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The map label for this gene is pcaR [H]

Identifier: 73539391

GI number: 73539391

Start: 2386897

End: 2387856

Strand: Reverse

Name: pcaR [H]

Synonym: Reut_B5569

Alternate gene names: 73539391

Gene position: 2387856-2386897 (Counterclockwise)

Preceding gene: 73539392

Following gene: 73539389

Centisome position: 87.59

GC content: 67.6

Gene sequence:

>960_bases
ATGGCGGCGGCGCCCCGAACGGCGGCGCCGCCAGCACGTGCGTCGCTGCGCACTGGCGAAACCGGCGTATTTCCTCTACG
CTTTCGGCCTTTGCGAGCCAATCCGGCGACCCGCGCCGGTGCCCGACAGATGGCCAGTCCCGCCGATCCCTCACAAGCCG
CGGAAACCGCCGCCACCTCCGCCGCCACGCCCGAAAAGCCCAGCGACAGCTATGTGCAGTCTTTTGCACGCGGCCTGGCC
GTGATTCGCGCGTTCAACGCCGAGCACCCGGCCCAGACCCTGACGGAAATCGCCCAGGCCAGCGGCCTCACGCGCGCCGG
CGCGCGCCGCATCCTGCTGACGCTGGTGGGCCTTGGCTATGTGCAGGCCGAAGGCAGGCTGTTCCGCCTGACCCCGAAGA
TCCTCGATCTTGGCTTTGCCTACCTGACCTCGATGCCGTTCTGGAACCTGGCCGAGCCGATCATGGAGGCGCTCTCGCAA
CAGGTCCACGAAAGCTGCTCGATCTCAGTGCTCGACGGCACCGAGATCGTCTACGTGCTGCGCGTGCCCGCCCGCAAGAT
CATGACCATCAACCTGTCGATCGGCAGCCGCTTGCCGGCCTATTGCTCCTCGATGGGGCGCGTGCTGCTGGCCGGCCTGC
CCCCCGATGAACTGGACCGCGTGCTGCGCAACACCGAACTGCGCGCGCGCACCAGCCGCACCGTGACCGATATCGATGCG
CTGAAGACCGTCATCGCCGATATCCGTACGCGCGGCTGGGCGCTGAATGACCAGGAACTGGAAGAAGGGTTGGTTTCGCT
GGCCGCGCCGATCCGCAACCGCGCGGGCCATACGATTGCCGCCCTCAACATCAGCGGCCAGGCCAACCGCACCAGCGCCG
CGGAAATGCGCGAGCGCTTTCTGCCTTCGCTGCTGGAAGCGGCCGACAAGATCTCCGGGCTGGTCGGCCTGCGCACCTGA

Upstream 100 bases:

>100_bases
GCACGGCTTTGCCGCGCTGTGCGTGGTCATGGCCATTTTCGTGCTGCTGCAGAAGAGCTTCCGTTGATCCGCGTAACCCG
ATAACGGTGCAGGAGACAGC

Downstream 100 bases:

>100_bases
GCGCCACAGGCCCCGCCCCGGGCACGCTTAAAGCTCGACGCTGCCGAATTCGGCCGAACGCGTGCCGTCCACGGGACGAT
TGCTGGCGCCGAAGTAGGCG

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAAAPRTAAPPARASLRTGETGVFPLRFRPLRANPATRAGARQMASPADPSQAAETAATSAATPEKPSDSYVQSFARGLA
VIRAFNAEHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQAEGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQ
QVHESCSISVLDGTEIVYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLAGLPPDELDRVLRNTELRARTSRTVTDIDA
LKTVIADIRTRGWALNDQELEEGLVSLAAPIRNRAGHTIAALNISGQANRTSAAEMRERFLPSLLEAADKISGLVGLRT

Sequences:

>Translated_319_residues
MAAAPRTAAPPARASLRTGETGVFPLRFRPLRANPATRAGARQMASPADPSQAAETAATSAATPEKPSDSYVQSFARGLA
VIRAFNAEHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQAEGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQ
QVHESCSISVLDGTEIVYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLAGLPPDELDRVLRNTELRARTSRTVTDIDA
LKTVIADIRTRGWALNDQELEEGLVSLAAPIRNRAGHTIAALNISGQANRTSAAEMRERFLPSLLEAADKISGLVGLRT
>Mature_318_residues
AAAPRTAAPPARASLRTGETGVFPLRFRPLRANPATRAGARQMASPADPSQAAETAATSAATPEKPSDSYVQSFARGLAV
IRAFNAEHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQAEGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQQ
VHESCSISVLDGTEIVYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLAGLPPDELDRVLRNTELRARTSRTVTDIDAL
KTVIADIRTRGWALNDQELEEGLVSLAAPIRNRAGHTIAALNISGQANRTSAAEMRERFLPSLLEAADKISGLVGLRT

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1788131, Length=257, Percent_Identity=32.295719844358, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786468, Length=227, Percent_Identity=29.0748898678414, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082359, Length=250, Percent_Identity=28.8, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789999, Length=263, Percent_Identity=28.1368821292776, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786715, Length=251, Percent_Identity=24.3027888446215, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790752, Length=211, Percent_Identity=24.6445497630332, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788581, Length=195, Percent_Identity=25.1282051282051, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 34263; Mature: 34132

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAPRTAAPPARASLRTGETGVFPLRFRPLRANPATRAGARQMASPADPSQAAETAATS
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHC
AATPEKPSDSYVQSFARGLAVIRAFNAEHPAQTLTEIAQASGLTRAGARRILLTLVGLGY
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCE
VQAEGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQQVHESCSISVLDGTEIVYVL
EECCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEE
RVPARKIMTINLSIGSRLPAYCSSMGRVLLAGLPPDELDRVLRNTELRARTSRTVTDIDA
ECCCCEEEEEEECCCCCCHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHHHCCCHHHHHH
LKTVIADIRTRGWALNDQELEEGLVSLAAPIRNRAGHTIAALNISGQANRTSAAEMRERF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
LPSLLEAADKISGLVGLRT
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAAPRTAAPPARASLRTGETGVFPLRFRPLRANPATRAGARQMASPADPSQAAETAATS
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHC
AATPEKPSDSYVQSFARGLAVIRAFNAEHPAQTLTEIAQASGLTRAGARRILLTLVGLGY
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCE
VQAEGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQQVHESCSISVLDGTEIVYVL
EECCCEEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEE
RVPARKIMTINLSIGSRLPAYCSSMGRVLLAGLPPDELDRVLRNTELRARTSRTVTDIDA
ECCCCEEEEEEECCCCCCHHHHHHHCCEEECCCCHHHHHHHHHCCHHHHHHCCCHHHHHH
LKTVIADIRTRGWALNDQELEEGLVSLAAPIRNRAGHTIAALNISGQANRTSAAEMRERF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHH
LPSLLEAADKISGLVGLRT
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]