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The map label for this gene is emrB [H]

Identifier: 73539392

GI number: 73539392

Start: 2387890

End: 2389395

Strand: Reverse

Name: emrB [H]

Synonym: Reut_B5570

Alternate gene names: 73539392

Gene position: 2389395-2387890 (Counterclockwise)

Preceding gene: 73539394

Following gene: 73539391

Centisome position: 87.65

GC content: 66.27

Gene sequence:

>1506_bases
ATGCAGATCGCTAGCCATCCGGAGATGGGCCACTGGCGGCTGTCGCTGTTCGGGCTGACGCTGGGCCTCGTGACCGGCAT
CGACTTCTCCTCCACGCTGATGATGGGCGTAGCCAGCCAGCACATCCAGGGCGGCGTGGGCGCCGCGCCCGAAGATTACC
TGTATGCGGTCTCGGCCTACGCGGCCACCGCGGTGCTGATGAACATGGTGCTCGACCAGATCGCGCGCCGTACAACGTAC
AAGCGCTTCACCATCGCGTCGCTGCTGATCTTCATCGTCGGCTCGCTGCTGTGCGCCGAAGCCGCCAGCCCGGCCGGACT
GATCGGCGGCAAGGCTGTACAGGGGTTGGGCGCCGGCGGATTGTTCGCGGCCTCGCGGATCCTGGTGCAGCTGGTATCGA
CGCCGGCCGAGCGCGCGCCGCTGATGCTGCGCTTTGGCGGCGGTGCGTTCGCCATGCTGGCCATCACGCCATGGCTCACC
AGCATCTTCCTTGACGACATCGGCTGGCGCGCGGTGTTCCTGTTCCAGGCCGGGATCTCGCTGCCGGTGCTGCTGTCGGT
GATCGCCACCTATCCCACGCGCGCGCCGCGCGATCCGCATCCGCCTGTCTCAACGCTGGACTGGCCGGCTGCGATCGCCG
CGGCACTCGGTGCGCTGGTCTTCCTGCATACGCTGCAGGAAATGCGCTACACGCGTTTTTTCAGCTCGCCGCAGATGCCG
CTGGCCGCGTTGCTCGGCGTGGGCCTGTTTGCGTTCAGCGGCTGGCGCCTGTACCGGCACCCGGACCCATGGATCGACTT
CTCGCGGCTGGCCGGCCGCCAGTACCTGTACGGGCTGGGCTTCTATACGCTGTACTACCTGCTGTCGTCGGCGTGGGCAT
TCCTGTTGCCCACGCTCACGCAGAGCGGCCTGGGGCTGACCTTCCGTACCACGTGCATGCTGCTCACGACCAGCGGCACG
GTTTCCGCCATCGGCGCGGTGCTGATCACGCTGGGTATGGCGCTGGTCTTCCGCAAGCGCCGCGTGATCGCGGTCGGCTT
TCTCATCTATGCAGGCGCGGCCATGCTGTTGTCGTACCAGTTGATGCCCGGAGCGCCCGACTACGCACTCGTGCCGGTGG
TGCTGCTGGAAGGCCTGACGCCGGTCCTGCTGATGGTGCAGGTCGCTTCGATGACCTACCTGGACCTGCCGGTCGAGGAC
TTCTCCCACGCGTACCAGTTCAAGAACGTCTGCAAGCAGATCGCCTCGGCCTCCGGTACCGGTCTGGCGAGCGTCTTCAT
GCAGGACGGCCTGGCGGCGCACCGCACGCACCTGGTCGAGCACGTCACGCGCTTCACGCCGTCGCTGCAGATGCCCGATG
CACTGTCCGCGTCGAACCTGGCAAAGATCTCGCTGGAAATCGACCGACAGGCCACCCTGCTGGCCGGCATGGACATGCTG
CACGGCTTTGCCGCGCTGTGCGTGGTCATGGCCATTTTCGTGCTGCTGCAGAAGAGCTTCCGTTGA

Upstream 100 bases:

>100_bases
AACGTCTGTCCCCGCTGTTTCCAGCGTCCCGGTCGCGCACAGCGCCACCACTTCCCCAAGCCAGGCCACCATGGCTACCG
CAGCCCCCGCCCGCCCTCGC

Downstream 100 bases:

>100_bases
TCCGCGTAACCCGATAACGGTGCAGGAGACAGCATGGCGGCGGCGCCCCGAACGGCGGCGCCGCCAGCACGTGCGTCGCT
GCGCACTGGCGAAACCGGCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MQIASHPEMGHWRLSLFGLTLGLVTGIDFSSTLMMGVASQHIQGGVGAAPEDYLYAVSAYAATAVLMNMVLDQIARRTTY
KRFTIASLLIFIVGSLLCAEAASPAGLIGGKAVQGLGAGGLFAASRILVQLVSTPAERAPLMLRFGGGAFAMLAITPWLT
SIFLDDIGWRAVFLFQAGISLPVLLSVIATYPTRAPRDPHPPVSTLDWPAAIAAALGALVFLHTLQEMRYTRFFSSPQMP
LAALLGVGLFAFSGWRLYRHPDPWIDFSRLAGRQYLYGLGFYTLYYLLSSAWAFLLPTLTQSGLGLTFRTTCMLLTTSGT
VSAIGAVLITLGMALVFRKRRVIAVGFLIYAGAAMLLSYQLMPGAPDYALVPVVLLEGLTPVLLMVQVASMTYLDLPVED
FSHAYQFKNVCKQIASASGTGLASVFMQDGLAAHRTHLVEHVTRFTPSLQMPDALSASNLAKISLEIDRQATLLAGMDML
HGFAALCVVMAIFVLLQKSFR

Sequences:

>Translated_501_residues
MQIASHPEMGHWRLSLFGLTLGLVTGIDFSSTLMMGVASQHIQGGVGAAPEDYLYAVSAYAATAVLMNMVLDQIARRTTY
KRFTIASLLIFIVGSLLCAEAASPAGLIGGKAVQGLGAGGLFAASRILVQLVSTPAERAPLMLRFGGGAFAMLAITPWLT
SIFLDDIGWRAVFLFQAGISLPVLLSVIATYPTRAPRDPHPPVSTLDWPAAIAAALGALVFLHTLQEMRYTRFFSSPQMP
LAALLGVGLFAFSGWRLYRHPDPWIDFSRLAGRQYLYGLGFYTLYYLLSSAWAFLLPTLTQSGLGLTFRTTCMLLTTSGT
VSAIGAVLITLGMALVFRKRRVIAVGFLIYAGAAMLLSYQLMPGAPDYALVPVVLLEGLTPVLLMVQVASMTYLDLPVED
FSHAYQFKNVCKQIASASGTGLASVFMQDGLAAHRTHLVEHVTRFTPSLQMPDALSASNLAKISLEIDRQATLLAGMDML
HGFAALCVVMAIFVLLQKSFR
>Mature_501_residues
MQIASHPEMGHWRLSLFGLTLGLVTGIDFSSTLMMGVASQHIQGGVGAAPEDYLYAVSAYAATAVLMNMVLDQIARRTTY
KRFTIASLLIFIVGSLLCAEAASPAGLIGGKAVQGLGAGGLFAASRILVQLVSTPAERAPLMLRFGGGAFAMLAITPWLT
SIFLDDIGWRAVFLFQAGISLPVLLSVIATYPTRAPRDPHPPVSTLDWPAAIAAALGALVFLHTLQEMRYTRFFSSPQMP
LAALLGVGLFAFSGWRLYRHPDPWIDFSRLAGRQYLYGLGFYTLYYLLSSAWAFLLPTLTQSGLGLTFRTTCMLLTTSGT
VSAIGAVLITLGMALVFRKRRVIAVGFLIYAGAAMLLSYQLMPGAPDYALVPVVLLEGLTPVLLMVQVASMTYLDLPVED
FSHAYQFKNVCKQIASASGTGLASVFMQDGLAAHRTHLVEHVTRFTPSLQMPDALSASNLAKISLEIDRQATLLAGMDML
HGFAALCVVMAIFVLLQKSFR

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=445, Percent_Identity=22.0224719101124, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788710, Length=490, Percent_Identity=21.6326530612245, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53987; Mature: 53987

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIASHPEMGHWRLSLFGLTLGLVTGIDFSSTLMMGVASQHIQGGVGAAPEDYLYAVSAY
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AATAVLMNMVLDQIARRTTYKRFTIASLLIFIVGSLLCAEAASPAGLIGGKAVQGLGAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCH
LFAASRILVQLVSTPAERAPLMLRFGGGAFAMLAITPWLTSIFLDDIGWRAVFLFQAGIS
HHHHHHHHHHHHHCCHHHCCEEEEECCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LPVLLSVIATYPTRAPRDPHPPVSTLDWPAAIAAALGALVFLHTLQEMRYTRFFSSPQMP
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LAALLGVGLFAFSGWRLYRHPDPWIDFSRLAGRQYLYGLGFYTLYYLLSSAWAFLLPTLT
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGLGLTFRTTCMLLTTSGTVSAIGAVLITLGMALVFRKRRVIAVGFLIYAGAAMLLSYQ
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMPGAPDYALVPVVLLEGLTPVLLMVQVASMTYLDLPVEDFSHAYQFKNVCKQIASASGT
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
GLASVFMQDGLAAHRTHLVEHVTRFTPSLQMPDALSASNLAKISLEIDRQATLLAGMDML
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
HGFAALCVVMAIFVLLQKSFR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIASHPEMGHWRLSLFGLTLGLVTGIDFSSTLMMGVASQHIQGGVGAAPEDYLYAVSAY
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AATAVLMNMVLDQIARRTTYKRFTIASLLIFIVGSLLCAEAASPAGLIGGKAVQGLGAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCH
LFAASRILVQLVSTPAERAPLMLRFGGGAFAMLAITPWLTSIFLDDIGWRAVFLFQAGIS
HHHHHHHHHHHHHCCHHHCCEEEEECCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LPVLLSVIATYPTRAPRDPHPPVSTLDWPAAIAAALGALVFLHTLQEMRYTRFFSSPQMP
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
LAALLGVGLFAFSGWRLYRHPDPWIDFSRLAGRQYLYGLGFYTLYYLLSSAWAFLLPTLT
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGLGLTFRTTCMLLTTSGTVSAIGAVLITLGMALVFRKRRVIAVGFLIYAGAAMLLSYQ
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMPGAPDYALVPVVLLEGLTPVLLMVQVASMTYLDLPVEDFSHAYQFKNVCKQIASASGT
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
GLASVFMQDGLAAHRTHLVEHVTRFTPSLQMPDALSASNLAKISLEIDRQATLLAGMDML
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
HGFAALCVVMAIFVLLQKSFR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]