Click here to switch to the map view.

The map label for this gene is maiA [H]

Identifier: 73539624

GI number: 73539624

Start: 2627478

End: 2628119

Strand: Direct

Name: maiA [H]

Synonym: Reut_B5804

Alternate gene names: 73539624

Gene position: 2627478-2628119 (Clockwise)

Preceding gene: 73539623

Following gene: 73539625

Centisome position: 96.38

GC content: 64.33

Gene sequence:

>642_bases
ATGCAGCTCTACAGTTTTTTCAATAGTTCCACGTCGTACCGCGTGCGCATCGCCCTCGCGCTCAAGGGCCTGTCGTACGA
GTACCTGCCGGTGAACATCCGCGTCGGCGAACATCACGACGGGGATTACGTGAACGCAATCAACCCGTCGGCATCGGTAC
CGGCGCTGATCGATGGCGACTTCGCGCTCGGGCAGTCGCTGGCGATCGTGGATTATCTCGACGCGCGCCATCCGGAGCCG
CGCCTGCTGCCGACGAGCGGCCATCTCCGCGCGCGGGTGCTCGAACTGGCATATCTGGTGAGCTGCGACATGCACCCGGT
GAACAACCTGCGCATCCTCAAGTATCTGCAAAGCGAGCTGAAGGTGTCTCCCGAGCAGAAGGACGCGTGGTATCGCCACT
GGATCGATGAAGGCATGGCCGGCGTCGAGCGCCTGCTTTCCCGGTATGGGGAAGGGCCGTGGTGCTTCGGCTCCCAGCCC
ACGCTGGCAGACGTTTGCCTCGTGCCGCAGATCGCCAATGCCTTACGCATGGGCTGCGATCTTTCCGGTTACCCGAAGGC
GATGGCAGTGTATGCCCACGCCAGCGCGCACCCCGCTTTCGCCCTGGCCGCCCCGGCGCGGCAGCCCGACTACACCGCCT
GA

Upstream 100 bases:

>100_bases
CGTGGGTGCGGTAAAGGCCGGCGAGACGATGGTGACGGGCGTGGACGGGCTCGGCGAACTGACCGTGCGCGTGGTCTGAC
GGAAATTCACGGGAGTCGAC

Downstream 100 bases:

>100_bases
GCGCAAGAGCGCCCGGGCATAGGAGACGACACATGCAATCGAATCAATCCGACAGGAAAGTCTTGATCATCGGCGGCGGC
ATCGGCGGTCTTGCCGCGGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQLYSFFNSSTSYRVRIALALKGLSYEYLPVNIRVGEHHDGDYVNAINPSASVPALIDGDFALGQSLAIVDYLDARHPEP
RLLPTSGHLRARVLELAYLVSCDMHPVNNLRILKYLQSELKVSPEQKDAWYRHWIDEGMAGVERLLSRYGEGPWCFGSQP
TLADVCLVPQIANALRMGCDLSGYPKAMAVYAHASAHPAFALAAPARQPDYTA

Sequences:

>Translated_213_residues
MQLYSFFNSSTSYRVRIALALKGLSYEYLPVNIRVGEHHDGDYVNAINPSASVPALIDGDFALGQSLAIVDYLDARHPEP
RLLPTSGHLRARVLELAYLVSCDMHPVNNLRILKYLQSELKVSPEQKDAWYRHWIDEGMAGVERLLSRYGEGPWCFGSQP
TLADVCLVPQIANALRMGCDLSGYPKAMAVYAHASAHPAFALAAPARQPDYTA
>Mature_213_residues
MQLYSFFNSSTSYRVRIALALKGLSYEYLPVNIRVGEHHDGDYVNAINPSASVPALIDGDFALGQSLAIVDYLDARHPEP
RLLPTSGHLRARVLELAYLVSCDMHPVNNLRILKYLQSELKVSPEQKDAWYRHWIDEGMAGVERLLSRYGEGPWCFGSQP
TLADVCLVPQIANALRMGCDLSGYPKAMAVYAHASAHPAFALAAPARQPDYTA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=40.9523809523809, Blast_Score=154, Evalue=5e-38,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=37.5, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1789624, Length=183, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17510461, Length=208, Percent_Identity=34.6153846153846, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=35.4066985645933, Blast_Score=121, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17551302, Length=213, Percent_Identity=35.2112676056338, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=39.8104265402844, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=39.8104265402844, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=39.8104265402844, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=36.0189573459716, Blast_Score=137, Evalue=5e-33,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23589; Mature: 23589

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLSYEYLPVNIRVGEHHDGDYVNAINPSASVPALIDGD
CCCEEECCCCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCEEEECCCCCCCCEEECCC
FALGQSLAIVDYLDARHPEPRLLPTSGHLRARVLELAYLVSCDMHPVNNLRILKYLQSEL
HHHCCCCCEEEHHHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KVSPEQKDAWYRHWIDEGMAGVERLLSRYGEGPWCFGSQPTLADVCLVPQIANALRMGCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LSGYPKAMAVYAHASAHPAFALAAPARQPDYTA
CCCCCCEEEEEEECCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MQLYSFFNSSTSYRVRIALALKGLSYEYLPVNIRVGEHHDGDYVNAINPSASVPALIDGD
CCCEEECCCCCCEEEEEEEEEECCCEEEEEEEEEECCCCCCCEEEECCCCCCCCEEECCC
FALGQSLAIVDYLDARHPEPRLLPTSGHLRARVLELAYLVSCDMHPVNNLRILKYLQSEL
HHHCCCCCEEEHHHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KVSPEQKDAWYRHWIDEGMAGVERLLSRYGEGPWCFGSQPTLADVCLVPQIANALRMGCD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
LSGYPKAMAVYAHASAHPAFALAAPARQPDYTA
CCCCCCEEEEEEECCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]