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The map label for this gene is nuoF2 [H]

Identifier: 73540324

GI number: 73540324

Start: 664295

End: 665860

Strand: Direct

Name: nuoF2 [H]

Synonym: Reut_A0618

Alternate gene names: 73540324

Gene position: 664295-665860 (Clockwise)

Preceding gene: 73540323

Following gene: 73540325

Centisome position: 17.45

GC content: 66.48

Gene sequence:

>1566_bases
ATGAGCGCCGCCGTCACCACCATCTTTGTCCCGCGCGATTCCACCGCGCTGGCCCTTGGCGCCGACGACGTGGCGCGCGC
GATTGCTCGTGAAGCGGCTGCGCGCGGCGAGTCGGTGCACATCGTGCGCAACGGCTCGCGCGGCATGTTCTGGCTCGAGC
CGTTGGTTGAAGTGCAAACCGAAGCGGGCCGCGCGGCCTACGGTCCAGTCAGCGTGGACGATGTGCCGGGACTGCTAGAT
GCGGGCCTGCTGCAAGGCGGCGAACACGTACTGGCTCATGGCCTCACCGACGAGATTCCTTTCCTCAAGCGCCAGGAACG
CCTGACGTTCGCGCGTGTCGGCATCACCGATCCGCTGTCGCTCGACGACTACCGCGCGCATGAAGGCTATGCCGGCCTCG
AACGCGCGCTGGCGATGGAGCCTGCCGCGATCGTGCAGGAAGTGACCGACTCCGGCCTGCGTGGCCGTGGCGGCGCGGCG
TTTCCCACCGGCATTAAGTGGAAGACCGTGCTCGGCGCGAAGTCCGCGGTCAAGTACATCGTCTGCAATGCCGACGAAGG
CGATTCGGGCACGTTCTCCGACCGCATGGTGATGGAAGACGACCCGTTCGTGCTGATCGAGGGCATGACCATCGCCGGTC
TCGCGGTCGGTGCGGAGTACGGCTATATCTACTGCCGTTCCGAATACCCGCATGCGATTGCCGTGCTGAATGCAGCCATT
GCTACGGCTACGGCCGCCGGCTGGCTCGGCGACGACATCCGCGGCAGCGGCAAGCGCTTCCACCTGGAAGTGCGCAAGGG
CGCCGGCGCCTATGTGTGCGGCGAGGAAACCGCGCTGCTCGAAAGCCTGGAAGGTAAACGTGGCGTGGTGCGCGCCAAGC
CGCCGCTCCCGGCGCTGGAGGGCCTGTTTGGCAAGCCCACCGTCATCAACAACGTGATTTCGCTCGCGACGGTGCCCGTC
ATCCTCGCGCGCGGCGCGCAGCACTACCGCGACTTCGGCATGGGTCGTTCACGCGGCACGCTGCCGTTCCAGCTCGCCGG
CAATATCAAGCAAGGTGGTCTGGTCGAAAAGGCATTCGGCGTGACGCTGCGTGAACTGCTCTACGACTACGGCGGTGGCA
CGCGCAGCGGCCGCGCCGTACGCGCGGTGCAGGTGGGCGGCCCGCTCGGCGCCTATCTGCCCGAAGCGCGCTTCGATGTG
CCGATGGACTACGAAGCCTATGCGGCATTCGGTGGTGTAGTCGGCCACGGCGGCATTGTGGTGTTCGACGAGACCGTCGA
CATGGCCAGGCAGGCGCGCTACGCGATGGAGTTCTGCGCGATCGAATCATGTGGCAAGTGCACGCCGTGCCGGATCGGCT
CCACGCGCGGCGTGGAGGTCATGGACCGGATCATCGCCGGCAACGAGCCCGTCAAGCATGTGAAGCTGGTGCGCGACCTG
TGCGACACCATGCTCAACGGCTCGCTCTGCGCCATGGGCGGTATGACGCCGTACCCGGTGCTGTCCGCACTCAACGAATT
CCCCGAGGACTTCGGCCTCGCCTCCAACCCAGCCAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
AACTGCACGGCTATGTCGACGCAAAGCGCTTCGACGTGCTGGTGCGCAAGCTGCGCGCCGACCAGACCGCCCACCAGCCC
GCCAATCAGGAGACCCAGGC

Downstream 100 bases:

>100_bases
GCCTGCGCCAGAACAGAACTACGGGAGACAACCATGGATTGCCGCGATGTGATTGACTTCGGCACCCCCGCCAGCGAATC
GACCGAAATGGTCACCCTCG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSAAVTTIFVPRDSTALALGADDVARAIAREAAARGESVHIVRNGSRGMFWLEPLVEVQTEAGRAAYGPVSVDDVPGLLD
AGLLQGGEHVLAHGLTDEIPFLKRQERLTFARVGITDPLSLDDYRAHEGYAGLERALAMEPAAIVQEVTDSGLRGRGGAA
FPTGIKWKTVLGAKSAVKYIVCNADEGDSGTFSDRMVMEDDPFVLIEGMTIAGLAVGAEYGYIYCRSEYPHAIAVLNAAI
ATATAAGWLGDDIRGSGKRFHLEVRKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALEGLFGKPTVINNVISLATVPV
ILARGAQHYRDFGMGRSRGTLPFQLAGNIKQGGLVEKAFGVTLRELLYDYGGGTRSGRAVRAVQVGGPLGAYLPEARFDV
PMDYEAYAAFGGVVGHGGIVVFDETVDMARQARYAMEFCAIESCGKCTPCRIGSTRGVEVMDRIIAGNEPVKHVKLVRDL
CDTMLNGSLCAMGGMTPYPVLSALNEFPEDFGLASNPAKAA

Sequences:

>Translated_521_residues
MSAAVTTIFVPRDSTALALGADDVARAIAREAAARGESVHIVRNGSRGMFWLEPLVEVQTEAGRAAYGPVSVDDVPGLLD
AGLLQGGEHVLAHGLTDEIPFLKRQERLTFARVGITDPLSLDDYRAHEGYAGLERALAMEPAAIVQEVTDSGLRGRGGAA
FPTGIKWKTVLGAKSAVKYIVCNADEGDSGTFSDRMVMEDDPFVLIEGMTIAGLAVGAEYGYIYCRSEYPHAIAVLNAAI
ATATAAGWLGDDIRGSGKRFHLEVRKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALEGLFGKPTVINNVISLATVPV
ILARGAQHYRDFGMGRSRGTLPFQLAGNIKQGGLVEKAFGVTLRELLYDYGGGTRSGRAVRAVQVGGPLGAYLPEARFDV
PMDYEAYAAFGGVVGHGGIVVFDETVDMARQARYAMEFCAIESCGKCTPCRIGSTRGVEVMDRIIAGNEPVKHVKLVRDL
CDTMLNGSLCAMGGMTPYPVLSALNEFPEDFGLASNPAKAA
>Mature_520_residues
SAAVTTIFVPRDSTALALGADDVARAIAREAAARGESVHIVRNGSRGMFWLEPLVEVQTEAGRAAYGPVSVDDVPGLLDA
GLLQGGEHVLAHGLTDEIPFLKRQERLTFARVGITDPLSLDDYRAHEGYAGLERALAMEPAAIVQEVTDSGLRGRGGAAF
PTGIKWKTVLGAKSAVKYIVCNADEGDSGTFSDRMVMEDDPFVLIEGMTIAGLAVGAEYGYIYCRSEYPHAIAVLNAAIA
TATAAGWLGDDIRGSGKRFHLEVRKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALEGLFGKPTVINNVISLATVPVI
LARGAQHYRDFGMGRSRGTLPFQLAGNIKQGGLVEKAFGVTLRELLYDYGGGTRSGRAVRAVQVGGPLGAYLPEARFDVP
MDYEAYAAFGGVVGHGGIVVFDETVDMARQARYAMEFCAIESCGKCTPCRIGSTRGVEVMDRIIAGNEPVKHVKLVRDLC
DTMLNGSLCAMGGMTPYPVLSALNEFPEDFGLASNPAKAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=373, Percent_Identity=38.8739946380697, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI260656005, Length=373, Percent_Identity=38.8739946380697, Blast_Score=250, Evalue=2e-66,
Organism=Escherichia coli, GI1788620, Length=407, Percent_Identity=42.2604422604423, Blast_Score=315, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17531727, Length=420, Percent_Identity=36.6666666666667, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI19920794, Length=370, Percent_Identity=38.9189189189189, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI45550452, Length=367, Percent_Identity=37.3297002724796, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24653819, Length=361, Percent_Identity=31.8559556786704, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=29.8192771084337, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55203; Mature: 55072

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAVTTIFVPRDSTALALGADDVARAIAREAAARGESVHIVRNGSRGMFWLEPLVEVQT
CCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHH
EAGRAAYGPVSVDDVPGLLDAGLLQGGEHVLAHGLTDEIPFLKRQERLTFARVGITDPLS
HCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCC
LDDYRAHEGYAGLERALAMEPAAIVQEVTDSGLRGRGGAAFPTGIKWKTVLGAKSAVKYI
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHCCCCCEEEE
VCNADEGDSGTFSDRMVMEDDPFVLIEGMTIAGLAVGAEYGYIYCRSEYPHAIAVLNAAI
EEECCCCCCCCCCCCEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCCHHHHHHHHH
ATATAAGWLGDDIRGSGKRFHLEVRKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALE
HHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHH
GLFGKPTVINNVISLATVPVILARGAQHYRDFGMGRSRGTLPFQLAGNIKQGGLVEKAFG
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHH
VTLRELLYDYGGGTRSGRAVRAVQVGGPLGAYLPEARFDVPMDYEAYAAFGGVVGHGGIV
HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHCCEECCCCEE
VFDETVDMARQARYAMEFCAIESCGKCTPCRIGSTRGVEVMDRIIAGNEPVKHVKLVRDL
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
CDTMLNGSLCAMGGMTPYPVLSALNEFPEDFGLASNPAKAA
HHHHHCCCEEECCCCCHHHHHHHHHHCHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAAVTTIFVPRDSTALALGADDVARAIAREAAARGESVHIVRNGSRGMFWLEPLVEVQT
CCCEEEEEEECCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHHHHH
EAGRAAYGPVSVDDVPGLLDAGLLQGGEHVLAHGLTDEIPFLKRQERLTFARVGITDPLS
HCCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCC
LDDYRAHEGYAGLERALAMEPAAIVQEVTDSGLRGRGGAAFPTGIKWKTVLGAKSAVKYI
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHCCCCCEEEE
VCNADEGDSGTFSDRMVMEDDPFVLIEGMTIAGLAVGAEYGYIYCRSEYPHAIAVLNAAI
EEECCCCCCCCCCCCEEECCCCEEEEECCEEEEEEEECCCCEEEEECCCCCHHHHHHHHH
ATATAAGWLGDDIRGSGKRFHLEVRKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALE
HHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHH
GLFGKPTVINNVISLATVPVILARGAQHYRDFGMGRSRGTLPFQLAGNIKQGGLVEKAFG
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHH
VTLRELLYDYGGGTRSGRAVRAVQVGGPLGAYLPEARFDVPMDYEAYAAFGGVVGHGGIV
HHHHHHHHHCCCCCCCCCEEEEEEECCCHHHCCCCCCCCCCCCHHHHHHHCCEECCCCEE
VFDETVDMARQARYAMEFCAIESCGKCTPCRIGSTRGVEVMDRIIAGNEPVKHVKLVRDL
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
CDTMLNGSLCAMGGMTPYPVLSALNEFPEDFGLASNPAKAA
HHHHHCCCEEECCCCCHHHHHHHHHHCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]