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The map label for this gene is fliM [H]

Identifier: 73539704

GI number: 73539704

Start: 2724015

End: 2725028

Strand: Reverse

Name: fliM [H]

Synonym: Reut_B5884

Alternate gene names: 73539704

Gene position: 2725028-2724015 (Counterclockwise)

Preceding gene: 73539705

Following gene: 73539703

Centisome position: 99.96

GC content: 62.72

Gene sequence:

>1014_bases
ATGGCCTATGACAAGTTCCTCTCGCAGGACGAGGTAGACGAGCTGCTCAAGGGCGTTTCCGGCGAAGCGGACACGCCCAA
GGCGAGCGAGCCCGCCGCGCCCGACGAGGGCGGCGTGCGCCCGTACAACCTCGCTACGCAGGAACGCGTGATCCGCGGGC
GCCTGCACACGCTCGAGATCATCAACGAGCGCTTTGCGCGTTCGCTGCGTACTGCACTGTTCAATTTCATCCGCCGTGGC
GCGGACATTACCGTGGGGAGCGTGCGCATCGAGAAGTTCGGCGATTTCGCGCGCAACCTGCCGATGCCGACCAACCTGAA
CCTGGTCCACATGAAGCCGCTGCGTGGCACGGCACTATTCGTGTACGACCCGAACCTGGTGTTCCTGGTGGTCGACAACC
TGTTCGGAGGCGACGGCCGCTTCCACACCCGCGTGGAAGGCCGTGACTTCACCCAGACCGAGCAGCGCATTATCCAGCGC
ATGCTGGAGATGACGCTGACCAGCTACGGCAATGCCTGGCGCACCGTGTATCCGATCGAGACCGAATACGTCCGCTCGGA
GATGCACACCAAGTTTGCAAACGTCGCCACGGTCAATGAGGCCGTGATCACCACCGCCTTCCACATCGAACTCGGCGCCG
TGGGCGGACAGCTGCATGTCTGCCTGCCCTACGCCATGATCGAGCCGCTCAAGGACCTGCTGATGAATCCGCTGCAGGAC
GAGAAGGAGGTCGACAAGAGCTGGCTCGCGAAACTGTCCACGCAGTTGCGCGCGGCCGATGTGGAAGTCGTGGCCGAGTT
CACGCACGTGCAAAGCACCGTCGCGGAAGTCCTGGCGCTGCGCGCCGGCGACGTGCTGCCGATCGAGCTGCCGGAGCACG
TGTTCGGCAAGGTGAACGGCGTGCCCGTCATGCAATGCGGATTCGGCACGATCAATGGCCAGTACGCGCTGCGGGTAGAG
CGAATGATCAATCACCAAGACAGCGATTCCAACAAGGAAACCGATCATGACTGA

Upstream 100 bases:

>100_bases
CAACGCCACCACCGCACCCGCATGCTTGCGGGCCGCCGTGCGCGCTGACGCGGATATGGACAGTTCGATTCACACCGCAG
CATAAGGGGCAGGACACCAG

Downstream 100 bases:

>100_bases
CGGCATCGAGGACAAGCCGGTCGATCCGATGGACGACTGGGCCAGCGCGCTGGCGGAACAGACCAGCGCCACTGCGGCCG
AAGCCCCGGTAGCGGCCGCC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAYDKFLSQDEVDELLKGVSGEADTPKASEPAAPDEGGVRPYNLATQERVIRGRLHTLEIINERFARSLRTALFNFIRRG
ADITVGSVRIEKFGDFARNLPMPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIQR
MLEMTLTSYGNAWRTVYPIETEYVRSEMHTKFANVATVNEAVITTAFHIELGAVGGQLHVCLPYAMIEPLKDLLMNPLQD
EKEVDKSWLAKLSTQLRAADVEVVAEFTHVQSTVAEVLALRAGDVLPIELPEHVFGKVNGVPVMQCGFGTINGQYALRVE
RMINHQDSDSNKETDHD

Sequences:

>Translated_337_residues
MAYDKFLSQDEVDELLKGVSGEADTPKASEPAAPDEGGVRPYNLATQERVIRGRLHTLEIINERFARSLRTALFNFIRRG
ADITVGSVRIEKFGDFARNLPMPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIQR
MLEMTLTSYGNAWRTVYPIETEYVRSEMHTKFANVATVNEAVITTAFHIELGAVGGQLHVCLPYAMIEPLKDLLMNPLQD
EKEVDKSWLAKLSTQLRAADVEVVAEFTHVQSTVAEVLALRAGDVLPIELPEHVFGKVNGVPVMQCGFGTINGQYALRVE
RMINHQDSDSNKETDHD
>Mature_336_residues
AYDKFLSQDEVDELLKGVSGEADTPKASEPAAPDEGGVRPYNLATQERVIRGRLHTLEIINERFARSLRTALFNFIRRGA
DITVGSVRIEKFGDFARNLPMPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIQRM
LEMTLTSYGNAWRTVYPIETEYVRSEMHTKFANVATVNEAVITTAFHIELGAVGGQLHVCLPYAMIEPLKDLLMNPLQDE
KEVDKSWLAKLSTQLRAADVEVVAEFTHVQSTVAEVLALRAGDVLPIELPEHVFGKVNGVPVMQCGFGTINGQYALRVER
MINHQDSDSNKETDHD

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=332, Percent_Identity=52.4096385542169, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37814; Mature: 37682

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDKFLSQDEVDELLKGVSGEADTPKASEPAAPDEGGVRPYNLATQERVIRGRLHTLEI
CCCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
INERFARSLRTALFNFIRRGADITVGSVRIEKFGDFARNLPMPTNLNLVHMKPLRGTALF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCCCCEEEEECCCCCCEEE
VYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIQRMLEMTLTSYGNAWRTVYPIE
EECCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
TEYVRSEMHTKFANVATVNEAVITTAFHIELGAVGGQLHVCLPYAMIEPLKDLLMNPLQD
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCC
EKEVDKSWLAKLSTQLRAADVEVVAEFTHVQSTVAEVLALRAGDVLPIELPEHVFGKVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCC
VPVMQCGFGTINGQYALRVERMINHQDSDSNKETDHD
CEEEEECCCCCCCHHEEHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYDKFLSQDEVDELLKGVSGEADTPKASEPAAPDEGGVRPYNLATQERVIRGRLHTLEI
CCHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
INERFARSLRTALFNFIRRGADITVGSVRIEKFGDFARNLPMPTNLNLVHMKPLRGTALF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHCCCCCCCCEEEEECCCCCCEEE
VYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIQRMLEMTLTSYGNAWRTVYPIE
EECCCEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCH
TEYVRSEMHTKFANVATVNEAVITTAFHIELGAVGGQLHVCLPYAMIEPLKDLLMNPLQD
HHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCC
EKEVDKSWLAKLSTQLRAADVEVVAEFTHVQSTVAEVLALRAGDVLPIELPEHVFGKVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCC
VPVMQCGFGTINGQYALRVERMINHQDSDSNKETDHD
CEEEEECCCCCCCHHEEHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]