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The map label for this gene is thlA [H]

Identifier: 73538563

GI number: 73538563

Start: 1437273

End: 1438457

Strand: Reverse

Name: thlA [H]

Synonym: Reut_B4738

Alternate gene names: 73538563

Gene position: 1438457-1437273 (Counterclockwise)

Preceding gene: 73538564

Following gene: 73538562

Centisome position: 52.77

GC content: 66.92

Gene sequence:

>1185_bases
ATGAATACGCGCGAAGTGGTGTTGTGTCACCCGGTTCGCACCGCGATTGGTGCATTCAACGGCACCCTGAAGGGCACGCC
TGCGGTTGAACTCGGGGCCGCGGCAATCGGAGAGTCGCTCAAGCGTGCAGGCATCGATGCCGGCCGCATCGGCAGTGTGG
TGTTCGGCAACGTGATTCAGGCCGGCAACCGCATGAACCCTGCACGGCAGGCCGCCATCGGCGCGGGCTTGCCGGTATCC
GTCCCGGCCATGACCGTGAACCGCGTGTGCGGGTCCGGTGCGCAGGCCATTGCAACGGCCGCGGACGAGGTCAGGCTTGG
CTATGCCGACGTCGCCGTCGCCGGCGGCATGGAGAACATGGACCGTGCGCCTTACCTCGTGCCATCCGGCCGCTGGGGGC
AGCGCATGGGCGATACGGTCATGCATGACAGCATGCTGTTCGACGGGCTGCAGGATGCGTTCTCCGGCCTGCACTCGGGC
TGGCATACCGAGGATCTCGCCACCCGCTACGAGCTCAGCCGCGATGCACAGGACCGCTGGGCCGAGCGGTCGCAGCAGCG
CTTTGCGCGAGCGCAGGCGCAGGGCCGATTCGCGTCGCAGATCGTTGAAGTCGCCGTGAAGGCCAAGGGCGGTACGGCCG
CGTTCAAGGTCGACGAGGCCAATCGTCCCGATACCACCCTCGAGACGCTGGCGCGACTGAAGGCGGCATTTCGTGAGGGT
GGAACCATTACTGCCGGCAATGCGCCCGGCATCAACAGTGGTGCCGCTGCCATGCTGGTGGCCGAACGCGCCTTTGCGGA
AAGCCAGGGCATGAAGCCGATGGCTAGGCTGGTTGCCTACGGTGTCGGCGCGGTGGAACCGGGATTGTTCGGGCTTGGCC
CCGTTCCTGCCGTGCGCCAGGCGCTCGAGCGCGCACGCTGGACGCTCGGCGATATCGAGCGTATCGAGATCAACGAGGCC
TTCGCTGCCGTGCCGCTCGCAGTCGCGGCCGAGTTGGGCCTGCCCCACGACATCATCAACGTGGACGGCGGTGCCATTGC
GCACGGGCATCCGATCGGTGCGACCGGTGCGGTGCTGACGACGCGGCTGCTCCATGCGATGCAGTCGGACGGCATCCGGC
GCGGCATTGTCACGCTCTGCATTGGCGGCGGGCAGGGCATTGCGCTTGCCATCGAATCTCTCTGA

Upstream 100 bases:

>100_bases
AGTAAGTGGTAGCTGAACCGACGCTGCTGCTATGTCAGTGCCATCCGGTGCCGCTTGTGGCGCCCGCAACCGTCGTCGAC
GTCCGTAGGAGGGTAGTTTC

Downstream 100 bases:

>100_bases
ACAGACGAGACCTTATGAAGATACTTGTGCTGGGCGCGGGCGCCATGGGCGGTTACTATGGCGCGCGCCTGATCGAGGCC
GGCGCGGACGTCACGTTCCT

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNTREVVLCHPVRTAIGAFNGTLKGTPAVELGAAAIGESLKRAGIDAGRIGSVVFGNVIQAGNRMNPARQAAIGAGLPVS
VPAMTVNRVCGSGAQAIATAADEVRLGYADVAVAGGMENMDRAPYLVPSGRWGQRMGDTVMHDSMLFDGLQDAFSGLHSG
WHTEDLATRYELSRDAQDRWAERSQQRFARAQAQGRFASQIVEVAVKAKGGTAAFKVDEANRPDTTLETLARLKAAFREG
GTITAGNAPGINSGAAAMLVAERAFAESQGMKPMARLVAYGVGAVEPGLFGLGPVPAVRQALERARWTLGDIERIEINEA
FAAVPLAVAAELGLPHDIINVDGGAIAHGHPIGATGAVLTTRLLHAMQSDGIRRGIVTLCIGGGQGIALAIESL

Sequences:

>Translated_394_residues
MNTREVVLCHPVRTAIGAFNGTLKGTPAVELGAAAIGESLKRAGIDAGRIGSVVFGNVIQAGNRMNPARQAAIGAGLPVS
VPAMTVNRVCGSGAQAIATAADEVRLGYADVAVAGGMENMDRAPYLVPSGRWGQRMGDTVMHDSMLFDGLQDAFSGLHSG
WHTEDLATRYELSRDAQDRWAERSQQRFARAQAQGRFASQIVEVAVKAKGGTAAFKVDEANRPDTTLETLARLKAAFREG
GTITAGNAPGINSGAAAMLVAERAFAESQGMKPMARLVAYGVGAVEPGLFGLGPVPAVRQALERARWTLGDIERIEINEA
FAAVPLAVAAELGLPHDIINVDGGAIAHGHPIGATGAVLTTRLLHAMQSDGIRRGIVTLCIGGGQGIALAIESL
>Mature_394_residues
MNTREVVLCHPVRTAIGAFNGTLKGTPAVELGAAAIGESLKRAGIDAGRIGSVVFGNVIQAGNRMNPARQAAIGAGLPVS
VPAMTVNRVCGSGAQAIATAADEVRLGYADVAVAGGMENMDRAPYLVPSGRWGQRMGDTVMHDSMLFDGLQDAFSGLHSG
WHTEDLATRYELSRDAQDRWAERSQQRFARAQAQGRFASQIVEVAVKAKGGTAAFKVDEANRPDTTLETLARLKAAFREG
GTITAGNAPGINSGAAAMLVAERAFAESQGMKPMARLVAYGVGAVEPGLFGLGPVPAVRQALERARWTLGDIERIEINEA
FAAVPLAVAAELGLPHDIINVDGGAIAHGHPIGATGAVLTTRLLHAMQSDGIRRGIVTLCIGGGQGIALAIESL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=389, Percent_Identity=43.7017994858612, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI167614485, Length=382, Percent_Identity=38.7434554973822, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=36.4795918367347, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI4501853, Length=395, Percent_Identity=38.2278481012658, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI4504327, Length=430, Percent_Identity=32.093023255814, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194353979, Length=395, Percent_Identity=29.1139240506329, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=48.2142857142857, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=44.7300771208226, Blast_Score=323, Evalue=9e-90,
Organism=Escherichia coli, GI1787663, Length=398, Percent_Identity=43.7185929648241, Blast_Score=280, Evalue=8e-77,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=37.1859296482412, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788683, Length=403, Percent_Identity=31.2655086848635, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI133906874, Length=395, Percent_Identity=40.7594936708861, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=37.3096446700508, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=35.5163727959698, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535917, Length=399, Percent_Identity=30.0751879699248, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17551802, Length=427, Percent_Identity=30.4449648711944, Blast_Score=150, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=397, Percent_Identity=37.7833753148615, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6322031, Length=392, Percent_Identity=31.8877551020408, Blast_Score=180, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=45.6410256410256, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI17648125, Length=394, Percent_Identity=39.0862944162437, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24640423, Length=391, Percent_Identity=38.1074168797954, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=30.5164319248826, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40941; Mature: 40941

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTREVVLCHPVRTAIGAFNGTLKGTPAVELGAAAIGESLKRAGIDAGRIGSVVFGNVIQ
CCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGNRMNPARQAAIGAGLPVSVPAMTVNRVCGSGAQAIATAADEVRLGYADVAVAGGMENM
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCC
DRAPYLVPSGRWGQRMGDTVMHDSMLFDGLQDAFSGLHSGWHTEDLATRYELSRDAQDRW
CCCCEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
AERSQQRFARAQAQGRFASQIVEVAVKAKGGTAAFKVDEANRPDTTLETLARLKAAFREG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
GTITAGNAPGINSGAAAMLVAERAFAESQGMKPMARLVAYGVGAVEPGLFGLGPVPAVRQ
CEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALERARWTLGDIERIEINEAFAAVPLAVAAELGLPHDIINVDGGAIAHGHPIGATGAVLT
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHEEECCCCEEECCCCCCCHHHHHH
TRLLHAMQSDGIRRGIVTLCIGGGQGIALAIESL
HHHHHHHHHCCHHCCEEEEEEECCCEEEEEEECC
>Mature Secondary Structure
MNTREVVLCHPVRTAIGAFNGTLKGTPAVELGAAAIGESLKRAGIDAGRIGSVVFGNVIQ
CCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AGNRMNPARQAAIGAGLPVSVPAMTVNRVCGSGAQAIATAADEVRLGYADVAVAGGMENM
CCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCCCC
DRAPYLVPSGRWGQRMGDTVMHDSMLFDGLQDAFSGLHSGWHTEDLATRYELSRDAQDRW
CCCCEECCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHH
AERSQQRFARAQAQGRFASQIVEVAVKAKGGTAAFKVDEANRPDTTLETLARLKAAFREG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
GTITAGNAPGINSGAAAMLVAERAFAESQGMKPMARLVAYGVGAVEPGLFGLGPVPAVRQ
CEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHH
ALERARWTLGDIERIEINEAFAAVPLAVAAELGLPHDIINVDGGAIAHGHPIGATGAVLT
HHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHEEECCCCEEECCCCCCCHHHHHH
TRLLHAMQSDGIRRGIVTLCIGGGQGIALAIESL
HHHHHHHHHCCHHCCEEEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]