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The map label for this gene is ecpD [H]

Identifier: 73540542

GI number: 73540542

Start: 908074

End: 908829

Strand: Reverse

Name: ecpD [H]

Synonym: Reut_A0839

Alternate gene names: 73540542

Gene position: 908829-908074 (Counterclockwise)

Preceding gene: 73540543

Following gene: 73540541

Centisome position: 23.88

GC content: 63.23

Gene sequence:

>756_bases
ATGAACCGATCGATCAGGAACGTCATTGGCGCCTGCGCAATGACGCTGGTACTGCTGTCCCACTCGCACGCGTCCGTCGT
CATCGCCGGCACACGTGTCATCGTCCATGCAGGCGATGGGGAAGCCACCATCAAGCTCAGCAATGAAGGCAAATCCCCCG
CCCTGACCCAGGCATGGCTCGACACAGGCGATGCTTCGGCACAGCCCGCCGGTATCGATGTGCCATTCACCGTCACACCA
CCGATCGCGCGCATCGAACCAGGGAAGGCGCAGACACTGCGCATTCACTACACCGGCGAGCCGTTGCCGGCCGACAAGGA
ATCGGTGTTCTGGCTCAGTGTGCTCGAAGTCCCTCCGAAGCCCGGAGCCGACGAGATCGATGCGAACAAGCTCCAGCTGG
CGTTTCGGTCGCGCATCAAGCTCTTCTTCCGTCCTGCCGGCCTGCATGGCGAAGCCACCGAAGCGCCGGCACGCGTTGCA
TGGCGGCTTGTCAAAGCCGACCGTGGCCTCGCGCTGGAGGCCAGCAACCCGACTGCCTACCACGTCTCGTTCTCCAGCGT
GGAATTGCTGGATGGTACGCACAGCGCCAGCAACGAAGCTGGCGGCATGGTGGCGCCGGGCAAATCGATGCGATTTCCGC
TTGCCGGCAATGTGGATCCAGGCAAAGCCATCACCGTTCGCTACCAGGCCATCAACGACTATGGCGGCCCTATCGACGGC
GAAGCTCACCCGAACCGGTCCGCCGCTGCCCGATAG

Upstream 100 bases:

>100_bases
CGTGATGTACACCATCGTCTATCAGTAAGCATGCAACGCCCGGGTGCGCGGCCTTTCAAGCCGCCGCACCCGCCGCCTGC
ATTCGCGTGTTGCCCCCATC

Downstream 100 bases:

>100_bases
CGCCTGCTCCAACCGCCGGCACATGCCGGCCTCACTGAAATTCCCCTGATTCGCGCTGACGCATGCCGCTGGACGGCAGG
GACGCGTGTTACCCCAATTT

Product: pili assembly chaperone: pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNRSIRNVIGACAMTLVLLSHSHASVVIAGTRVIVHAGDGEATIKLSNEGKSPALTQAWLDTGDASAQPAGIDVPFTVTP
PIARIEPGKAQTLRIHYTGEPLPADKESVFWLSVLEVPPKPGADEIDANKLQLAFRSRIKLFFRPAGLHGEATEAPARVA
WRLVKADRGLALEASNPTAYHVSFSSVELLDGTHSASNEAGGMVAPGKSMRFPLAGNVDPGKAITVRYQAINDYGGPIDG
EAHPNRSAAAR

Sequences:

>Translated_251_residues
MNRSIRNVIGACAMTLVLLSHSHASVVIAGTRVIVHAGDGEATIKLSNEGKSPALTQAWLDTGDASAQPAGIDVPFTVTP
PIARIEPGKAQTLRIHYTGEPLPADKESVFWLSVLEVPPKPGADEIDANKLQLAFRSRIKLFFRPAGLHGEATEAPARVA
WRLVKADRGLALEASNPTAYHVSFSSVELLDGTHSASNEAGGMVAPGKSMRFPLAGNVDPGKAITVRYQAINDYGGPIDG
EAHPNRSAAAR
>Mature_251_residues
MNRSIRNVIGACAMTLVLLSHSHASVVIAGTRVIVHAGDGEATIKLSNEGKSPALTQAWLDTGDASAQPAGIDVPFTVTP
PIARIEPGKAQTLRIHYTGEPLPADKESVFWLSVLEVPPKPGADEIDANKLQLAFRSRIKLFFRPAGLHGEATEAPARVA
WRLVKADRGLALEASNPTAYHVSFSSVELLDGTHSASNEAGGMVAPGKSMRFPLAGNVDPGKAITVRYQAINDYGGPIDG
EAHPNRSAAAR

Specific function: Probably mediates assembly of a pilin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=254, Percent_Identity=41.7322834645669, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789532, Length=221, Percent_Identity=37.10407239819, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1788428, Length=240, Percent_Identity=34.5833333333333, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1787171, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786743, Length=218, Percent_Identity=31.1926605504587, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790771, Length=227, Percent_Identity=34.3612334801762, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788677, Length=211, Percent_Identity=30.3317535545024, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1786936, Length=205, Percent_Identity=31.219512195122, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081806, Length=227, Percent_Identity=29.9559471365639, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87082203, Length=243, Percent_Identity=27.1604938271605, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26509; Mature: 26509

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSIRNVIGACAMTLVLLSHSHASVVIAGTRVIVHAGDGEATIKLSNEGKSPALTQAWL
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHH
DTGDASAQPAGIDVPFTVTPPIARIEPGKAQTLRIHYTGEPLPADKESVFWLSVLEVPPK
CCCCCCCCCCCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCC
PGADEIDANKLQLAFRSRIKLFFRPAGLHGEATEAPARVAWRLVKADRGLALEASNPTAY
CCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHEEEEECCCCEEEECCCCEEE
HVSFSSVELLDGTHSASNEAGGMVAPGKSMRFPLAGNVDPGKAITVRYQAINDYGGPIDG
EEEECEEEEECCCCCCCCCCCCEECCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCCC
EAHPNRSAAAR
CCCCCCCCCCC
>Mature Secondary Structure
MNRSIRNVIGACAMTLVLLSHSHASVVIAGTRVIVHAGDGEATIKLSNEGKSPALTQAWL
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHH
DTGDASAQPAGIDVPFTVTPPIARIEPGKAQTLRIHYTGEPLPADKESVFWLSVLEVPPK
CCCCCCCCCCCCCCCEEECCCCCEECCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCC
PGADEIDANKLQLAFRSRIKLFFRPAGLHGEATEAPARVAWRLVKADRGLALEASNPTAY
CCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCCHHHHHEEEEECCCCEEEECCCCEEE
HVSFSSVELLDGTHSASNEAGGMVAPGKSMRFPLAGNVDPGKAITVRYQAINDYGGPIDG
EEEECEEEEECCCCCCCCCCCCEECCCCCEECCCCCCCCCCCEEEEEEEEECCCCCCCCC
EAHPNRSAAAR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]