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The map label for this gene is nuoE2 [H]

Identifier: 73540323

GI number: 73540323

Start: 663759

End: 664298

Strand: Direct

Name: nuoE2 [H]

Synonym: Reut_A0617

Alternate gene names: 73540323

Gene position: 663759-664298 (Clockwise)

Preceding gene: 73540321

Following gene: 73540324

Centisome position: 17.44

GC content: 69.63

Gene sequence:

>540_bases
ATGCCAGATACCGCCACTTCCGCTCCGGCGCCGGCCGACGCGGCCCGCACAGCGGCCATCGCCGCGATCGTGGCGGCGCG
CCAGGATATGCCGGGCGCCTTGCTGCCCATCCTGCACGAAATCCAGGACACCCACGGCTACATCCCCGACACGGCCGTGC
CGGTCATCGCCAAGGCGCTGAACCTGTCACGTGCCGAGGTGCATGGCGTGATCACGTTCTACCACCATTTCCGCCAGCAG
CCGGCCGGCCGCCACGTGGTGCAGGTCTGCCGTGCCGAGGCCTGCCAGTCGGTCGGCGCCGAGGCGCTGGCCGAGCACGC
GAAGCGCGCACTCGGTTGCGACTTCCATGAAACCACCGCCGACGGTGCCGTCACGCTGGAGCCGGTCTATTGCCTCGGCC
AGTGCGCCTGCGGCCCGGCCGTGATGATGGGCGAGCAACTGCACGGCTATGTCGACGCAAAGCGCTTCGACGTGCTGGTG
CGCAAGCTGCGCGCCGACCAGACCGCCCACCAGCCCGCCAATCAGGAGACCCAGGCATGA

Upstream 100 bases:

>100_bases
GCTGCAACGGCCATAAAGCCGTGGTTATACCCCGGTAAATAGGAAAAAAATAGCATATAAACGGTCGCCCTGCAGAGGGC
GCGGCCCCACAGGAGACGCC

Downstream 100 bases:

>100_bases
GCGCCGCCGTCACCACCATCTTTGTCCCGCGCGATTCCACCGCGCTGGCCCTTGGCGCCGACGACGTGGCGCGCGCGATT
GCTCGTGAAGCGGCTGCGCG

Product: NADH dehydrogenase (ubiquinone), 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MPDTATSAPAPADAARTAAIAAIVAARQDMPGALLPILHEIQDTHGYIPDTAVPVIAKALNLSRAEVHGVITFYHHFRQQ
PAGRHVVQVCRAEACQSVGAEALAEHAKRALGCDFHETTADGAVTLEPVYCLGQCACGPAVMMGEQLHGYVDAKRFDVLV
RKLRADQTAHQPANQETQA

Sequences:

>Translated_179_residues
MPDTATSAPAPADAARTAAIAAIVAARQDMPGALLPILHEIQDTHGYIPDTAVPVIAKALNLSRAEVHGVITFYHHFRQQ
PAGRHVVQVCRAEACQSVGAEALAEHAKRALGCDFHETTADGAVTLEPVYCLGQCACGPAVMMGEQLHGYVDAKRFDVLV
RKLRADQTAHQPANQETQA
>Mature_178_residues
PDTATSAPAPADAARTAAIAAIVAARQDMPGALLPILHEIQDTHGYIPDTAVPVIAKALNLSRAEVHGVITFYHHFRQQP
AGRHVVQVCRAEACQSVGAEALAEHAKRALGCDFHETTADGAVTLEPVYCLGQCACGPAVMMGEQLHGYVDAKRFDVLVR
KLRADQTAHQPANQETQA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=135, Percent_Identity=30.3703703703704, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1788621, Length=151, Percent_Identity=31.7880794701987, Blast_Score=87, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17561328, Length=134, Percent_Identity=35.8208955223881, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18859877, Length=138, Percent_Identity=31.1594202898551, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355353, Length=136, Percent_Identity=30.1470588235294, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19051; Mature: 18920

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTATSAPAPADAARTAAIAAIVAARQDMPGALLPILHEIQDTHGYIPDTAVPVIAKAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NLSRAEVHGVITFYHHFRQQPAGRHVVQVCRAEACQSVGAEALAEHAKRALGCDFHETTA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
DGAVTLEPVYCLGQCACGPAVMMGEQLHGYVDAKRFDVLVRKLRADQTAHQPANQETQA
CCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PDTATSAPAPADAARTAAIAAIVAARQDMPGALLPILHEIQDTHGYIPDTAVPVIAKAL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NLSRAEVHGVITFYHHFRQQPAGRHVVQVCRAEACQSVGAEALAEHAKRALGCDFHETTA
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
DGAVTLEPVYCLGQCACGPAVMMGEQLHGYVDAKRFDVLVRKLRADQTAHQPANQETQA
CCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]