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The map label for this gene is gloB [H]

Identifier: 73538079

GI number: 73538079

Start: 905658

End: 907358

Strand: Direct

Name: gloB [H]

Synonym: Reut_B4249

Alternate gene names: 73538079

Gene position: 905658-907358 (Clockwise)

Preceding gene: 73538076

Following gene: 73538082

Centisome position: 33.22

GC content: 68.78

Gene sequence:

>1701_bases
ATGACAACGACAGAGACAAGCACTACCACGAACCGACCCGCCAGTCCCTCCAAGTCCCTGCGCGCCGCAGCCAGCCTGCT
GGTGGTGCGCGACGGCCCGCAGGGCATGGAAGTCCTGCTGGTGCGGCGCGTCGAGCGCGCCAACGACCGCAGCAGCGGCG
CCTTCGTGTTTCCGGGCGGCACGCTCGACCCGGAACAGGACAAGGCGCTGCACATTCATGCTGCCGGCCTCGACGACACC
GCCGCCAGCGAGCGCCTGAACCTGCCGGCCAACGGCCTGGACTTTTACCTGGCTGCTGTCCGCGAATGCTTCGAGGAAGC
GGGACTGCTGCTTGCCTACCACCGCAACGGCGATCTGCTGGCCCCGGGCCATCTCGACGACGCGCAACTCGATTTCCTGC
GCCAGGCGGTGCAGCGTGGCGGCCCCGGACTGGCGCACGCATGCGCCGAACTGGGCCTGCGCCTGGCATCCGACCGCCTG
GCATACCACAGCTACTGGCTCACGCCGCCGGGCCTGCCCAAGCGCTTCGACACGCGCTTCTTTGTCGCGATCGCGCCTGC
GGGCCAGATCGCCATCCACGATGGCATGGAAATCGTCGAGCATCGCTGGATCCGCCCCGGCGAAGCCGCGGACCCGGCCA
GCGGCCTGCCGATGATGCATGTCACGCGCCGCACGCTGCAGTCGATCGCGCATTTTGAAACGGCGCAGGAATGCTTTGCC
AGCACCTCGCTGCTGCGCGACATCGCGTGCATCATGCCCCGGCTGGCCAACGGTGCGGCGGGCGTGCGGCCCGTCATGCC
CAACGAGTCGTGCTACGCCGAGATCGGCCGGATTGACCCTGAAGGCAAGACGCACGCACGCTATGAACTGGCTCCGGGCG
AGCCGGTGCGGCTGTCCGAACGCGTCTGGCGCGTCACGGCCAACAACGGCAGCGTCATGACCGGGCCGGGCACCAATACA
TACCTGGTCGGTGGCGGTGCACGCAATGAATGGGCCGTGATCGATCCGGGCCCCGACGACGCCGCGCATGTGCAGGCAGT
GCTCGCTGCCGCGCCGGGCCCGATCCGCTGGATCCTGGCCACGCACACGCACATGGACCACTCGCCCGCCGCGCCCGCAT
TGCGGGCCGCCACAGGCGCCACCGTGCTTGGCCGCGCCGCACCGGCCACGCAGTGGCAGGACATCGCGTTTGCGCCGGCC
CGCGACCTGCAGCACGGCGAGCGCATCGCCATCGGCGACGACTGCACGCTGCGCGTGATCCATACGCCGGGTCATGCGTC
CAACCACCTGTGTTTCCTGCTCGAGGAAGAGAAAACGCTTTTCACCGGCGACCACGTCATGCAGGGCTCGACGGTCGTCA
TCTCCCCGCCCGACGGCGATATGCGCGCCTATCTGGATTCACTGGCCGCGTTGCAGGAAGAGGATCTCGAATGGCTGGCT
CCGGGCCACGGCTTCCTGATCACCCGTCCGCAGGAGGCCATCCGTCTGCTTGTGCGCCACCGCCTGCACCGCGAAGCCAA
GGTGCTCAGCGCGCTGCGCGATCTCGGGCCGGCCCCGATCGAGGCGCTGCTACCGCGCGTCTATGACGACGTGCCGGAGC
GCATGTACCCGGTCGCCCAGCGCTCGCTGCATGCGCACCTGCTCAAGCTTGTCGCCGATGGCAAGGCCGGGGACACAGAC
GGCCGCTGGCAAGCCGTCTAG

Upstream 100 bases:

>100_bases
AAACGCGGCCCGGAACGCTGCGCGAAACTTGGTACCACGCCGGCGCCAGACCGGTCCAGCCACACACTGCCGCGCGGCCC
TGCCCCCGCGCGCTGACACA

Downstream 100 bases:

>100_bases
CGCCGCTATGGCTGGGACGGGGCGGCGGGCGCGCCTGCCTGCCACGTCCGCCGCAGCGTACGCAGGTCGTCGGCCGACGC
GTCGGTAATGGCCAGGAAAC

Product: NUDIX hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MTTTETSTTTNRPASPSKSLRAAASLLVVRDGPQGMEVLLVRRVERANDRSSGAFVFPGGTLDPEQDKALHIHAAGLDDT
AASERLNLPANGLDFYLAAVRECFEEAGLLLAYHRNGDLLAPGHLDDAQLDFLRQAVQRGGPGLAHACAELGLRLASDRL
AYHSYWLTPPGLPKRFDTRFFVAIAPAGQIAIHDGMEIVEHRWIRPGEAADPASGLPMMHVTRRTLQSIAHFETAQECFA
STSLLRDIACIMPRLANGAAGVRPVMPNESCYAEIGRIDPEGKTHARYELAPGEPVRLSERVWRVTANNGSVMTGPGTNT
YLVGGGARNEWAVIDPGPDDAAHVQAVLAAAPGPIRWILATHTHMDHSPAAPALRAATGATVLGRAAPATQWQDIAFAPA
RDLQHGERIAIGDDCTLRVIHTPGHASNHLCFLLEEEKTLFTGDHVMQGSTVVISPPDGDMRAYLDSLAALQEEDLEWLA
PGHGFLITRPQEAIRLLVRHRLHREAKVLSALRDLGPAPIEALLPRVYDDVPERMYPVAQRSLHAHLLKLVADGKAGDTD
GRWQAV

Sequences:

>Translated_566_residues
MTTTETSTTTNRPASPSKSLRAAASLLVVRDGPQGMEVLLVRRVERANDRSSGAFVFPGGTLDPEQDKALHIHAAGLDDT
AASERLNLPANGLDFYLAAVRECFEEAGLLLAYHRNGDLLAPGHLDDAQLDFLRQAVQRGGPGLAHACAELGLRLASDRL
AYHSYWLTPPGLPKRFDTRFFVAIAPAGQIAIHDGMEIVEHRWIRPGEAADPASGLPMMHVTRRTLQSIAHFETAQECFA
STSLLRDIACIMPRLANGAAGVRPVMPNESCYAEIGRIDPEGKTHARYELAPGEPVRLSERVWRVTANNGSVMTGPGTNT
YLVGGGARNEWAVIDPGPDDAAHVQAVLAAAPGPIRWILATHTHMDHSPAAPALRAATGATVLGRAAPATQWQDIAFAPA
RDLQHGERIAIGDDCTLRVIHTPGHASNHLCFLLEEEKTLFTGDHVMQGSTVVISPPDGDMRAYLDSLAALQEEDLEWLA
PGHGFLITRPQEAIRLLVRHRLHREAKVLSALRDLGPAPIEALLPRVYDDVPERMYPVAQRSLHAHLLKLVADGKAGDTD
GRWQAV
>Mature_565_residues
TTTETSTTTNRPASPSKSLRAAASLLVVRDGPQGMEVLLVRRVERANDRSSGAFVFPGGTLDPEQDKALHIHAAGLDDTA
ASERLNLPANGLDFYLAAVRECFEEAGLLLAYHRNGDLLAPGHLDDAQLDFLRQAVQRGGPGLAHACAELGLRLASDRLA
YHSYWLTPPGLPKRFDTRFFVAIAPAGQIAIHDGMEIVEHRWIRPGEAADPASGLPMMHVTRRTLQSIAHFETAQECFAS
TSLLRDIACIMPRLANGAAGVRPVMPNESCYAEIGRIDPEGKTHARYELAPGEPVRLSERVWRVTANNGSVMTGPGTNTY
LVGGGARNEWAVIDPGPDDAAHVQAVLAAAPGPIRWILATHTHMDHSPAAPALRAATGATVLGRAAPATQWQDIAFAPAR
DLQHGERIAIGDDCTLRVIHTPGHASNHLCFLLEEEKTLFTGDHVMQGSTVVISPPDGDMRAYLDSLAALQEEDLEWLAP
GHGFLITRPQEAIRLLVRHRLHREAKVLSALRDLGPAPIEALLPRVYDDVPERMYPVAQRSLHAHLLKLVADGKAGDTDG
RWQAV

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI7705793, Length=284, Percent_Identity=32.3943661971831, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1787158, Length=179, Percent_Identity=31.2849162011173, Blast_Score=66, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17537503, Length=269, Percent_Identity=29.368029739777, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI193205216, Length=269, Percent_Identity=29.368029739777, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI20129357, Length=275, Percent_Identity=32, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 61297; Mature: 61166

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTETSTTTNRPASPSKSLRAAASLLVVRDGPQGMEVLLVRRVERANDRSSGAFVFPGG
CCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCC
TLDPEQDKALHIHAAGLDDTAASERLNLPANGLDFYLAAVRECFEEAGLLLAYHRNGDLL
CCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEE
APGHLDDAQLDFLRQAVQRGGPGLAHACAELGLRLASDRLAYHSYWLTPPGLPKRFDTRF
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEE
FVAIAPAGQIAIHDGMEIVEHRWIRPGEAADPASGLPMMHVTRRTLQSIAHFETAQECFA
EEEECCCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
STSLLRDIACIMPRLANGAAGVRPVMPNESCYAEIGRIDPEGKTHARYELAPGEPVRLSE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEECC
RVWRVTANNGSVMTGPGTNTYLVGGGARNEWAVIDPGPDDAAHVQAVLAAAPGPIRWILA
EEEEEECCCCEEEECCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEE
THTHMDHSPAAPALRAATGATVLGRAAPATQWQDIAFAPARDLQHGERIAIGDDCTLRVI
ECCCCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCEECCCCHHCCCCCEEEECCCCEEEEE
HTPGHASNHLCFLLEEEKTLFTGDHVMQGSTVVISPPDGDMRAYLDSLAALQEEDLEWLA
ECCCCCCCCEEEEEECCCEEEECCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHEEC
PGHGFLITRPQEAIRLLVRHRLHREAKVLSALRDLGPAPIEALLPRVYDDVPERMYPVAQ
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RSLHAHLLKLVADGKAGDTDGRWQAV
HHHHHHHHHHHHCCCCCCCCCCEECC
>Mature Secondary Structure 
TTTETSTTTNRPASPSKSLRAAASLLVVRDGPQGMEVLLVRRVERANDRSSGAFVFPGG
CCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCC
TLDPEQDKALHIHAAGLDDTAASERLNLPANGLDFYLAAVRECFEEAGLLLAYHRNGDLL
CCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEE
APGHLDDAQLDFLRQAVQRGGPGLAHACAELGLRLASDRLAYHSYWLTPPGLPKRFDTRF
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEE
FVAIAPAGQIAIHDGMEIVEHRWIRPGEAADPASGLPMMHVTRRTLQSIAHFETAQECFA
EEEECCCCCEEEECCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
STSLLRDIACIMPRLANGAAGVRPVMPNESCYAEIGRIDPEGKTHARYELAPGEPVRLSE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEECC
RVWRVTANNGSVMTGPGTNTYLVGGGARNEWAVIDPGPDDAAHVQAVLAAAPGPIRWILA
EEEEEECCCCEEEECCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEE
THTHMDHSPAAPALRAATGATVLGRAAPATQWQDIAFAPARDLQHGERIAIGDDCTLRVI
ECCCCCCCCCCCHHHHHCCCHHHCCCCCCCCCCCEECCCCHHCCCCCEEEECCCCEEEEE
HTPGHASNHLCFLLEEEKTLFTGDHVMQGSTVVISPPDGDMRAYLDSLAALQEEDLEWLA
ECCCCCCCCEEEEEECCCEEEECCEEECCCEEEEECCCCHHHHHHHHHHHHHHCCHHEEC
PGHGFLITRPQEAIRLLVRHRLHREAKVLSALRDLGPAPIEALLPRVYDDVPERMYPVAQ
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RSLHAHLLKLVADGKAGDTDGRWQAV
HHHHHHHHHHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA