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The map label for this gene is gatA2 [H]

Identifier: 73541348

GI number: 73541348

Start: 1788282

End: 1789805

Strand: Direct

Name: gatA2 [H]

Synonym: Reut_A1658

Alternate gene names: 73541348

Gene position: 1788282-1789805 (Clockwise)

Preceding gene: 73541347

Following gene: 73541353

Centisome position: 46.98

GC content: 66.14

Gene sequence:

>1524_bases
ATGTACTCGAAGGACTCGCTGACTTCCGCCTCCGCAGTCGAACTTCGCCGGCTGATCGGCACCCGCGAAATCTCTCCCGT
GGAGCTGCTGGACGCCTGCATTGCCCGTATCGAGACCTACAACCCAGCCATCAACGCGATCACGGCGACTTGCTACGAGC
GGGCGCGCACGGAGGCGAGGGCGGCCGAGCAGGCCGTCATGCGCGGCGAGCCGCTTGGCCTGTTGCACGGGCTGCCGCTG
GGCGTCAAGGATCTGGAGGCGACCGCCGGGCTGCTCACGACTTATGGCTCGCCGCTTTACCGTGACAACGTGCCCAGCCA
GGACAATGTCCTTGTGGCACGCCTGCGCGCGGCCGGCGCCATCGTGGTTGGCAAGACCAATATTCCGGAGATGGGTGCGG
GGGCAAACTCCCGCAATGCGGTATGGGGCGCCACCGGCAACCCGTTCAATCCGAACCTGAACGCCGGGGGCTCCTCGGGC
GGCTCGGCCGCGGCGCTGGCCGCAGGCTTTCTGCCGGTCTGTACGGGGTCCGATACGGGAGGATCGCTGCGTATCCCGGC
GGCCAAGTGCGGCGTGGTGGGCTTCCGGCCTTCGCCGGGCGTCGTGCCGAACTCGCGCAAGCTGTTGGGCTGGACCCCGA
TTTCGGTGGTTGGCCCGATGGGCCGCACGGTCGCCGACGCCTGCCTGCAGCTGGCAGCATCGGCGGGTGCATCGGCCTCC
GATCCGCTCAGCTATGAACTCGACCCTGGCGCCTTCCTGACCCCGCAGCACGTCGACCTTTCCGACCTGCGTGTCGGCTG
GACAGAGGATTTTGGAAGTTGCAGCGTCGACGACGGCATCCGCCGCGTCTTTCGCGCGAAGATCGCCGAAATGCGGCACC
TCTTCAAGTCCTGCGATGAGGTCAGTTTCGATCTGGGCGATGTGCACCGCTGCTTTGACGTGTTGCGCGCGGAGAGCTTC
GTCGCGAGCCTGCATGAGGCCTATCAGCGCGATCCCGGCAGCCTCGGGCCCAACACCCGCGCCAACTATGAGCTGGGCGC
GAAGATGTCGCTCGGCGACAGCGCCTGGGCCCAGGCCGAACAGACACGGATCCTGCAACGCTTCCAGCAGGCATACCGGG
ACTATGACGTCATCCTGTCGCCGACGACGCCGGTCTCGCCATTTCCGTGGACCCGCCTGTTCGCGGAAATCATCAATGGC
GAGCGGCAAGAGAACTATTACCGCTGGCTGGCACTGACCTATGTGGTCACCCTGACCACACACCCGGCCATTTCATTGCC
ATGCGGCGTGGACGAGCAAAGCATGCCATTCGGTCTGCAGATAACCGGAGGCTTTCGCGGCGATGCCATGGCGCTCGGCG
TGGCGCAAGCGATGGAGACCGCCTTTGCGGCCTCGCCAGCACTGTGCCGTCCGATTCCCGACCTCGATGCCATCGTGCCG
GCCGTGCCCGCGCTTCGCTCCATCGTGACGGCGCCGCCCACCTTTGGCGGGACGACGCAGTCGAACGGCGTATCGGCGGT
TTGA

Upstream 100 bases:

>100_bases
CGCTCCAATATCAAGCTCGACTGAGCCTGCGCAAACGCCCACCGATCCACGTCCCGGGCCGCTCGCAAACCCAGCGGCCG
CTCCAGGAAACTATCGTCCC

Downstream 100 bases:

>100_bases
ATCCGCGTCCCGCTACCAGTTGAGCTCAGGATCGGCTCGCCGAGGCGGCAGCGTGCCGCCCGGCTTGTCGGCCGAAGAAG
GTGGCATCCGCGAGTGACAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MYSKDSLTSASAVELRRLIGTREISPVELLDACIARIETYNPAINAITATCYERARTEARAAEQAVMRGEPLGLLHGLPL
GVKDLEATAGLLTTYGSPLYRDNVPSQDNVLVARLRAAGAIVVGKTNIPEMGAGANSRNAVWGATGNPFNPNLNAGGSSG
GSAAALAAGFLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPNSRKLLGWTPISVVGPMGRTVADACLQLAASAGASAS
DPLSYELDPGAFLTPQHVDLSDLRVGWTEDFGSCSVDDGIRRVFRAKIAEMRHLFKSCDEVSFDLGDVHRCFDVLRAESF
VASLHEAYQRDPGSLGPNTRANYELGAKMSLGDSAWAQAEQTRILQRFQQAYRDYDVILSPTTPVSPFPWTRLFAEIING
ERQENYYRWLALTYVVTLTTHPAISLPCGVDEQSMPFGLQITGGFRGDAMALGVAQAMETAFAASPALCRPIPDLDAIVP
AVPALRSIVTAPPTFGGTTQSNGVSAV

Sequences:

>Translated_507_residues
MYSKDSLTSASAVELRRLIGTREISPVELLDACIARIETYNPAINAITATCYERARTEARAAEQAVMRGEPLGLLHGLPL
GVKDLEATAGLLTTYGSPLYRDNVPSQDNVLVARLRAAGAIVVGKTNIPEMGAGANSRNAVWGATGNPFNPNLNAGGSSG
GSAAALAAGFLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPNSRKLLGWTPISVVGPMGRTVADACLQLAASAGASAS
DPLSYELDPGAFLTPQHVDLSDLRVGWTEDFGSCSVDDGIRRVFRAKIAEMRHLFKSCDEVSFDLGDVHRCFDVLRAESF
VASLHEAYQRDPGSLGPNTRANYELGAKMSLGDSAWAQAEQTRILQRFQQAYRDYDVILSPTTPVSPFPWTRLFAEIING
ERQENYYRWLALTYVVTLTTHPAISLPCGVDEQSMPFGLQITGGFRGDAMALGVAQAMETAFAASPALCRPIPDLDAIVP
AVPALRSIVTAPPTFGGTTQSNGVSAV
>Mature_507_residues
MYSKDSLTSASAVELRRLIGTREISPVELLDACIARIETYNPAINAITATCYERARTEARAAEQAVMRGEPLGLLHGLPL
GVKDLEATAGLLTTYGSPLYRDNVPSQDNVLVARLRAAGAIVVGKTNIPEMGAGANSRNAVWGATGNPFNPNLNAGGSSG
GSAAALAAGFLPVCTGSDTGGSLRIPAAKCGVVGFRPSPGVVPNSRKLLGWTPISVVGPMGRTVADACLQLAASAGASAS
DPLSYELDPGAFLTPQHVDLSDLRVGWTEDFGSCSVDDGIRRVFRAKIAEMRHLFKSCDEVSFDLGDVHRCFDVLRAESF
VASLHEAYQRDPGSLGPNTRANYELGAKMSLGDSAWAQAEQTRILQRFQQAYRDYDVILSPTTPVSPFPWTRLFAEIING
ERQENYYRWLALTYVVTLTTHPAISLPCGVDEQSMPFGLQITGGFRGDAMALGVAQAMETAFAASPALCRPIPDLDAIVP
AVPALRSIVTAPPTFGGTTQSNGVSAV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=511, Percent_Identity=28.1800391389432, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI222831590, Length=486, Percent_Identity=28.1893004115226, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI166795287, Length=211, Percent_Identity=29.8578199052133, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=516, Percent_Identity=25.968992248062, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17543272, Length=475, Percent_Identity=25.6842105263158, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=310, Percent_Identity=30.3225806451613, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=293, Percent_Identity=27.9863481228669, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=195, Percent_Identity=29.2307692307692, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=194, Percent_Identity=32.4742268041237, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=186, Percent_Identity=33.8709677419355, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=467, Percent_Identity=25.2676659528908, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=430, Percent_Identity=22.3255813953488, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=28.2608695652174, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356731, Length=494, Percent_Identity=26.3157894736842, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=28.3168316831683, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=28.3168316831683, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=28.3168316831683, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=28.3168316831683, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45550774, Length=507, Percent_Identity=25.2465483234714, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648435, Length=507, Percent_Identity=25.2465483234714, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648437, Length=507, Percent_Identity=25.2465483234714, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24644968, Length=503, Percent_Identity=26.441351888668, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648113, Length=460, Percent_Identity=27.1739130434783, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078093, Length=528, Percent_Identity=26.8939393939394, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=23.7327188940092, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=23.7327188940092, Blast_Score=89, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53674; Mature: 53674

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSKDSLTSASAVELRRLIGTREISPVELLDACIARIETYNPAINAITATCYERARTEAR
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAEQAVMRGEPLGLLHGLPLGVKDLEATAGLLTTYGSPLYRDNVPSQDNVLVARLRAAGA
HHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEHHHCCE
IVVGKTNIPEMGAGANSRNAVWGATGNPFNPNLNAGGSSGGSAAALAAGFLPVCTGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCC
GSLRIPAAKCGVVGFRPSPGVVPNSRKLLGWTPISVVGPMGRTVADACLQLAASAGASAS
CEEECCHHHCCEEECCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCC
DPLSYELDPGAFLTPQHVDLSDLRVGWTEDFGSCSVDDGIRRVFRAKIAEMRHLFKSCDE
CCCEEEECCCCCCCCCCCCHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSFDLGDVHRCFDVLRAESFVASLHEAYQRDPGSLGPNTRANYELGAKMSLGDSAWAQAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
QTRILQRFQQAYRDYDVILSPTTPVSPFPWTRLFAEIINGERQENYYRWLALTYVVTLTT
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
HPAISLPCGVDEQSMPFGLQITGGFRGDAMALGVAQAMETAFAASPALCRPIPDLDAIVP
CCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHH
AVPALRSIVTAPPTFGGTTQSNGVSAV
HHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYSKDSLTSASAVELRRLIGTREISPVELLDACIARIETYNPAINAITATCYERARTEAR
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AAEQAVMRGEPLGLLHGLPLGVKDLEATAGLLTTYGSPLYRDNVPSQDNVLVARLRAAGA
HHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEHHHCCE
IVVGKTNIPEMGAGANSRNAVWGATGNPFNPNLNAGGSSGGSAAALAAGFLPVCTGSDTG
EEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCC
GSLRIPAAKCGVVGFRPSPGVVPNSRKLLGWTPISVVGPMGRTVADACLQLAASAGASAS
CEEECCHHHCCEEECCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCC
DPLSYELDPGAFLTPQHVDLSDLRVGWTEDFGSCSVDDGIRRVFRAKIAEMRHLFKSCDE
CCCEEEECCCCCCCCCCCCHHHCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VSFDLGDVHRCFDVLRAESFVASLHEAYQRDPGSLGPNTRANYELGAKMSLGDSAWAQAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHH
QTRILQRFQQAYRDYDVILSPTTPVSPFPWTRLFAEIINGERQENYYRWLALTYVVTLTT
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEC
HPAISLPCGVDEQSMPFGLQITGGFRGDAMALGVAQAMETAFAASPALCRPIPDLDAIVP
CCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHH
AVPALRSIVTAPPTFGGTTQSNGVSAV
HHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]