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The map label for this gene is narK [H]

Identifier: 73538825

GI number: 73538825

Start: 1730801

End: 1732090

Strand: Direct

Name: narK [H]

Synonym: Reut_B5000

Alternate gene names: 73538825

Gene position: 1730801-1732090 (Clockwise)

Preceding gene: 73538823

Following gene: 73538826

Centisome position: 63.49

GC content: 65.97

Gene sequence:

>1290_bases
ATGGCCACCCCCACACGCGCGGCGCGCATGCCGGAGCCCGTCCCTTCCGGGGCATGGACGACGCTGGCATCCAGCACGTT
CGCCTTCACCATCTGCTTTGCCGTCTGGATGCTATTTGCCGTCCTCGGCATCCCGCTCAAGAAGGAACTCGGGCTCAATG
AAACCGAGTTCGGCCTGCTCGCCGCTACGCCCGTGCTGAGCGGCTCGCTGATCCGCGTGCCGCTAGGCCTGTGGACCGAC
CGCTACGGCGGCCGTGTCGTGTTCTTCGTGCTGATGCTGGCTACCGTCATCCCGATCTGGCTGATCTCGTACGCGCACAC
GCTGTGGCAACTGCTGGTGCTCGGCCTGTTCGTCGGTCTGGCGGGCGGTTCGTTTTCCGTCGGCACGCCTTACGTGGCAC
GCTGGTTCCCGCGTTCTCGCCAAGGGCTCGCGATGGGGATCTTCGGTGCCGGTAACTCCGGTGCCGCGCTGACCAAGTTT
GTCGCACCCGCGCTGATCCTCGCCGCTGGCACCTGGGAAATCGTGCCGCGCGTGTATTCGGTGGCGATGCTGGTGACGGC
CATCGTGTTCTGGCTCGGGTCGCGCAGCAATCCCGCCCACCGGGTGATGACGAGTGCCGGCTGGCGCGAGCAGCTTGCCG
TGATGCGCGACCCGCGCGTGTGGCGCTACTCGCAGTACTACTCGGTGGTGTTCGGCGGCTATGTCGGCCTGTCGCTGTGG
ATGACCAAGTACTACATCGGCGAATACGGCTTCGATATCAAGGTGGCCGCCTTCCTGGCCGCCTGCTTCTCGCTGCCCGG
CGGCGTGCTGCGCGCGATCGGCGGCTGGATCTCCGACCGCTACGGCGCGCACCGCACCACGTGGTGGGTCATGTGGGTGA
GCTGGGTCGGCTTCTTCCTGCTGAGCTACCCGCAAACCGACTTCGTGATCCACACCACCACGGGCCCGCAGAGCTTCCGC
ATCGCGCTGACGCCAGCGGTGTTCACCTTCCTGCTGTTCGTGGTCGGCATTGCGTTCGCCATCGGCAAGGCCTCGGTCTT
CAAGTTCATCTCGAACGATTTCACGCACAACATCGGCGCCGTCTCCGGCGTAGTCGGGCTCGCAGGCGGGCTGGGCGGCT
TCGTGCTGCCGATCCTGTTCGGCGCGCTGGCCGACCTCACCGGCGTGCGCAGCAGCTGCTTCATGCTGATGTACGGCACG
GTGTGCGTAAGCCTGGTCTGGATGCACTACAGCCAACGCGCCGAACGCCGGCGCAAGGAGCAAGCAGTCGGCCTGACTCA
GACCGCCTGA

Upstream 100 bases:

>100_bases
AGGTTCCGCGGCGTGGCGGCTTGTGCTCCCGGCGAATGGTCAGCCGCAGCGGTCGTGCCTACGCTGGCATCACCGTCAAA
GTTGACACAGGAGCATGGCC

Downstream 100 bases:

>100_bases
CGCCTTCCTGACATCCCTCAACACTTAAGCACACTGTCATGTCCACTTCCGTACTGACCCGCTGGGAGCCCGAGAACAAG
GCCTTCTGGCAGAACACCGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MATPTRAARMPEPVPSGAWTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNETEFGLLAATPVLSGSLIRVPLGLWTD
RYGGRVVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKF
VAPALILAAGTWEIVPRVYSVAMLVTAIVFWLGSRSNPAHRVMTSAGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
MTKYYIGEYGFDIKVAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFVIHTTTGPQSFR
IALTPAVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPILFGALADLTGVRSSCFMLMYGT
VCVSLVWMHYSQRAERRRKEQAVGLTQTA

Sequences:

>Translated_429_residues
MATPTRAARMPEPVPSGAWTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNETEFGLLAATPVLSGSLIRVPLGLWTD
RYGGRVVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKF
VAPALILAAGTWEIVPRVYSVAMLVTAIVFWLGSRSNPAHRVMTSAGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
MTKYYIGEYGFDIKVAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFVIHTTTGPQSFR
IALTPAVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPILFGALADLTGVRSSCFMLMYGT
VCVSLVWMHYSQRAERRRKEQAVGLTQTA
>Mature_428_residues
ATPTRAARMPEPVPSGAWTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNETEFGLLAATPVLSGSLIRVPLGLWTDR
YGGRVVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGLAGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFV
APALILAAGTWEIVPRVYSVAMLVTAIVFWLGSRSNPAHRVMTSAGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLWM
TKYYIGEYGFDIKVAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFLLSYPQTDFVIHTTTGPQSFRI
ALTPAVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGAVSGVVGLAGGLGGFVLPILFGALADLTGVRSSCFMLMYGTV
CVSLVWMHYSQRAERRRKEQAVGLTQTA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=446, Percent_Identity=27.5784753363229, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1787475, Length=446, Percent_Identity=25.1121076233184, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46884; Mature: 46752

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPTRAARMPEPVPSGAWTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNETEFGLL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCE
AATPVLSGSLIRVPLGLWTDRYGGRVVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGL
EECHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPALILAAGTWEIVPRVYS
CCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VAMLVTAIVFWLGSRSNPAHRVMTSAGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
HHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
MTKYYIGEYGFDIKVAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFL
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSYPQTDFVIHTTTGPQSFRIALTPAVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGA
HCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGVVGLAGGLGGFVLPILFGALADLTGVRSSCFMLMYGTVCVSLVWMHYSQRAERRRKE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVGLTQTA
HHCCCCCCC
>Mature Secondary Structure 
ATPTRAARMPEPVPSGAWTTLASSTFAFTICFAVWMLFAVLGIPLKKELGLNETEFGLL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCE
AATPVLSGSLIRVPLGLWTDRYGGRVVFFVLMLATVIPIWLISYAHTLWQLLVLGLFVGL
EECHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGSFSVGTPYVARWFPRSRQGLAMGIFGAGNSGAALTKFVAPALILAAGTWEIVPRVYS
CCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
VAMLVTAIVFWLGSRSNPAHRVMTSAGWREQLAVMRDPRVWRYSQYYSVVFGGYVGLSLW
HHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHH
MTKYYIGEYGFDIKVAAFLAACFSLPGGVLRAIGGWISDRYGAHRTTWWVMWVSWVGFFL
HHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LSYPQTDFVIHTTTGPQSFRIALTPAVFTFLLFVVGIAFAIGKASVFKFISNDFTHNIGA
HCCCCCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSGVVGLAGGLGGFVLPILFGALADLTGVRSSCFMLMYGTVCVSLVWMHYSQRAERRRKE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVGLTQTA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]