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The map label for this gene is tmoF [H]

Identifier: 73539496

GI number: 73539496

Start: 2507800

End: 2508798

Strand: Reverse

Name: tmoF [H]

Synonym: Reut_B5674

Alternate gene names: 73539496

Gene position: 2508798-2507800 (Counterclockwise)

Preceding gene: 73539497

Following gene: 73539495

Centisome position: 92.03

GC content: 67.37

Gene sequence:

>999_bases
ATGAAATACCAGATATCGATCGAAGGCGGCGCGGTGTTCACCGTCGCCGCGGAGGAAGACACGCTGCTGCGCGGCGCCCT
GCGCGCCGGCATGGCCCTGCCGCACGAGTGCAGCGTGGGCGGCTGCGGCGCGTGCCGCTTCGACCTTGTGGACGGGCTCA
TGGAATCCGTCTGGCCTGAGGCGCCCGGCCTGTCCGAGCGTGACCGCAAGCGTGGCAAGTACCTTGCGTGCCAGTCGCGG
CCGCTAAGCGACTGCACGATACGTGTGCGCTGCGACGAATCGTATCGCCCGGCGGTCCGGGCGCATCGCCGCGCAGCGGA
ACTCCTGGCACGCCGCGCGCTGACCCCTGACATGAGCGAATTCACGTTCCGGGTTCCAGGCGCGACCGAGTTCCGGCCCG
GCCAGTACGCGCTGCTCTACCCGCCCCGTGCACCGGGCGCCCGCGCCTATTCGATGGCCAACCTGCCCAACGAGGAAGGC
ATCTGGAAGTTCGTGATCCGCCGCGTGCCGGGCGGGGCTGGCAGCAACGCGCTGTTCGACCAGGTCGGAATCGGGGACAG
CGTCGTGCTCGACGGGCCGTACGGCCACGCCTACCTTCGTGAGGACAGTGCCCGCGACATTGTCTGCATCGCCGGTGGCT
CGGGCCTGGCGCCAATGCTGTCGGTCGCACGCGGAGCACTCGCCGGTAGCGGTTCGCGGCGCGTCCACTTCTTTTATGGC
GCCCGGGGGCAGGCTGACCTCGGTGCCCTCGACGCCCTGGAAAAACTTGCCGAAGACAAGCGGGTTACGCTGTCGGTGGC
ACTGTCCGCACCGGAGAGCACCTGGAAGGGGCCAACGGGGTTCGTGCACGAGGAAGTCGAGCGTAACCTGACAGCTTCCC
TCGGCAGCTACGACTTCTATTTTGCCGGGCCGCCACTCATGATCGAAGCCATGCAGGCGCTCCTGATGCACAAGCACCAG
GTACCGTTCGGGCAGATCCGCTTCGACCGCTTTGTCTAG

Upstream 100 bases:

>100_bases
CTGCGCGGCCGTGCGCGACTTCTGGGACAGCATCGGCCTGGCAGGTCTGTAACGCAGGACATGCCGGGCGATACGACTCA
ACAGCCACCATCGGAACCCT

Downstream 100 bases:

>100_bases
GCCGGCGACATCTGCCTTGGGACGCACAGCGCACAGCCGCGACACCGGATAGCCTGATCGCAAATGCCGCGTGCGATTAC
CAATCGCCATATCTGGAAGT

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NA

Alternate protein names: Ferredoxin; Ferredoxin--NAD(+) reductase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKYQISIEGGAVFTVAAEEDTLLRGALRAGMALPHECSVGGCGACRFDLVDGLMESVWPEAPGLSERDRKRGKYLACQSR
PLSDCTIRVRCDESYRPAVRAHRRAAELLARRALTPDMSEFTFRVPGATEFRPGQYALLYPPRAPGARAYSMANLPNEEG
IWKFVIRRVPGGAGSNALFDQVGIGDSVVLDGPYGHAYLREDSARDIVCIAGGSGLAPMLSVARGALAGSGSRRVHFFYG
ARGQADLGALDALEKLAEDKRVTLSVALSAPESTWKGPTGFVHEEVERNLTASLGSYDFYFAGPPLMIEAMQALLMHKHQ
VPFGQIRFDRFV

Sequences:

>Translated_332_residues
MKYQISIEGGAVFTVAAEEDTLLRGALRAGMALPHECSVGGCGACRFDLVDGLMESVWPEAPGLSERDRKRGKYLACQSR
PLSDCTIRVRCDESYRPAVRAHRRAAELLARRALTPDMSEFTFRVPGATEFRPGQYALLYPPRAPGARAYSMANLPNEEG
IWKFVIRRVPGGAGSNALFDQVGIGDSVVLDGPYGHAYLREDSARDIVCIAGGSGLAPMLSVARGALAGSGSRRVHFFYG
ARGQADLGALDALEKLAEDKRVTLSVALSAPESTWKGPTGFVHEEVERNLTASLGSYDFYFAGPPLMIEAMQALLMHKHQ
VPFGQIRFDRFV
>Mature_332_residues
MKYQISIEGGAVFTVAAEEDTLLRGALRAGMALPHECSVGGCGACRFDLVDGLMESVWPEAPGLSERDRKRGKYLACQSR
PLSDCTIRVRCDESYRPAVRAHRRAAELLARRALTPDMSEFTFRVPGATEFRPGQYALLYPPRAPGARAYSMANLPNEEG
IWKFVIRRVPGGAGSNALFDQVGIGDSVVLDGPYGHAYLREDSARDIVCIAGGSGLAPMLSVARGALAGSGSRRVHFFYG
ARGQADLGALDALEKLAEDKRVTLSVALSAPESTWKGPTGFVHEEVERNLTASLGSYDFYFAGPPLMIEAMQALLMHKHQ
VPFGQIRFDRFV

Specific function: Electron transfer component of toluene 4-monooxygenase complex [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=182, Percent_Identity=33.5164835164835, Blast_Score=85, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323552, Length=168, Percent_Identity=27.3809523809524, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.18.1.3 [H]

Molecular weight: Translated: 36086; Mature: 36086

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYQISIEGGAVFTVAAEEDTLLRGALRAGMALPHECSVGGCGACRFDLVDGLMESVWPE
CEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
APGLSERDRKRGKYLACQSRPLSDCTIRVRCDESYRPAVRAHRRAAELLARRALTPDMSE
CCCCCHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHH
FTFRVPGATEFRPGQYALLYPPRAPGARAYSMANLPNEEGIWKFVIRRVPGGAGSNALFD
EEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHH
QVGIGDSVVLDGPYGHAYLREDSARDIVCIAGGSGLAPMLSVARGALAGSGSRRVHFFYG
HCCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEC
ARGQADLGALDALEKLAEDKRVTLSVALSAPESTWKGPTGFVHEEVERNLTASLGSYDFY
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
FAGPPLMIEAMQALLMHKHQVPFGQIRFDRFV
ECCCHHHHHHHHHHHHHHCCCCCCCEEEECCC
>Mature Secondary Structure
MKYQISIEGGAVFTVAAEEDTLLRGALRAGMALPHECSVGGCGACRFDLVDGLMESVWPE
CEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
APGLSERDRKRGKYLACQSRPLSDCTIRVRCDESYRPAVRAHRRAAELLARRALTPDMSE
CCCCCHHHHHCCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHH
FTFRVPGATEFRPGQYALLYPPRAPGARAYSMANLPNEEGIWKFVIRRVPGGAGSNALFD
EEEECCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCCCHHH
QVGIGDSVVLDGPYGHAYLREDSARDIVCIAGGSGLAPMLSVARGALAGSGSRRVHFFYG
HCCCCCEEEEECCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEC
ARGQADLGALDALEKLAEDKRVTLSVALSAPESTWKGPTGFVHEEVERNLTASLGSYDFY
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEE
FAGPPLMIEAMQALLMHKHQVPFGQIRFDRFV
ECCCHHHHHHHHHHHHHHCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1429451 [H]