BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is paaF [H]

Identifier: 73540712

GI number: 73540712

Start: 1097624

End: 1098403

Strand: Reverse

Name: paaF [H]

Synonym: Reut_A1009

Alternate gene names: 73540712

Gene position: 1098403-1097624 (Counterclockwise)

Preceding gene: 73540713

Following gene: 73540711

Centisome position: 28.86

GC content: 67.18

Gene sequence:

>780_bases
ATGACCGCACAGTTGCTTACGTCCCGCGTTGATTCGACGCTGGTGCTGACCATCTCGAACCCGGAGGCACGCAATGCGCT
GCATCCGGATATCTACGCCGCCTCTTTCGAGGCGCTGAACGCCGCCGCCGGCGATGATTCGGTGCGCGCCGTGATCATCA
CCGGTGCCGATGGCATCTTCTGCGCCGGCGGCAACCTGAACCGGCTGCTCGGCAACCGCTCGAAGCCGCGCGAAGTGCAG
GCCCAAAGCATCGAAACGCTGAACCACTGGATCGAAACGCTGCACGCGTTCCCCAAGCCTGTCATCGCGGCAGTCGAAGG
CGCGGCGGCGGGGGCCGGTTTCTCGGTCGTGCTGGCGTGCGATTTTGTCGTGGCCGCGAGCGATGCCAAGTTCGTCATGG
CCTACGTCAAGGTCGGACTGACGCCCGACGGCGGCGGTTCGTACGAACTGGCGCGCGCGCTGCCGCGCGCGCTGGCCAGC
GAACTGATGATGGAAGGCAAGCCCGTGGATGGCGCCAGGCTCGCGCAGTTCGGGCTGGTCAACCGTGTGGTGCCACCGGG
CCAAGCGCTGACCGAAGCGTTGCGCCTGGCCGAGGCCATCGCCGAACAGTCCCCGCATGCGGTCGGCCGCATCAAGGGCC
TGATCAACCATGCGGCCACGGCTTCGCTGAGCGAGCACCTCGTGGCCGAGCGCGACAACTTCGTGGCCGCGCTGCACCAC
CCGGACGGCGGCGAAGGCATCAGCGCCTTCCTGGAAAAGCGCAAGCCGCGCTACCGCTGA

Upstream 100 bases:

>100_bases
CCCGTGGCTGTGGCGCCGTGCCGGTCTCGGCCTGTCGCTGCTGACCGAAGAGAACTGAGGAGCGTGGCTCCCCTGCCCCC
GCAATATCCCGAGGCCGTCC

Downstream 100 bases:

>100_bases
ACATTGCGTTGAACACGGCGGCGCGGTAGCGTGCCGCCGCAGAAGCCCGCGCAAGACGGGCCTGCTGTCATCAATGAGGG
AGACAAGCATGGAATTGCCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTAQLLTSRVDSTLVLTISNPEARNALHPDIYAASFEALNAAAGDDSVRAVIITGADGIFCAGGNLNRLLGNRSKPREVQ
AQSIETLNHWIETLHAFPKPVIAAVEGAAAGAGFSVVLACDFVVAASDAKFVMAYVKVGLTPDGGGSYELARALPRALAS
ELMMEGKPVDGARLAQFGLVNRVVPPGQALTEALRLAEAIAEQSPHAVGRIKGLINHAATASLSEHLVAERDNFVAALHH
PDGGEGISAFLEKRKPRYR

Sequences:

>Translated_259_residues
MTAQLLTSRVDSTLVLTISNPEARNALHPDIYAASFEALNAAAGDDSVRAVIITGADGIFCAGGNLNRLLGNRSKPREVQ
AQSIETLNHWIETLHAFPKPVIAAVEGAAAGAGFSVVLACDFVVAASDAKFVMAYVKVGLTPDGGGSYELARALPRALAS
ELMMEGKPVDGARLAQFGLVNRVVPPGQALTEALRLAEAIAEQSPHAVGRIKGLINHAATASLSEHLVAERDNFVAALHH
PDGGEGISAFLEKRKPRYR
>Mature_258_residues
TAQLLTSRVDSTLVLTISNPEARNALHPDIYAASFEALNAAAGDDSVRAVIITGADGIFCAGGNLNRLLGNRSKPREVQA
QSIETLNHWIETLHAFPKPVIAAVEGAAAGAGFSVVLACDFVVAASDAKFVMAYVKVGLTPDGGGSYELARALPRALASE
LMMEGKPVDGARLAQFGLVNRVVPPGQALTEALRLAEAIAEQSPHAVGRIKGLINHAATASLSEHLVAERDNFVAALHHP
DGGEGISAFLEKRKPRYR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=31.4285714285714, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=30.4, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI25453481, Length=205, Percent_Identity=27.8048780487805, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI51538573, Length=205, Percent_Identity=27.8048780487805, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4757966, Length=205, Percent_Identity=27.8048780487805, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI51538581, Length=205, Percent_Identity=27.8048780487805, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI116235487, Length=204, Percent_Identity=30.3921568627451, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI4757968, Length=205, Percent_Identity=27.3170731707317, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI49355787, Length=205, Percent_Identity=27.3170731707317, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221136756, Length=246, Percent_Identity=25.609756097561, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI221136753, Length=246, Percent_Identity=25.609756097561, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI221307494, Length=207, Percent_Identity=28.5024154589372, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI70995211, Length=244, Percent_Identity=30.327868852459, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=29.5454545454545, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=212, Percent_Identity=26.8867924528302, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI45643119, Length=212, Percent_Identity=26.8867924528302, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI260275230, Length=212, Percent_Identity=26.8867924528302, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI68989263, Length=197, Percent_Identity=28.4263959390863, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=35.7976653696498, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787660, Length=246, Percent_Identity=38.2113821138211, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=30.6818181818182, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=28.4, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=27.9816513761468, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=30.8457711442786, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790281, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=33.6065573770492, Blast_Score=134, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.3492063492063, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=230, Percent_Identity=29.1304347826087, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=31.6872427983539, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=24.901185770751, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=26.3565891472868, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=201, Percent_Identity=27.363184079602, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=212, Percent_Identity=27.8301886792453, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=246, Percent_Identity=25.2032520325203, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=238, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=244, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=244, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=244, Percent_Identity=25, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=32.9268292682927, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=32.9268292682927, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653139, Length=265, Percent_Identity=32.4528301886792, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922422, Length=244, Percent_Identity=28.6885245901639, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654903, Length=254, Percent_Identity=25.1968503937008, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=29.6116504854369, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=200, Percent_Identity=28, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=23.3463035019455, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27233; Mature: 27102

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLLTSRVDSTLVLTISNPEARNALHPDIYAASFEALNAAAGDDSVRAVIITGADGIF
CCHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
CAGGNLNRLLGNRSKPREVQAQSIETLNHWIETLHAFPKPVIAAVEGAAAGAGFSVVLAC
ECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEH
DFVVAASDAKFVMAYVKVGLTPDGGGSYELARALPRALASELMMEGKPVDGARLAQFGLV
HHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NRVVPPGQALTEALRLAEAIAEQSPHAVGRIKGLINHAATASLSEHLVAERDNFVAALHH
HCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PDGGEGISAFLEKRKPRYR
CCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TAQLLTSRVDSTLVLTISNPEARNALHPDIYAASFEALNAAAGDDSVRAVIITGADGIF
CHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEE
CAGGNLNRLLGNRSKPREVQAQSIETLNHWIETLHAFPKPVIAAVEGAAAGAGFSVVLAC
ECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEH
DFVVAASDAKFVMAYVKVGLTPDGGGSYELARALPRALASELMMEGKPVDGARLAQFGLV
HHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NRVVPPGQALTEALRLAEAIAEQSPHAVGRIKGLINHAATASLSEHLVAERDNFVAALHH
HCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PDGGEGISAFLEKRKPRYR
CCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]