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The map label for this gene is epsM [H]

Identifier: 73540830

GI number: 73540830

Start: 1223138

End: 1223809

Strand: Direct

Name: epsM [H]

Synonym: Reut_A1128

Alternate gene names: 73540830

Gene position: 1223138-1223809 (Clockwise)

Preceding gene: 73540828

Following gene: 73540831

Centisome position: 32.13

GC content: 57.14

Gene sequence:

>672_bases
ATGCGAGCAAAGGCAACCATGAAGCGCATCGTCGTCATCGGGTCAGGCGGACATGCCCGGTCCGTCATCGATGTGGTGGA
GAGGCAGGGCAAGTACCGCATCGTCGGGTTAGTAGACGACACTCCGCGCGAAGTGATGGGCTATCGCGTGATCGGCAATG
AGACCGCCCTCCAGGCGCTGGTCAAGGAATACCGGATCGATGAGGCGCTGATCGCTATTGGCGACAACAACGTGCGCGCG
CAGGTAGCCAAACGAATTGAGGCGCTGTGCGCCGGGATTCAATTTGCCGTAGCCATTCATCCAGCGGCATGCATTGGACG
GGATGCAACGATCGGTACTGGCACCGTTGTCATGGCGGGTGCGGTGGTCAATGCATGCTCGGCTATTGGCCGTCATTGCA
TCATCAACACTGGAGCATGCATCGATCATGACTGCAAGGTTGGCGACTTCGTTAGCCTGGCACCGGGAGTCATCACAGGA
GGCGATTGCAACATTGGTACCTATGCCGCCATTGGGTTGGGCGCCAATCTTATCCATGGCGTCACAATAGGGGATCAAAC
GGTCATCGGGGCGGGAGCTCTCGTCATCAATGACATAGAAAGGCAGTCTGTTGCCTATGGGGTGCCAGCCAAAGTTGTCC
GGTTGCGCGAGCAAGGAGAGCAGTATTTGTAA

Upstream 100 bases:

>100_bases
TTGCCGCAATATCTAAGGACTAGTCTCAGGAAGACGGCCGTAGTGCGGTGGGCCCGGCCTGCGTTGCGGAGGGAGGTGCT
CTTGGTCCGCGATACAAACC

Downstream 100 bases:

>100_bases
TCGTCAGTCCAACATGGCGAAGCCTCTTGCGTAAGGTACTGATCCACTCAGGCATCCCGATAAGTTTACTGGAAGGAGAG
AAGTCCTCCACTGATCATGG

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRAKATMKRIVVIGSGGHARSVIDVVERQGKYRIVGLVDDTPREVMGYRVIGNETALQALVKEYRIDEALIAIGDNNVRA
QVAKRIEALCAGIQFAVAIHPAACIGRDATIGTGTVVMAGAVVNACSAIGRHCIINTGACIDHDCKVGDFVSLAPGVITG
GDCNIGTYAAIGLGANLIHGVTIGDQTVIGAGALVINDIERQSVAYGVPAKVVRLREQGEQYL

Sequences:

>Translated_223_residues
MRAKATMKRIVVIGSGGHARSVIDVVERQGKYRIVGLVDDTPREVMGYRVIGNETALQALVKEYRIDEALIAIGDNNVRA
QVAKRIEALCAGIQFAVAIHPAACIGRDATIGTGTVVMAGAVVNACSAIGRHCIINTGACIDHDCKVGDFVSLAPGVITG
GDCNIGTYAAIGLGANLIHGVTIGDQTVIGAGALVINDIERQSVAYGVPAKVVRLREQGEQYL
>Mature_223_residues
MRAKATMKRIVVIGSGGHARSVIDVVERQGKYRIVGLVDDTPREVMGYRVIGNETALQALVKEYRIDEALIAIGDNNVRA
QVAKRIEALCAGIQFAVAIHPAACIGRDATIGTGTVVMAGAVVNACSAIGRHCIINTGACIDHDCKVGDFVSLAPGVITG
GDCNIGTYAAIGLGANLIHGVTIGDQTVIGAGALVINDIERQSVAYGVPAKVVRLREQGEQYL

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23290; Mature: 23290

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAKATMKRIVVIGSGGHARSVIDVVERQGKYRIVGLVDDTPREVMGYRVIGNETALQAL
CCCHHCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCEEEECCHHHHHHH
VKEYRIDEALIAIGDNNVRAQVAKRIEALCAGIQFAVAIHPAACIGRDATIGTGTVVMAG
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCHHEEEEECCHHHCCCCCCCCCCHHHHHH
AVVNACSAIGRHCIINTGACIDHDCKVGDFVSLAPGVITGGDCNIGTYAAIGLGANLIHG
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHCCCCEEECCCCCCCCEEEECCCCHHHEE
VTIGDQTVIGAGALVINDIERQSVAYGVPAKVVRLREQGEQYL
EEECCCEEEECCEEEEECHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRAKATMKRIVVIGSGGHARSVIDVVERQGKYRIVGLVDDTPREVMGYRVIGNETALQAL
CCCHHCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHCEEEECCHHHHHHH
VKEYRIDEALIAIGDNNVRAQVAKRIEALCAGIQFAVAIHPAACIGRDATIGTGTVVMAG
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCHHEEEEECCHHHCCCCCCCCCCHHHHHH
AVVNACSAIGRHCIINTGACIDHDCKVGDFVSLAPGVITGGDCNIGTYAAIGLGANLIHG
HHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHCCCCEEECCCCCCCCEEEECCCCHHHEE
VTIGDQTVIGAGALVINDIERQSVAYGVPAKVVRLREQGEQYL
EEECCCEEEECCEEEEECHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]