCCATCACCAACGGTGAAGTGCAGGAAACCGGC
GATTTCGAACTCGACGGCGTGACCTTCCCCGCCGCTGAAGTCCAGCTCGAATTCATGGACCCGGCCGCCGACGAAGAAGG
CGACGGCGGCGGTGCGATGTTCCCGACGGGCAATCTCGTCGATGATCTCGAAGTGCCTGGCGTGGGCACATTCAAGGCGA
CCATGATCAACGCCGGCATTCCGACGATCTTCGTCAACGCGGAAGCCATCGGCTACACCGGCACCGAACTGCAGGAAGCC
ATCAACGGCGATCCGAAGGCGCTGGCGATGTTCGAGACGATCCGTGCCTACGGCGCCGTGCGCATGGGCCTGATCAAGAG
CGTCGATGAGGCCGCCAAGCGCCAGCACACGCCGAAGGTCGCCTTCGTCGCGAAGCCGAAGGACTATGTCGCTTCCAGCG
GCAAGCCGGTCGGCGCCGGCGACGTGGACCTCCTCGTGCGTGCGCTGTCGATGGGCAAGCTGCACCACGCGATGATGGGC
ACGGCCGCCGTGGCGATTGGCACCGCTGCAGCCATCCCGGGTACGCTGGTCAACCTGGCGGCCGGTGGCGGCGAACGCAA
TGCGGTGCGCTTTGGCCATCCGTCGGGCACGCTGCGCGTGGGTGCGGAAGCGAGCCAGTCCAACGGCGACTGGACCGTCA
CCAAGGCCATCATGAGCCGCAGCGCACGCGTGCTGATGGAAGGCTGGGTGCGCGTGCCGGGCGACGCGTTCTGA

Upstream 100 bases:

>100_bases
CTTCCTTGAATCCGCGAAAGCGGCTGCTTAAGGTAGCGAGATAAGCGGGCGGTGCGGCAACGTACCGCCCGCTGCAGTTT
TGACCAGGACAAAGACTTCC

Downstream 100 bases:

>100_bases
TTGGAAAGCGCCGGGGTCCCGGCGCGCTCCTGACCTTGCCGCCGGTGGCAATACTTCCGCCGGCCATCCGGACCGCCGCA
ATGCATATGCGGATTTCTAT

Product: methylitaconate delta2-delta3-isomerase

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAHVPQIKIPATYIRGGTSKGVFFRLQDLPETAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVIVSKSSRP
DHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISGGLVDASRVPQNGTAVVRIWQANIGKTIIAHVPITNGEVQETG
DFELDGVTFPAAEVQLEFMDPAADEEGDGGGAMFPTGNLVDDLEVPGVGTFKATMINAGIPTIFVNAEAIGYTGTELQEA
INGDPKALAMFETIRAYGAVRMGLIKSVDEAAKRQHTPKVAFVAKPKDYVASSGKPVGAGDVDLLVRALSMGKLHHAMMG
TAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEASQSNGDWTVTKAIMSRSARVLMEGWVRVPGDAF

Sequences:

>Translated_397_residues
MAHVPQIKIPATYIRGGTSKGVFFRLQDLPETAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVIVSKSSRP
DHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISGGLVDASRVPQNGTAVVRIWQANIGKTIIAHVPITNGEVQETG
DFELDGVTFPAAEVQLEFMDPAADEEGDGGGAMFPTGNLVDDLEVPGVGTFKATMINAGIPTIFVNAEAIGYTGTELQEA
INGDPKALAMFETIRAYGAVRMGLIKSVDEAAKRQHTPKVAFVAKPKDYVASSGKPVGAGDVDLLVRALSMGKLHHAMMG
TAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEASQSNGDWTVTKAIMSRSARVLMEGWVRVPGDAF
>Mature_396_residues
AHVPQIKIPATYIRGGTSKGVFFRLQDLPETAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVIVSKSSRPD
HDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISGGLVDASRVPQNGTAVVRIWQANIGKTIIAHVPITNGEVQETGD
FELDGVTFPAAEVQLEFMDPAADEEGDGGGAMFPTGNLVDDLEVPGVGTFKATMINAGIPTIFVNAEAIGYTGTELQEAI
NGDPKALAMFETIRAYGAVRMGLIKSVDEAAKRQHTPKVAFVAKPKDYVASSGKPVGAGDVDLLVRALSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRVGAEASQSNGDWTVTKAIMSRSARVLMEGWVRVPGDAF

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=387, Percent_Identity=34.625322997416, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41373; Mature: 41242

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHVPQIKIPATYIRGGTSKGVFFRLQDLPETAQVPGKARDALLMRVIGSPDPYGKQIDG
CCCCCCEECCHHEECCCCCCCEEEEEECCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
MGAATSSTSKTVIVSKSSRPDHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISGGL
CCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCC
VDASRVPQNGTAVVRIWQANIGKTIIAHVPITNGEVQETGDFELDGVTFPAAEVQLEFMD
EEHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEEGDGGGAMFPTGNLVDDLEVPGVGTFKATMINAGIPTIFVNAEAIGYTGTELQEA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCCHHHHHH
INGDPKALAMFETIRAYGAVRMGLIKSVDEAAKRQHTPKVAFVAKPKDYVASSGKPVGAG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCC
DVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEE
GAEASQSNGDWTVTKAIMSRSARVLMEGWVRVPGDAF
CCCCCCCCCCEEEHHHHHHHHHHEEECCCEECCCCCC
>Mature Secondary Structure 
AHVPQIKIPATYIRGGTSKGVFFRLQDLPETAQVPGKARDALLMRVIGSPDPYGKQIDG
CCCCCEECCHHEECCCCCCCEEEEEECCCCHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
MGAATSSTSKTVIVSKSSRPDHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPFAISGGL
CCCCCCCCCEEEEEECCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCC
VDASRVPQNGTAVVRIWQANIGKTIIAHVPITNGEVQETGDFELDGVTFPAAEVQLEFMD
EEHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHEEEEEEEC
PAADEEGDGGGAMFPTGNLVDDLEVPGVGTFKATMINAGIPTIFVNAEAIGYTGTELQEA
CCCCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECEECCCCCHHHHHH
INGDPKALAMFETIRAYGAVRMGLIKSVDEAAKRQHTPKVAFVAKPKDYVASSGKPVGAG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCC
DVDLLVRALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGERNAVRFGHPSGTLRV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCEEE
GAEASQSNGDWTVTKAIMSRSARVLMEGWVRVPGDAF
CCCCCCCCCCEEEHHHHHHHHHHEEECCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA