Click here to switch to the map view.

The map label for this gene is proP [H]

Identifier: 73539587

GI number: 73539587

Start: 2591905

End: 2593221

Strand: Direct

Name: proP [H]

Synonym: Reut_B5767

Alternate gene names: 73539587

Gene position: 2591905-2593221 (Clockwise)

Preceding gene: 73539586

Following gene: 73539588

Centisome position: 95.08

GC content: 61.12

Gene sequence:

>1317_bases
ATGACGTCAATCGACCTCGCGTTGCAAGCGCACCATCCCATGCTCAGGCAGAAGTGGAAAACCGTTATCCTGGCGAGCCT
TGGCGGTTCGCTGGAACTGTACGACTTCATCATCTACGGCGTCTTCGCGCGGGAGATCGCCACGCAATTCTTTCCCGCGA
CAGATCCCGTCGCTTCGCTGATTGGCGCCTTCGGCGTCTTCGCGCTCGGATACATGTCCCGGCCGCTGGGCGGTGTCATC
CTGAGCAGCCTGGGCGACCGATTGGGCCGGCGCGTGGTCTTCCTGGTCTCGGTGCTGGCGATGTCGGCCACGACCATCGG
CATTGGCCTGGTCCCCGGCTATGCGGCCATTGGCATGGCCGCACCGGTGTTGCTCGTGATGCTCCGCCTCCTGCAAGGAT
TCTTCCTGGCGGGGGAATTGCCCTGCTCGATCACGTATGTGGTAGAGGAGGTACCCGGCCGCGCGAGCACGGTCAGTGGG
CTTGTCATTTTCTGTGCCGCTGCGGGTATCCTGATCGCGACGCTCGTCAGCCTGGTCATTCATTCAACACTGTCGGCGGA
TGATGTCCTGACATACGGGTGGCGCATTGCCTTCCTTTTTGGCGGCGTCATCGGCCTCTTGTCGTACTGGCTGAGGACCT
CGCTCGAGGAGTCCAGCGAGTTCCAGCAACTCAGAAGCCACAAAGTCAAGCGCCCCTTCCGCCGTGTGCTCGCTGAATAT
CCGAAGCAAATCGTTGTTGGCGTGGGAGTGGCTGGGATCGTTAACGCGTCGAATAGCTTGTTGTTCGTGGTGCTGCCGTC
GTACATGGCGCTTGTACTGCATGCGGATCCGCGTCTTGTCAGCGAGGGGCAGAACATCGGGATTGCCACGATGTGCGTGT
CGCTGCTTCTCTTTGCCTGCCTGGGTGACCGCATAGCGTCTCGACACTGGCATCGGCTCGGGACAATCCTGATGCTGGCA
GGAAGCTACCCATTCTATGCACTGCTCACCGCCCAGAGGCTCGACGTGATCGCGGCCTTCACGATCATTGGGGCCATAGG
CGGACTGGTGAATGGGTCCTACGCCTTCCTGTTGGCGGATCTTTTCCCCACTGAAGTCCGCTTCAGTGGCATTGCGCTTT
CGCTTAACCTTGCCACGGTCATATTCACGGGCGCCACGCCCTTCCTGGTGACACAACTCCTGCACGCGACGGGATCTCCG
GCTGCGCCTGGCCTCTACCTGACGGGTGTCGCCGTTATTGCACTGGCTGCCGGCATCGGGCTGAAGGCTATGGGGGGCAG
AATCGATCGCGCCGGTACGATCGCGGGCGCAGCTTGA

Upstream 100 bases:

>100_bases
CTAGCCTGAGGGTAACGGTCGGACCTCGATGTTGGCCGTGCGCCACAAGTTCTTAGCGACGCGACGGAGAGCGCGGCACC
ATACCGATTGGAGACAAGTG

Downstream 100 bases:

>100_bases
TTCGCCACTAACGTCGGCCGGGCAGCAAGCGAAGTGGGCCACGGCTGCGTAGAACCGCTCATGTGTTCCTGTGTGGCTCC
CCAGGGCGATACAGGATCGA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTSIDLALQAHHPMLRQKWKTVILASLGGSLELYDFIIYGVFAREIATQFFPATDPVASLIGAFGVFALGYMSRPLGGVI
LSSLGDRLGRRVVFLVSVLAMSATTIGIGLVPGYAAIGMAAPVLLVMLRLLQGFFLAGELPCSITYVVEEVPGRASTVSG
LVIFCAAAGILIATLVSLVIHSTLSADDVLTYGWRIAFLFGGVIGLLSYWLRTSLEESSEFQQLRSHKVKRPFRRVLAEY
PKQIVVGVGVAGIVNASNSLLFVVLPSYMALVLHADPRLVSEGQNIGIATMCVSLLLFACLGDRIASRHWHRLGTILMLA
GSYPFYALLTAQRLDVIAAFTIIGAIGGLVNGSYAFLLADLFPTEVRFSGIALSLNLATVIFTGATPFLVTQLLHATGSP
AAPGLYLTGVAVIALAAGIGLKAMGGRIDRAGTIAGAA

Sequences:

>Translated_438_residues
MTSIDLALQAHHPMLRQKWKTVILASLGGSLELYDFIIYGVFAREIATQFFPATDPVASLIGAFGVFALGYMSRPLGGVI
LSSLGDRLGRRVVFLVSVLAMSATTIGIGLVPGYAAIGMAAPVLLVMLRLLQGFFLAGELPCSITYVVEEVPGRASTVSG
LVIFCAAAGILIATLVSLVIHSTLSADDVLTYGWRIAFLFGGVIGLLSYWLRTSLEESSEFQQLRSHKVKRPFRRVLAEY
PKQIVVGVGVAGIVNASNSLLFVVLPSYMALVLHADPRLVSEGQNIGIATMCVSLLLFACLGDRIASRHWHRLGTILMLA
GSYPFYALLTAQRLDVIAAFTIIGAIGGLVNGSYAFLLADLFPTEVRFSGIALSLNLATVIFTGATPFLVTQLLHATGSP
AAPGLYLTGVAVIALAAGIGLKAMGGRIDRAGTIAGAA
>Mature_437_residues
TSIDLALQAHHPMLRQKWKTVILASLGGSLELYDFIIYGVFAREIATQFFPATDPVASLIGAFGVFALGYMSRPLGGVIL
SSLGDRLGRRVVFLVSVLAMSATTIGIGLVPGYAAIGMAAPVLLVMLRLLQGFFLAGELPCSITYVVEEVPGRASTVSGL
VIFCAAAGILIATLVSLVIHSTLSADDVLTYGWRIAFLFGGVIGLLSYWLRTSLEESSEFQQLRSHKVKRPFRRVLAEYP
KQIVVGVGVAGIVNASNSLLFVVLPSYMALVLHADPRLVSEGQNIGIATMCVSLLLFACLGDRIASRHWHRLGTILMLAG
SYPFYALLTAQRLDVIAAFTIIGAIGGLVNGSYAFLLADLFPTEVRFSGIALSLNLATVIFTGATPFLVTQLLHATGSPA
APGLYLTGVAVIALAAGIGLKAMGGRIDRAGTIAGAA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=29.4252873563218, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=31.75, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=28.4722222222222, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=29.4392523364486, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082231, Length=235, Percent_Identity=28.936170212766, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87082404, Length=367, Percent_Identity=26.158038147139, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46242; Mature: 46111

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSIDLALQAHHPMLRQKWKTVILASLGGSLELYDFIIYGVFAREIATQFFPATDPVASL
CCHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH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HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]