Click here to switch to the map view.

The map label for this gene is flhA [H]

Identifier: 73538658

GI number: 73538658

Start: 1538694

End: 1539800

Strand: Direct

Name: flhA [H]

Synonym: Reut_B4833

Alternate gene names: 73538658

Gene position: 1538694-1539800 (Clockwise)

Preceding gene: 73538657

Following gene: 73538659

Centisome position: 56.44

GC content: 64.86

Gene sequence:

>1107_bases
ATGAAAACACGCGCAGCAGTTGCCTGGGGGGCGGGAAAGCCGCTAACGATCGAAGACGTGGACCTGGAAGGCCCCCGCGC
CGGTGAAGTGCTGGTAGAAATCAAGGCCACCGGCATCTGCCATACCGATTACTACACGCTGTCCGGCGCCGATCCGGAAG
GCATCTTCCCGGCGATCCTCGGGCACGAGGGCGCGGGCATCGTCACCGATGTCGGTGCCGGCGTGACCTCGCTGAAGCCC
GGCGACCACGTGATTCCGCTCTACACGCCGGAATGCCGCCAATGCAAGTTCTGCCTGTCGCGCAAGACCAACCTGTGCCA
GGCCATCCGCGCCACGCAGGGCAAGGGCCTGATGCCGGACGGCACCTCGCGCTTTTCGATCGATGGCCAGCCGATCTTCC
ACTACATGGGCACGTCGACGTTCGCCAACCACATCGTGGTCCCGGAAATCGCGCTCGCGAAGATTCGCCCCGACGCGCCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTGACCACCGGCGTGGGCGCCGTCATCTTCACCGCGAAGGTGGAAGCCGG
CGCCAACGTGGTGGTGTTCGGCCTCGGCGGCATCGGCCTGAACGTGATCCAGGGCGCGAAGATGGTCGGCGCGGACAAGA
TCATCGGCGTGGACCTGAACCCCGAGCGCGAAGCGATGGCGCGCAAGTTCGGCATGACGCATTTCATCAACCCGAAGGAC
GTGGGCAACGTCGTCGACCACATCATCCAGCTCACCGACGGCGGCGCGGACTACTCGTTCGAATGCATCGGCAATACGCA
GGTCATGCGCCAGGCGCTCGAGTGCTGCCACAAGGGCTGGGGCAAGTCGATCATCATCGGCGTGGCGGAGGCCGGCGCGG
AGATCTCCACGCGTCCGTTCCAGCTCGTCACGGGTCGCGAATGGAAGGGCTCGGCTTTCGGCGGCGCGCGCGGACGCACT
GACGTGCCCAAGATCGTGGACTGGTACATGGACGGCAAGCTCAACATCGACGACCTGATCACGCACACGCTGCCGCTCGA
CCGCATCAACGAAGGCTTCGACCTGATGAAGCGCGGCGAGTCGATCCGCTCGGTGGTGCTGTACTGA

Upstream 100 bases:

>100_bases
TTGAGAGCGAGTGTTGAACCTGTACCTACGCCCACGGACCATCACCTCGTCGGGCCACCAATCATTTGACGCCAACAGCG
TATTTCCACAGAAGAGAGCC

Downstream 100 bases:

>100_bases
GGAGCGCCTTTCCCGAGAACACCCTTGTAGGCAGGGAGCGGATGACCGATTCCTGCCGACTCCGCCGCGACCCATCGATG
TATTGAATTAGGCACTCCCG

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTRAAVAWGAGKPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGAGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGQPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY

Sequences:

>Translated_368_residues
MKTRAAVAWGAGKPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGAGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGQPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY
>Mature_368_residues
MKTRAAVAWGAGKPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGAGVTSLKP
GDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGQPIFHYMGTSTFANHIVVPEIALAKIRPDAP
FDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.5803814713896, Blast_Score=464, Evalue=1e-131,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.3262032085562, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.6124661246612, Blast_Score=359, Evalue=2e-99,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.5284552845528, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.1351351351351, Blast_Score=342, Evalue=2e-94,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.1351351351351, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=44.5652173913043, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.5983379501385, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI156627571, Length=361, Percent_Identity=25.7617728531856, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=59.8915989159892, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=26.4397905759162, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790045, Length=363, Percent_Identity=28.099173553719, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=26.123595505618, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786825, Length=400, Percent_Identity=25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081918, Length=344, Percent_Identity=28.1976744186047, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI226510992, Length=374, Percent_Identity=24.5989304812834, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=25.0681198910082, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.2278820375335, Blast_Score=436, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.9333333333333, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=55.3935860058309, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17562584, Length=359, Percent_Identity=27.2980501392758, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=369, Percent_Identity=28.1842818428184, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=365, Percent_Identity=25.2054794520548, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562878, Length=368, Percent_Identity=26.3586956521739, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71988145, Length=369, Percent_Identity=26.0162601626016, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=54.9865229110512, Blast_Score=438, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=28.9544235924933, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=27.027027027027, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=369, Percent_Identity=27.6422764227642, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=363, Percent_Identity=26.9972451790634, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=365, Percent_Identity=28.2191780821918, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=365, Percent_Identity=25.2054794520548, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=365, Percent_Identity=28.2191780821918, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=61.9565217391304, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17137530, Length=358, Percent_Identity=27.9329608938547, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39364; Mature: 39364

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAWGAGKPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCEEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGAGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGQPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
HHHHEECC
>Mature Secondary Structure
MKTRAAVAWGAGKPLTIEDVDLEGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEECCCCCEEEEECCCCCCCCCCEEEEEEECEEEECCEEEECCCCCCCCCHHHH
GHEGAGIVTDVGAGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPD
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
GTSRFSIDGQPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIF
CCCEEEECCCEEEEECCCHHHHCCEECCCEEEEEECCCCCCCCEEEEECCCHHCCCEEEE
TAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPEREAMARKFGMTHFINPKD
EEEECCCCCEEEEECCCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCCCCCCHHH
VGNVVDHIIQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPF
HHHHHHHHHEECCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHCCCHHHHCCCHHHHHCCC
SIRSVVLY
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]