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The map label for this gene is bbuR [H]

Identifier: 73539583

GI number: 73539583

Start: 2586866

End: 2587816

Strand: Direct

Name: bbuR [H]

Synonym: Reut_B5763

Alternate gene names: 73539583

Gene position: 2586866-2587816 (Clockwise)

Preceding gene: 73539582

Following gene: 73539584

Centisome position: 94.89

GC content: 64.77

Gene sequence:

>951_bases
ATGAACATCACGCTGCGTCAACTCAGGTACTTCGTGGAGATCGCGCAGAGCCGCAGCTTTTCGCGCGCCGCGGAGCGGTT
GCGGGTAGCCCAGCCCGCTCTCAGCCAGAATATCTCCTCACTGGAAGACAGCCTGGGCGCAAAGCTGTTCGAGCGCCATG
CTCGTGGCGTCGAAATCTCGCCGGCCGGTGAGCGGCTACTGACGAGCGCCATCGACATTCTCGCTCGTACCGACGCCTTG
AAGGACGACATCGACGGACGCGCAAGCCTTCCGACCGGGAGTGTGCGTCTGTCGATTGCCGGATCCATTGCCGGCGCCGT
CATTGCGCCGCTGCTTGGCGCCGTGGCACGCGAGTATCCCGGCATCGAAGTGACCGTGCGCGAAAGCATGTCCTTCGAAT
CGCGCGCGCTCGTCGAGTCGGGGCAGGCGCATCTGGCCCTCATGCCGAGCCCCTCGGAAATCCAGGGAATGGCCTCTATA
CCTCTCTACGAAGAGCGCTTCATGTTGTACGGCACGCGTGCCGCTATGCGCCGCAAGCCAGAAACGATGACATTCGCGGA
GGTGGCAGAACTGCCACTGGCCGCGCCGGATGGCGCGCATGACCTGCGCAAGATCATCGAGCGTGCGGCCAATGCCATCG
GCCGTTCCCTTGATGTCCGGTACGAACTCAACAGCCCGCAGATGCTGATCGCGCTGGCACGTGACGGACTCGCGTACGTT
GTGATGCCGCCCAGCGCCTGCCTCGAGGCCGTCGCGGCCAAATCCATTGTCGGACGCCCGGTGACGGAACCCGACCTGAC
CCGCATCCAGGCGCTGGTCTGGCCAGGGGACCGGCCCCTGTCTCCTGCTGCCGCGGCAATCCGTGACCTCATCGCCCACA
TTGTCGGTGACCTCGTGGAGCGCGGCGCGCTGCCCGGCCGGACGGTCGGGGCGACCTATAAGAAAAAATGA

Upstream 100 bases:

>100_bases
TACTTGCCTTTCTTCTCGTCAGTTCACCGCAATAAGTGCTTAAAATGGCACGCCAGAGGCTGGCGAAAGAGTACCGGCTG
ACACGGGAGGGACGGGCCAT

Downstream 100 bases:

>100_bases
AACCTTCCTAGTCCGAGACAGTATTGGTTTCGGCGCAGCGGGTTGATTAGAGTTGTCTCCAGAGCGCACTGAGACGCCTT
CGATGGAGACAAGCATGGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNITLRQLRYFVEIAQSRSFSRAAERLRVAQPALSQNISSLEDSLGAKLFERHARGVEISPAGERLLTSAIDILARTDAL
KDDIDGRASLPTGSVRLSIAGSIAGAVIAPLLGAVAREYPGIEVTVRESMSFESRALVESGQAHLALMPSPSEIQGMASI
PLYEERFMLYGTRAAMRRKPETMTFAEVAELPLAAPDGAHDLRKIIERAANAIGRSLDVRYELNSPQMLIALARDGLAYV
VMPPSACLEAVAAKSIVGRPVTEPDLTRIQALVWPGDRPLSPAAAAIRDLIAHIVGDLVERGALPGRTVGATYKKK

Sequences:

>Translated_316_residues
MNITLRQLRYFVEIAQSRSFSRAAERLRVAQPALSQNISSLEDSLGAKLFERHARGVEISPAGERLLTSAIDILARTDAL
KDDIDGRASLPTGSVRLSIAGSIAGAVIAPLLGAVAREYPGIEVTVRESMSFESRALVESGQAHLALMPSPSEIQGMASI
PLYEERFMLYGTRAAMRRKPETMTFAEVAELPLAAPDGAHDLRKIIERAANAIGRSLDVRYELNSPQMLIALARDGLAYV
VMPPSACLEAVAAKSIVGRPVTEPDLTRIQALVWPGDRPLSPAAAAIRDLIAHIVGDLVERGALPGRTVGATYKKK
>Mature_316_residues
MNITLRQLRYFVEIAQSRSFSRAAERLRVAQPALSQNISSLEDSLGAKLFERHARGVEISPAGERLLTSAIDILARTDAL
KDDIDGRASLPTGSVRLSIAGSIAGAVIAPLLGAVAREYPGIEVTVRESMSFESRALVESGQAHLALMPSPSEIQGMASI
PLYEERFMLYGTRAAMRRKPETMTFAEVAELPLAAPDGAHDLRKIIERAANAIGRSLDVRYELNSPQMLIALARDGLAYV
VMPPSACLEAVAAKSIVGRPVTEPDLTRIQALVWPGDRPLSPAAAAIRDLIAHIVGDLVERGALPGRTVGATYKKK

Specific function: Transcriptional Activator For The Hut, Put And Ure Operons And Repressor For The Gdh And Gltb Operons In Response To Nitrogen Limitation. Negative Regulator Of Its Own Expression (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=294, Percent_Identity=28.2312925170068, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367136, Length=276, Percent_Identity=28.9855072463768, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI145693105, Length=297, Percent_Identity=26.2626262626263, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=25.2559726962457, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790399, Length=284, Percent_Identity=26.056338028169, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=164, Percent_Identity=32.3170731707317, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787530, Length=281, Percent_Identity=24.5551601423488, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787601, Length=128, Percent_Identity=31.25, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790208, Length=163, Percent_Identity=28.8343558282209, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081904, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788887, Length=77, Percent_Identity=45.4545454545455, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=119, Percent_Identity=32.7731092436975, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=116, Percent_Identity=32.7586206896552, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789173, Length=123, Percent_Identity=34.1463414634146, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788706, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI145693193, Length=117, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789506, Length=171, Percent_Identity=24.5614035087719, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787806, Length=272, Percent_Identity=24.2647058823529, Blast_Score=61, Evalue=8e-11,
Organism=Escherichia coli, GI1786401, Length=126, Percent_Identity=30.952380952381, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34011; Mature: 34011

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITLRQLRYFVEIAQSRSFSRAAERLRVAQPALSQNISSLEDSLGAKLFERHARGVEIS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PAGERLLTSAIDILARTDALKDDIDGRASLPTGSVRLSIAGSIAGAVIAPLLGAVAREYP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
GIEVTVRESMSFESRALVESGQAHLALMPSPSEIQGMASIPLYEERFMLYGTRAAMRRKP
CCEEEEECCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
ETMTFAEVAELPLAAPDGAHDLRKIIERAANAIGRSLDVRYELNSPQMLIALARDGLAYV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEEEE
VMPPSACLEAVAAKSIVGRPVTEPDLTRIQALVWPGDRPLSPAAAAIRDLIAHIVGDLVE
EECHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RGALPGRTVGATYKKK
HCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNITLRQLRYFVEIAQSRSFSRAAERLRVAQPALSQNISSLEDSLGAKLFERHARGVEIS
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PAGERLLTSAIDILARTDALKDDIDGRASLPTGSVRLSIAGSIAGAVIAPLLGAVAREYP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCC
GIEVTVRESMSFESRALVESGQAHLALMPSPSEIQGMASIPLYEERFMLYGTRAAMRRKP
CCEEEEECCCCHHHHHHHHCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
ETMTFAEVAELPLAAPDGAHDLRKIIERAANAIGRSLDVRYELNSPQMLIALARDGLAYV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECCCEEEE
VMPPSACLEAVAAKSIVGRPVTEPDLTRIQALVWPGDRPLSPAAAAIRDLIAHIVGDLVE
EECHHHHHHHHHHHHHHCCCCCCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RGALPGRTVGATYKKK
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9524276; 12910271 [H]