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The map label for this gene is uvrB

Identifier: 73540754

GI number: 73540754

Start: 1143965

End: 1146052

Strand: Direct

Name: uvrB

Synonym: Reut_A1051

Alternate gene names: 73540754

Gene position: 1143965-1146052 (Clockwise)

Preceding gene: 73540751

Following gene: 73540755

Centisome position: 30.05

GC content: 63.36

Gene sequence:

>2088_bases
ATGTCAAACCTTGCCGAAGTCGCGCCGGCCCTGGACGAAGACAAAATCGTCACTTTTCCGGGCTCGCCGTTCCAGCTCTA
CCAGCCGTTCCCCCCGGCGGGCGACCAGCCGGAGGCTATCCGGCAACTGGTGGAAGGGATAGAGGACGGCTTGTCGTTCC
AGACGCTTCTCGGCGTGACGGGCTCAGGCAAGACCTACACGATGGCCAACGTGATCGCGCGCATGGGGCGCCCGGCCATC
GTCTTTGCACCCAACAAGACGCTGGCTGCCCAGCTTTACTCCGAATTCCGCGAGTTCTTCCCGCGCAATGCCGTCGAGTA
CTTCGTCAGCTACTACGACTACTACCAGCCCGAGGCCTACGTCCCGCAGCGCGACCTGTTTATCGAGAAGGATTCGTCGA
TCAATGAGCACATCGAGCAGATGCGCTTGTCGGCCACCAAGAGCCTGCTGGAGCGCCGCGATACGATCATCGTGGCCACG
GTGTCGGCCATCTACGGTATCGGCAACCCGAACGAATACCACCAGATGATCCTGACGCTGCGTACGGGCGACAAGATCAG
CCAGCGCGACGTGATCGCTCGACTGATTGCCATGCAATACACGCGCAACGAGACCGATTTCCAGCGCGGCACGTTCCGCG
TGCGCGGCGATACCATCGACATTTTCCCGGCCGAACACGCGGAAATGGCGGTGCGGCTCGAGATGTTCGACGACGAAGTG
GAGTCGCTGCAGTTCTTCGACCCGCTCACCGGCCGCGTGCGGCAGAAGATTCCGCGCTTCACCGTTTATCCGTCGAGCCA
CTACGTGACGCCGCGCGAAACTGTCCTTCGCGCGATCGAGGACATCAAGGCGGAGTTGCGCGACCGGCTTGAGTTCTTCC
ACAAGGAAAACCGGCTCGTGGAGGTGCAGCGGCTCGAGCAGCGCACTCGCTTCGACCTGGAAATGCTCTCCGAGCTCGGC
TTCTGCAAGGGCATCGAAAACTATTCGCGCCACCTGTCGGGCGCGAAGCCCGGCGAGCCGCCGCCGACGCTGGTCGATTA
CCTGCCGTCGGACGCGCTGATGTTCCTCGACGAATCGCACGTGCTGATCGGCCAGCTCAACGGCATGTACAACGGCGACC
GCGCGCGCAAGACCACGCTGGTTGAATACGGCTTCCGGCTGCCGTCCGCGCTCGACAACCGGCCGCTCAAGTTCGAGGAG
TTCGAACGCAAGATGCGCCAGGTGATGTTCGTTTCGGCGACACCTGCGCAGTTCGAGAAGGAGCACGCCGGGCAAGTCGT
GGAGCAGGTCGTGCGGCCGACCGGGCTGGTCGACCCGATCATCATCGTGCGGCCCGCTACCACACAGGTCGACGACCTGC
TGTCCGAGATCAACCTGCGCGTGGAGGCCGGCGAACGCGTGCTGGTGACCACGCTCACCAAGCGCATGGCCGAGCAGCTG
ACCGAGTTCCTGTCGGAGAATGGCGTCAAGGTGCGTTACCTGCACTCGGATATCGACACGGTGGAACGCGTGGAAATCAT
CCGCGACCTGCGCCTGGGGACGTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAAGGGCTGGATATCCCCGAGGTGT
CGCTGGTCGCCATCCTCGACGCGGACAAGGAAGGCTTCCTGCGCGCCGAGCGTTCGCTGATCCAGACCATCGGCCGTGCC
GCGCGCAACGTGAACGGGACCGCGATCCTGTACGCCGACCGCATCACCGATTCGATGCGCAAGGCCATCGACGAGACCGA
GCGGCGCCGCGCCAAGCAGATGGCGTTCAACGAGGCCAACGGCATCACGCCGCGCGGCGTGATCAAGCGGATCAAGGACA
TCATCGACGGCGTCTACGATGCGGGCGAGGTCAAGGCCGAACTGCTGGCGGCACAGGAGCGCGCGCGCTACGAGGACATG
AGCGAGAAGCAGGTCTCGAAGGAGATCAAGCGGCTCGAAAAGCTCATGATGGATCACGCCAAGAACCTCGAATTCGAGAA
GGCGGCGCAGGTGCGCGACCAGCTCGCCAAGCTCAAGGCCCAACTGTTCGGTGCCAGCGGCGAGGAAGCGCCAATGCCGC
CGGTGTAA

Upstream 100 bases:

>100_bases
GGGCGTTGACTGACTTGAAGCTAGGCTGTGCAACCCCAAGATTCTAGCGTATCTGCCCCCGCACTCGCCCCCTTTTCTCA
GGTTTTTCCCCTAGTCGCCT

Downstream 100 bases:

>100_bases
CGCTGCGGCGGCGCGCCGCGCACGGGCTGGCCTATAGTGGAAAGCGTCACGCCAGATCAGGAGATGCGCCATGGTCAAGT
TAGCGCTGTGGGTGCCGCTT

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSNLAEVAPALDEDKIVTFPGSPFQLYQPFPPAGDQPEAIRQLVEGIEDGLSFQTLLGVTGSGKTYTMANVIARMGRPAI
VFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVAT
VSAIYGIGNPNEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTIDIFPAEHAEMAVRLEMFDDEV
ESLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKAELRDRLEFFHKENRLVEVQRLEQRTRFDLEMLSELG
FCKGIENYSRHLSGAKPGEPPPTLVDYLPSDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFEE
FERKMRQVMFVSATPAQFEKEHAGQVVEQVVRPTGLVDPIIIVRPATTQVDDLLSEINLRVEAGERVLVTTLTKRMAEQL
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRA
ARNVNGTAILYADRITDSMRKAIDETERRRAKQMAFNEANGITPRGVIKRIKDIIDGVYDAGEVKAELLAAQERARYEDM
SEKQVSKEIKRLEKLMMDHAKNLEFEKAAQVRDQLAKLKAQLFGASGEEAPMPPV

Sequences:

>Translated_695_residues
MSNLAEVAPALDEDKIVTFPGSPFQLYQPFPPAGDQPEAIRQLVEGIEDGLSFQTLLGVTGSGKTYTMANVIARMGRPAI
VFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVAT
VSAIYGIGNPNEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTIDIFPAEHAEMAVRLEMFDDEV
ESLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKAELRDRLEFFHKENRLVEVQRLEQRTRFDLEMLSELG
FCKGIENYSRHLSGAKPGEPPPTLVDYLPSDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFEE
FERKMRQVMFVSATPAQFEKEHAGQVVEQVVRPTGLVDPIIIVRPATTQVDDLLSEINLRVEAGERVLVTTLTKRMAEQL
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRA
ARNVNGTAILYADRITDSMRKAIDETERRRAKQMAFNEANGITPRGVIKRIKDIIDGVYDAGEVKAELLAAQERARYEDM
SEKQVSKEIKRLEKLMMDHAKNLEFEKAAQVRDQLAKLKAQLFGASGEEAPMPPV
>Mature_694_residues
SNLAEVAPALDEDKIVTFPGSPFQLYQPFPPAGDQPEAIRQLVEGIEDGLSFQTLLGVTGSGKTYTMANVIARMGRPAIV
FAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATV
SAIYGIGNPNEYHQMILTLRTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTIDIFPAEHAEMAVRLEMFDDEVE
SLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKAELRDRLEFFHKENRLVEVQRLEQRTRFDLEMLSELGF
CKGIENYSRHLSGAKPGEPPPTLVDYLPSDALMFLDESHVLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFEEF
ERKMRQVMFVSATPAQFEKEHAGQVVEQVVRPTGLVDPIIIVRPATTQVDDLLSEINLRVEAGERVLVTTLTKRMAEQLT
EFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQTIGRAA
RNVNGTAILYADRITDSMRKAIDETERRRAKQMAFNEANGITPRGVIKRIKDIIDGVYDAGEVKAELLAAQERARYEDMS
EKQVSKEIKRLEKLMMDHAKNLEFEKAAQVRDQLAKLKAQLFGASGEEAPMPPV

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=671, Percent_Identity=67.2131147540984, Blast_Score=891, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=309, Percent_Identity=23.3009708737864, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_CUPPJ (Q473K3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295274.1
- ProteinModelPortal:   Q473K3
- GeneID:   3610503
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A1051
- NMPDR:   fig|264198.3.peg.1554
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- ProtClustDB:   PRK05298
- BioCyc:   REUT264198:REUT_A1051-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 79114; Mature: 78982

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLAEVAPALDEDKIVTFPGSPFQLYQPFPPAGDQPEAIRQLVEGIEDGLSFQTLLGVT
CCCHHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCC
GSGKTYTMANVIARMGRPAIVFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAY
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
VPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATVSAIYGIGNPNEYHQMILTL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHEEEEE
RTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTIDIFPAEHAEMAVRLEMFDDEV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCHHHEEHHHHHHHHH
ESLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKAELRDRLEFFHKENRLV
HHHHHHCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EVQRLEQRTRFDLEMLSELGFCKGIENYSRHLSGAKPGEPPPTLVDYLPSDALMFLDESH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCC
VLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFEEFERKMRQVMFVSATPAQFEK
EEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHEEEECCCHHHHH
EHAGQVVEQVVRPTGLVDPIIIVRPATTQVDDLLSEINLRVEAGERVLVTTLTKRMAEQL
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHH
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILD
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
ADKEGFLRAERSLIQTIGRAARNVNGTAILYADRITDSMRKAIDETERRRAKQMAFNEAN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GITPRGVIKRIKDIIDGVYDAGEVKAELLAAQERARYEDMSEKQVSKEIKRLEKLMMDHA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLEFEKAAQVRDQLAKLKAQLFGASGEEAPMPPV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SNLAEVAPALDEDKIVTFPGSPFQLYQPFPPAGDQPEAIRQLVEGIEDGLSFQTLLGVT
CCHHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCC
GSGKTYTMANVIARMGRPAIVFAPNKTLAAQLYSEFREFFPRNAVEYFVSYYDYYQPEAY
CCCCCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
VPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDTIIVATVSAIYGIGNPNEYHQMILTL
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHCCCCCCHHHHEEEEE
RTGDKISQRDVIARLIAMQYTRNETDFQRGTFRVRGDTIDIFPAEHAEMAVRLEMFDDEV
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCHHHEEHHHHHHHHH
ESLQFFDPLTGRVRQKIPRFTVYPSSHYVTPRETVLRAIEDIKAELRDRLEFFHKENRLV
HHHHHHCHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EVQRLEQRTRFDLEMLSELGFCKGIENYSRHLSGAKPGEPPPTLVDYLPSDALMFLDESH
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCCC
VLIGQLNGMYNGDRARKTTLVEYGFRLPSALDNRPLKFEEFERKMRQVMFVSATPAQFEK
EEEEECCCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHEEEECCCHHHHH
EHAGQVVEQVVRPTGLVDPIIIVRPATTQVDDLLSEINLRVEAGERVLVTTLTKRMAEQL
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCEEEECCCEEHHHHHHHHHHHHH
TEFLSENGVKVRYLHSDIDTVERVEIIRDLRLGTFDVLVGINLLREGLDIPEVSLVAILD
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEE
ADKEGFLRAERSLIQTIGRAARNVNGTAILYADRITDSMRKAIDETERRRAKQMAFNEAN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GITPRGVIKRIKDIIDGVYDAGEVKAELLAAQERARYEDMSEKQVSKEIKRLEKLMMDHA
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KNLEFEKAAQVRDQLAKLKAQLFGASGEEAPMPPV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA