| Definition | Ralstonia eutropha JMP134 chromosome chromosome 2, complete sequence. |
|---|---|
| Accession | NC_007348 |
| Length | 2,726,152 |
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The map label for this gene is metE [H]
Identifier: 73538075
GI number: 73538075
Start: 898639
End: 901041
Strand: Direct
Name: metE [H]
Synonym: Reut_B4245
Alternate gene names: 73538075
Gene position: 898639-901041 (Clockwise)
Preceding gene: 73538073
Following gene: 73538076
Centisome position: 32.96
GC content: 67.21
Gene sequence:
>2403_bases GTGCGCCAGACAAGACCTTTTTGGAGCATCTTCGTGGCACGCACACACATCCTCGGCTTTCCGCGCATCGGCGCTCGTCG TGAACTGAAATTTGCACAGGAATCCTTCTGGCGCGGGGAAAGCACGGAAGCGGACCTGCGCGCCGTGGCCGCCACGCTGC GCAGCCGCCACTGGCAATTGCAGGCTGACAGCGGTCTGGATACCGTTGCTACAGGCGACTTCGCCTACTACGACCAGGTG CTGAGCCTCACGGCGCTGCTCGGTGCGCTGCCGCGCCGCTTCGGCTTCGAGCCGGCCGCGCTGACGCTGGCCCAGTATTT CGAGCTCGCACGCGGCAATCGCGAACAGCCCGCAATGGAAATGACCAAGTGGTTCGATACGAACTACCACTACCTGGTGC CGGAGCTGGACTCGGCCACGCGCTTCGACGGCGGCCCCGACTGGTTTTTCGACGAACTCGAAGAAGCGCTGGCGCTCGGC CTGAAGGCGCGGCCGGTGCTGATCGGGCCCGTGACCTACCTGTGGCTGTCCAAGACGCACGAGGCCGGGTTTGACAAGCT GTCGCTCCTCCCGCAACTCGCGCAGGCTTACCAGCGCATCCTTACCCGCCTGAAGGCGCGTGGTATCGATTGGGTGCAGA TCGACGAGCCCGCGCTGAGCCTCGACCTCGACGCGGCCTGGCTCGATGCCTACGACCGGGCGTACGCGACCCTGGCCCAG TCGGGCGTGAAACTGCTGCTCGCAACCTACTTCGACAGCGCAGCCGACCATGCCGAGCGTGCGGCCGCGCTGCCAGTCCA CGGCTTCCATATCGACGTGGTGCGGGCGCCCGCGCAGCTTGTTGCATGGCGCCAGGCGCTGCCGCCCGAGGCCGTGCTGT CCGTTGGCGTGATCGACGGCCGCAATATCTGGCGCACCGACCTGCGCCGCGCGCTCGACACGCTGAAACCGCTGCATGCC GAACTCGGCGATCGGCTGTGGCTGGCACCGTCGTGCTCGCTGCTGCATGTGCCCGTATCCCTCGAACATGAAGTCAGGCT CGATGCCGAGCTGAAGTCCTGGCTGGCCTTTGCCACCGAAAAGCTGGGCGAACTGTGTGTGCTGGCCACGGTGCTGAATC AAGGCGAGACAGCCGTCGCCGCCCAGCTCGTGGCTTCCGATGCCGTGCAGGCGTCGCGCCGCCAATCGCGCCGCGTGGTC AATCCGCGCGTACAGCAGCGGCTGGCTGCTGTGACGCCTGACATGGCCGACCGCGACAGCGCGTTCGATGTCCGCATCGA ACGTCAGCGCGAGGCCCTGCAACTGCCGCTGCTCCCGACCACGACGATTGGCTCGTTCCCGCAGACGGCCGCCATCCGCC AGACCCGGGCGGCCTACAAACGCGGCGATCTCGGCGCGCTCGACTACCTGCACCGCATCCGCGGCGAAATTGAGCTGGCC GTGCGCAAGCAGGAAGCGCTGGGGCTGGACGTGCTGGTGCACGGCGAAGCCGAGCGCAACGACATGGTCGAATACTTCGG CGAGCAGCTGTGCGGCTATGCGTTCACCGAGAACGGCTGGGTGCAGAGCTACGGCTCGCGCTGCGTCAAGCCCCCTGTGA TCTACGGCGACGTGTACCGGCCTGAGCCAATGACCGTGGACACTGCGCGCTATGCCCAGTCGCTGACCGAGCGTCCGATG AAGGGCATGCTGACCGGCCCCGTCACGATGCTGCAGTGGTCGTTCGTCCGCGACGACCAGCCGCGCGCCGATACCGCCCG CCAACTCGCACTGGCCATCCGCGACGAAGTGTGTGACCTGGAACAGGCCGGCATCCGCGTGATCCAGATCGACGAGCCGG CGCTGCGCGAGGGCCTGCCGCTGCGCCGTGCCGACTGGGACGCCTACCTGGCATGGGCCGTCAACGCGTTCCGGCTGTCG GCCGCGGGCGTGGCGGACCAGACCCAGATCCATACCCATATGTGCTATGCCGAGTTCAACGACATCCTGCCGTCGATCGC GGCGATGGATGCGGACGTGATCACCATCGAGACCTCGCGCTCGGCCATGGAACTGCTCGAAGGCTTTGGCGATTTCGACT ACCCGAACGAGATCGGACCGGGCGTCTACGACATCCACTCGCCGCGCGTGCCGTCGGTGGAAGCCATGGAAAAGCTGCTG GACCGCGCCTGCGAGGTCGTGCCGCCGCAGCGTCTGTGGGTCAACCCGGACTGCGGCCTGAAGACCCGCGGTTGGGATGA GACCGAGGCCGCCCTGGCCAATATGGTGTCGGCGGCGCGCAAGTTGCGCGGGCGCCTCGCGGGTCAGGACACGCTGACGT GGAAGCGTGTGGCACGGCCGCAGGCGTCACAGGCGGCACATCGGCACGGCGCCGAGTGCGCCTGCAGCACCCGCGCCGCC TGA
Upstream 100 bases:
>100_bases GTGACCCTGCCCGTTTCATTGTGGCTTCGGGTGATATGAAATTCATTCATACTTTGTTTAAAACTTGTCGCTTGATTCAT TTTACGGTGAAACCGACACT
Downstream 100 bases:
>100_bases GCGCAGCCGCCTTGTGACGTGTGCGCCGCGCGTGCGGCGCTGTGTCACATAGCCGAGGCGTGCTGCGCTCCGTATCCTTG CCGGCTATCGAATTACACTC
Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase
Products: NA
Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]
Number of amino acids: Translated: 800; Mature: 800
Protein sequence:
>800_residues MRQTRPFWSIFVARTHILGFPRIGARRELKFAQESFWRGESTEADLRAVAATLRSRHWQLQADSGLDTVATGDFAYYDQV LSLTALLGALPRRFGFEPAALTLAQYFELARGNREQPAMEMTKWFDTNYHYLVPELDSATRFDGGPDWFFDELEEALALG LKARPVLIGPVTYLWLSKTHEAGFDKLSLLPQLAQAYQRILTRLKARGIDWVQIDEPALSLDLDAAWLDAYDRAYATLAQ SGVKLLLATYFDSAADHAERAAALPVHGFHIDVVRAPAQLVAWRQALPPEAVLSVGVIDGRNIWRTDLRRALDTLKPLHA ELGDRLWLAPSCSLLHVPVSLEHEVRLDAELKSWLAFATEKLGELCVLATVLNQGETAVAAQLVASDAVQASRRQSRRVV NPRVQQRLAAVTPDMADRDSAFDVRIERQREALQLPLLPTTTIGSFPQTAAIRQTRAAYKRGDLGALDYLHRIRGEIELA VRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPM KGMLTGPVTMLQWSFVRDDQPRADTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLAWAVNAFRLS AAGVADQTQIHTHMCYAEFNDILPSIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVEAMEKLL DRACEVVPPQRLWVNPDCGLKTRGWDETEAALANMVSAARKLRGRLAGQDTLTWKRVARPQASQAAHRHGAECACSTRAA
Sequences:
>Translated_800_residues MRQTRPFWSIFVARTHILGFPRIGARRELKFAQESFWRGESTEADLRAVAATLRSRHWQLQADSGLDTVATGDFAYYDQV LSLTALLGALPRRFGFEPAALTLAQYFELARGNREQPAMEMTKWFDTNYHYLVPELDSATRFDGGPDWFFDELEEALALG LKARPVLIGPVTYLWLSKTHEAGFDKLSLLPQLAQAYQRILTRLKARGIDWVQIDEPALSLDLDAAWLDAYDRAYATLAQ SGVKLLLATYFDSAADHAERAAALPVHGFHIDVVRAPAQLVAWRQALPPEAVLSVGVIDGRNIWRTDLRRALDTLKPLHA ELGDRLWLAPSCSLLHVPVSLEHEVRLDAELKSWLAFATEKLGELCVLATVLNQGETAVAAQLVASDAVQASRRQSRRVV NPRVQQRLAAVTPDMADRDSAFDVRIERQREALQLPLLPTTTIGSFPQTAAIRQTRAAYKRGDLGALDYLHRIRGEIELA VRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPM KGMLTGPVTMLQWSFVRDDQPRADTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLAWAVNAFRLS AAGVADQTQIHTHMCYAEFNDILPSIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVEAMEKLL DRACEVVPPQRLWVNPDCGLKTRGWDETEAALANMVSAARKLRGRLAGQDTLTWKRVARPQASQAAHRHGAECACSTRAA >Mature_800_residues MRQTRPFWSIFVARTHILGFPRIGARRELKFAQESFWRGESTEADLRAVAATLRSRHWQLQADSGLDTVATGDFAYYDQV LSLTALLGALPRRFGFEPAALTLAQYFELARGNREQPAMEMTKWFDTNYHYLVPELDSATRFDGGPDWFFDELEEALALG LKARPVLIGPVTYLWLSKTHEAGFDKLSLLPQLAQAYQRILTRLKARGIDWVQIDEPALSLDLDAAWLDAYDRAYATLAQ SGVKLLLATYFDSAADHAERAAALPVHGFHIDVVRAPAQLVAWRQALPPEAVLSVGVIDGRNIWRTDLRRALDTLKPLHA ELGDRLWLAPSCSLLHVPVSLEHEVRLDAELKSWLAFATEKLGELCVLATVLNQGETAVAAQLVASDAVQASRRQSRRVV NPRVQQRLAAVTPDMADRDSAFDVRIERQREALQLPLLPTTTIGSFPQTAAIRQTRAAYKRGDLGALDYLHRIRGEIELA VRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPM KGMLTGPVTMLQWSFVRDDQPRADTARQLALAIRDEVCDLEQAGIRVIQIDEPALREGLPLRRADWDAYLAWAVNAFRLS AAGVADQTQIHTHMCYAEFNDILPSIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVEAMEKLL DRACEVVPPQRLWVNPDCGLKTRGWDETEAALANMVSAARKLRGRLAGQDTLTWKRVARPQASQAAHRHGAECACSTRAA
Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]
COG id: COG0620
COG function: function code E; Methionine synthase II (cobalamin-independent)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]
Homologues:
Organism=Escherichia coli, GI2367304, Length=754, Percent_Identity=58.0901856763926, Blast_Score=865, Evalue=0.0, Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=46.1638491547464, Blast_Score=674, Evalue=0.0,
Paralogues:
None
Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013215 - InterPro: IPR006276 - InterPro: IPR002629 [H]
Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]
EC number: =2.1.1.14 [H]
Molecular weight: Translated: 89346; Mature: 89346
Theoretical pI: Translated: 5.78; Mature: 5.78
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 1.8 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRQTRPFWSIFVARTHILGFPRIGARRELKFAQESFWRGESTEADLRAVAATLRSRHWQL CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEE QADSGLDTVATGDFAYYDQVLSLTALLGALPRRFGFEPAALTLAQYFELARGNREQPAME ECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHH MTKWFDTNYHYLVPELDSATRFDGGPDWFFDELEEALALGLKARPVLIGPVTYLWLSKTH HHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHH EAGFDKLSLLPQLAQAYQRILTRLKARGIDWVQIDEPALSLDLDAAWLDAYDRAYATLAQ HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHH SGVKLLLATYFDSAADHAERAAALPVHGFHIDVVRAPAQLVAWRQALPPEAVLSVGVIDG HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHEEECCCC RNIWRTDLRRALDTLKPLHAELGDRLWLAPSCSLLHVPVSLEHEVRLDAELKSWLAFATE CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEECHHHHHHHHHHHH KLGELCVLATVLNQGETAVAAQLVASDAVQASRRQSRRVVNPRVQQRLAAVTPDMADRDS HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC AFDVRIERQREALQLPLLPTTTIGSFPQTAAIRQTRAAYKRGDLGALDYLHRIRGEIELA CEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE VRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYR EEHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCCEEECCCCC PEPMTVDTARYAQSLTERPMKGMLTGPVTMLQWSFVRDDQPRADTARQLALAIRDEVCDL CCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHCH EQAGIRVIQIDEPALREGLPLRRADWDAYLAWAVNAFRLSAAGVADQTQIHTHMCYAEFN HHCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH DILPSIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVEAMEKLL HHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHH DRACEVVPPQRLWVNPDCGLKTRGWDETEAALANMVSAARKLRGRLAGQDTLTWKRVARP HHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC QASQAAHRHGAECACSTRAA CHHHHHHHCCCCCCCCCCCC >Mature Secondary Structure MRQTRPFWSIFVARTHILGFPRIGARRELKFAQESFWRGESTEADLRAVAATLRSRHWQL CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEE QADSGLDTVATGDFAYYDQVLSLTALLGALPRRFGFEPAALTLAQYFELARGNREQPAME ECCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHH MTKWFDTNYHYLVPELDSATRFDGGPDWFFDELEEALALGLKARPVLIGPVTYLWLSKTH HHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHH EAGFDKLSLLPQLAQAYQRILTRLKARGIDWVQIDEPALSLDLDAAWLDAYDRAYATLAQ HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHH SGVKLLLATYFDSAADHAERAAALPVHGFHIDVVRAPAQLVAWRQALPPEAVLSVGVIDG HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHEEECCCC RNIWRTDLRRALDTLKPLHAELGDRLWLAPSCSLLHVPVSLEHEVRLDAELKSWLAFATE CCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEECHHHHHHHHHHHH KLGELCVLATVLNQGETAVAAQLVASDAVQASRRQSRRVVNPRVQQRLAAVTPDMADRDS HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC AFDVRIERQREALQLPLLPTTTIGSFPQTAAIRQTRAAYKRGDLGALDYLHRIRGEIELA CEEEEECCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEE VRKQEALGLDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYR EEHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCCEEECCCCC PEPMTVDTARYAQSLTERPMKGMLTGPVTMLQWSFVRDDQPRADTARQLALAIRDEVCDL CCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHCH EQAGIRVIQIDEPALREGLPLRRADWDAYLAWAVNAFRLSAAGVADQTQIHTHMCYAEFN HHCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH DILPSIAAMDADVITIETSRSAMELLEGFGDFDYPNEIGPGVYDIHSPRVPSVEAMEKLL HHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHH DRACEVVPPQRLWVNPDCGLKTRGWDETEAALANMVSAARKLRGRLAGQDTLTWKRVARP HHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC QASQAAHRHGAECACSTRAA CHHHHHHHCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA