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The map label for this gene is relA [H]

Identifier: 73540969

GI number: 73540969

Start: 1358327

End: 1360552

Strand: Direct

Name: relA [H]

Synonym: Reut_A1274

Alternate gene names: 73540969

Gene position: 1358327-1360552 (Clockwise)

Preceding gene: 73540968

Following gene: 73540970

Centisome position: 35.68

GC content: 65.59

Gene sequence:

>2226_bases
ATGGTGACGTCCACCGACTTGGCCGGCCAGGTGGCCGGCGTCCCGGACGCCGACATGGTCGAGCGCGCGCTCGCGTACGT
ACGCGAGCAGGCGGGCGACGCCGTGCTGCCGACTGGCGAGCCGGTGCTGTCGCATGCGGAAGGAATGCTGCGCATCCTCG
ACGGTCTGCGCGTGGATGATGCCGCGCGCGCCGCGTGCTGCCTGTTCCCGCTGGCCGAATTCGCGCCCGCCGTAGAGCCG
GAAATCGAGGCGCGCTTTGGCGAAGATGTATGCCGGATGGTGAAGGGCGTACGGCAACTGCTGCGCATCGGCGCCATTGC
CGGCGGGCACGACGCGCAGGAGGCATCGCGCAACGTGGCGCAGGCCCGGCACGAGCAGGTCGAAGCGCTGCGCAAGATGC
TGCTGGCGTTCGCGCAAGATATCCGCGTGGTGCTGGTGCGCCTGGCTTCGCGGCTGCAGTCGCTGCGCTGGCTCGCGCAG
AACAAGCGTGAACCGCTACCCGGCATCGCGCGCGAGACGCTCGACATCTATGCGCCGCTGGCTAACCGCCTCGGCATCTG
GCAGATGAAGTGGGAACTCGAGGACCTGGCCTTCCGCTTCGAGCAGCCTGATACCTACAAGCGCATCGCAAAGTTGCTCG
ATGAAAAGCGCATCGAGCGCGAGGGCTACATCGCCAGCGCCATCGCGCGCCTGCAATCGGAGCTGGCCGACGCCGGCATC
CGCGCCGAGGTAAGCGGGCGGCCCAAGCACATCTACAGCATCTGGAAGAAGATGCGCGGCAAGGAGCTGGATTTTGCCGA
CCTGTACGATGTGCGCGCGTTCCGCGTGATCGTCGACGACATCAAGGACTGCTATACGGTCCTCGGTATCGTCCACCATA
TCTGGCAGCCGATTCCACGCGAGTTCGACGACTACATTTCGCGGCCCAAATCGAACGGCTACAAGTCGCTGCATACGGTG
GTGATCGGGGACGACGGGCGTGCGTTCGAGGTACAGATCCGCACGCACGAAATGCACCATTTCGCCGAGTACGGCGTGGC
GGCGCACTGGCGCTACAAAGAAGCGGGCAGCAAGGGCTACAGCGGACAGTTCGCGGCAAGCGACAGCTACGACGAGAAGA
TCGCGTGGCTGCGGCAACTGCTGGCTTGGAAGGATGATGCCGACCACAGCGTGGCGCATGACGAGTCGCCGTCGCCGTGG
GAGCAGCTCAAGCACGCGGCGATCGACGACCACATCTACGTGCTGACGCCGCAGGCACGCGTGATCGCGCTGCCGCAAGG
TGCCACGCCGCTCGACTTCGCGTATTACCTGCACAGTGATCTTGGCCACCGGTGCCGCGGCGCGCGTGTCGACGGCACGA
TGGTGCCACTGAATACGCCACTGAAGAACGGTCAGACCGTCGAGATCATCGCCGTGAAGCAGGGCGGGCCGTCGCGCGAC
TGGCTCAACATCGAACTCGGCTACCTGGCCAGCAGCCGCGCGCGGGCCAAGGTACGGGCGTGGTTCAATGCGCTGGATTC
GCAGGAGACTATTGCGCAAGGCCGCGTGCTGATCGACAAGACGCTGCAGCGCGAAGGCAAGACCGCCGTCAAGCTCGAAG
ACCTCGCCACGCGGCTCGGCTTCAAGTCGCCGGACGACCTCTTCGCCGCAGTGGCGAAGGAGGAGTTCAGCCTGCGGCAT
GTCGAGCAAGCGCTGCGGCACCCCGAGGGCGAGGTGCAGGCGCCGTTCAACGAGGAAGACGCCGTCACCAAGAAGAGCCG
CGCCACCAGCGTGGCGCGCGGCGCCAAATCCGGCGTGCTTGTGGTCGGCGTGGATTCGTTGATGACGCAGATGTCGCGCT
GCTGCAAGCCGGCACCTCCGGACGAGATCGTCGGCTTCGTCACGCGCGGGCGCGGCGTGTCGATCCATCGGCGCAACTGC
CACACGTTCCAGCAACTCTCGGCGCGCGCCCCCGAGCGCGTGATCCAGACCGAGTGGGGCAAGAAGAGCGATGCCGTGTA
TCCGGTCGACATCCACGTCGAGGCCATCGACCGCCAGGGGCTGCTGCGTGATATCTCCGAAGTGCTGTCGCGCGAGAAGA
TCAACGTGACCGGCGTGAAGACGCTGTCGAGCAAGGGCGTGGCGCGCATGCAGTTCACAGCCGAGGTGTCGGAGGCCACG
CAACTGCAGCGCGCGCTGTCGATCATCGAAGATGTGCAGGGGGTATTGCAAGCACGAAGAAAGTGA

Upstream 100 bases:

>100_bases
GCGCCACCGTCGAAGCCAGGCTGGCCAATCCCGACTACCTGATCGAAGTGGTGGTGACCGCTGCGCTCAACTGAAGATGG
CGTTGTGACATGGTGACCCT

Downstream 100 bases:

>100_bases
GGCTATAATTGCTGCTTCGCTAGGCTCGTAGCTCAGCTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGA
GTCCAATCGAGCCTACCAAC

Product: RelA/SpoT protein

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MVTSTDLAGQVAGVPDADMVERALAYVREQAGDAVLPTGEPVLSHAEGMLRILDGLRVDDAARAACCLFPLAEFAPAVEP
EIEARFGEDVCRMVKGVRQLLRIGAIAGGHDAQEASRNVAQARHEQVEALRKMLLAFAQDIRVVLVRLASRLQSLRWLAQ
NKREPLPGIARETLDIYAPLANRLGIWQMKWELEDLAFRFEQPDTYKRIAKLLDEKRIEREGYIASAIARLQSELADAGI
RAEVSGRPKHIYSIWKKMRGKELDFADLYDVRAFRVIVDDIKDCYTVLGIVHHIWQPIPREFDDYISRPKSNGYKSLHTV
VIGDDGRAFEVQIRTHEMHHFAEYGVAAHWRYKEAGSKGYSGQFAASDSYDEKIAWLRQLLAWKDDADHSVAHDESPSPW
EQLKHAAIDDHIYVLTPQARVIALPQGATPLDFAYYLHSDLGHRCRGARVDGTMVPLNTPLKNGQTVEIIAVKQGGPSRD
WLNIELGYLASSRARAKVRAWFNALDSQETIAQGRVLIDKTLQREGKTAVKLEDLATRLGFKSPDDLFAAVAKEEFSLRH
VEQALRHPEGEVQAPFNEEDAVTKKSRATSVARGAKSGVLVVGVDSLMTQMSRCCKPAPPDEIVGFVTRGRGVSIHRRNC
HTFQQLSARAPERVIQTEWGKKSDAVYPVDIHVEAIDRQGLLRDISEVLSREKINVTGVKTLSSKGVARMQFTAEVSEAT
QLQRALSIIEDVQGVLQARRK

Sequences:

>Translated_741_residues
MVTSTDLAGQVAGVPDADMVERALAYVREQAGDAVLPTGEPVLSHAEGMLRILDGLRVDDAARAACCLFPLAEFAPAVEP
EIEARFGEDVCRMVKGVRQLLRIGAIAGGHDAQEASRNVAQARHEQVEALRKMLLAFAQDIRVVLVRLASRLQSLRWLAQ
NKREPLPGIARETLDIYAPLANRLGIWQMKWELEDLAFRFEQPDTYKRIAKLLDEKRIEREGYIASAIARLQSELADAGI
RAEVSGRPKHIYSIWKKMRGKELDFADLYDVRAFRVIVDDIKDCYTVLGIVHHIWQPIPREFDDYISRPKSNGYKSLHTV
VIGDDGRAFEVQIRTHEMHHFAEYGVAAHWRYKEAGSKGYSGQFAASDSYDEKIAWLRQLLAWKDDADHSVAHDESPSPW
EQLKHAAIDDHIYVLTPQARVIALPQGATPLDFAYYLHSDLGHRCRGARVDGTMVPLNTPLKNGQTVEIIAVKQGGPSRD
WLNIELGYLASSRARAKVRAWFNALDSQETIAQGRVLIDKTLQREGKTAVKLEDLATRLGFKSPDDLFAAVAKEEFSLRH
VEQALRHPEGEVQAPFNEEDAVTKKSRATSVARGAKSGVLVVGVDSLMTQMSRCCKPAPPDEIVGFVTRGRGVSIHRRNC
HTFQQLSARAPERVIQTEWGKKSDAVYPVDIHVEAIDRQGLLRDISEVLSREKINVTGVKTLSSKGVARMQFTAEVSEAT
QLQRALSIIEDVQGVLQARRK
>Mature_741_residues
MVTSTDLAGQVAGVPDADMVERALAYVREQAGDAVLPTGEPVLSHAEGMLRILDGLRVDDAARAACCLFPLAEFAPAVEP
EIEARFGEDVCRMVKGVRQLLRIGAIAGGHDAQEASRNVAQARHEQVEALRKMLLAFAQDIRVVLVRLASRLQSLRWLAQ
NKREPLPGIARETLDIYAPLANRLGIWQMKWELEDLAFRFEQPDTYKRIAKLLDEKRIEREGYIASAIARLQSELADAGI
RAEVSGRPKHIYSIWKKMRGKELDFADLYDVRAFRVIVDDIKDCYTVLGIVHHIWQPIPREFDDYISRPKSNGYKSLHTV
VIGDDGRAFEVQIRTHEMHHFAEYGVAAHWRYKEAGSKGYSGQFAASDSYDEKIAWLRQLLAWKDDADHSVAHDESPSPW
EQLKHAAIDDHIYVLTPQARVIALPQGATPLDFAYYLHSDLGHRCRGARVDGTMVPLNTPLKNGQTVEIIAVKQGGPSRD
WLNIELGYLASSRARAKVRAWFNALDSQETIAQGRVLIDKTLQREGKTAVKLEDLATRLGFKSPDDLFAAVAKEEFSLRH
VEQALRHPEGEVQAPFNEEDAVTKKSRATSVARGAKSGVLVVGVDSLMTQMSRCCKPAPPDEIVGFVTRGRGVSIHRRNC
HTFQQLSARAPERVIQTEWGKKSDAVYPVDIHVEAIDRQGLLRDISEVLSREKINVTGVKTLSSKGVARMQFTAEVSEAT
QLQRALSIIEDVQGVLQARRK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=717, Percent_Identity=41.7015341701534, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=34.9295774647887, Blast_Score=409, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 82806; Mature: 82806

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSTDLAGQVAGVPDADMVERALAYVREQAGDAVLPTGEPVLSHAEGMLRILDGLRVDD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCH
AARAACCLFPLAEFAPAVEPEIEARFGEDVCRMVKGVRQLLRIGAIAGGHDAQEASRNVA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QARHEQVEALRKMLLAFAQDIRVVLVRLASRLQSLRWLAQNKREPLPGIARETLDIYAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ANRLGIWQMKWELEDLAFRFEQPDTYKRIAKLLDEKRIEREGYIASAIARLQSELADAGI
HHCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RAEVSGRPKHIYSIWKKMRGKELDFADLYDVRAFRVIVDDIKDCYTVLGIVHHIWQPIPR
EEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EFDDYISRPKSNGYKSLHTVVIGDDGRAFEVQIRTHEMHHFAEYGVAAHWRYKEAGSKGY
HHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCHHEEEEHHCCCCCC
SGQFAASDSYDEKIAWLRQLLAWKDDADHSVAHDESPSPWEQLKHAAIDDHIYVLTPQAR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCE
VIALPQGATPLDFAYYLHSDLGHRCRGARVDGTMVPLNTPLKNGQTVEIIAVKQGGPSRD
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCC
WLNIELGYLASSRARAKVRAWFNALDSQETIAQGRVLIDKTLQREGKTAVKLEDLATRLG
EEEEEECCHHCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHCCCCEEHHHHHHHHCC
FKSPDDLFAAVAKEEFSLRHVEQALRHPEGEVQAPFNEEDAVTKKSRATSVARGAKSGVL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VVGVDSLMTQMSRCCKPAPPDEIVGFVTRGRGVSIHRRNCHTFQQLSARAPERVIQTEWG
EEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCHHHHHHHCCC
KKSDAVYPVDIHVEAIDRQGLLRDISEVLSREKINVTGVKTLSSKGVARMQFTAEVSEAT
CCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCCCEEEEEHHHHHHHH
QLQRALSIIEDVQGVLQARRK
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTSTDLAGQVAGVPDADMVERALAYVREQAGDAVLPTGEPVLSHAEGMLRILDGLRVDD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCCCH
AARAACCLFPLAEFAPAVEPEIEARFGEDVCRMVKGVRQLLRIGAIAGGHDAQEASRNVA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QARHEQVEALRKMLLAFAQDIRVVLVRLASRLQSLRWLAQNKREPLPGIARETLDIYAPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ANRLGIWQMKWELEDLAFRFEQPDTYKRIAKLLDEKRIEREGYIASAIARLQSELADAGI
HHCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
RAEVSGRPKHIYSIWKKMRGKELDFADLYDVRAFRVIVDDIKDCYTVLGIVHHIWQPIPR
EEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EFDDYISRPKSNGYKSLHTVVIGDDGRAFEVQIRTHEMHHFAEYGVAAHWRYKEAGSKGY
HHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHHHHHHHCCHHEEEEHHCCCCCC
SGQFAASDSYDEKIAWLRQLLAWKDDADHSVAHDESPSPWEQLKHAAIDDHIYVLTPQAR
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCE
VIALPQGATPLDFAYYLHSDLGHRCRGARVDGTMVPLNTPLKNGQTVEIIAVKQGGPSRD
EEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCC
WLNIELGYLASSRARAKVRAWFNALDSQETIAQGRVLIDKTLQREGKTAVKLEDLATRLG
EEEEEECCHHCCHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHCCCCEEHHHHHHHHCC
FKSPDDLFAAVAKEEFSLRHVEQALRHPEGEVQAPFNEEDAVTKKSRATSVARGAKSGVL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEE
VVGVDSLMTQMSRCCKPAPPDEIVGFVTRGRGVSIHRRNCHTFQQLSARAPERVIQTEWG
EEEHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCHHHHHHHCCC
KKSDAVYPVDIHVEAIDRQGLLRDISEVLSREKINVTGVKTLSSKGVARMQFTAEVSEAT
CCCCCEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCCCEEEEEHHHHHHHH
QLQRALSIIEDVQGVLQARRK
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928955 [H]