Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 73541497

GI number: 73541497

Start: 1975879

End: 1976787

Strand: Direct

Name: prpB [H]

Synonym: Reut_A1808

Alternate gene names: 73541497

Gene position: 1975879-1976787 (Clockwise)

Preceding gene: 73541495

Following gene: 73541498

Centisome position: 51.91

GC content: 66.34

Gene sequence:

>909_bases
ATGACCTATTCCGCATCCGACCTGGCCCGCTCCGCTGGCGCCCGCTTCCGCCAGGCCCTGGCCGAAGAGCACCCGCTGCA
GGTGGTGGGCACCATCAACGCCAACCACGCCCTGCTGGCCAAGCGCGCCGGCTACCGTGCGATCTACCTGTCGGGCGGCG
GCGTGGCCGCGGGCTCGCTGGGCCTGCCTGACCTGGGCATCTCGGGCCTGGAAGACGTGCTGATCGACGTGCGCCGCATC
AGCGATGTATGCGATGTGCCGCTGCTGGTCGACGTCGACACCGGCTTTGGTTCGTCGGCCTTCAACGTGGCACGCACCAC
CCGCTCGCTGATCAAGGCCGGCGCCGGTGCCATGCACATCGAAGACCAGGTCGGCGCGAAGCGCTGCGGCCACCGCCCGA
ACAAGGAAATCGTCACGCAGACCGAGATGGTCGACCGCATCAAGGCCGCCGTTGACGCCCGTACCGACGAGAACTTCGTG
ATTATGGCGCGTACCGACGCGCTGGCCGTGGAAGGCCTGGAAAGCGCCATCGAGCGCGCCGTGGCGTGCGTCGAGGCTGG
CGCCGACGCGATCTTCCCGGAAGCCATGACCGACCTGGCCATGTACCGCAAGTTCGTGGACGCCGTGAAGGTGCCCGTGC
TGGCCAACATCACCGAATTTGGCGCCACGCCGCTGTTCACCACCGACGAACTGGCCGGCTCCGGCGTGTCGATGGTGCTG
TACCCGCTGTCGGCCTTCCGCGCGATGAACAAGGCCGCCGAGAACGTCTACACCGCCATCCGCCGCGACGGCACCCAGAA
GAATGTGGTCGAGACCATGCAGACCCGTGCTGAACTGTACGAAAGCATCGGCTACCACGCCTACGAACAGAAACTCGACG
CCCTGTTTGCACAGGGCAAGGCCAAGTAA

Upstream 100 bases:

>100_bases
CAATAGGTTAGTTGCTCACCATGAGTCCGCCGCGCAGGCTGGCATAGCGCTTGCAGTAAGGGCCCTGTTCATTCCCTGCC
TGAAACGAGACACCGCAACC

Downstream 100 bases:

>100_bases
GGCCACCCACCTGTCGACCCGTACCTCACAAGGAGACCCAGATGTCCGAAGCACAAGCGATCGCCACCCCGAAGCCCAAG
AAGTCCGTTGCCCTGTCGGG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTYSASDLARSAGARFRQALAEEHPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLEDVLIDVRRI
SDVCDVPLLVDVDTGFGSSAFNVARTTRSLIKAGAGAMHIEDQVGAKRCGHRPNKEIVTQTEMVDRIKAAVDARTDENFV
IMARTDALAVEGLESAIERAVACVEAGADAIFPEAMTDLAMYRKFVDAVKVPVLANITEFGATPLFTTDELAGSGVSMVL
YPLSAFRAMNKAAENVYTAIRRDGTQKNVVETMQTRAELYESIGYHAYEQKLDALFAQGKAK

Sequences:

>Translated_302_residues
MTYSASDLARSAGARFRQALAEEHPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLEDVLIDVRRI
SDVCDVPLLVDVDTGFGSSAFNVARTTRSLIKAGAGAMHIEDQVGAKRCGHRPNKEIVTQTEMVDRIKAAVDARTDENFV
IMARTDALAVEGLESAIERAVACVEAGADAIFPEAMTDLAMYRKFVDAVKVPVLANITEFGATPLFTTDELAGSGVSMVL
YPLSAFRAMNKAAENVYTAIRRDGTQKNVVETMQTRAELYESIGYHAYEQKLDALFAQGKAK
>Mature_301_residues
TYSASDLARSAGARFRQALAEEHPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLEDVLIDVRRIS
DVCDVPLLVDVDTGFGSSAFNVARTTRSLIKAGAGAMHIEDQVGAKRCGHRPNKEIVTQTEMVDRIKAAVDARTDENFVI
MARTDALAVEGLESAIERAVACVEAGADAIFPEAMTDLAMYRKFVDAVKVPVLANITEFGATPLFTTDELAGSGVSMVLY
PLSAFRAMNKAAENVYTAIRRDGTQKNVVETMQTRAELYESIGYHAYEQKLDALFAQGKAK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=293, Percent_Identity=75.0853242320819, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790445, Length=257, Percent_Identity=26.4591439688716, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32304; Mature: 32173

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSASDLARSAGARFRQALAEEHPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSL
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEECCC
GLPDLGISGLEDVLIDVRRISDVCDVPLLVDVDTGFGSSAFNVARTTRSLIKAGAGAMHI
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
EDQVGAKRCGHRPNKEIVTQTEMVDRIKAAVDARTDENFVIMARTDALAVEGLESAIERA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
VACVEAGADAIFPEAMTDLAMYRKFVDAVKVPVLANITEFGATPLFTTDELAGSGVSMVL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCHHHHH
YPLSAFRAMNKAAENVYTAIRRDGTQKNVVETMQTRAELYESIGYHAYEQKLDALFAQGK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
AK
CC
>Mature Secondary Structure 
TYSASDLARSAGARFRQALAEEHPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSL
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCEEEEEECCCEEECCC
GLPDLGISGLEDVLIDVRRISDVCDVPLLVDVDTGFGSSAFNVARTTRSLIKAGAGAMHI
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEE
EDQVGAKRCGHRPNKEIVTQTEMVDRIKAAVDARTDENFVIMARTDALAVEGLESAIERA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
VACVEAGADAIFPEAMTDLAMYRKFVDAVKVPVLANITEFGATPLFTTDELAGSGVSMVL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCHHHHH
YPLSAFRAMNKAAENVYTAIRRDGTQKNVVETMQTRAELYESIGYHAYEQKLDALFAQGK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]