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The map label for this gene is xthA [H]

Identifier: 73540991

GI number: 73540991

Start: 1386529

End: 1387356

Strand: Reverse

Name: xthA [H]

Synonym: Reut_A1297

Alternate gene names: 73540991

Gene position: 1387356-1386529 (Counterclockwise)

Preceding gene: 73540992

Following gene: 73540990

Centisome position: 36.45

GC content: 65.1

Gene sequence:

>828_bases
ATGAGCGCGGTGCTGCCTACGTCGGGGCCGCTTCGCATTGCGAGCTGGAACGTCAACTCCCTCAAGGTCCGCCTGCCGCA
GGTGCTGCAATGGCTGACGGAACAGGAACAGGCCGGCGCGCCGATCGACGCGCTGTGCCTGCAGGAACTGAAGCTGCCTG
ACGACAAGTATCCGCTTGCGGAACTTGAGAACGCCGGCTTCCACAGCGTGTACACGGGCCAGAAGACGTACAACGGCGTC
GCCATCCTTGCGCGCGATGCCAGCATGCCCGGTCCGGTCGACGTGGTGCGCAACATCCCGGGCTTCGAAGATGCGCAGCA
GCGCGTCGTGGCAGCCACGTATGGCGACATCCGCCTGGTTTGCGCGTATTTCCCGAACGGGCAGGCGCCGGATTCCGACA
AGTTCGTCTACAAGCTCAAGTGGCTCGACGCCATGACGGCATGGCTGCGCGAAGAGCTCGCGAAGTATCCGCGGCTCGCG
CTGCTCGGCGATTTCAATATCGCCCCGGAAGACCGCGACGTGCACGATCCGGCCAAGTGGGAAGGCCAGAATCTGGTCTC
GCCGCAGGAGCGCGCAGCGTTCACCGCGCTCGTCGAACTCGGTCTGGCCGATGCGTTCCGCAAGTTCGAACAGCCTGAAA
AAGCGTTCTCGTGGTGGGACTACCGCATGTTCGCCTTCCGGCGCAATGCCGGCCTGCGCATCGACCACATCCTGCTGTCG
CCGGAACTGGCCAGCAGCTGCTCGGCATGCGTGATCGACCGCGCGCCGCGCACCTGGGAGCAGCCGTCCGATCACACGCC
GGTGGTCGCCACGCTGAACTGCGGCTGA

Upstream 100 bases:

>100_bases
TGTCGGTCGGCGGCAGCCGCCCGGCCATGGATTCGTTCGTCGCGTTCCGCGGCCGCGCGCCGCAGATCGACGCGCTGCTG
CGCCACGGAGGCATGGCAGC

Downstream 100 bases:

>100_bases
ACCGCGTGCTCCGTTCACGCAAGTCCGGGCAAGCCCGGCTCGCGGCGAACGGGGTGCGCCGCTACACTGCGGCGATGACC
CCCGCATCACCCCGCCATCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSAVLPTSGPLRIASWNVNSLKVRLPQVLQWLTEQEQAGAPIDALCLQELKLPDDKYPLAELENAGFHSVYTGQKTYNGV
AILARDASMPGPVDVVRNIPGFEDAQQRVVAATYGDIRLVCAYFPNGQAPDSDKFVYKLKWLDAMTAWLREELAKYPRLA
LLGDFNIAPEDRDVHDPAKWEGQNLVSPQERAAFTALVELGLADAFRKFEQPEKAFSWWDYRMFAFRRNAGLRIDHILLS
PELASSCSACVIDRAPRTWEQPSDHTPVVATLNCG

Sequences:

>Translated_275_residues
MSAVLPTSGPLRIASWNVNSLKVRLPQVLQWLTEQEQAGAPIDALCLQELKLPDDKYPLAELENAGFHSVYTGQKTYNGV
AILARDASMPGPVDVVRNIPGFEDAQQRVVAATYGDIRLVCAYFPNGQAPDSDKFVYKLKWLDAMTAWLREELAKYPRLA
LLGDFNIAPEDRDVHDPAKWEGQNLVSPQERAAFTALVELGLADAFRKFEQPEKAFSWWDYRMFAFRRNAGLRIDHILLS
PELASSCSACVIDRAPRTWEQPSDHTPVVATLNCG
>Mature_274_residues
SAVLPTSGPLRIASWNVNSLKVRLPQVLQWLTEQEQAGAPIDALCLQELKLPDDKYPLAELENAGFHSVYTGQKTYNGVA
ILARDASMPGPVDVVRNIPGFEDAQQRVVAATYGDIRLVCAYFPNGQAPDSDKFVYKLKWLDAMTAWLREELAKYPRLAL
LGDFNIAPEDRDVHDPAKWEGQNLVSPQERAAFTALVELGLADAFRKFEQPEKAFSWWDYRMFAFRRNAGLRIDHILLSP
ELASSCSACVIDRAPRTWEQPSDHTPVVATLNCG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=277, Percent_Identity=32.4909747292419, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375503, Length=277, Percent_Identity=32.4909747292419, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI18375501, Length=277, Percent_Identity=32.4909747292419, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788046, Length=275, Percent_Identity=36, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=200, Percent_Identity=27.5, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=28.3582089552239, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30759; Mature: 30628

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVLPTSGPLRIASWNVNSLKVRLPQVLQWLTEQEQAGAPIDALCLQELKLPDDKYPLA
CCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
ELENAGFHSVYTGQKTYNGVAILARDASMPGPVDVVRNIPGFEDAQQRVVAATYGDIRLV
HHCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCHHHHEEEEEECCCEEEE
CAYFPNGQAPDSDKFVYKLKWLDAMTAWLREELAKYPRLALLGDFNIAPEDRDVHDPAKW
EEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC
EGQNLVSPQERAAFTALVELGLADAFRKFEQPEKAFSWWDYRMFAFRRNAGLRIDHILLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCCEEEEEEEC
PELASSCSACVIDRAPRTWEQPSDHTPVVATLNCG
CHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SAVLPTSGPLRIASWNVNSLKVRLPQVLQWLTEQEQAGAPIDALCLQELKLPDDKYPLA
CCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHH
ELENAGFHSVYTGQKTYNGVAILARDASMPGPVDVVRNIPGFEDAQQRVVAATYGDIRLV
HHCCCCCCEEECCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCHHHHEEEEEECCCEEEE
CAYFPNGQAPDSDKFVYKLKWLDAMTAWLREELAKYPRLALLGDFNIAPEDRDVHDPAKW
EEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC
EGQNLVSPQERAAFTALVELGLADAFRKFEQPEKAFSWWDYRMFAFRRNAGLRIDHILLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCCEEEEEEEC
PELASSCSACVIDRAPRTWEQPSDHTPVVATLNCG
CHHHHCCCEEEECCCCCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]