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The map label for this gene is catB [H]

Identifier: 73541378

GI number: 73541378

Start: 1823294

End: 1824418

Strand: Direct

Name: catB [H]

Synonym: Reut_A1689

Alternate gene names: 73541378

Gene position: 1823294-1824418 (Clockwise)

Preceding gene: 73541376

Following gene: 73541379

Centisome position: 47.9

GC content: 66.31

Gene sequence:

>1125_bases
ATGACAGCCACGATTACCTCGGTCGAAGCCATCCTGGTCGACCTGCCCACCATCCGGGCCCATCAACTGGCCATGGCCAC
CATGCAGCAGCAGACGCTGGTGATCGTGCGGCTGCGCTGCAGCGACGGCATCGAAGGCATCGGCGAGGCCACCACCATCG
GCGGCCTTTCCTATGGCGATGAAAGCCCCGAAGGCATCAAGCTGGCGATCGACACTTACCTGGCGCCTGCGCTGGCCGGC
GAAGATGCGACCAATGTCCATGCCGCCATGGCACGCCTGAACAAGATCGCGCGCGGCAATCGCTTTGCCAAGTCGGCGCT
GGAGACAGCGCTGCTTGATGCGCAGGGCAAGCGCCTCGGTGTGCCGCTCGCCACGCTGCTCGGCGGCGCCGTGCGTACCA
CGCTGCCGGTGCTGTGGACGTTGGCCAGCGGCGATACCGCGAGGGACATCGACGAAGCCGAGCGCCTGCTGGCCGAGCGC
CGGCACAACACGTTCAAGCTCAAGATCGGCCGGCGCAGCGTGCGTGATGACGTGGCGCACGTGTCCGCGATCAAGCGCGC
GCTGGGTGACCGTGCACGCGTGACAGTGGATATCAACCAGGCGTGGAACGAGGCCGATGCCGCGGGTGGTATTGCCATGC
TCGAAGCCGCTGGCATCGACCTGATCGAGCAACCCACGCCGCGTGAGCAACGTACGGCGCTGGCCCGTCTGGCGGCGCGC
TTCGTCGTGCCGATCATGGCGGATGAAGCGGTATGTGGCCCAGAAGATGCCATGGAACTCGCGCGCATCGGCGGTGCCGA
TGTGTTCGCGCTGAAGATCGCGAAATCGGGCGGCATCTTCGGCATGCTGCGCACGGCAGCGGTTGGCGATGCCGCGGGTA
TCGCGCTATACGGCGGTACGATGCTGGAAGGCAGCGTGGGCACCATCGCTGCCGCGCACGGCTTCTGCACGCTGCCGCAA
CTTGCGTGGGGCACAGAGTTGTTCGGCCCGCTGCTGCTCAAGGACGATGTAGTCGTGCAGCGGCCCGAATATCGCGATTT
CAGCCTGCACCTGCCCGCAGGCCCGGGACTGGGCCTCGCGCTCGATGAAGACAAGCTCGCGCACTACCGGCGCGACCGCG
CTTAA

Upstream 100 bases:

>100_bases
GGCATACCTGTAGGGTATTGATTGAGACATCATCGGTCTTGGACGTGACTCGCGTCAAGATTCATACTTCGTCGACAATC
CTTCTCCGCAGCGCAAAGGT

Downstream 100 bases:

>100_bases
AGAACTGATCTGATTAGCCAAGAGGAACGCCATGCTTTACCTGGTCCGGATGGATGTGAACCTGCCGCACGACATGCCTG
CTGCGCAGGCCGATGACATC

Product: muconate cycloisomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MTATITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAG
EDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRTTLPVLWTLASGDTARDIDEAERLLAER
RHNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRDRA

Sequences:

>Translated_374_residues
MTATITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAG
EDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRTTLPVLWTLASGDTARDIDEAERLLAER
RHNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQ
LAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRDRA
>Mature_373_residues
TATITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLAIDTYLAPALAGE
DATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRTTLPVLWTLASGDTARDIDEAERLLAERR
HNTFKLKIGRRSVRDDVAHVSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAARF
VVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQL
AWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLALDEDKLAHYRRDRA

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=323, Percent_Identity=24.1486068111455, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081876, Length=295, Percent_Identity=27.1186440677966, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 39611; Mature: 39480

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEECCCCCCC
ESPEGIKLAIDTYLAPALAGEDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLG
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPLATLLGGAVRTTLPVLWTLASGDTARDIDEAERLLAERRHNTFKLKIGRRSVRDDVAH
CCHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
VSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHCCCCEEEEEEHHHCCCCHHHCCCEEEEHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLA
EECCCCCCEEHHCCCCCCCHHHHCHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCCCEE
LDEDKLAHYRRDRA
ECHHHHHHHHHCCC
>Mature Secondary Structure 
TATITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGD
CCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEEECCCCCCC
ESPEGIKLAIDTYLAPALAGEDATNVHAAMARLNKIARGNRFAKSALETALLDAQGKRLG
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
VPLATLLGGAVRTTLPVLWTLASGDTARDIDEAERLLAERRHNTFKLKIGRRSVRDDVAH
CCHHHHHCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHH
VSAIKRALGDRARVTVDINQAWNEADAAGGIAMLEAAGIDLIEQPTPREQRTALARLAAR
HHHHHHHCCCCEEEEEEHHHCCCCHHHCCCEEEEHHCCCHHHCCCCCHHHHHHHHHHHHH
FVVPIMADEAVCGPEDAMELARIGGADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGT
HHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCE
MLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDVVVQRPEYRDFSLHLPAGPGLGLA
EECCCCCCEEHHCCCCCCCHHHHCHHHHCCCCCCCCEEEECCCCCCEEEECCCCCCCCEE
LDEDKLAHYRRDRA
ECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]