Click here to switch to the map view.

The map label for this gene is leuA1 [H]

Identifier: 73540656

GI number: 73540656

Start: 1039800

End: 1041341

Strand: Direct

Name: leuA1 [H]

Synonym: Reut_A0953

Alternate gene names: 73540656

Gene position: 1039800-1041341 (Clockwise)

Preceding gene: 73540655

Following gene: 73540658

Centisome position: 27.32

GC content: 63.29

Gene sequence:

>1542_bases
ATGTCTGACAAACTCATCATTTTCGATACCACCTTGCGCGATGGCGAGCAGTCGCCCGGCGCCTCGATGACGCGCGAGGA
GAAGATCCGCATTGCGAAGCAACTGGAGCGCCTGAAGGTCGATGTGATCGAAGCGGGCTTTGCGGCAAGCTCCAACGGCG
ACTACGAAGCCATTCGCTCGATCGCCCAGGTCGTGAAGGATTCCACGATCTGCTCGCTGGCCCGTGCCAACGACAAGGAC
ATCGCGCGTGCCGCCGAGGCCCTCAAGCCGGCCAACTCGTTCCGTATCCACACGTTCATCGCCACGTCGGCGCTGCACAT
GGAGAAGAAGCTGCGCATGACGCCGGACCAGGTCTATGAGCAGGCCCGCCTGGCCGTGCGCTTCGCGCGCCAGTTCACGG
ACGATATCGAGTTCTCGCCGGAAGACGGCAGCCGCTCCGACATGGACTTCCTGTGCCGCGTGCTCGAAGGCGTGATCGCC
GAGGGCGCGACCACCATCAACCTTCCGGACACCGTGGGCTATGCGGTGCCGGAAGGCTACGCCGACCTGATCCGCTCGGT
GCGCGAGCGCATTCCGAACTCGGACAAGGCTGTGTGGTCCGTGCACTGCCACAACGACCTCGGCATGGCCGTGGCGAACT
CGCTGGCCGCGGTGAAGCTCGGCGGCGCGCGCCAGATCGAATGCACGATCAACGGTCTCGGCGAGCGCGCCGGCAACACC
AGCCTTGAAGAAGTCGTGATGGCCGTGAAGACCCGCCGCGACTACTTCAATCTCGATGTCGGTGTCGACACCTCGCAGAT
CGTGCCAGCCTCGAAGCTGGTGTCGCAGATCACCGGTTTCGTGGTGCAGCCGAACAAGGCCGTGGTTGGTGCCAACGCGT
TCGCGCATGCATCGGGCATCCACCAGGATGGCGTCCTGAAGGCGCGCGACACCTACGAGATCATGCGCGCGGAGGATGTA
GGCTGGACCGCCAACAAGATCGTGCTGGGCAAGCTGTCGGGCCGCAATGCATTCAAGCAGCGCCTGCAGGAACTGGGCGT
CGAGCTTGAAAGCGAGGCTGAAGTCAACGCCGCGTTCGGCCGCTTCAAGGAACTCGCCGACCAGAAGGCCGAGATCTTCG
ATGAGGACATCATGGCCATCGTTTCGAACGAGGCCCAGCACGACGCCAATGAGCACTATCGCTTCATTTCGCTGTCGCAG
CGCTCGGAAACCGGCGAGCGTCCGCATGCCCGCGTCGTGTTCAACATGGACGGCCAGGAGCATTCCGGTGAAGGCGAGGG
CAACGGTCCGGTCGACGCTACGCTGCATGCGATCGAATCGCAGGTGAACAGCGGCGCGGAGATGGTGCTGTATTCGGTGA
ACGCCATCACGGGCGGCACCGAGGCGCAGGGCGAAGTGACGGTGCGCCTGTCCAAGGCCGGCCGCATCGTCAATGGCGTC
GGTACCGATCCGGACATCGTTGCAGCTTCGGCAAAGGCCTACCTGGCCGCCCTGAACAAGCTGCACGACAAGGCCGTGCA
AAAGATCAATCCGCAGATCTGA

Upstream 100 bases:

>100_bases
CCGGGGCGTTTTGCATTCACTCGCAGCGAATGCGTGGAACAGGCGGTACCCGGCGGCACGATGGCGGGGACAGCACACGA
TAAAGCCCAGGAGCTCCACA

Downstream 100 bases:

>100_bases
TTTTCAGACCCGCATCGTGGCGCCACTCGTAATCCGGGTGGCGCCTGCGGCGAAGAGCCTATCCGGCCTCAACGCCCGAA
CCAGCCCGCCGGCCGCCAGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIAKQLERLKVDVIEAGFAASSNGDYEAIRSIAQVVKDSTICSLARANDKD
IARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYADLIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTSQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVELESEAEVNAAFGRFKELADQKAEIFDEDIMAIVSNEAQHDANEHYRFISLSQ
RSETGERPHARVVFNMDGQEHSGEGEGNGPVDATLHAIESQVNSGAEMVLYSVNAITGGTEAQGEVTVRLSKAGRIVNGV
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI

Sequences:

>Translated_513_residues
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIAKQLERLKVDVIEAGFAASSNGDYEAIRSIAQVVKDSTICSLARANDKD
IARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIA
EGATTINLPDTVGYAVPEGYADLIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
SLEEVVMAVKTRRDYFNLDVGVDTSQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVELESEAEVNAAFGRFKELADQKAEIFDEDIMAIVSNEAQHDANEHYRFISLSQ
RSETGERPHARVVFNMDGQEHSGEGEGNGPVDATLHAIESQVNSGAEMVLYSVNAITGGTEAQGEVTVRLSKAGRIVNGV
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
>Mature_512_residues
SDKLIIFDTTLRDGEQSPGASMTREEKIRIAKQLERLKVDVIEAGFAASSNGDYEAIRSIAQVVKDSTICSLARANDKDI
ARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYEQARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAE
GATTINLPDTVGYAVPEGYADLIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNTS
LEEVVMAVKTRRDYFNLDVGVDTSQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGVELESEAEVNAAFGRFKELADQKAEIFDEDIMAIVSNEAQHDANEHYRFISLSQR
SETGERPHARVVFNMDGQEHSGEGEGNGPVDATLHAIESQVNSGAEMVLYSVNAITGGTEAQGEVTVRLSKAGRIVNGVG
TDPDIVAASAKAYLAALNKLHDKAVQKINPQI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=48.502994011976, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=26.7657992565056, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=540, Percent_Identity=27.2222222222222, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=28.0104712041885, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=28.0104712041885, Blast_Score=127, Evalue=4e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55696; Mature: 55565

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLIIFDTTLRDGEQSPGASMTREEKIRIAKQLERLKVDVIEAGFAASSNGDYEAIRS
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQVVKDSTICSLARANDKDIARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCH
ADLIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTSQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVELESEAEVNAAFG
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIMAIVSNEAQHDANEHYRFISLSQRSETGERPHARVVFNMDGQE
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCC
HSGEGEGNGPVDATLHAIESQVNSGAEMVLYSVNAITGGTEAQGEVTVRLSKAGRIVNGV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDKLIIFDTTLRDGEQSPGASMTREEKIRIAKQLERLKVDVIEAGFAASSNGDYEAIRS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAQVVKDSTICSLARANDKDIARAAEALKPANSFRIHTFIATSALHMEKKLRMTPDQVYE
HHHHHHCHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHH
QARLAVRFARQFTDDIEFSPEDGSRSDMDFLCRVLEGVIAEGATTINLPDTVGYAVPEGY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCH
ADLIRSVRERIPNSDKAVWSVHCHNDLGMAVANSLAAVKLGGARQIECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCHHEEEECCCEEEEEEEECCCCCCCCC
SLEEVVMAVKTRRDYFNLDVGVDTSQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCEEEECCCCHHHCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVELESEAEVNAAFG
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHH
RFKELADQKAEIFDEDIMAIVSNEAQHDANEHYRFISLSQRSETGERPHARVVFNMDGQE
HHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCC
HSGEGEGNGPVDATLHAIESQVNSGAEMVLYSVNAITGGTEAQGEVTVRLSKAGRIVNGV
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCEEECC
GTDPDIVAASAKAYLAALNKLHDKAVQKINPQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]