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The map label for this gene is leuA [H]

Identifier: 73541376

GI number: 73541376

Start: 1820616

End: 1822283

Strand: Direct

Name: leuA [H]

Synonym: Reut_A1687

Alternate gene names: 73541376

Gene position: 1820616-1822283 (Clockwise)

Preceding gene: 73541375

Following gene: 73541378

Centisome position: 47.83

GC content: 63.97

Gene sequence:

>1668_bases
ATGATTGCCCAGCCCTCCGCCAAATACCAACCGTTCCCGCCCGTCGGCCTCACCGATCGCACCTGGCCGAACCGCACGAT
TACCTCAGCGCCCATGTGGCTTTCGACCGACCTGAGGGACGGCAACCAGGCGTTGTTCGAGCCGATGAACGGCGAGCGCA
AGATGCGTCTGTTCCAGGAACTCGTGCGCATCGGCTTCAAGGAAATCGAAGTCGGCTTCCCGTCGGCGTCGCAGACCGAT
TTCGACTTCATCCGCAAGCTGATCGATGAAAACCTGATTCCCGACGACGTGACCATCATGGTCATCACGCAGTCGCGCGA
GGAGCTGATCGACCGCACCGTGGAAGCACTGAAGGGTGCGAGCAAGGCTATCGTCCACGTCTACAACGCGGTTGCGCCGG
CCTGGCGCCGAATCGTGTTCGGCATGAGCGTGCCCGAAGTGATGTCGCTCGTGCACCATCACATCGACTACCTGAAGACG
CTGACCGACCAGCATCCTGAAACCAAGTGGACGTTGCAGTATTCGCCCGAGACCTTCAGCGCAGCCGAGCTCGAGGTGTC
GCTGCAGGCGTGCCATACGGCCATCGAGGCATGGGGTGCAAGTGCCGACCGCCGCGTGATCATCAACCTGCCGACCACCG
TCGAAAACGCGACGCCTAACGTATTTGCCGACCAGATCGAATGGATGCACCGCCGTCTCGTGCCGCGCAAGCATATCGTG
CTGTCCGTGCATCCGCATAACGACCGCGGCACCGGCGTGGCTGCCGCCGAACTCGCGATGATGGCTGGCGCGGACCGCGT
CGAAGGCTGCCTGTTCGGAAACGGCGAGCGCTGCGGCAACGTGGACATCGTCACGCTCGCCATGAACATGTACACGCAGG
GCGTGCATCCGAACCTCGATTTCTCCGACATCAACGCCGTGGCCCGGATTGCCGAAGAAGCGACGTCGATCCCCGTGCAT
CCGCGCCACCCCTATGCGGGCGACCTCGTGTTCACGGCGTTCTCGGGCTCGCACCAGGATGCGATCAAGAAGGGCTTTGC
CGCACAGGATCCGAACGGCGTGTGGGAAGTGCCTTACCTGCCCATCGACCCGGCCGACCTCGGCCGCACCTATGACAGCG
TGATCCGCGTGAACAGCCAGTCGGGCAAGGGTGGTATTGCGTTCATGCTCGAACGCGAGCACGGCGTGGTGATGCCGCGC
CGCATGCAGGTGGAATTCAGCGCGGTCGTGCAGCGGGCTACCGACGCGAGCGAGACCGAGATCAGCGGCCCGGAACTCTG
GAAGCTGTTCGGGGAGACGTATCTCCCATCCGATGCATCGGGCCAGGGCATCGTCTACCACGCCTACAAGCTCGACGAGA
GCGGGGAGGGCATTGCGCTCGACGTGACGGTCGACGGCGTGCGCAAGACGCTGCACGGCAAGGGTAATGGCCCCATCGCA
GCCGCAGTCCACGCGCTCGACCTGCGCATGCGCATTGATGCCTACGAAGAGCGCGCCACCGGCGCCGGTGCCGATGCACA
GGCGATGGCCATCGTCGAAGCCGCGCTGGAAGGCGTGCCCGGCGCCAGCTTCGGTGTCGGCATGAGCCACAACATCGTGG
AAGCGTCAGTGCAGGCTGTGATCAGCGTAGCGAACCGCCTGGTGCGCCAGCGCCGCTCACAGGCCTGA

Upstream 100 bases:

>100_bases
GCCATGCGAGGGGCTGCAGGGGAAGTCACATTGCATTTGCCTGTTTGACTTCCCGGCACGGCCCGCAGCGGACCGTCCGG
CACCGGATTCGTGGACCATG

Downstream 100 bases:

>100_bases
TTCCCCCGATTCTGGACCAGTCAATCCCTACTGCCCTTGACGCATGACCCAGGCTACCGCCTGGGCCAGGAACTGAGAGC
TGTCACCAACCCGATAGCTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MIAQPSAKYQPFPPVGLTDRTWPNRTITSAPMWLSTDLRDGNQALFEPMNGERKMRLFQELVRIGFKEIEVGFPSASQTD
FDFIRKLIDENLIPDDVTIMVITQSREELIDRTVEALKGASKAIVHVYNAVAPAWRRIVFGMSVPEVMSLVHHHIDYLKT
LTDQHPETKWTLQYSPETFSAAELEVSLQACHTAIEAWGASADRRVIINLPTTVENATPNVFADQIEWMHRRLVPRKHIV
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLAMNMYTQGVHPNLDFSDINAVARIAEEATSIPVH
PRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNGVWEVPYLPIDPADLGRTYDSVIRVNSQSGKGGIAFMLEREHGVVMPR
RMQVEFSAVVQRATDASETEISGPELWKLFGETYLPSDASGQGIVYHAYKLDESGEGIALDVTVDGVRKTLHGKGNGPIA
AAVHALDLRMRIDAYEERATGAGADAQAMAIVEAALEGVPGASFGVGMSHNIVEASVQAVISVANRLVRQRRSQA

Sequences:

>Translated_555_residues
MIAQPSAKYQPFPPVGLTDRTWPNRTITSAPMWLSTDLRDGNQALFEPMNGERKMRLFQELVRIGFKEIEVGFPSASQTD
FDFIRKLIDENLIPDDVTIMVITQSREELIDRTVEALKGASKAIVHVYNAVAPAWRRIVFGMSVPEVMSLVHHHIDYLKT
LTDQHPETKWTLQYSPETFSAAELEVSLQACHTAIEAWGASADRRVIINLPTTVENATPNVFADQIEWMHRRLVPRKHIV
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLAMNMYTQGVHPNLDFSDINAVARIAEEATSIPVH
PRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNGVWEVPYLPIDPADLGRTYDSVIRVNSQSGKGGIAFMLEREHGVVMPR
RMQVEFSAVVQRATDASETEISGPELWKLFGETYLPSDASGQGIVYHAYKLDESGEGIALDVTVDGVRKTLHGKGNGPIA
AAVHALDLRMRIDAYEERATGAGADAQAMAIVEAALEGVPGASFGVGMSHNIVEASVQAVISVANRLVRQRRSQA
>Mature_555_residues
MIAQPSAKYQPFPPVGLTDRTWPNRTITSAPMWLSTDLRDGNQALFEPMNGERKMRLFQELVRIGFKEIEVGFPSASQTD
FDFIRKLIDENLIPDDVTIMVITQSREELIDRTVEALKGASKAIVHVYNAVAPAWRRIVFGMSVPEVMSLVHHHIDYLKT
LTDQHPETKWTLQYSPETFSAAELEVSLQACHTAIEAWGASADRRVIINLPTTVENATPNVFADQIEWMHRRLVPRKHIV
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLAMNMYTQGVHPNLDFSDINAVARIAEEATSIPVH
PRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNGVWEVPYLPIDPADLGRTYDSVIRVNSQSGKGGIAFMLEREHGVVMPR
RMQVEFSAVVQRATDASETEISGPELWKLFGETYLPSDASGQGIVYHAYKLDESGEGIALDVTVDGVRKTLHGKGNGPIA
AAVHALDLRMRIDAYEERATGAGADAQAMAIVEAALEGVPGASFGVGMSHNIVEASVQAVISVANRLVRQRRSQA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=549, Percent_Identity=23.6794171220401, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=47.6190476190476, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324682, Length=568, Percent_Identity=46.3028169014084, Blast_Score=523, Evalue=1e-149,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60846; Mature: 60846

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQPSAKYQPFPPVGLTDRTWPNRTITSAPMWLSTDLRDGNQALFEPMNGERKMRLFQE
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEECCCCCCCHHHHCCCCCHHHHHHHHH
LVRIGFKEIEVGFPSASQTDFDFIRKLIDENLIPDDVTIMVITQSREELIDRTVEALKGA
HHHCCHHEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHCC
SKAIVHVYNAVAPAWRRIVFGMSVPEVMSLVHHHIDYLKTLTDQHPETKWTLQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
AAELEVSLQACHTAIEAWGASADRRVIINLPTTVENATPNVFADQIEWMHRRLVPRKHIV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLAMNMYTQGVHPNLD
EEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDINAVARIAEEATSIPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNGVWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCEEECCCC
PIDPADLGRTYDSVIRVNSQSGKGGIAFMLEREHGVVMPRRMQVEFSAVVQRATDASETE
CCCHHHHCCCHHHHEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ISGPELWKLFGETYLPSDASGQGIVYHAYKLDESGEGIALDVTVDGVRKTLHGKGNGPIA
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCCCHH
AAVHALDLRMRIDAYEERATGAGADAQAMAIVEAALEGVPGASFGVGMSHNIVEASVQAV
HHHHHHHHEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ISVANRLVRQRRSQA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIAQPSAKYQPFPPVGLTDRTWPNRTITSAPMWLSTDLRDGNQALFEPMNGERKMRLFQE
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEECCCCCCCHHHHCCCCCHHHHHHHHH
LVRIGFKEIEVGFPSASQTDFDFIRKLIDENLIPDDVTIMVITQSREELIDRTVEALKGA
HHHCCHHEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHCC
SKAIVHVYNAVAPAWRRIVFGMSVPEVMSLVHHHIDYLKTLTDQHPETKWTLQYSPETFS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
AAELEVSLQACHTAIEAWGASADRRVIINLPTTVENATPNVFADQIEWMHRRLVPRKHIV
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
LSVHPHNDRGTGVAAAELAMMAGADRVEGCLFGNGERCGNVDIVTLAMNMYTQGVHPNLD
EEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEHHHHCCCCCCCC
FSDINAVARIAEEATSIPVHPRHPYAGDLVFTAFSGSHQDAIKKGFAAQDPNGVWEVPYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHCCCCCCCCCCEEECCCC
PIDPADLGRTYDSVIRVNSQSGKGGIAFMLEREHGVVMPRRMQVEFSAVVQRATDASETE
CCCHHHHCCCHHHHEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
ISGPELWKLFGETYLPSDASGQGIVYHAYKLDESGEGIALDVTVDGVRKTLHGKGNGPIA
CCCHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCCCHH
AAVHALDLRMRIDAYEERATGAGADAQAMAIVEAALEGVPGASFGVGMSHNIVEASVQAV
HHHHHHHHEEEEHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ISVANRLVRQRRSQA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA