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The map label for this gene is narK [H]

Identifier: 73538826

GI number: 73538826

Start: 1732129

End: 1733514

Strand: Direct

Name: narK [H]

Synonym: Reut_B5001

Alternate gene names: 73538826

Gene position: 1732129-1733514 (Clockwise)

Preceding gene: 73538825

Following gene: 73538827

Centisome position: 63.54

GC content: 65.01

Gene sequence:

>1386_bases
ATGTCCACTTCCGTACTGACCCGCTGGGAGCCCGAGAACAAGGCCTTCTGGCAGAACACCGGCGAGCGCATCGCCTACCG
CAACCTGTGGATTTCGATCCCCGCGCTGATGCTGGCCTTCGTGGTCTGGATGCTGTGGAGCGTGGTCGCGGTCAATCTTG
ATCGCGCTGGCTTCCAGTTCACCAAGAACCAACTGTTCTGGCTCACCGCACTGCCCGCTTTGTCCGGCGCCACGCTGCGC
ATCTTCTACTCGTTCCTCGTCCCGGTGTTCGGCGGTCGACGCTTCACCGCGATCTCCACGGCGCTGCTGCTGATTCCTGC
GCTCGGCATGGGCTTCGCGCTGCGCGATCCGTCCACGGGCTACCCGACGCTGCTTGCGCTCGCCTTGCTGTGCGGCTTCG
GCGGCGCCAACTTCAGTTCGTCGATGGCCAATATCAGCTTCTTCTTCCCGAAGGCGAAGAAGGGCCTGGCTACAGGCCTC
AATGCGGGCATCGGCAATCTCGGCGTGTCGGTCGTTCAGTTCGTCACGCCGCTCGTGGTATCGGTGGCCGTGTTCGGCAC
GCTTGGCGGCGAGCCCCAGCAGTACCAGGCCGGCGACGCCATGAAGAACCTGTGGCTGCAGAACGCGGGGTTCATCTGGG
TGCCGTTCATCGTGGTGTCGTCGCTTGCCGCATGGTTCGGCATGCATGACATCGCCGACGCCAAGGCGTCCTTCGCTGAC
CAGGCCATCATCTTCAAGCGCAAGCACAACTGGCTGATGTGCTGGCTCTACGTGGGCACGTTCGGCTCGTTCATCGGCTT
CTCGGCCGGCCTGGCGCTGCTGACCAAGTCGCAGTTTCCGGGCGTGAACCCGACCGCGTATGCGTTCCTCGGGCCGCTGG
TCGGCGCACTGACGCGTCCCGTGGGCGGCTGGATCTCCGACAAGCTTGGCGGCGCGCGCGTCACGCTGTGGACGTTTGTC
GGCATGATCGCCGCCGTGTTCGCCGTGCTGGCCGCACTGCCGCATGACGGCGCAGCGGGCAGTTTCCCGGCATTCCTCGC
AGCCTTCGTCGCGCTGTTCGCGCTGACTGGTGTCGGCAACGGCTCCACGTTCCGCATGATTCCGGTGATCTTCCTGACCG
AACGCCAGCGTGCCGCCAAGGGCAAGGGCGACACGGCGCAGAAGCAGGCATTGCAGGAAGCGGGCAAGGAATCCGCCGCG
GTGCTCGGCTTCTCCGGCGCGATCGGTGCCTACGGCGGCTTCTTCATCCCCAAGAGCTTTGGCACGTCGCTGGAACTGAC
CGGCTCCGCCGACGCCGCGCTGTATTGCTTCATCGCGTTCTACGTGAGCTGCGTGCTGGTGACGTGGTGGTGCTACGCGC
GCCGCAACGCAGCCATGCCTTGCTGA

Upstream 100 bases:

>100_bases
ACAGCCAACGCGCCGAACGCCGGCGCAAGGAGCAAGCAGTCGGCCTGACTCAGACCGCCTGACGCCTTCCTGACATCCCT
CAACACTTAAGCACACTGTC

Downstream 100 bases:

>100_bases
TGTGCTGCCGGCCGGCCTAGTTCGACAGAACTAGGCCGGCGCCCGCCGACTAGCCCGAACGCACCGCGCGGCCTGTTCCA
CCAGCGAATTGGCGCGCACT

Product: nitrate transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSTSVLTRWEPENKAFWQNTGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLALALLCGFGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAVFGTLGGEPQQYQAGDAMKNLWLQNAGFIWVPFIVVSSLAAWFGMHDIADAKASFAD
QAIIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLWTFV
GMIAAVFAVLAALPHDGAAGSFPAFLAAFVALFALTGVGNGSTFRMIPVIFLTERQRAAKGKGDTAQKQALQEAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLELTGSADAALYCFIAFYVSCVLVTWWCYARRNAAMPC

Sequences:

>Translated_461_residues
MSTSVLTRWEPENKAFWQNTGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLR
IFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLALALLCGFGGANFSSSMANISFFFPKAKKGLATGL
NAGIGNLGVSVVQFVTPLVVSVAVFGTLGGEPQQYQAGDAMKNLWLQNAGFIWVPFIVVSSLAAWFGMHDIADAKASFAD
QAIIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLWTFV
GMIAAVFAVLAALPHDGAAGSFPAFLAAFVALFALTGVGNGSTFRMIPVIFLTERQRAAKGKGDTAQKQALQEAGKESAA
VLGFSGAIGAYGGFFIPKSFGTSLELTGSADAALYCFIAFYVSCVLVTWWCYARRNAAMPC
>Mature_460_residues
STSVLTRWEPENKAFWQNTGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQFTKNQLFWLTALPALSGATLRI
FYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTGYPTLLALALLCGFGGANFSSSMANISFFFPKAKKGLATGLN
AGIGNLGVSVVQFVTPLVVSVAVFGTLGGEPQQYQAGDAMKNLWLQNAGFIWVPFIVVSSLAAWFGMHDIADAKASFADQ
AIIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRPVGGWISDKLGGARVTLWTFVG
MIAAVFAVLAALPHDGAAGSFPAFLAAFVALFALTGVGNGSTFRMIPVIFLTERQRAAKGKGDTAQKQALQEAGKESAAV
LGFSGAIGAYGGFFIPKSFGTSLELTGSADAALYCFIAFYVSCVLVTWWCYARRNAAMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=457, Percent_Identity=56.0175054704595, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=55.2401746724891, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49333; Mature: 49202

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSVLTRWEPENKAFWQNTGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCCEECCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLALALLCGFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVAVFGTLGGEPQQYQAGDAMKNLWLQNAGFIWVPFIVVSSLAAWFGMHDIADAKASFAD
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAIIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VGGWISDKLGGARVTLWTFVGMIAAVFAVLAALPHDGAAGSFPAFLAAFVALFALTGVGN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAKGKGDTAQKQALQEAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECCCCC
GTSLELTGSADAALYCFIAFYVSCVLVTWWCYARRNAAMPC
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STSVLTRWEPENKAFWQNTGERIAYRNLWISIPALMLAFVVWMLWSVVAVNLDRAGFQF
CCCCEECCCCCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
TKNQLFWLTALPALSGATLRIFYSFLVPVFGGRRFTAISTALLLIPALGMGFALRDPSTG
CCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCC
YPTLLALALLCGFGGANFSSSMANISFFFPKAKKGLATGLNAGIGNLGVSVVQFVTPLVV
CHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SVAVFGTLGGEPQQYQAGDAMKNLWLQNAGFIWVPFIVVSSLAAWFGMHDIADAKASFAD
HHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QAIIFKRKHNWLMCWLYVGTFGSFIGFSAGLALLTKSQFPGVNPTAYAFLGPLVGALTRP
HHEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
VGGWISDKLGGARVTLWTFVGMIAAVFAVLAALPHDGAAGSFPAFLAAFVALFALTGVGN
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
GSTFRMIPVIFLTERQRAAKGKGDTAQKQALQEAGKESAAVLGFSGAIGAYGGFFIPKSF
CCCEEEEEEEEEECHHHHHCCCCCHHHHHHHHHCCCCCEEEEECCCCHHCCCCEECCCCC
GTSLELTGSADAALYCFIAFYVSCVLVTWWCYARRNAAMPC
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]