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The map label for this gene is fliN [H]

Identifier: 73539703

GI number: 73539703

Start: 2723594

End: 2724022

Strand: Reverse

Name: fliN [H]

Synonym: Reut_B5883

Alternate gene names: 73539703

Gene position: 2724022-2723594 (Counterclockwise)

Preceding gene: 73539704

Following gene: 73539702

Centisome position: 99.92

GC content: 63.64

Gene sequence:

>429_bases
ATGACTGACGGCATCGAGGACAAGCCGGTCGATCCGATGGACGACTGGGCCAGCGCGCTGGCGGAACAGACCAGCGCCAC
TGCGGCCGAAGCCCCGGTAGCGGCCGCCATCCCGGCCGCCGCCACCGTTTTCCCGCCGCTGGCCAAGGAAGCACCGAGCG
GCTTCCAGAACGATATCGAGATGATCCTCGATATCCCGGTACAGCTCACGGTCGAGCTTGGCCGCACCAAGGTGCCCATC
AAGAACCTGCTACAGCTTGCGCAGGGATCCGTGGTGGAACTGGACGGCCTGGCCGGCGAGCCGATGGACGTGCTGGTCAA
CGGCTACCTGATTGCGCAGGGTGAGGTGGTGGTGGTGAACGACAAGTTTGGCATTCGCCTGACGGACATCATCACGCCGT
CCGAACGCATCCGCAAGCTCAACAAATGA

Upstream 100 bases:

>100_bases
GTCATGCAATGCGGATTCGGCACGATCAATGGCCAGTACGCGCTGCGGGTAGAGCGAATGATCAATCACCAAGACAGCGA
TTCCAACAAGGAAACCGATC

Downstream 100 bases:

>100_bases
ACGCCGTCGGCCGCCTGCGGCGCGCCGCCCTGCCGCTCGCGGCGGTGGCGGCGCCGTGCCTGGCGCATGCCGCCGAAGGC
AACGGGCCCGCCATCAGCGG

Product: Type III secretion system outer membrane O protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MTDGIEDKPVDPMDDWASALAEQTSATAAEAPVAAAIPAAATVFPPLAKEAPSGFQNDIEMILDIPVQLTVELGRTKVPI
KNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK

Sequences:

>Translated_142_residues
MTDGIEDKPVDPMDDWASALAEQTSATAAEAPVAAAIPAAATVFPPLAKEAPSGFQNDIEMILDIPVQLTVELGRTKVPI
KNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK
>Mature_141_residues
TDGIEDKPVDPMDDWASALAEQTSATAAEAPVAAAIPAAATVFPPLAKEAPSGFQNDIEMILDIPVQLTVELGRTKVPIK
NLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVNDKFGIRLTDIITPSERIRKLNK

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=131, Percent_Identity=61.0687022900763, Blast_Score=157, Evalue=2e-40,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 15075; Mature: 14943

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGIEDKPVDPMDDWASALAEQTSATAAEAPVAAAIPAAATVFPPLAKEAPSGFQNDIE
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCHHHCCCHHHHCCCHHHHCCCCCHHHHH
MILDIPVQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVN
HHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHEEECEEEECCCEEEEE
DKFGIRLTDIITPSERIRKLNK
CCCCEEEEEEECCHHHHHHCCC
>Mature Secondary Structure 
TDGIEDKPVDPMDDWASALAEQTSATAAEAPVAAAIPAAATVFPPLAKEAPSGFQNDIE
CCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCHHHCCCHHHHCCCHHHHCCCCCHHHHH
MILDIPVQLTVELGRTKVPIKNLLQLAQGSVVELDGLAGEPMDVLVNGYLIAQGEVVVVN
HHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCHHHEEECEEEECCCEEEEE
DKFGIRLTDIITPSERIRKLNK
CCCCEEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]