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The map label for this gene is tolB

Identifier: 73540499

GI number: 73540499

Start: 864671

End: 866023

Strand: Direct

Name: tolB

Synonym: Reut_A0795

Alternate gene names: 73540499

Gene position: 864671-866023 (Clockwise)

Preceding gene: 73540498

Following gene: 73540500

Centisome position: 22.72

GC content: 65.78

Gene sequence:

>1353_bases
ATGCGAAAGCTTTGGGCCCCCAACTGGCTGTCCGCGAAGCGGCACCACGCAAACCAGGCCGCGACCCGATTGATTGGCCG
GCACGCACTGATGGCCTGGCTGGCCGCCGCGCTGGCCTTGTCCGCCGGCGCCGCACAGGCGCAACTGAACGTGGAGATCT
CCGGTGTTGGCGCGAGCCAGATCCCGGTCGCCACGGCGAACTTCCAGGGCGAGGCCCAAGCCCCGCAGAACCTAACCGCC
ATCATTCGCAGCGACCTCGCGCGCAGCGGCCGTCTCCGCAACGTTGATCCGGGCGGTGCCACGGTGGCCGAGTCGGCCAA
TGTCGATCTCGGAAGCTGGAAAAGCCGCGGCGCCGATGCCTTCGTTGCAGGCAGCGTGACGCCTTCGGGCAATGGCCAGT
ACGAGGTGCGCTTCCGCCTTTACGACACCGTCAAGGGCCAGAGCCTGGGCGGGCTGGCGTTCAACGTCAACCAGGGCCAG
TTGCGTGTCACGGCTCACAAGATCGCCGACTACATCTACGAGAAGCTGCTCGGCGAGCGTGGCGTGTTCGCGACGCGCCT
GTCCTATGTGTCGCGTGTCGGCGGCCGCTACCAGTTGCTGATCTCCGATTCGGATGGCCAGAACTCGCAGGTTGCGCTGA
CCAGCAACGAGCCGATCATCTCGCCGTCGTGGTCGCCGGACGGCCGCCGCGTGGCCTATGTCTCGTTCGAAGCCAAGAAG
CCGGTGGTGTACGTGCACGACCTGGCCACGGGCAAGCGCACGCTGGTGTCGAACCAGAAGGGCAATAACAGCGCGCCGTC
GTGGTCGCCCGATGGGCAGCACCTGGCCGTGGCGCTGTCGCGCGATGGCAATACCCAGGTGTACCAAGTCAATGCCGATG
GTTCGGGTATTCGCCGCCTGACCCGCAGCAGCGCGATCGACACCGAGCCGCAGTATTCGCCTGACGGCAAGAGCATCTAT
TTCACCAGCGACCGCGGCGGTGCACCGCAGGTCTATCGCATGCCGGCAGGCGGCGAGGAAGCGGGCAGCGCGCAGCGCGT
GACCTTCAAGGGCAGCTACAACGTGAGCCCACGGATTTCGCCGGACGGCAAGTATCTGGCGTACATCACGCGCTCGGGCG
GCTTCAAGCTGCAGCTGCAGGACCTGACCAACGGCGACGTCACTTCGCTGACCGATACGTCCAATGACGAAGCGCCGAGC
TTTGCCGCGAACGGCAAGTACATCCTATACGCCACCCGCGTGGGCGGCCGCTCGGTACTGGCGGCGGTGTCCACTGACGG
GCGTACCCGGCAGGTTCTGTCTCTCCAGTCCGGCGATGTCCGCGAGCCGTCCTGGGGTCCTTTCATGCAATAA

Upstream 100 bases:

>100_bases
GCGATCTGCACGCGGAACGGGCATGCGCCGTGCGCATCCACGCCTGTTCCGCTGTCTGCCTGCCGTGCCCGATGAATCTT
GAAATCGACTGAGGAGCAGC

Downstream 100 bases:

>100_bases
CGCAATAACAGGAGTCTCTCGCCATGCCCCAACTGATGACTAAAACCCTTGCCGTCGCCGCCCTGCTGGCCCTAGGTGCC
TGCAGCTCCGGCGTGAAGCT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRKLWAPNWLSAKRHHANQAATRLIGRHALMAWLAAALALSAGAAQAQLNVEISGVGASQIPVATANFQGEAQAPQNLTA
IIRSDLARSGRLRNVDPGGATVAESANVDLGSWKSRGADAFVAGSVTPSGNGQYEVRFRLYDTVKGQSLGGLAFNVNQGQ
LRVTAHKIADYIYEKLLGERGVFATRLSYVSRVGGRYQLLISDSDGQNSQVALTSNEPIISPSWSPDGRRVAYVSFEAKK
PVVYVHDLATGKRTLVSNQKGNNSAPSWSPDGQHLAVALSRDGNTQVYQVNADGSGIRRLTRSSAIDTEPQYSPDGKSIY
FTSDRGGAPQVYRMPAGGEEAGSAQRVTFKGSYNVSPRISPDGKYLAYITRSGGFKLQLQDLTNGDVTSLTDTSNDEAPS
FAANGKYILYATRVGGRSVLAAVSTDGRTRQVLSLQSGDVREPSWGPFMQ

Sequences:

>Translated_450_residues
MRKLWAPNWLSAKRHHANQAATRLIGRHALMAWLAAALALSAGAAQAQLNVEISGVGASQIPVATANFQGEAQAPQNLTA
IIRSDLARSGRLRNVDPGGATVAESANVDLGSWKSRGADAFVAGSVTPSGNGQYEVRFRLYDTVKGQSLGGLAFNVNQGQ
LRVTAHKIADYIYEKLLGERGVFATRLSYVSRVGGRYQLLISDSDGQNSQVALTSNEPIISPSWSPDGRRVAYVSFEAKK
PVVYVHDLATGKRTLVSNQKGNNSAPSWSPDGQHLAVALSRDGNTQVYQVNADGSGIRRLTRSSAIDTEPQYSPDGKSIY
FTSDRGGAPQVYRMPAGGEEAGSAQRVTFKGSYNVSPRISPDGKYLAYITRSGGFKLQLQDLTNGDVTSLTDTSNDEAPS
FAANGKYILYATRVGGRSVLAAVSTDGRTRQVLSLQSGDVREPSWGPFMQ
>Mature_450_residues
MRKLWAPNWLSAKRHHANQAATRLIGRHALMAWLAAALALSAGAAQAQLNVEISGVGASQIPVATANFQGEAQAPQNLTA
IIRSDLARSGRLRNVDPGGATVAESANVDLGSWKSRGADAFVAGSVTPSGNGQYEVRFRLYDTVKGQSLGGLAFNVNQGQ
LRVTAHKIADYIYEKLLGERGVFATRLSYVSRVGGRYQLLISDSDGQNSQVALTSNEPIISPSWSPDGRRVAYVSFEAKK
PVVYVHDLATGKRTLVSNQKGNNSAPSWSPDGQHLAVALSRDGNTQVYQVNADGSGIRRLTRSSAIDTEPQYSPDGKSIY
FTSDRGGAPQVYRMPAGGEEAGSAQRVTFKGSYNVSPRISPDGKYLAYITRSGGFKLQLQDLTNGDVTSLTDTSNDEAPS
FAANGKYILYATRVGGRSVLAAVSTDGRTRQVLSLQSGDVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=428, Percent_Identity=37.3831775700935, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_CUPPJ (Q474K8)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295019.1
- ProteinModelPortal:   Q474K8
- SMR:   Q474K8
- GeneID:   3610514
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0795
- NMPDR:   fig|264198.3.peg.1309
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK02889
- BioCyc:   REUT264198:REUT_A0795-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48142; Mature: 48142

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLWAPNWLSAKRHHANQAATRLIGRHALMAWLAAALALSAGAAQAQLNVEISGVGASQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
IPVATANFQGEAQAPQNLTAIIRSDLARSGRLRNVDPGGATVAESANVDLGSWKSRGADA
CCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCE
FVAGSVTPSGNGQYEVRFRLYDTVKGQSLGGLAFNVNQGQLRVTAHKIADYIYEKLLGER
EEEECCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCC
GVFATRLSYVSRVGGRYQLLISDSDGQNSQVALTSNEPIISPSWSPDGRRVAYVSFEAKK
CHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEEECCC
PVVYVHDLATGKRTLVSNQKGNNSAPSWSPDGQHLAVALSRDGNTQVYQVNADGSGIRRL
CEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHH
TRSSAIDTEPQYSPDGKSIYFTSDRGGAPQVYRMPAGGEEAGSAQRVTFKGSYNVSPRIS
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEC
PDGKYLAYITRSGGFKLQLQDLTNGDVTSLTDTSNDEAPSFAANGKYILYATRVGGRSVL
CCCCEEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCEECCCCEEEEEEECCCCEEE
AAVSTDGRTRQVLSLQSGDVREPSWGPFMQ
EEECCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLWAPNWLSAKRHHANQAATRLIGRHALMAWLAAALALSAGAAQAQLNVEISGVGASQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
IPVATANFQGEAQAPQNLTAIIRSDLARSGRLRNVDPGGATVAESANVDLGSWKSRGADA
CCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCE
FVAGSVTPSGNGQYEVRFRLYDTVKGQSLGGLAFNVNQGQLRVTAHKIADYIYEKLLGER
EEEECCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHCCC
GVFATRLSYVSRVGGRYQLLISDSDGQNSQVALTSNEPIISPSWSPDGRRVAYVSFEAKK
CHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEEECCC
PVVYVHDLATGKRTLVSNQKGNNSAPSWSPDGQHLAVALSRDGNTQVYQVNADGSGIRRL
CEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHH
TRSSAIDTEPQYSPDGKSIYFTSDRGGAPQVYRMPAGGEEAGSAQRVTFKGSYNVSPRIS
HHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCEEC
PDGKYLAYITRSGGFKLQLQDLTNGDVTSLTDTSNDEAPSFAANGKYILYATRVGGRSVL
CCCCEEEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCEECCCCEEEEEEECCCCEEE
AAVSTDGRTRQVLSLQSGDVREPSWGPFMQ
EEECCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA