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The map label for this gene is hipO [H]

Identifier: 73539632

GI number: 73539632

Start: 2636762

End: 2637946

Strand: Direct

Name: hipO [H]

Synonym: Reut_B5812

Alternate gene names: 73539632

Gene position: 2636762-2637946 (Clockwise)

Preceding gene: 73539626

Following gene: 73539633

Centisome position: 96.72

GC content: 65.82

Gene sequence:

>1185_bases
ATGTCGCAAGGCGCCGCCATTATTCCCGAAATTCACGCCCATCAGGCGGACTTCGTCGCGCTGCGCCGCGATATCCACGC
ACACCCGGAACTCGGCTTCGAGGAAGTCCGCACCAGCCGCTTGGTGGCCGACAAGCTGGCGGAGTGGGGCTACGACGTGA
CGACAGGCGTGGGCGGAACCGGCGTGGTTGGGCAACTGAAGCTCGGCAACGGTACGCGCCGCATCGGCATCCGCGCCGAT
ATGGACGCGCTTCCCATCGTCGAGGCTACCGGCCTGCCGTACGCCAGTATCCACCACGGCAAAATGCACGCCTGCGGCCA
CGACGGCCACACCGCTATCCTGCTTGCCGCCGCGCACTACTTCGCCACCACGCGCCGGTTCGACGGCACACTCAACCTGA
TCTTTCAGCCGGCTGAGGAGGGGCAGGGCGGTGCGCAGCGCATGGTGGCGGATGGCCTGTTCAAGCGTTTCCCGTGCGAC
GCGGTTTACGCCCTGCACAATGCGCCCGGCGTGCCGCTCGGGCACTTCGTGGTGCATGAGGGGCCGACGCTGGCCTCGGC
CGATACCGTCACCATCACGCTGACCGGCAAAGGCGCGCACGGCGCCATGCCGCAGTACGGACGCGATCCCGTGGTGGCGG
CTGCCGCCATTGTCATGGCGCTGCAGACAATCGTCGCGCGCAACCTGTCGGCCACCGATGCCGGAGTGATTACTGTGGGT
GCACTGCAGGCCGGCACCGCGCACAACGTTATTCCCGAGACAGCGAAAATCCTCCTGACCGTGCGCGCACTCGACCGCAC
TGTGCGCGACACGCTCGAGGCCCGCCTGCGCGAAGTGGTCGACCTGCAGGCCCGCAGCCTCGGGACGAGTGCGGAAATTC
ACTATCACAAGGTCACCAGCGTGGTGGTCAATACGCCAGACGAAACGCGCTTCATCCGCGGTGTCATCCAGGACCTCGTC
GGCCCGGATTGCGTGCAGGACATGGAAAAGGGAGCAATGGGCAGCGAGGATTTCTCGTGGATGCTCGACGAAGTGCCAGG
CTGCTACGTTTTGCTCGGGAACGGTAGCGGCTCGCAGGGCGGCTGCATGGTCCACAACCCCGGCTACGATTTCAACGACG
AGGCGCTGACGCTGGGCGCAAGCATGTGGGCGCGGCTGGTTGAGTGCTACCTCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
AATAACCCCCGGTTATGGTTATCAACTTTCGTGCTTTGCTGCTGTGGCGTGGGATTCCCTACCATGGGCCTTCTGCATCC
ATCTCCACAGGAACCCGCAA

Downstream 100 bases:

>100_bases
AAAGGGAGGATGCTGTATGGAGCAAAGACTCGATGCGGCCCTGACGGGCCCTGTCTTACCTGGCTCGCCGACCGCGCGGG
CTGACGCCACAACATTCGCC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSQGAAIIPEIHAHQADFVALRRDIHAHPELGFEEVRTSRLVADKLAEWGYDVTTGVGGTGVVGQLKLGNGTRRIGIRAD
MDALPIVEATGLPYASIHHGKMHACGHDGHTAILLAAAHYFATTRRFDGTLNLIFQPAEEGQGGAQRMVADGLFKRFPCD
AVYALHNAPGVPLGHFVVHEGPTLASADTVTITLTGKGAHGAMPQYGRDPVVAAAAIVMALQTIVARNLSATDAGVITVG
ALQAGTAHNVIPETAKILLTVRALDRTVRDTLEARLREVVDLQARSLGTSAEIHYHKVTSVVVNTPDETRFIRGVIQDLV
GPDCVQDMEKGAMGSEDFSWMLDEVPGCYVLLGNGSGSQGGCMVHNPGYDFNDEALTLGASMWARLVECYLVAA

Sequences:

>Translated_394_residues
MSQGAAIIPEIHAHQADFVALRRDIHAHPELGFEEVRTSRLVADKLAEWGYDVTTGVGGTGVVGQLKLGNGTRRIGIRAD
MDALPIVEATGLPYASIHHGKMHACGHDGHTAILLAAAHYFATTRRFDGTLNLIFQPAEEGQGGAQRMVADGLFKRFPCD
AVYALHNAPGVPLGHFVVHEGPTLASADTVTITLTGKGAHGAMPQYGRDPVVAAAAIVMALQTIVARNLSATDAGVITVG
ALQAGTAHNVIPETAKILLTVRALDRTVRDTLEARLREVVDLQARSLGTSAEIHYHKVTSVVVNTPDETRFIRGVIQDLV
GPDCVQDMEKGAMGSEDFSWMLDEVPGCYVLLGNGSGSQGGCMVHNPGYDFNDEALTLGASMWARLVECYLVAA
>Mature_393_residues
SQGAAIIPEIHAHQADFVALRRDIHAHPELGFEEVRTSRLVADKLAEWGYDVTTGVGGTGVVGQLKLGNGTRRIGIRADM
DALPIVEATGLPYASIHHGKMHACGHDGHTAILLAAAHYFATTRRFDGTLNLIFQPAEEGQGGAQRMVADGLFKRFPCDA
VYALHNAPGVPLGHFVVHEGPTLASADTVTITLTGKGAHGAMPQYGRDPVVAAAAIVMALQTIVARNLSATDAGVITVGA
LQAGTAHNVIPETAKILLTVRALDRTVRDTLEARLREVVDLQARSLGTSAEIHYHKVTSVVVNTPDETRFIRGVIQDLVG
PDCVQDMEKGAMGSEDFSWMLDEVPGCYVLLGNGSGSQGGCMVHNPGYDFNDEALTLGASMWARLVECYLVAA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=420, Percent_Identity=29.2857142857143, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41867; Mature: 41736

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGAAIIPEIHAHQADFVALRRDIHAHPELGFEEVRTSRLVADKLAEWGYDVTTGVGGT
CCCCCEECCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCC
GVVGQLKLGNGTRRIGIRADMDALPIVEATGLPYASIHHGKMHACGHDGHTAILLAAAHY
CCEEEEEECCCCEEEEEEECCCCCCEEEECCCCCEEECCCCEEECCCCCCEEEHHHHHHH
FATTRRFDGTLNLIFQPAEEGQGGAQRMVADGLFKRFPCDAVYALHNAPGVPLGHFVVHE
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEC
GPTLASADTVTITLTGKGAHGAMPQYGRDPVVAAAAIVMALQTIVARNLSATDAGVITVG
CCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
ALQAGTAHNVIPETAKILLTVRALDRTVRDTLEARLREVVDLQARSLGTSAEIHYHKVTS
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
VVVNTPDETRFIRGVIQDLVGPDCVQDMEKGAMGSEDFSWMLDEVPGCYVLLGNGSGSQG
EEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCC
GCMVHNPGYDFNDEALTLGASMWARLVECYLVAA
CEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQGAAIIPEIHAHQADFVALRRDIHAHPELGFEEVRTSRLVADKLAEWGYDVTTGVGGT
CCCCEECCHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCC
GVVGQLKLGNGTRRIGIRADMDALPIVEATGLPYASIHHGKMHACGHDGHTAILLAAAHY
CCEEEEEECCCCEEEEEEECCCCCCEEEECCCCCEEECCCCEEECCCCCCEEEHHHHHHH
FATTRRFDGTLNLIFQPAEEGQGGAQRMVADGLFKRFPCDAVYALHNAPGVPLGHFVVHE
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEC
GPTLASADTVTITLTGKGAHGAMPQYGRDPVVAAAAIVMALQTIVARNLSATDAGVITVG
CCCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
ALQAGTAHNVIPETAKILLTVRALDRTVRDTLEARLREVVDLQARSLGTSAEIHYHKVTS
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEE
VVVNTPDETRFIRGVIQDLVGPDCVQDMEKGAMGSEDFSWMLDEVPGCYVLLGNGSGSQG
EEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEECCCCCCC
GCMVHNPGYDFNDEALTLGASMWARLVECYLVAA
CEEEECCCCCCCCCCEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]