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The map label for this gene is rotA [H]

Identifier: 73540822

GI number: 73540822

Start: 1212424

End: 1213011

Strand: Reverse

Name: rotA [H]

Synonym: Reut_A1120

Alternate gene names: 73540822

Gene position: 1213011-1212424 (Counterclockwise)

Preceding gene: 73540823

Following gene: 73540821

Centisome position: 31.87

GC content: 61.56

Gene sequence:

>588_bases
ATGATCCGTTCCCGCCGCATCCTCCTGGCCGGCCTGAGCGCCGCCGCGCTGGCGCTGTCCGCATTGCCGTCGATGGCACA
GCAGACGCAGAAAGCCGAACGTGTGCAGTTCGTCACCAACCAAGGCAAGTTCACCGTCGAGGTCTATCCCGACGCGGCGC
CCAAGACCGTCGCCAACTTCATGGAATATGTGAAGAGCGGCTTCTACAGCGGCACCATCTTCCACCGCGTGATCAACGGC
TTCATGGTCCAGGGCGGGGGCTTCGACCGCGAGATGAAGGAAAAGCCGACGCGTGCTCCGATCCCGCTCGAAGCGCAGAA
CGGCCTGAAGAACAAGGCTGGCACCGTGGCCATGGCCCGCACCGCAAACCCGAATTCCGCCACCGCTCAGTTCTTCGTCA
ATGTGGTGGATAATCCGAATCTCGACTACCCGCAGCCGGACGGCAACGGCTACGCAGTATTCGGCAAGGTGGTGGAAGGC
ATGGATACGATCGAGAAGATCAAGAACGTGCCGACCACGGCCTACGGCCCCATGCGCAATGTGCCGGCTGCGCCCATTGT
GATCGAGTCGGCCAGCGTCATTCAATAA

Upstream 100 bases:

>100_bases
CAGTGCTCCCGCCGCGCGCTGATACCCGCCACCAGACATTGCCTGTTCCGCTGCCGGGCTGCCAAGGCCCGGCAATCACC
AATCTACCGGAGCTTCAGTC

Downstream 100 bases:

>100_bases
ACACCACCGTACAGAGGAAAGCCCATGTCCAAGGTCCAGCTCCAAACCAACCAGGGTGTCATCACCATCGAACTCGACGC
CGAGAAGGCTCCGAAGTCGG

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MIRSRRILLAGLSAAALALSALPSMAQQTQKAERVQFVTNQGKFTVEVYPDAAPKTVANFMEYVKSGFYSGTIFHRVING
FMVQGGGFDREMKEKPTRAPIPLEAQNGLKNKAGTVAMARTANPNSATAQFFVNVVDNPNLDYPQPDGNGYAVFGKVVEG
MDTIEKIKNVPTTAYGPMRNVPAAPIVIESASVIQ

Sequences:

>Translated_195_residues
MIRSRRILLAGLSAAALALSALPSMAQQTQKAERVQFVTNQGKFTVEVYPDAAPKTVANFMEYVKSGFYSGTIFHRVING
FMVQGGGFDREMKEKPTRAPIPLEAQNGLKNKAGTVAMARTANPNSATAQFFVNVVDNPNLDYPQPDGNGYAVFGKVVEG
MDTIEKIKNVPTTAYGPMRNVPAAPIVIESASVIQ
>Mature_195_residues
MIRSRRILLAGLSAAALALSALPSMAQQTQKAERVQFVTNQGKFTVEVYPDAAPKTVANFMEYVKSGFYSGTIFHRVING
FMVQGGGFDREMKEKPTRAPIPLEAQNGLKNKAGTVAMARTANPNSATAQFFVNVVDNPNLDYPQPDGNGYAVFGKVVEG
MDTIEKIKNVPTTAYGPMRNVPAAPIVIESASVIQ

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=161, Percent_Identity=32.9192546583851, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI5454154, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI24308049, Length=160, Percent_Identity=32.5, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI22547212, Length=171, Percent_Identity=30.4093567251462, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI7657473, Length=171, Percent_Identity=30.4093567251462, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI22547215, Length=184, Percent_Identity=30.4347826086957, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI7706339, Length=168, Percent_Identity=29.7619047619048, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1789763, Length=181, Percent_Identity=53.5911602209945, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1786736, Length=161, Percent_Identity=54.6583850931677, Blast_Score=183, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71980594, Length=148, Percent_Identity=35.8108108108108, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71980590, Length=148, Percent_Identity=35.8108108108108, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17539496, Length=159, Percent_Identity=32.0754716981132, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565860, Length=156, Percent_Identity=31.4102564102564, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532641, Length=163, Percent_Identity=30.6748466257669, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559074, Length=156, Percent_Identity=31.4102564102564, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=134, Percent_Identity=35.0746268656716, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17566266, Length=149, Percent_Identity=32.8859060402685, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17506311, Length=147, Percent_Identity=30.6122448979592, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323562, Length=161, Percent_Identity=35.4037267080745, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320359, Length=135, Percent_Identity=34.0740740740741, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922376, Length=179, Percent_Identity=28.4916201117318, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24652460, Length=152, Percent_Identity=30.9210526315789, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922912, Length=165, Percent_Identity=33.9393939393939, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17986117, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45549139, Length=153, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136360, Length=162, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24664125, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357783, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 21040; Mature: 21040

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSRRILLAGLSAAALALSALPSMAQQTQKAERVQFVTNQGKFTVEVYPDAAPKTVANF
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHH
MEYVKSGFYSGTIFHRVINGFMVQGGGFDREMKEKPTRAPIPLEAQNGLKNKAGTVAMAR
HHHHHCCCCCHHHHHHHHHHEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
TANPNSATAQFFVNVVDNPNLDYPQPDGNGYAVFGKVVEGMDTIEKIKNVPTTAYGPMRN
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
VPAAPIVIESASVIQ
CCCCCEEECCCHHCC
>Mature Secondary Structure
MIRSRRILLAGLSAAALALSALPSMAQQTQKAERVQFVTNQGKFTVEVYPDAAPKTVANF
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCHHHHHHH
MEYVKSGFYSGTIFHRVINGFMVQGGGFDREMKEKPTRAPIPLEAQNGLKNKAGTVAMAR
HHHHHCCCCCHHHHHHHHHHEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEE
TANPNSATAQFFVNVVDNPNLDYPQPDGNGYAVFGKVVEGMDTIEKIKNVPTTAYGPMRN
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCC
VPAAPIVIESASVIQ
CCCCCEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]