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The map label for this gene is citA [H]

Identifier: 73539633

GI number: 73539633

Start: 2637963

End: 2639285

Strand: Direct

Name: citA [H]

Synonym: Reut_B5813

Alternate gene names: 73539633

Gene position: 2637963-2639285 (Clockwise)

Preceding gene: 73539632

Following gene: 73539634

Centisome position: 96.77

GC content: 67.35

Gene sequence:

>1323_bases
ATGGAGCAAAGACTCGATGCGGCCCTGACGGGCCCTGTCTTACCTGGCTCGCCGACCGCGCGGGCTGACGCCACAACATT
CGCCAGGCAGCGCCGCGCCATAGTGGCGGCCTCGCTCGGCAACGCGCTGGAGTTGTTCGACTTCACGGTGTTCAGCTTCT
TCGCCGGGCTGATTGGCAAGTTGTTCTTTCCGGTAGAAGGCGAATATGGCCCGTTGCTGCTGGCGCTGGCGACGTTCGGC
ATCGGCTTTGTCATGCGGCCCGTGGGCAGCGTGGTGATCGGCAACTACGCCGACCGCGCCGGTCGCAAGCCGGCCTTGAC
GCTGACGATCGGCCTGATGGCGCTCGGCACGGCCATCATCGGTCTGGCGCCCACCTATGCACAGATCGGCATCGCCGCGC
CGCTGCTGGTCGTCACGGGCCGGCTGCTACAGGGCTTCTCGGCCGGCGGCGAGATCGGCGCCGCTACCAGCTTCCTGATG
GAGTCCGGCACGCGCAGCAACCGCGGCTTCATGGTGAGCTGGCAGATGGCCGGGCAGGGTGCGTCGGCGCTGGCCGGCGC
CCTGTGCGGTGCCCTGCTGATCCACGGCCTGCCGCAGGCCGCGCTGGAAAGCTGGGGCTGGCGTGTGCCCTTTCTGCTCG
GCCTGCTTATTGGCCCGGTCGGCTTCTATATCCGCCGCCACCTCGACGAGACTCACCAGGACGTAGCGCGCGAGCGCAGC
GCGCTGTCTGAGGCGGTGCAACAGCACGGCCGCAAGCTGCTGCTCGGCACGCTGCTGATCCTCGGCGGCACTGCCGAAAT
GTACGTAGTGGTGTTCTTCACGCCTGCCTGGCTGGTCAAGACCTTCCACATGCCGGCCGCCACGTCGCTGCTCACCGGTT
GCGCGGCCGGGGCGATCATGCTCCTGGTATCGCCGCTGGCGGGCGTGCTCGCTGACCGCCTGGGCCGCCGCAAGCCGTTG
CTGCTGACGCTGCGCATGGTGTCGCTGCTAGGGATACTGCCGGGATTCCTGCTGGTGCAGAATGCGCCGTCACTCGGGTC
GGCACTGGTGCTGGTGTGCGGTGTCATGATGCTGGCTACGCTGGGGGCGCCGGCCGGCTTCCTATTCATCATGGAAAGCT
TCCCGCGCCGCGTGCGTGCCACCGGGCTGGCGATCATCTATGCCTGCGGCGTGACGCTGTTCGGAGGCTTTGCTCAGTTT
ATCGTCACGTGGATGCTGAGCGTGACAGGGAACCCGCTTTCACCCGCGTGGTATGTGCTCGGCTGCGGCACGGCGAGCCT
GCTGGCGCTGCTGGCGTTTGCTGAGCAGCGTGTCGCGGACTGA

Upstream 100 bases:

>100_bases
GGCTACGATTTCAACGACGAGGCGCTGACGCTGGGCGCAAGCATGTGGGCGCGGCTGGTTGAGTGCTACCTCGTCGCCGC
CTGAAAAGGGAGGATGCTGT

Downstream 100 bases:

>100_bases
TTAGGGCGATAACGCCATCCAGCGCTCAAGTTCATCCAACCGGCAGATGAATCCGCTTGGAGCATTCGGTCGCCCTCAAT
TGCGGTAGAGTGGGCGACCT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MEQRLDAALTGPVLPGSPTARADATTFARQRRAIVAASLGNALELFDFTVFSFFAGLIGKLFFPVEGEYGPLLLALATFG
IGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAIIGLAPTYAQIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLM
ESGTRSNRGFMVSWQMAGQGASALAGALCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERS
ALSEAVQQHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIMLLVSPLAGVLADRLGRRKPL
LLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLATLGAPAGFLFIMESFPRRVRATGLAIIYACGVTLFGGFAQF
IVTWMLSVTGNPLSPAWYVLGCGTASLLALLAFAEQRVAD

Sequences:

>Translated_440_residues
MEQRLDAALTGPVLPGSPTARADATTFARQRRAIVAASLGNALELFDFTVFSFFAGLIGKLFFPVEGEYGPLLLALATFG
IGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAIIGLAPTYAQIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLM
ESGTRSNRGFMVSWQMAGQGASALAGALCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERS
ALSEAVQQHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIMLLVSPLAGVLADRLGRRKPL
LLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLATLGAPAGFLFIMESFPRRVRATGLAIIYACGVTLFGGFAQF
IVTWMLSVTGNPLSPAWYVLGCGTASLLALLAFAEQRVAD
>Mature_440_residues
MEQRLDAALTGPVLPGSPTARADATTFARQRRAIVAASLGNALELFDFTVFSFFAGLIGKLFFPVEGEYGPLLLALATFG
IGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAIIGLAPTYAQIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLM
ESGTRSNRGFMVSWQMAGQGASALAGALCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERS
ALSEAVQQHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIMLLVSPLAGVLADRLGRRKPL
LLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLATLGAPAGFLFIMESFPRRVRATGLAIIYACGVTLFGGFAQF
IVTWMLSVTGNPLSPAWYVLGCGTASLLALLAFAEQRVAD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=441, Percent_Identity=33.1065759637188, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1788942, Length=425, Percent_Identity=33.6470588235294, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788292, Length=436, Percent_Identity=30.7339449541284, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789941, Length=404, Percent_Identity=30.9405940594059, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082404, Length=372, Percent_Identity=24.4623655913978, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082231, Length=217, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46177; Mature: 46177

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQRLDAALTGPVLPGSPTARADATTFARQRRAIVAASLGNALELFDFTVFSFFAGLIGK
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LFFPVEGEYGPLLLALATFGIGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAII
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHHHHHHHHHHH
GLAPTYAQIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLMESGTRSNRGFMVSWQMAGQG
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC
ASALAGALCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERS
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSEAVQQHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIM
HHHHHHHHHCHHHHHHHHHHHCCCHHEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHH
LLVSPLAGVLADRLGRRKPLLLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLAT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LGAPAGFLFIMESFPRRVRATGLAIIYACGVTLFGGFAQFIVTWMLSVTGNPLSPAWYVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
GCGTASLLALLAFAEQRVAD
ECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQRLDAALTGPVLPGSPTARADATTFARQRRAIVAASLGNALELFDFTVFSFFAGLIGK
CCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LFFPVEGEYGPLLLALATFGIGFVMRPVGSVVIGNYADRAGRKPALTLTIGLMALGTAII
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEHHHHHHHHHHHHHH
GLAPTYAQIGIAAPLLVVTGRLLQGFSAGGEIGAATSFLMESGTRSNRGFMVSWQMAGQG
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCC
ASALAGALCGALLIHGLPQAALESWGWRVPFLLGLLIGPVGFYIRRHLDETHQDVARERS
HHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALSEAVQQHGRKLLLGTLLILGGTAEMYVVVFFTPAWLVKTFHMPAATSLLTGCAAGAIM
HHHHHHHHHCHHHHHHHHHHHCCCHHEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHH
LLVSPLAGVLADRLGRRKPLLLTLRMVSLLGILPGFLLVQNAPSLGSALVLVCGVMMLAT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LGAPAGFLFIMESFPRRVRATGLAIIYACGVTLFGGFAQFIVTWMLSVTGNPLSPAWYVL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
GCGTASLLALLAFAEQRVAD
ECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]